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Biomedical subjects

L G Wayne

Publications and source records attributed to L G Wayne.

At least 55 records · Page 3Linked to original sources
PubMed1972

Purification of mycobacterial deoxyribonucleic acid.

Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be the same for all strains studied. Precipitation of DNA with cetyltrimethylammonium bromide was used to separate impurities from some DNA preparations. The presence of the contaminants was found to affect markedly the determination of the guanine plus cytosine content according to a method dependent on the ratio of absorbancies at 260 and 280 nm; the impurities did not affect the determination by the method of thermal denaturation. The presence of a DNA-polysaccharide complex is suggested.

Bacillus subtilis↗
PubMed1971

Phenol-Soluble Antigens from Mycobacterium kansasii, Mycobacterium gastri, and Mycobacterium marinum.

Many of the demonstrable antigens derived from mycobacteria are common to members of different species. Agglutination tests have yielded the most specific characterizations. An antigen which may be associated with the specific agglutination is soluble in phenol and can be extracted and separated from other antigens by use of this solvent. Phenol-soluble antigens have been extracted from representative cultures of Mycobacterium kansasii, M. gastri, and M. marinum. Most representatives of each of these species yielded an antigen which was characteristic of the species but was distinct from antigen derived from the other two species.

Journal Article↗
PubMed1970

Nucleic acid homology in the genus Mycobacterium.

Immobilized deoxyribonucleic acid (DNA) preparations from eight species of mycobacteria were reacted with labeled reference DNA from Mycobacterium tuberculosis and M. kansasii. All mycobacteria showed some degree of homology. Immobilized DNA from Pseudomonas multivorans, which has a guanine plus cytosine content within the range established for the mycobacteria, showed no significant binding with either reference system. Relative per cent binding and thermal stability of bound DNA were used as parameters for determining degrees of relatedness among members of the genus. When these relationships were compared with those established by numberical taxonomic methods, a high correlation was found.

Carbon Isotopes↗
PubMed1969

Comparison of methods for tuberculosis bacteriology.

To improve efficiency of isolation of tubercle bacilli from clinical specimens, the following recommendations are presented. (i) Employ multiple specimens consisting of a combination of morning sputums for the early detection of positives, along with 24-hr sputum pools for the greatest total yield of positives. (ii) When timing is rigorously controlled, Zephiran-trisodium phosphate and sodium hydroxide-acetylcysteine are comparable, but if timing cannot rigidly be controlled, employ the Zephiran-trisodium phosphate digestion procedure to allow the greatest freedom in exposure time with the lowest kill rate to tubercle bacilli. (iii) Employ both an agar medium incubated in 5% CO(2), for the early detection of positives as well as positives in the presence of contaminants, and an egg medium, preferably with CO(2), to increase the yield of positives.

Agar↗
PubMed1968

Diagnostic key to mycobacteria encountered in clinical laboratories.

A diagnostic key has been developed which will permit identification of most mycobacteria encountered in clinical laboratories. The key is based on performance of a few simple tests. The efficiency and accuracy of the key was evaluated in terms of correlation between identifications based on the few tests and those arrived at through application of the techniques of numerical taxonomy, which involves a large battery of tests. Of 679 cultures of mycobacteria other than Mycobacterium tuberculosis, 86.5% were correctly identified by use of the key, and only 1.8% of the cultures were erroneously identified. The remaining cultures required further examination.

Bacteriological Techniques↗
PubMed1968

Base composition of deoxyribonucleic acid isolated from mycobacteria.

Guanine plus cytosine values of deoxyribonucleic acid derived from 30 cultures representing 14 mycobacterial species or varieties are presented. These data provide impressive reasons for maintaining the separation between the genera Corynebacterium and Mycobacterium; no conclusions can be arrived at from these data with respect to the Nocardia-Mycobacterium relationship. A bimodal clustering, in terms of guanine plus cytosine composition, is apparent within the genus Mycobacterium. In general, all members of any single phenetic species appear to fit into one or another of these clusters. The phenetic separation of species is, in some cases, confirmed by separation in terms of guanine plus cytosine values. The bimodal separation of guanine plus cytosine values within the genus Mycobacterium does not correspond to a division of the species into slow and rapid growers; it thus provides no justification for splitting Mycobacterium into two genera, composed of slow and rapid growers. This is not to say that such a split would not be useful, only that these data do not contribute to such a decision. Any further attempts to correlate phenetic classification with properties of mycobacterial deoxyribonucleic acid will require more specific techniques, such as molecular hybridization.

Corynebacterium↗