Search PubMed⌕ Search

Biomedical subjects

L Freitas

Publications and source records attributed to L Freitas.

At least 19 recordsLinked to original sources
PubMed2005

Carcinoma ex pleomorphic adenoma (CXPA): immunoprofile of the cells involved in carcinomatous progression.

AIMS: To characterize the cellular component in pleomorphic adenoma (PA) that undergoes malignant transformation in carcinoma ex pleomorphic adenoma (CXPA). METHODS AND RESULTS: A panel of antibodies against cytoskeletal proteins was applied in 16 cases of CXPA: intracapsular carcinoma (five cases), minimally invasive (four cases) and frankly invasive (seven cases). The CXPAs were classified into two main groups according to their predominant cellular component as detected by the panel of antibodies: (i) carcinomas with only epithelial differentiation (75% of the cases), and (ii) carcinomas with a myoepithelial component (25%). CXPA with only epithelial differentiation showed two types of malignant areas in the part of the tumour that was confined by the PA capsule: (i) intraductal carcinoma areas characterized by ductal structures containing both benign myoepithelial cells positive for alpha-smooth muscle actin (alpha-SMA), vimentin and cytokeratin (CK)14 and proliferating atypical luminal cells reactive for CK7, CK8 and CK19, and (ii) carcinoma areas composed only of epithelial cells reactive for CK7, CK8 and CK19. In the latter, the cells presented morphological and immunohistochemical characteristics similar to those found in areas of invasive carcinoma outside the PA capsule. CXPAs with a myoepithelial component were composed mainly or exclusively of cells that expressed vimentin and alpha-SMA. In this group, ductal structures reminiscent of PA filled by malignant cells were not identified. CONCLUSION: Most CXPAs consist only of epithelial cells that have an immunoprofile comparable to ductal luminal cells of PA. These malignant luminal cells arise in the duct-like structures as intraductal carcinoma and probably only at this early stage of development should the lesion be considered as a non-invasive carcinoma.

Actins↗
PubMed2005

Maspin expression in carcinoma ex pleomorphic adenoma.

AIMS: To investigate the presence and distribution of the protein maspin in carcinoma ex pleomorphic adenoma (CXPA). METHODS: Maspin expression was studied by means of immunohistochemistry in 16 cases of CXPA, using the labelled polymer method. RESULTS: According to the extent of invasion, the tumours were subdivided into: intracapsular (five cases), minimally invasive (four cases), and invasive (seven cases). Twelve patients had carcinoma with only epithelial differentiation, whereas four had a malignant myoepithelial component. Non-luminal cells in the duct-like structures of the remnant pleomorphic adenoma were strongly positive for maspin, whereas only a few luminal cells were immunopositive. A few positive cells were seen in the frequent hypocellular and hyalinised areas. Maspin was abundantly expressed, mainly in non-luminal cells, in transitional areas of CXPA with only epithelial differentiation. In frankly carcinomatous areas there was a gradual decrease in maspin expression. Almost all cells were maspin positive in CXPA with a myoepithelial component. When present, luminal cells were in general negative for maspin. CONCLUSIONS: When only epithelial cells undergo malignant transformation, maspin expression is gradually lost. In cases with a myoepithelial component, maspin expression is high, and this might be related to the tumour suppressor activity attributed to this cell.

Adenocarcinoma↗
PubMed1999

Primary antiphospholipid syndrome presented by total infarction of right kidney with nephrotic syndrome.

We report the case of a young woman with primary antiphospholipid syndrome (APS), which presented with acute renal failure, hypoproteinemia, hypoalbuminemia and nephrotic proteinuria. Investigations showed total infarction of right kidney by extensive arterial and vein thrombosis and presence of anticardiolipin antibodies IgG isotype (anti-beta2-glycoprotein I-positive). She was submitted to right nefrectomy and initiated anticoagulant therapy. After nefrectomy, the postoperative period was marked by the development of arterial hypertension and persistence of nephrotic syndrome. Hypertension was treated with antihypertensive drugs (IECA, beta-blocker and calcium antagonist). As the nephrotic syndrome persisted despite anticoagulant and antihypertensive therapy, the patient was treated with oral corticosteroids. Her renal function improved, hypoproteinemia and hypoalbuminemia corrected to normal values and proteinuria decreased to subnephrotic value. We discuss the unusual presentation of this case of primary antiphospholipid syndrome with total unilateral renal thrombosis and nephrotic syndrome that respond to anticoagulant, antihypertensive and corticosteroid therapy.

Acute Kidney Injury↗
PubMed1992

[Emphysematous cholecystitis].

Emphysematous cholecystitis (EC) is a rare and dramatic disease that requires prompt therapeutic procedures. The authors report a case of a 70-year-old-woman, admitted to the Intensive Care Unit for Infectious Diseases of the Santa Maria Hospital, with the diagnosis of EC. The literature on EC is also reviewed.

Aged↗
PubMed1992

[Febrile coma and disseminated intravascular coagulation following heat stroke].

A clinical case that occurred in a 42 years old female and which fulfils the diagnostic criteria for the entity described as Heat Stroke is presented. In this case, besides the usual manifestations of hyperpyrexia without sweating along with consciousness disorders, features of consumptive coagulopathy, rhabdomyolysis and well marked laboratory liver dysfunction have also been found. The severity of this situation and its less common occurrence in temperate climates, together with the uncertainty in establishing the diagnosis, namely when infection is suspected--in the present case the initial diagnosis was Sepsis, which led to patient's admission in an infectious diseases intensive care unit--motivate the authors to make this report. Furthermore, they consider this case to be a good example of the varied clinical and laboratory manifestations and possible severe complications that Heat Stroke may display.

Adult↗
PubMed1990

Doing history.

Explore the source record for details and available documents.

Bias↗
PubMed1988

[Lower intestinal obstruction caused by a lactobezoar].

Two case of the so-called "milk curd obstruction" syndrome are reported. Although seldom found elsewhere than in the stomach, more distal obstruction in the ileum or the transverse colon by milk curd aggregates may mimic more classical causes of obstruction if one is not aware of the possibility of such incident related to feeding. Appropriate interviewing of the mother about regimen and medications will be of great help, in investigating the cause of the abdominal disorder, obviating the need for surgical intervention if confirmed by radiopaque enema.

Bezoars↗
PubMed1987

Second operation for repair of biliary atresia.

In our experience with biliary atresia, there are few cases amenable to reoperation for recurrent jaundice. All authors would agree that specific conditions such as complete bile flow recovery from the first operation followed by early recurrence should be an unquestionable case for revision of the anastomosis, inasmuch as no biologic signs of ongoing cholangitis can be traced. The same decision would apply to the problem of bile leakage after hepatoportocholecystostomy. In other cases, however, one should be aware that these reoperations expose the child to ascitis, poor healing of the abdominal wound, liver failure, and also bring with the decision to reoperate undue hopes to the parents of the child. Moreover, if the child should be a future candidate for liver transplantation, it may be wiser to avoid useless laparotomies and abdominal dissections that are known to complicate the task of hepatectomy.

Biliary Atresia↗
PubMed1987

[Management of uropathies diagnosed prenatally. Discussion based on a series of 53 cases].

From 1982 to 1986, 53 newborns (26 boys and 27 girls) were referred to the authors for the management of a congenital anomaly of the urinary tract, following a prenatal ultrasonographic diagnosis. The postnatal diagnosis was hydronephrosis in 27 children (10/27 bilateral cases), unilateral multicystic dysplasia in 11, ureteral duplication in 6, primary megaureter or orthotopic ureterocele in 5 (1/5 bilateral case) and posterior urethral valves in 4. An early urinary tract infection was noticed in 5 cases only and 2 boys with urethral valves had an altered renal function at birth. Eight children with a mild lesion were not operated. A radical procedure was performed in 15 cases: excision of a multicystic kidney (10 cases) or heminephrectomy of an upper non-functioning pyelon (5 cases: 3 with heterotopic ureterocele and 2 with ectopic ureter). Thirty children were submitted to a corrective procedure: electrocoagulation of urethral valves (4 cases), ureteroneocystostomy (6 cases) or pyeloplasty (19 unilateral and 1 bilateral procedure). Except in a case of pyeloplasty the result of the reconstructive surgery was considered as good or satisfactory from a radiological point of view, with a mean follow-up of 1.5 year. The essential point of discussion is the evaluation of the factors which must be taken in account to plan an early reconstructive surgical treatment. The main factor, I.e. the natural history of these congenital anomalies remains at yet difficult to predict in a great number of cases.(ABSTRACT TRUNCATED AT 250 WORDS)

Female↗
PubMed1976

Banding patterns of the chromosomes of Cebus albifrons. Comparative study with Cebur apella.

Quinacrine- and Giemsa-banding studies of the chromosomes of Cebus albifrons permitted to obtain a pattern that characterizes the species. The topography of the bands has been compared with that of Cebus apella. Each chromosome pair of C. albifrons has a homologue in C. apella. The differences between the two karyotypes are the result of five pericentric inversions.

Animals↗