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Biomedical subjects

L Carlsson

Publications and source records attributed to L Carlsson.

187 records · Page 11Linked to original sources
PubMedDate not supplied

Local release of myocardial norepinephrine during acute ischemia: an experimental study in the isolated perfused rat heart.

The appearance of an early ischemia-induced local release of myocardial norepinephrine (NE) was examined in the isolated Langendorff-perfused rat heart prelabeled with [3H]NE. Either glucose or lactate was used as the perfusion substrate. Ischemia for a 60-min period was produced by global flow reduction (by 90%) or left coronary artery ligation followed by a 15-min reperfusion period. During the first 20 min of ischemia both the concentration of [3H]NE and the fraction of total 3H representing nonmetabolized [3H]NE were increased in the coronary venous effluent. This early increase in [3H]NE concentration was most pronounced in hearts with global ischemia perfused with lactate as substrate (from 19 +/- 2 to 524 +/- 76 fmol/ml/g after 20 min of ischemia). The quantity of [3H]NE released was then further increased during the 60-min period of ischemia. Reperfusion of the ischemic myocardium was associated with a marked outflow of [3H]NE and [3H]NE metabolites, primarily representing a washout from the ischemic tissue. Under the present experimental conditions the ischemia-induced release of NE was attenuated by glucose, probably owing to an ongoing glycolytic ATP formation. This effect was most pronounced during global low-flow ischemia. It is concluded that ischemia is associated with a local release of myocardial NE. In the nonworking Langendorff heart preparation, clear evidence of such a local release was already obtained after 10-20 min of ischemia.

Animals↗
PubMedDate not supplied

Release of noradrenaline in myocardial ischemia--importance of local inactivation by neuronal and extraneuronal mechanisms.

The mechanisms responsible for the local inactivation of noradrenaline (NA) released during myocardial ischemia were examined in the isolated perfused rat heart preloaded with 3H-NA. Two different types of ischemia were used: (a) 90% global flow reduction (global ischemia), and (b) ligation of the left coronary artery (regional ischemia). The coronary effluent was collected at different intervals during ischemia and reperfusion, and 3H-NA was separated from its tritiated metabolites using HPLC. During ischemia, a gradual increase in the efflux of 3H-NA was observed, which was accompanied by a shift from predominantly neuronal metabolism towards increased efflux of extraneuronally formed metabolites. During the first minute of reperfusion, the metabolic pattern of 3H-NA was not substantially changed as compared with that observed immediately before reperfusion, indicating a wash-out of 3H-NA and its tritiated metabolites accumulated in the tissue during the ischemic period. Addition of corticosterone to the perfusion medium (to inhibit extraneuronal uptake of 3H-NA) was associated with an increased efflux of 3H-NA during the ischemic period. In contrast, addition of the neuronal uptake blocker desipramine caused a decreased efflux of 3H-NA during ischemia. In conclusion, these experiments demonstrate an ischemia-induced release of myocardial NA, in all probability due to the recently proposed carrier-mediated efflux mechanism. The release is parallelled by an increasing extraneuronal inactivation of released transmitter.

Animals↗
PubMedDate not supplied

Motility stimulant effects of prostasome inclusion in swim-up medium on cryopreserved human spermatozoa.

The chance of obtaining a fertilization and establishing a pregnancy increases with the number of motile spermatozoa that can reach and interact with the oocyte after the time of insemination. In an attempt to increase the recovery of freeze-thawed and motile spermatozoa, the swim-up medium was supplemented with prostasomes and some other effectors. Swim-up media supplemented with prostasomes were superior in comparison to the other effectors investigated in the recovery of motile spermatozoa for insemination. These results suggest that prostasome inclusion in swim-up medium might be of benefit in improving results in assisted reproductive technologies using freeze-thawed spermatozoa.

Aminopeptidases↗
PubMedDate not supplied

A decision support system for predicting a recurrence of breast cancer; a prospective study of serum tumour markers TAG 12, CA 15-3 and MCA.

The aim of the present study was to evaluate the clinical value of the preoperative serum tumour markers TAG 12, CA 15-3 and MCA in predicting a recurrence of breast cancer patients. The sensitivity of the TAG 12 test was 54%, that of the CA 15-3 test 15% and that of the MCA test 15% in predicting a recurrence of breast cancer. The most important predictor of breast cancer recurrence was TAG 12. In order to evaluate the contributions of different tumour marker serum test, a stepwise discriminant analysis was carried out. The discriminant function (DF) is DF = TAG 12 x 0.061 - CA 15-3 x 0.1336 - 0.396. The sensitivity of the DF in detecting recurrence of breast cancer was 63% with a specificity of 90% and an efficiency of 75%. In conclusion, the results indicate that a new tumour marker TAG 12 is superior to CA 15-3 and MCA in predicting breast cancer recurrence. In this study the discriminant function including TAG 12 and CA 15-3 was superior to single preoperative tumour marker tests. The results speak for the use of a decision support system to aid in predicting a recurrence of breast cancer.

Antigens, Neoplasm↗
PubMedDate not supplied

Comparison of tumour markers TAG 12 with CA 15-3 and MCA in breast cancer diagnosis.

The aim of the present study was to evaluate the clinical value of the new tumour marker TAG 12 in sera from patients with breast cancer (n = 123), patients with benign breast disease (n = 30) and normal controls (n = 38). The results for TAG 12 were compared with those for CA 15-3 and MCA. The cut-off levels (90% specificity) determined for each test in the benign and control groups were 57.5 kU/1 for TAG 12, 24.8 kU/1 for CA 15-3 and 11.8 kU/1 for MCA. The diagnostic sensitivity of the TAG 12 test was 0.30, 0.19 for CA 15-3 test and 0.20 for MCA test in detecting breast cancer. TAG 12, CA 15-3 and MCA tests were tested in a multivariate analysis to find the best combination of independent predictors of breast cancer. The most important predictor of breast cancer was TAG 12. In order to evaluate the contributions of different tumour marker serum tests a stepwise discriminant analysis was carried out. The discriminant function is DF = TAG 12 x 0.0349 + CA 15-3 x 0.0568 + MCA x (-0.33) - 0.53. The sensitivity of the DF in detecting breast cancer was 31% with a specificity of 91% and a efficiency of 52%. The correlation coefficient in breast cancer patients between TAG 12 and CA15-3 measurements was 0.95, and 0.90 between TAG 12 and MCA measurements. In conclusion, the results indicate that TAG 12, CA 15-3 and MCA have only limited value in the diagnosis of breast cancer, but their role in monitoring and follow up of breast cancer patients is a subject for further investigation.

Adult↗
PubMedDate not supplied

Clinical evaluation of a new tumour marker TAG 12 in breast cancer.

The aim of the present study was to evaluate the clinical value of the new tumour marker TAG 12 in sera from patients with breast cancer, patients with benign breast disease and normal controls. The results for TAG 12 were compared with those for CA 15-3 and MCA. The cut-off levels (95th percentile) determined for each test in the benign and control groups were 59 KU/l for TAG 12, 27 KU/l for CA 15-3 and 12 KU/l for MCA. The diagnostic sensitivity of the TAG 12 test was 0.30 (cut-off level 59 KU/l), 0.25 for CA 15-3 test (cut-off level 27 KU/l) and 0.15 for MCA test (cut-off level 12 KU/l) in detecting breast cancer. The combination of TAG 12, CA 15-3 and MCA reached the diagnostic sensitivity 0.35, specificity 0.90, efficiency 0.68, LR+ 3.5, and LR- 0.72 The correlation coefficient in breast cancer patients between TAG 12 and CA 15-3 was 0.99 and the correlation coefficient between TAG 12 and MCA was 0.95. In conclusion, the results indicate that TAG 12, CA 15-3 and MCA have only limited value in the diagnosis of breast cancer, but their role in monitoring and follow-up of breast cancer patients is a subject for further investigation.

Antigens, Neoplasm↗