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Biomedical subjects

L Carlsson

Publications and source records attributed to L Carlsson.

At least 181 records · Page 10Linked to original sources
PubMed1980

Long term stability of lysine vasopressin and of specifically tritiated lysine vasopressin in weakly acidic aqueous solutions.

Lysine vasopressin tritiated at meta position of tyrosine (1.9 Ci/mmole) decomposes during a long storage period, mainly as a result of radiolysis. The loss of specific activity by isotopic exchange with water is slow. The nonlabelled peptide undergoes only very minute decomposition (polymerization). Specific radioactivity declines with a half life of 47.4 months.

Animals↗
PubMed1980

alpha-Actinin promotes polymerization of actin from profilactin.

The effect of alpha-actinin on profilactin has been analyzed. The results show that addition of submolar ratios of alpha-actinin to a profilactin sample leads to a dissociation by the profilin-actin complex and polymerization of actin. The newly formed filaments are cross-linked by alpha-actinin.

Actinin↗
PubMed1979

Reorganization of actin in platelets stimulated by thrombin as measured by the DNase I inhibition assay.

The effect of thrombin stimulation on actin organization in human platelets has been analyzed by using the DNase I inhibition assay, which is selective for unpolymerized and filamentous actin. The results provide biochemical evidence for the suggestion that stimulation leads to rapid polymerization of actin. The measurements also reveal changes in the polymerization state of actin occurring after cell lysis. These changes are influenced by the concentration of free calcium in the extracts.

Actins↗
PubMed1979

The unpolymerised form of actin in non-muscle cells.

Several lines of evidence point to the existence of unpolymerised actin in non-muscle cells. Ultrastructural examination reveals both a variety of actin filament bundles and actin in a controversial organisational state. Arguments are cited that this material, which at least in part is found close to the plasma membrane, represents unpolymerised actin rather than a random array of single actin filaments. The rearrangement of actin filament bundles during the cell cycle, and in response to experimental manipulation, suggests a turnover of filaments by a polymerisation-depolymerisation cycle. Extracts made from non-muscle cells under conditions where muscle actin would polymerise still contain appreciable fractions of monomeric actin. Studies on purified polymerisation-resistant actin from a variety of sources reveal the presence of a small protein which binds specifically to actin and prevents polymerisation. In the last section of the article, we expand the idea that this auxiliary protein is a central control element in the regulated exchange between non-polymerised and polymerised actin in vivo.

Actins↗
PubMed1978

Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.

Actins↗
PubMed1978

Prolonged serum insulin decreasing effects of two synthetic somatostatin analogues studied in vivo by a new animal method. Preliminary communication.

A newly developed in vivo method, using the ob-ob strain of obese-hyperglycaemic mice with permanently very high serum insulin values, makes it possible to detect more prolonged serum insulin lowering properties than in normal animals. Two newly synthesized analogues of somatostatin, D-alanine-somatostatin and des-alanine-des glycine-des-amino-somatostatin produced a more prolonged and greater decrease in the serum insulin values of ob-ob mice than did somatostatin. Our new in vivo method makes it possible to investigate the duration of insulin suppression of new derivatives.

Amino Acid Sequence↗
PubMed1976

Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis.

Thirty sera reacting by IFL technique in titres greater than or equal to 100 with smooth muscle fibres of rat stomach, rat renal glomeruli, and with the membrane region of thyroid cells were randomly chosen among sera sent in for routine testing of tissue antibodies. All sera but one were found to be derived from patients with chronic active hepatitis. The smooth muscle and other relevant cell staining were abolished after absorption of sera with actin, prepared from rabbit skeletal muscle and found to be homogeneous by SDS gel-electrophoresis and by electron microscopy. The actin anti-bodies were purified by precipitation of sera with F-actin and elution of the precipitates at acid pH. The purified antibodies stained all tissues in the same way as the original sera. In double immunodiffusion tests all thirty sera gave precipitation with actin. Thus, it was concluded that these broad-reacting SMA are directed against actin. The finding of high-titred SMA is of diagnostic value and supports the clinical diagnosis of active chronic hepatitis. In addition, anti-actin antibodies eluted from human sera are a suitable tool for studying actin-containing cellular structures.

Actins↗
PubMedDate not supplied

Patient based 'burden-of-illness' in Swedish primary health care. Applying the Johns Hopkins ACG case-mix system in a retrospective study of electronic patient records.

Patients from one municipality in Sweden utilizing primary health care (PHC) during 1998 and 1999 have been categorized into 81 groups. The groups show each patient's own case-mix in terms of illness. Grouping was carried out using the case-mix instrument adjusted clinical groups (ACG), developed by the School of Hygiene and Public Health at Johns Hopkins University in Baltimore, USA. The resulting pattern provided a more adequate reflection of the scope of primary care's task than that yielded by diagnoses alone. Changes over time in terms of illness patterns for a population could be described, analysed and assessed from medical and health economic perspectives. One of the conclusions from this study was that the ACG instrument is a relevant tool in describing the outcome of work by the primary health care centre. The ACG is of interest in the improvement of the quality of primary care in Sweden. The ACG should be a driving force in the development of health indices in both national and international comparisons, as a result of its focus on the health status of patients and populations instead of on diagnoses and diseases.

Acute Disease↗
PubMedDate not supplied

Analysis of the cyanogenic glycoside linamarin in urine by isotachophoresis.

A separation system based on isotachophoresis is described for the cyanogenic glycoside linamarin in aqueous solution and in human urine. Isotachophoresis is a migration of a substance in an electric field, which is applied to a system of electrolytes of specific design. Detection is carried out by monitoring conductivity changes. However, for linamarin in urine, a preseparation procedure was necessary because of the high amount of electrolytes. This was performed by affinity chromatography on a silica sorbent column, with cyclohexyl as the functional group by which linamarin was retained. After elution from the column by methanol, a separation and quantitation of linamarin was possible by means of isotachophoresis. The method allowed determinations of urinary linamarin exceeding 100 microM, with a coefficient of variation of 13% at 500 microM.

Electrophoresis↗