Search PubMed⌕ Search

Biomedical subjects

L Bonomo

Publications and source records attributed to L Bonomo.

At least 361 records · Page 20Linked to original sources
PubMedDate not supplied

Percoll density gradient centrifugation. (An improved method for the enrichment of lymphocyte subsets mediating different activities).

Lymphocyte fractions obtained by Percoll density gradient centrifugation were assayed for their cytotoxic activities both in NK and ADCC cytotoxic systems. Furthermore, the release of a mediator, platelet slowing factor from the same subsets, was evaluated. Different mitogens (phytohemagglutinin, concanavalin A and lipid A) have been used for this purpose. The maximum of cytotoxic activities was recovered in E+ cell-enriched fractions. On the other hand, lipid A was able to stimulate the PSF production from enriched B cells (fraction I); phytohemagglutinin induced the release of PSF from E+ fractions (II and III) and concanavalin A from fraction II only. Our results suggest that Percoll density gradient is a very reproducible method in obtaining lymphocyte subsets enriched in cells mediating either cytotoxic activities or lymphokine release.

Antibody-Dependent Cell Cytotoxicity↗
PubMedDate not supplied

Natural and antibody-dependent cellular cytotoxicity in patients with active sarcoidosis.

Lymphocyte surface markers, natural killer activity, antibody-dependent cellular cytotoxicity and lymphocyte binding to target cells were evaluated in a group of 16 subjects with active sarcoidosis. A reduction of E-RFC and a relative increase of EA-RFC were observed. At the same time, ADCC activity was similar to that of normal controls, while NK cytotoxicity was significantly decreased. On the other hand, the percentage of conjugates between lymphocytes and target cells was lower than in controls. These observations account for a selective defect of NK activity, suggesting that the NK assay might be an useful tool to support the impairment of cellular immunity in active sarcoidosis.

Adult↗
PubMedDate not supplied

[Thoracic radiography. Comparison of asymmetric screen-film, technic of mechanical homogenization, and conventional technics].

This study was aimed at comparing three different systems, i.e., asymmetric screen-film, mechanical homogenized and conventional techniques, in standing frontal and lateral plain radiographs of the chest. Two hundred consecutive patients with normal posteroanterior (PA) and lateral films were randomly subdivided into 4 groups. Each group was submitted to chest radiographs with a different technique: asymmetric screen-film systems (InSight HC and High Light GCA), mechanical homogenized (Tau-Gil Homogenized) and conventional high-kilovoltage techniques. The exposure values for frontal projections ranged 110 kV (InSight HC) to 141 kV (Tau-Gil), while for lateral projections they ranged 123 kV (conventional technique) to 143 kV (Tau-Gil). Statistically significant differences were observed between the two asymmetric systems as regards exposure values, High Light exhibiting higher mean values in the frontal projection (t-test p < 0.05). Image quality was studied jointly by 3 experienced chest radiologists. The observers were asked to grade, on a 3-point ordinal scale, the conspicuity of mediastinal borders, of pulmonary vessels and of selected areas of lung parenchyma (i.e., retrocardiac, retrosternal and apical regions), as well as overall image quality on the frontal projection. The statistical analysis of paired differences was performed with the Mann-Whitney U-test. The asymmetric and the mechanical homogenized techniques were much better than the conventional technique in depicting tracheobronchial tree, retrocardiac parenchyma, azygos-esophageal recess and thoracic spine (p < 0.05). The mechanical homogenized system provided best overall image quality on frontal films, being superior to both InSight HC and conventional techniques, but not to the High Light GCA system; only the frontal projection obtained with the homogenized technique was compared, no filter being available for the lateral projection. When the two asymmetric systems were compared, the High Light system better depicted vascular structures on frontal films (p < 0.05), while apical areas were better demonstrated with the InSight system, namely with lateral films (p < 0.05).

Female↗