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Biomedical subjects

L Andersson

Publications and source records attributed to L Andersson.

At least 451 records · Page 25Linked to original sources
PubMed1980

External irradiation therapy in carcinoma of the prostate.

107 patients with poorly differentiated prostatic carcinoma without metastases received local irradiation at the Karolinska Hospital from 1965 through 1973. The majority of the patients had received previous hormone therapy and had a progressive local disease. Many of them were given estrogens even after radiotherapy. All the patients were followed for at least 5 years. Complete regression of the palpable prostatic carcinoma mass was registered in about 70% of the cases. A 33% 5-year survival was obtained. This is significantly higher than the survival of the comparable group of patients receiving only hormones.

Aged↗
PubMed1980

Diagnosis and categorization of the primary tumour in carcinoma of the prostate.

Histologic or cytologic verification of the diagnosis is mandatory. In the majority of cases the tumour originates in the peripheral portion of the gland. It is often multifocal. Perineal puncture biopsy and transrectal aspiration biopsy are of equal value to verify the diagnosis and establish the malignancy grading. Palpation is rather accurate to detect extraprostatic tumour growth but not to evaluate the tumour extension within the gland or the occurrence of multifocal tumour. Of the modern imaging techniques transrectal ultrasound scanning appears superior to visualize small or multifocal tumours.

Humans↗
PubMed1979

A 1,4-beta-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414. Purification, characterization and preparation of an immunoadsorbent for the enzyme.

A 1,4-beta-glucan glucanohydrolase (EC 3.2.1.4) was isolated from culture filtrates of the fungus Trichoderma viride QM 9414 by molecular-sieve chromatography on Bio-Gel P-30, ion-exchange chromatography on DEAE-Sephadex A-50 and isoelectric focusing in a density gradient. Polyacrylamide-gel electrophoresis at two different pH values, analytical isoelectric focusing in a polyacrylamide-gel slab and molecular-sieve chromatography of the reduced and alkylated enzyme in a denaturing medium indicated a homogeneous protein. The enzyme has a mol.wt. of 51,000 and is not a glycoprotein. The pI was found to be 4.66 at 23 degrees C. Antiserum against the purified enzyme was prepared and the amount of enzyme in the original filtrate was determined by rocket immunoelectrophoresis to be about 50mg/liter. An immunoadsorbent made from CNBr-activated sepharose 4B and antiserum affords a rapid and highly specific purification of the enzyme.

Amino Acids↗
PubMed1979

The use of biochemical solid-phase techniques in the study of alcohol dehydrogenase. 1. Some studies on subunit interactions in alcohol dehydrogenase with subunits covalently bound to agarose.

The EE and SS isozymes of horse liver alcohol dehydrogenase have been immobilized separately to weakly CNBr-activated Sepharose 4B. The resulting immobilized dimeric preparations lost practically all of their activity after treatment with 6 M urea. However, enzyme activity was regenerated by allowing the urea-treated Sepharose-bound alcohol dehydrogenase to interact specifically with either soluble subunits of dissociated horse liver alcohol dehydrogenase or soluble dimeric enzyme. The regeneration of steroid activity in the immobilized preparations after treatment of the bound S subunits with soluble E subunits seems to show that true reassociation of the enzyme had taken place on the solid phase, since only isozymes with an S-polypeptide chain are active when using 5 beta-dihydrotestosterone as substrate. The results presented in this paper indicate that immobilized single subunits of horse liver alcohol dehydrogenase are inactive and that dimer formation is a prerequisite for the enzymic activity.

Alcohol Oxidoreductases↗
PubMed1978

Purification and characterization of a low molecular weight 1,4-beta-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414.

A low molecular weight 1,4-beta-glucan glucanohydrolase (endoglucanase) (1,4-(1,3;1,4)-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.4) has been isolated from culture filtrates of the fungus Trichoderma viride QM 9414 by a two-step procedure of gel filtration and ion-exchange chromatography. The isolated enzyme appeared homogeneous upon polyacrylamide gel electrophoresis at pH 2.9, isoelectric focusing in a polyacrylamide gel slab, sedimentation equilibrium analysis and chromatography of the reduced and alkylated enzyme on a column of Sepharose 6B in 6 M guanidine - HCl. A molecular weight was calculated at approx. 20 000 and the isoelectric point was determined at pH 7.52. The purified enzyme was not a carbohydrate-containing protein.

Amino Acids↗
PubMed1978

Special considerations with regard to the dosage of tranexamic acid in patients with chronic renal diseases.

Tranexamic acid is a potent antifibrinolytic drug frequently used in the treatment of haematuria and a number of other haemorrhagic conditions. Since it is eliminated mainly in the urine, the drug accumulates in patients with uraemia. The excretion of tranexamic acid in patients with renal failure has been investigated and dosage recommendations are given for tranexamic acid therapy in cases of renal failure.

Biological Availability↗
PubMed1978

Chemotherapy in bladder carcinoma.

In a series of patients with recurrent bladder carcinoma, treatment with various cytostatic drugs was generally unsatisfactory in cases of infiltrating carcinoma. Better results were achieved using instillation of Thiotepa for bladder papillomatosis and by instillation of Adriamycin for carcinoma in situ.

Antineoplastic Agents↗