Biomedical subjects

Ke Yao

Publications and source records attributed to Ke Yao.

60 records · Page 4Linked to original sources

PubMed2002

Treatment of corneal epithelial in-growth after excimer laser in situ keratomileusis.

OBJECTIVE: To evaluate efficacy of photo therapeutic keratectomy (PTK) on corneal epithelial growth under the lamellar flap after excimer laser in situ keratomileusis (LASIK). METHODS: In the 6 eyes with epithelial in-growth, the corneal cap was lifted, and the in-growing epithelium was removed from the interface. Five to 10 pulses of PTK being 7 mm in diameter were placed over the undersurface of the flap and the stromal bed, irrigation between the interface and replacing corneal flap were performed, and 50 to 75 pulses PTK being 3 mm in diameter were utilized to smooth the flap edge. The follow-up period ranged from 5 to 12 months. RESULTS: In the follow-up, no epithelial implantation reoccurred in these 6 eyes. The uncorrected visual acuity achieved their best preoperative corrected visual acuity. CONCLUSION: The results indicate that PTK can prevent recurrent epithelial in-growth after LASIK.

Cornea↗

PubMed2002

[Influence of low molecular weight heparin on proliferation of bovine lens epithelial cells on the surface of intraocular lens].

OBJECTIVE: To investigate the anti-proliferation effect of low molecular weight heparin (LMWH) on bovine lens epithelial cells (BLEC) on the surface of polymethylmethacrylate (PMMA) intraocular lens (IOL). METHODS: BLECs were incubated in vitro on the surface of PMMA IOLs. The cultural media contained LMWH (1 x 10(5) U/L) or normal heparin (1 x 10(5) U/L) or none of them (control). Cells were counted by flow cytometry. RESULTS: The heparin or LMWH treated BLECs on the surface of PMMA IOLs showed immature appearance, while the BLECs in the control group showed a tendency of fibrosis. The numbers of BLECs (per ml) in the digested solution from the surface of IOLs were 878.2 +/- 107.8 (control), 545.5 +/- 58.2 (heparin) and 407.8 +/- 36.6 (LMWH). The comparisons among the three groups showed significant differences (P < 0.001). CONCLUSION: Low molecular weight heparin inhibits proliferation of lens epithelial cells on the surface of intraocular lens, which could be used as a potential drug to prevent the occurrence of postoperative capsular opacification after IOL implantation.

Animals↗

PubMed2002

Changes in gap junctional intercellular communication in rabbits lens epithelial cells induced by low power density microwave radiation.

OBJECTIVE: To demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms. METHODS: Rabbits' eyes were exposed to 5 mW/cm(2) and 10 mW/cm(2) power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy. RESULTS: The GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm(2) irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm(2) x 3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm(2) group. CONCLUSIONS: Low power densities microwave radiation (5 mW/cm(2) and 10 mW/cm(2)) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm(2) or 10 mW/cm(2) microwave radiation is cataractogenic.

Animals↗

PubMed2002

[Frequency-doubling perimetry in examination of open-angle glaucoma with hemifield defect].

OBJECTIVE: To determine whether frequency-doubling perimetry (FDP) can find visual field defect of early glaucoma which can not be found by conventional perimetry. METHODS: Forty-nine eyes of 49 patients with open-angle glaucoma and hemifield defect detected by Humphery Field Analyzer (HFA) were tested by both FDP (threshold test C-20) and Heidelberg Retina Tomograph (HRT). For each hemifield, its FDP result was evaluated. According to the FDP results in intact HFA hemifields, the patients were divided into FDP normal group and FDP abnormal group. The HRT parameters of half disk corresponding to the intact HFA hemifields were compared between these two groups. RESULTS: There was a strong correlation between the mean sensitivity of FDP and mean deviation of HFA results (r = 0.58, P < 0.01). In intact HFA hemifields, 41% (20/49) of eyes showed visual loss in FDP tests, while in defective HFA hemifields, only 2% (1/49) of eyes was normal in FDP test. For the HRT parameters of half disk corresponding to the intact HFA hemifields, cup area and cup/disk area ratio were statistically bigger and rim area and rim volume were statistically smaller in FDP abnormal group than in FDP normal group. CONCLUSION: FDP is able to detect early glaucomatous visual field damage which can not be detected by HFA, but can be explained by the optic nerve glaucomatous changes in HRT parameters.

Adult↗

PubMedDate not supplied

Bone marrow-derived cells are present in Mooren's ulcer.

To investigate whether bone marrow-derived cells are present in Mooren's ulcer and involved in its destructive and regenerative disease course, tissue specimens were collected from 3 eyes of 3 patients with Mooren's ulcer that underwent lamellar keratectomy. Three normal donor limbal corneoscleras served as controls. Immunohistochemical staining patterns were analyzed by using the following antibodies: CD34 (a marker of hematopoietic progenitor cells and endothelium), c-kit (a marker of hematopoietic and stromal progenitor cells) and STRO-1 (a differentiation antigen present on bone marrow fibroblast cells and on various nonhematopoietic progenitor cells). Strong positive CD34, c-kit and STRO-1 cells were revealed in Mooren's ulcer specimens, especially in the superficial stroma. A few weakly expressed CD34 stromal cells were seen in normal limbal cornea, but no immunoreactivity for c-kit and STRO-1 was found. Bone marrow-derived cells are present in Mooren's ulcer and contribute to its destructive and regeneration process by synergizing with other factors. Specific therapeutic strategies that target the role of these cells in Mooren's ulcer are anticipated.

Antigens, CD34↗

PubMedDate not supplied

Mitochondrial DNA 4977 BP deletion mutations in lung carcinoma.

BACKGROUND: The most common and also the most often assayed mtDNA deletion mutation, degrees mtDNA 4,977 sub has been demonstrated in various types of human cancer. However, knowledge about degrees mtDNA 4,977 in lung carcinoma is poor. AIM: To study the 4,977 bp deletions of mitochondrial DNA ( degrees mtDNA 4,977) in lung cancer, adjacent histologically normal and normal lung tissue and its potential roles in the development of cancer. MATERIALS AND METHODS: Thirty-seven matched lung cancer/adjacent histologically normal and 20 histologically normal lung tissue samples in subjects without lung cancer were analyzed by PCR technique. RESULTS: degrees mtDNA 4,977 deletions were detected in 54.1% (20/37) of lung cancers, 59.5% (22/37) of adjacent normal and 30.0% (6/20) of normal lung tissue samples. No significant difference was found in the frequency of degrees mtDNA 4,977 deletions between the tumor and adjacent normal lung tissues (P value = 0.815). Moreover, no significant difference was found in the frequency of degrees mtDNA 4,977 deletions between the tumor and histologically normal lung tissues in subjects without lung cancer (P value=0.101). However, the correlation between degrees mtDNA 4,977 deletion and age and smoking factors was present in our data. STATISTICAL ANALYSIS: Fisher's exact test was used to assess the difference in different groups by the Scientific Package for Social Sciences (SPSS), version 10.0, Statistical analysis software. CONCLUSIONS: Mitochondrial DNA 4,977 bp deletion, which is not specific to lung cancer, may reflect the environmental and aging process influences operative during tumor progression.

Adenocarcinoma↗