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Biomedical subjects

K Yazaki

Publications and source records attributed to K Yazaki.

At least 91 records · Page 5Linked to original sources
PubMed1989

[Ruptured nonparasitic liver cyst caused by blunt abdominal trauma].

Three patients with ruptured nonparasitic liver cyst (two with suspected multiple liver cysts and one with polycystic disease) following blunt abdominal trauma were described. Ultrasonographic examinations and computed tomography (CT) scans were useful in evaluating the presence and degree of liver injury, the amount of hemoperitoneum and the need for operative repair. Two patients with intraperitoneal bleeding were treated by transcatheter hepatic arterial embolization and all three patients were successfully managed nonoperatively.

Abdominal Injuries↗
PubMed1987

Unique variants of hCG in sera of choriocarcinoma patients.

Human chorionic gonadotropin (hCG) of pregnancy can be separated into several variants by an isoelectric focusing (IEF) method. The hCG produced by choriocarcinoma consists of components found in pregnancy sera (pI 3.9-6.0) and, in addition, unique variants with more acidic isoelectric points ranging from 3.2 to 3.7. The sera of all five patients with advanced choriocarcinoma contained these unique acidic variants of hCG and the immunoreactive levels were significantly higher in these patients than in normal pregnant women or patients afflicted with hydatidiform mole or invasive mole. When the acidic variants of hCG were treated with neuraminidase they migrated to the alkaline region (pI 9-11) of the IEF column. In conclusion, sera of choriocarcinoma patients contain unique variants of hCG. The finding that these highly acidic components can be converted to molecules with an alkaline pI by neuraminidase digestion suggests that this high negative charge may be due to an increased sialic acid content. Patients possessing the acidic variants of hCG in their sera had poor survival rate. Determination of the variants may be useful as a prognosis marker and in evaluating the efficacy of therapy.

Chorionic Gonadotropin↗
PubMed1986

A new method for extracting circular and supercoiled genome segments from cytoplasmic polyhedrosis virus.

The genome of a cytoplasmic polyhedrosis virus (CPV) consisting of 10 segments was extracted by a new two-step extraction method. The results from polyacrylamide gel electrophoresis, phenol and diepoxybutane treatment and electron microscopic examination indicated the extracted genome segments to have circular and supercoiled structures as genome-protein(s) complexes. Multiple cycles of the transcription of each segmented genome of CPV may take place on the circular structure of the segment.

Animals↗
PubMed1986

Multifocal ventricular ectopic complexes possibly related to diuretic induced hypokalemia in a woman with the long QT syndrome.

A 40-year-old housewife was observed with chief complaints of syncope and palpitation. The electrocardiogram indicated premature ventricular ectopic complexes of the torsade de pointes. Serum potassium level was low because of thiazide-induced hypokalemia for the treatment of transient hypertension. Even after the disappearance of threatening ventricular arrhythmias and normalization of serum potassium level, prolongation of QT interval was continuously persisted. Thiazide induced hypokalemia might have some roles on producing ventricular ectopic activity in prolonged QT syndrome as shown in our case.

Adult↗
PubMed1985

Biological activity of the unique variants of human chorionic gonadotropin in choriocarcinoma tissue.

Human chorionic gonadotropin (hCG) in sera and placentae from normal pregnant women separated into 7 variants on analysis by an isoelectric focusing technique and determined by radioimmunoassay. The pIs ranged from 3.9 to 7.0. Three additional acidic variants were found in the sera and tumor tissues of patients with choriocarcinoma with pIs of 3.2, 3.5 and 3.7. The biological activity of each variant was determined by measuring testosterone production by rat Leydig cells in vitro. The pI 4.1 fraction corresponding to placental hCG possessed the highest biological activity while those focusing further afield from pI 4.1 showed decreasing activities. All 3 tumor unique acidic variants possessed biological activity with the fraction focusing at pI 3.7 having the greatest potency.

Biological Assay↗
PubMed1983

Demonstration of stepwise coiling of nucleoprotein in adenovirus core by application of critical point drying.

Mild destruction of a virus particle to observe the organized structure of the nucleoprotein complex in a virion was achieved by application of the critical point drying method. Adenovirus type 12 (ad12) virions have been treated by this method after the particles had been fixed with glutaraldehyde on an electron microscope grid. With 15 min prefixation, the capsids (shells) and the cores were in various stages of unfolding. The core was unfolded in the filamentous structure. The thickness of these filaments was 6.7, 13.3, +23 nm, or more. Some pictures showed that the thicker filaments consisted of super-coiling of two thinner filaments, for example two 6.7-nm filaments coiled up to give the 13.3-nm filaments. This suggests that the nucleoprotein complex of a circular double-stranded DNA and inner proteins of ad12 virus was folded in a stepwise fashion to produce the compacted form of the core.

Adenoviruses, Human↗
PubMed1982

Dependence of tumor-forming capacities of cells transformed by recombinants between adenovirus types 5 and 12 on expression of early region 1.

Recombinants between an adenovirus type 5 (Ad5) deletion mutant and the Ad12 DNA fragment containing early region 1 (E1) were isolated from cells cotransfected with the EcoRI-C fragment of Ad12 DNA and Ad5 dl312 (deletion in E1A) DNA (rcA) and from cells cotransfected with the SalI-C fragment of Ad12 DNA and Ad5 dl312 DNA (rcB). No recombinant was isolated from cells cotransfected with Ad5 dl313 (deletion in E1B) DNA and restriction fragments of Ad12 DNA. Both rcA and rcB are defective and able to replicate in human embryo kidney (HEK) and KB cells with complementation by dl312. Both rcA and rcB formed Ad12 T antigen g, but not T antigen f, in infected HEK and KB cells. In rcA- and rcB-infected cells, Ad5 E1B and Ad12 E1A genes are transcribed. Heteroduplex and size analyses of rcA-1 or rcB-1 DNA fragments hybridized with Ad12 DNA revealed that rcA-1 DNA has a deletion between 5 and 15 map units with an insertion of a portion of Ad12 DNA (10%) and that rcB-1 DNA has a deletion between 70 and 80 map units with an insertion of a portion of Ad12 DNA (10%). The transformed cell lines, RCAY and RCBY, were established after infection of rat 3Y1 cells with rcA and rcB, respectively. Both Ad5 and Ad12 DNA sequences are contained in these cells. In RCAY cells, Ad12 T antigen g is detected, but Ad12 T antigen f is not. In RCBY cells, both Ad12 T antigen g and f are detected. Only the Ad12 E1A gene is transcribed in RCAY cells, whereas Ad5 E1B, Ad12 E1A, and Ad12 E1B genes are transcribed in RCBY cells. In soft-agar cultures, RCBY cells form large colonies, whereas RCAY cells form only tiny colonies. RCBY cells form tumors as efficiently as 12WY cells in transplanted rats. RCAY cells formed tumors inefficiently. Ad5-transformed 5WY cells do not form tumors. These observations indicate that the efficient tumor formation by RCBY cells is dependent on the expression of the Ad12 E1A and E1B genes, whereas the inefficient tumor formation by RCAY cells is due to the expression of only the Ad12 E1A gene.

Adenoviruses, Human↗
PubMed1982

Hypokalemic myopathy associated with 17 alpha-hydroxylase deficiency: a case report.

We studied a patient with hypokalemic myopathy associated with 17 alpha-hydroxylase deficiency. An 18-year-old high school student, who appeared to be a girl with poorly developed secondary sex characteristics, had generalized muscle weakness. The cause of muscle weakness proved to be hypokalemic myopathy confirmed by clinical findings and muscle biopsy. Endocrinologic study demonstrated 17 alpha-hydroxylase deficiency with male pseudohermaphroditism. The metabolic abnormality of this patient was corrected by the administration of glucocorticoid. The possibility of this rare disease has to be considered when we examine a patient who has hypokalemic myopathy associated with hypogonadism.

Adolescent↗