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Biomedical subjects

K Yanagi

Publications and source records attributed to K Yanagi.

At least 127 records · Page 7Linked to original sources
PubMed1991

Goblet cell carcinoid of the vermiform appendix with ovarian metastasis mimicking mucinous cystadenocarcinoma.

We describe a case of goblet cell carcinoid of the vermiform appendix, which metastasized to the bilateral ovaries, uterus, vagina and peritoneum three years and four months after appendectomy. The appendiceal tumor showed transmural infiltration of carcinoembryonic antigen-positive goblet cell-type tumor cells, intermingled with a small number of argyrophilic cells immunoreactive for serotonin and chromogranin A. The presence of neurosecretory-type granules was confirmed ultrastructurally in some mucin-negative tumor cells. The pattern of proliferation was typical of carcinoid tumor originating from the lower-most part of the mucosa, and showed lymphatic permeation. The metastatic lesions in the pelvic organs showed either diffuse infiltration of goblet cell-type tumor cells or mucinous cystadenocarcinoma-like features, the latter being evident in the right ovary. No neuroendocrine component was identified in the metastatic deposits. The kinship of appendiceal goblet cell carcinoid to adenocarcinoma is discussed.

Adult↗
PubMed1991

Extracellular production of mouse interferon beta by the Bacillus subtilis alpha-amylase secretion vectors: antiviral activity and deduced NH2-terminal amino acid sequences of the secreted proteins.

Using two Bacillus subtilis alpha-amylase secretion vectors, mouse interferon-beta (IFN-beta) cDNA was expressed. Ten kinds of mature and hybrid forms of biologically active mouse IFN-beta proteins were synthesized in B. subtilis and were secreted into the culture medium. Similar amounts of proteins, which were determined by immunoblot analysis, were secreted from the B. subtilis transformants, while the antiviral activity in the medium was markedly different in each one of them. The highest antiviral activity was observed in a hybrid protein, in which eleven amino acids consisting of the prosequence of alpha-amylase (eight amino acids) and three amino acids encoding in the HindIII linker DNA were assumed to be extended in front of the Leu residue at position 6 of the mature form of mouse IFN-beta. Its activity was higher than that of the mature form of the mouse IFN-beta secreted from a B. subtilis transformant. These results indicate that the amino acid sequence around the NH2-terminal region of the mouse IFN-beta is closely related to the antiviral activity, but the NH2-terminal amino acid is not. The hybrid proteins of mouse IFN-beta show no interferon activity against a human cell line, HEL.

Amino Acid Sequence↗
PubMed1991

[A case of coronary artery bypass utilizing natural bifurcation of the right internal mammary artery].

A Forty five year old male with unstable angina due to multiple coronary artery stenosis underwent successful complete coronary revascularization utilizing the natural bifurcation of the right internal mammary artery. As this procedure has not been reported in detail previously in the literature and since the use of the natural bifurcation of the internal mammary artery was considered to be useful for the treatment of multiple coronary stenosis, this case was presented. Pre and post operative angiography is included.

Angina, Unstable↗
PubMed1990

Biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins.

1. Perchloric acid-soluble glycoprotein fractions (PASFs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (OCAs) in benign and borderline and ovarian clear cell carcinoma (OCC) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. These PASFs reacted with anti-A, -B, -Lea and/or -Leb sera, but did not react with anti-H, -M and -N sera. All of the PASFs reacted with Ricinus communis lectin (RCA-I). Among these PASFs, PASFs from the cyst fluids of OCA in benign did not react with Arachis hypogaea anti-T lectin (PNA) and both of the anti-N lectins of Vicia graminea (VGA) and Vicia unijuga (VUA), and PASFs from OCA in borderline reacted with only PNA and PASFs from OCC in malignant reacted with the three lectins, PNA, VGA and VUA. However, none of PASFs from human normal sera and various normal organ tissues reacted with any of RCA, PNA, VGA and VUA. 3. The above-mentioned results seem to show that the examination of glycoproteins of ovarian cyst fluids by the combined use of RCA-I, PNA and VGA (or VUA) permits biochemical diagnosis of the canceration degree of ovarian cystomas.

Adenocarcinoma↗
PubMed1990

Purification and characterization of anti-N lectin from Vicia unijuga leaves.

1. An anti-N lectin was extracted from Vicia unijuga leaves with phosphate-buffered saline (PBS). Purification of the lectin was achieved, after pretreatment of the PBS extract by ammonium sulfate fractionation and absorption with human M erythrocytes, by using a combination of conventional chromatographic techniques with asialoglycophorin AN-Sepharose CL-4B affinity chromatography. Purification steps were followed by increase of specific activity. 2. Homogeneity of the purified lectin was demonstrated by HPLC and SDS-PAGE. The purified lectin was a glycoprotein with 11.4% carbohydrate and relatively high percentages of serine, threonine and aspartic acid residues and had a Mw of 120,000 Da. 3. This lectin agglutinated human N and MN erythrocytes, but did not agglutinate M erythrocytes. Hemagglutination of the lectin was inhibited by glycophorin AN and N-active sialoglycopeptide released from human N erythrocytes by treatment with Pronase or trypsin. However, it was not inhibited by any of mono- and di-saccharides, ABH-active glycoproteins, glycophorin AM and M-active sialoglycopeptide liberated from human M erythrocytes by treatment with Pronase or trypsin.

Amino Acids↗
PubMed1990

Isolation and characterization of blood group N antigen precursor glycoproteins with Thomsen-Friedenreich (T) activity, N antigen precursor glycoproteins and T-active glycoproteins from cyst fluids of malignant ovarian clear cell carcinoma.

1. Two perchloric acid-soluble glycoprotein fractions from cyst fluids of 2 patients with ovarian clear cell carcinoma in malignant were subjected to a systematic affinity chromatography using Vicia unijuga lectin-Cellulofine column and Arachis hypogaea lectin-Cellulofine column and separated into four glycoproteins, blood group N antigen precursor glycoprotein with Thomsen-Friedenreich (T) activity, N antigen precursor glycoprotein, T-active glycoprotein and serologically inactive glycoprotein, respectively. 2. These serologically active glycoproteins isolated in yields of 0.3-3.3% of PASFs, were proved to be mucin-type glycoproteins with Mw of 509,000-2,772,000 Da and contained 33.9-81.7% carbohydrates.

Acetylgalactosamine↗
PubMed1990

Separation and biochemical characterization of blood group N antigen precursor glycoproteins with Thomsen-Friedenreich (T) activity, T-active glycoproteins and N antigen precursor glycoproteins from ascites of primary ovarian cancer patients.

1. Three perchloric acid-soluble fractions from ascites of three primary ovarian cancer patients were subjected to Sephacryl S-300 gel filtration, respectively, and three Fr. 1 which were eluted in the vicinity of void volume as minor fractions, were then separated by a systematic affinity chromatography using Vicia unijuga lectin-Sepharose CL-4B column and Arachis hypogaea lectin-Sepharose CL-4B column into three glycoproteins, blood group N antigen precursor glycoprotein with Thomsen-Friedenreich (T) activity, T-active glycoprotein and N antigen precursor glycoprotein, respectively. 2. These nine glycoproteins separated in yields of 0.1-1.3 mg per 100 ml of ascites, were demonstrated to be mucin-type glycoproteins with Mw of 1,791,000-4,921,000 and contained 33.8-56.1% carbohydrates.

Amino Acids↗
PubMed1990

[Epidemiologic analysis of DNA's of herpes simplex virus, type 1 and type 2 isolates obtained from family members and from different lesions of individuals].

We analyzed herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2) infections in 6 families, to see whether or not a single strain had infected members of each family, analyzing DNA's of viral isolates with restriction endonucleases. We also analyzed 29 isolates from 13 patients to examine if herpetic lesions on different sites of each patient's body had been caused by a single strain. In 4 cases of family infection and 10 cases of individual infection with multiple site manifestations, electrophoretic profiles of digested DNAs of the epidemiologically closely related HSV-1 or HSV-2 isolates were exactly the same. In the other 2 family infections and the other 3 individual infections, there were some differences in size of certain DNA fragments among the isolates which were epidemiologically related to one another. But, these differences did not necessarily indicate that they were distinct strains. Thus, it has been suggested that a single strain spreads among members of a family, and herpetic lesions on different sites of an individual body are caused by the same viral strain in most cases of HSV-1 or HSV-2 infections.

Adult↗
PubMed1990

[Separation of Thomsen-Friedenreich (T) antigen and N antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties].

One M perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group AB) with ovarian clear cell carcinoma in malignant was subjected to Sephacryl S-200 gel filtration and Fraction 1 which was eluted at void volume, reacted with both of the lectins of Vicia unijuga and Arachis hypogaea. This fraction was then separated into 6 fractions (DEAE-Frs. 1-6) in a DEAE-Sephacel column chromatography. DEAE-Frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and N-acetylglucosamine in remarkably high percentages, respectively. Reactivities against various hemagglutinins of these four fractions suggest that DEAE-Fr. 3 is a Thomsen-Friedenreich (T) glycoprotein fraction with AB activity and weak N antigen precursor activity and DEAE-Fr. 4 is an N antigen precursor glycoprotein fraction with AB activity and weak T activity.

Adenocarcinoma↗
PubMed1989

Destabilization of herpes simplex virus type 1 virions by local anesthetics, alkaline pH, and calcium depletion.

The infectivity of herpes simplex virus type 1 (HSV-1) was found to be markedly reduced by treating virions with the tertiary amine local anesthetics lidocaine, dibucaine and tetracaine. These treatments induced a characteristic shift in the buoyant density of the HSV-1 particles from a "light" to a "heavy" population. HSV-1 virions were unstable at alkaline pH, and alkali treatment caused the same shift in buoyant density. Ca2+ stabilized the "light" population. These results suggest that the physicochemical status of the HSV-1 envelope which is sensitive to the treatments described above plays an important role for the integrity of the virion.

Anesthetics, Local↗
PubMed1989

[Localization of a variant of herpes simplex virus type 1 in the western Japan].

We previously reported that a variant of herpes simplex virus type 1, which we found by Bg1 II digestion of many Japanese HSV-1 isolates' DNA's, was isolated frequently in two regions of Western Japan, i.e. Yamaguchi and Fukuoka. In this paper, we analyzed 280 more isolates from eleven regions in Japan. In four western regions of Japan, i.e. Osaka, Shimane, Yamaguchi, and Fukuoka, the isolation frequency of the variant was very high, and its proportion among all the isolates from those regions was 30 to 50%. On the other hand, in four eastern regions of Japan, i.e. Akita, Iwate, Miyagi, and Yamanashi, the proportion was 0 to 11%. In two central regions, i.e. Aichi and Kyoto, the proportions were 16 and 22%, respectively. Thus, the variant seems to be localized in the western regions of Japan.

DNA, Viral↗
PubMed1989

[Some chemical and serological properties of non-dialysable fractions of human seminal plasmas].

Some chemical and serological properties of water-soluble and undialysable fraction (WSF) and water-insoluble and undialysable fraction (WISF) of human seminal plasmas were studied. Both of the fractions contained proteins as the main components and also carbohydrates as minor components, and each fraction gave 9 bands which were stained with the Periodate-Schiff and/or Coomassie brilliant blue reagents on sodium dodecyl sulfate-polyacrylamide slub gel electrophoresis. WSF reacted with 17 of 19 hemagglutinins, whereas WISF reacted with 12 of them. Although WSF and WISF did not react with anti-M and -N sera respectively, both of the fractions reacted with the anti-N lectins of Vicia graminea and V. unijuga and also with Arachis hypogaea anti-T (Thomsen-Friedenreich) lectin. Perchloric acid-soluble fractions separated from a large number of human intact organ tissues and serum did not react with the above-mentioned 5 hemagglutinins. In double immuno-diffusion, both of the fractions interacted with rabbit anti-WSF serum to form 4 precipitation lines.

Antigen-Antibody Reactions↗