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Biomedical subjects

K Yamane

Publications and source records attributed to K Yamane.

At least 307 records · Page 17Linked to original sources
PubMed1985

Beneficial effect of topical fibronectin in patients with keratoconjunctivitis sicca of Sjögren's syndrome.

Fibronectin, a high molecular weight plasma protein, was applied locally to 12 patients with keratoconjunctivitis sicca of Sjögren's syndrome refractory to conventional treatment. All patients had ophthalmologically proved keratoconjunctivitis sicca of unknown etiology and were suffering from severe eye symptoms. Fibronectin was purified from autologous plasma and administered to the patients as tear drops 3 times a day. All patients responded to the therapy with fewer subjective symptoms and decreased fluorescein staining. All but 3 responded to the therapy as judged by rose bengal test. No adverse effects were encountered. We conclude that local application of fibronectin may benefit patients with keratoconjunctivitis sicca of Sjögren's syndrome.

Fibronectins↗
PubMed1984

Length and structural effect of signal peptides derived from Bacillus subtilis alpha-amylase on secretion of Escherichia coli beta-lactamase in B. subtilis cells.

The precursor of Bacillus subtilis alpha-amylase contains an NH2-terminal extension of 41 amino acid residues as the signal sequence. The E. coli beta-lactamase structural gene was fused with the DNA for the promoter and signal sequence regions. Activity of beta-lactamase was expressed and more than 95% of the activity was secreted into the culture medium. DNA fragments coding for short signal sequences 28, 31, and 33 amino acids from the initiator Met were prepared and fused with the beta-lactamase structural gene. The sequences of 31 and 33 amino acid residues with Ala COOH-terminal amino acid were able to secrete active beta-lactamase from B. subtilis cells. However beta-lactamase was not secreted into the culture medium by the shorter signal sequence of 28 amino acid residues, which was not cleaved. Molecular weight analysis of the extracellular and cell-bound beta-lactamase suggested that the signal peptide of B. subtilis alpha-amylase was the first 31 amino acids from the initiator Met. The significance of these results was discussed in relation to the predicted secondary structure of the signal sequences.

Amino Acid Sequence↗
PubMed1984

Isolation of a novel antibody, which precipitates ribonucleoprotein complex containing threonine tRNA from a patient with polymyositis.

We have isolated and characterized a novel antibody from a patient with polymyositis, which precipitates threonine tRNA and an unknown small RNA molecule from 32P-labeled HeLa extract. Fingerprint analysis showed that the human threonine tRNA precipitated was nearly identical to the murine tRNA described by Harada [(1978) Seikagaku 50, 397-411]. Nucleotide analysis suggested that the other small RNA molecule might be transfer RNA. Since phenol extraction resulted in the loss of immunoprecipitability, the protein portion was presumed to be involved in the recognition of the antigen. Immunofluorescence staining of HeLa cells with the antibody clearly demonstrated that the antigen was located predominantly in the cytoplasm.

Autoantibodies↗
PubMed1984

A Bacillus subtilis secretion vector system derived from the B. subtilis alpha-amylase promoter and signal sequence region, and secretion of Escherichia coli beta-lactamase by the vector system.

A secretion vector system in Bacillus subtilis was constructed from the alpha-amylase promoter and signal sequence coding region of an alpha-amylase hyperproducing strain, B. subtilis NA64, and the major part of the plasmid pTUB4 which was derived from pUB110. When an Escherichia coli beta-lactamase gene, lacking its own promoter and signal sequence coding region, was introduced into the secretion vector system, beta-lactamase was expressed in B. subtilis. In addition, more than 95% of the enzyme synthesized was secreted into the culture medium via the secretion vector system. Secreted beta-lactamase crossreacted with rabbit antiserum raised against the E. coli enzyme.

Bacillus subtilis↗
PubMed1984

Changes in the properties and molecular weights of Bacillus subtilis M-type and N-type alpha-amylases resulting from a spontaneous deletion.

The regulatory gene, amyR2, and the structural gene, amyEn+, coding for N-type alpha-amylase from Bacillus subtilis N7 have been cloned in the B. subtilis plasmid pUB110. The complete nucleotide sequence of amyR2 and amyEn+ has been determined. Starting from an ATG initiator codon, there was an open reading frame comprising 477 amino acids (1,431 bp), giving a molecular weight of 52,678. The NH2-terminal portion of amyEn+ encoded a 41-amino acid-long signal sequence. The DNA nucleotide sequence was compared with the sequences of amyEm+ coding for M-type alpha-amylase from B. subtilis NA64 (Yamazaki et al. (1983) J. Bacteriol. 156, 327-337) and another B. subtilis alpha-amylase gene (Yang et al. (1983) Nucl. Acids Res. 11, 237-249). Almost all the sequences were identical in the three genes. However in the sequence of amyEn+ 32 bp of the other two alpha-amylase genes were deleted in the region from nucleotide 1,406 to 1,437. This deletion region was included in the direct repeat structure of the two genes. The reading frame downstream of the deletion region of amyEn+ shifted and a new termination codon (TGA) appeared at 26 bp downstream. Thus, the differences of the M-type and N-type alpha-amylases from amyEm+ and amyEn+ seemed to be caused by the occurrence of translation termination at different sites of the alpha-amylase gene.

Bacillus subtilis↗
PubMed1984

Amegakaryocytic thrombocytopenia associated with systemic lupus erythematosus successfully treated by a high-dose prednisolone therapy.

A patient with systemic lupus erythematosus (SLE) developed amegakaryocytic thrombocytopenic purpura. The absence of a drug relationship or other identifiable underlying cause made us consider this to be a rare manifestation of SLE. Her thrombocytopenia, which initially appeared to be steroid-resistant, ultimately improved following a prolonged high-dose prednisolone therapy. To our knowledge, this is the second case of amegakaryocytic thrombocytopenia associated with SLE in the literature. The possibility that amegakaryocytic thrombocytopenia in SLE can be caused by cellular rather than humoral immunologic mechanism was discussed.

Bone Marrow↗
PubMed1984

[Relationship between CT findings and consciousness in patients with hypertensive putamen hemorrhage].

The authors studied 66 patients with hypertensive putamen hemorrhage and noted a close relationship between the CT classification according to the shape of the suprasellar cistern (S) and the third ventricle (III), and the state of altered consciousness: when S is not deformed and III is identified in CT images, the patient may be in a clear or confusional state; if S is not deformed but III is not identified or is packed with hematoma, consciousness may be somnolent or stuporous; if S is deformed and III is not identified or is packed with hematoma, the patient may be in a semicomatose or comatose state. However, within several hours after onset there are exceptional cases in which the states of consciousness do not correlate with the CT findings. In these cases, however, with conservative therapy the state of consciousness corresponded well with the CT findings. From the neurosurgical points of view, immediate removal of the hematoma is required when the patient's consciousness is relatively good despite of large hematoma. If at the acute stage the state of consciousness, which corresponded well with the CT findings at initial examination, has deteriorated, rebleeding or enlargement of hematoma must be suspected.

Brain↗
PubMed1983

Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.

The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.

Amino Acid Sequence↗
PubMed1983

Enzymatic synthesis of flavin adenine dinucleotide.

A new and simple enzymatic method for the synthesis of flavin adenine dinucleotide (FAD) from flavin mononucleotide by the transadenylylation reaction using microbial cells is described. Among various microorganisms tested, Artherobacter globiformis IFO 12138 and two soil bacteria were selected as useful enzyme sources. Under suitable reaction conditions, the amount of FAD synthesized was 2.25 mumol/mL with cells of A. globiformis. The transadenylylation reaction could be coupled with the ATP supplying system through a glycolysis process with yeast.

Arthrobacter↗