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Biomedical subjects

K Wu

Publications and source records attributed to K Wu.

At least 271 records · Page 15Linked to original sources
PubMed1991

Gender recognition from speech. Part II: Fine analysis.

The purpose of this research was to investigate the potential effectiveness of digital speech processing and pattern recognition techniques for the automatic recognition of gender from speech. In part I Coarse Analysis [K. Wu and D. G. Childers, J. Acoust. Soc. Am. 90, 1828-1840 (1991)] various feature vectors and distance measures were examined to determine their appropriateness for recognizing a speaker's gender from vowels, unvoiced fricatives, and voiced fricatives. One recognition scheme based on feature vectors extracted from vowels achieved 100% correct recognition of the speaker's gender using a database of 52 speakers (27 male and 25 female). In this paper a detailed, fine analysis of the characteristics of vowels is performed, including formant frequencies, bandwidths, and amplitudes, as well as speaker fundamental frequency of voicing. The fine analysis used a pitch synchronous closed-phase analysis technique. Detailed formant features, including frequencies, bandwidths, and amplitudes, were extracted by a closed-phase weighted recursive least-squares method that employed a variable forgetting factor, i.e., WRLS-VFF. The electroglottograph signal was used to locate the closed-phase portion of the speech signal. A two-way statistical analysis of variance (ANOVA) was performed to test the differences between gender features. The relative importance of grouped vowel features was evaluated by a pattern recognition approach. Numerous interesting results were obtained, including the fact that the second formant frequency was a slightly better recognizer of gender than fundamental frequency, giving 98.1% versus 96.2% correct recognition, respectively. The statistical tests indicated that the spectra for female speakers had a steeper slope (or tilt) than that for males. The results suggest that redundant gender information was imbedded in the fundamental frequency and vocal tract resonance characteristics. The feature vectors for female voices were observed to have higher within-group variations than those for male voices. The data in this study were also used to replicate portions of the Peterson and Barney [J. Acoust. Soc. Am. 24, 175-184 (1952)] study of vowels for male and female speakers.

Computer Graphics↗
PubMed1991

Fraction of hepatic cytosolic acetyl-CoA derived from glucose in vivo: relation to PDH phosphorylation state.

We measured the contribution of glucose to hepatic cytosolic acetyl-CoA in vivo in rats and compared it with the phosphorylation state of a potentially regulatory enzyme complex [pyruvate dehydrogenase (PDH)]. Xenobiotic probes were used to sample hepatic cytosolic acetyl-CoA [acetylated sulfamethoxazole (SMX)] and UDP-glucose (glucuronidated acetaminophen) in vivo during [U-14C]glucose infusions. Percent active (dephosphorylated) form of PDH (PDHa) was determined on freeze-clamped liver. First, we confirmed using liver cell elutriation that acetylation of SMX occurs in parenchymal hepatocytes. Next, the fraction of cytosolic acetyl-CoA derived from [14C]glucose in vivo was shown to depend on dietary state. Specific activity of acetyl-CoA relative to plasma glucose or hepatic UDP-glucose was lower after 48 h fasting than after overnight fasting, and glucose refeeding (25 mg.kg-1.min-1 iv) maximally increased [14C]-glucose fractional contribution to acetyl-CoA within 2 h in the overnight-fasted but not in the prolonged fasted group. Hepatic PDHa demonstrated a similar but not identical pattern. The isotopic and enzymatic parameters showed significant correlations (r2 = 0.61 in 48-h fasted-refed group, r2 = 0.28 in overnight-fasted refed group), although [14C]glucose contribution to acetyl-CoA increased disproportionately compared with PDHa as refeeding progressed. The indirect pathway of UDP-glucose synthesis correlated inversely with the fractional contribution of glucose to acetyl-CoA. In summary, the fraction of hepatic acetyl-CoA derived from glucose in vivo is influenced by acute and chronic dietary factors and is only partially explained by PDHa. Regulation of the carbon source of hepatic acetyl-CoA in vivo and interactions suggested by these results (e.g., glucose-fatty acid cycle; branch-point regulation of glucose recycling) can be addressed in a quantitative fashion using this experimental framework.

Acetyl Coenzyme A↗
PubMed1991

Use of mass isotopomer distributions in secreted lipids to sample lipogenic acetyl-CoA pool in vivo in humans.

Measurement of hepatic fatty acid (FA) and cholesterol synthesis has been limited by lack of access to the precursor pool, cytosolic acetyl-CoA. We present a method for inferring the enrichment of the true hepatic lipogenic precursor pool in humans using the frequency distribution of mass isotopomers within enriched circulating polymers of acetyl-CoA [very low-density lipoprotein (VLDL)-palmitate, VLDL-stearate]. Human subjects were infused intravenously (n = 16) with [1-13C]- or [2-13C]acetate. Oral sulfamethoxazole (SMX) was administered concurrently, and the acetylated conjugate (SMX acetate) was used to estimate independently the hepatic cytosolic acetyl-CoA enrichment. Isotopomer frequencies in VLDL-FA were determined by gas chromatography-mass spectrometry, whereas high-performance liquid chromatography-mass spectrometry was used to measure enrichments in SMX acetate. Based on the excess M2/excess M1 ratio in VLDL-FA, calculated acetyl-CoA enrichments were 5.59 +/- 0.33 molar percent excess (MPE), whereas SMX acetate enrichments were 5.38 +/- 0.31 MPE (the 2 methods were not significantly different). Mass isotopomer-calculated and SMX acetate-measured estimates of acetyl-CoA enrichments correlated very closely in individual subjects (r2 = 0.93; P less than 0.0001). De novo hepatic lipogenesis can be measured using isotopomer-calculated precursor enrichments compared with measured incorporation in specific isotopomers of VLDL-FA. In summary, excess isotopomer frequencies in secreted lipids provide a non-invasive technique for estimating hepatic cytosolic acetyl-CoA enrichments in humans in vivo and correlate closely with enrichments observed using the xenobiotic probe technique. Isotopomeric distributions represent a new strategy for accurate measurement of macromolecule synthesis that may be applicable to other classes of molecules besides lipids.

Acetates↗
PubMed1991

Measurement of de novo hepatic lipogenesis in humans using stable isotopes.

Direct measurement of de novo lipogenesis has not previously been possible in humans. We measured de novo hepatic lipogenesis in normal men by means of stable isotopes and by combining the acetylated-xenobiotic probe technique with mass isotopomer analysis of secreted very low density lipoprotein-fatty acids (VLDL-FA). Sulfamethoxazole (SMX) was administered with [13C]acetate during an overnight fast followed by refeeding with intravenous glucose (7-10 mg/kg of weight per min), oral Ensure (7-10 mg of carbohydrate/kg of weight per min), or a high-carbohydrate mixed-meal breakfast (3.5 g of carbohydrate/kg of weight). Respiratory quotients remained less than 1.0. High-performance liquid chromatography/mass spectrometry-determined enrichments in SMX-acetate attained stable plateau values, and hepatic acetyl-coenzyme A (CoA) dilution rate did not increase with refeeding (approximately 0.024 mmol/kg per min). The fraction of VLDL-palmitate derived from de novo lipogenesis was only 0.91 +/- 0.27% (fasted) and 1.64-1.97% (fed). For stearate, this was 0.37 +/- 0.08% and 0.47-0.64%. Precursor enrichments predicted from isotopomer ratios were close to measured SMX-acetate enrichments, indicating that SMX-acetate samples the true lipogenic acetyl-CoA pool. Stearate synthesis was less than palmitate and the two did not move in parallel. Estimated total VLDL-FA synthesis is less than 500 mg/day. Thus, de novo hepatic lipogenesis is a quantitatively minor pathway, consistent with gas exchange estimates; fatty acid futile cycling (oxidation/resynthesis) is not thermogenically significant; and synthesis rates of different nonessential fatty acids by human liver are not identical in nonoverfed normal men. The contribution and regulation of de novo lipogenesis in other settings can be studied using this technique.

Acetyl Coenzyme A↗
PubMed1991

[Change of T lymphocyte subsets in peripherial blood of children with malnutrition and zinc deficiency].

Sixty children with malnutrition were investigated in this study. The results showed a reduction of serum zinc in moderate and severe cases compared with the mild and normal groups. OKT3+, OKT4+ and OKT8+ cells in peripheral blood of mild malnutrition were significantly decreased in contrast to normal control. The reductive degree of OKT8+ and OKT4+ cells correlated with the severity of malnutrition. OKT8+, OKT4+ and OKT4+/OKT8+ ratio of moderate and severe malnutrition with infection were much lower than those without infection. Our findings suggested that main effective and regulative cells of cell-mediated immunity were impaired. OKT3+ and OKT4+ cells might be used as a sensitive index for early assessment of the immune function in malnutrition children.

CD4-CD8 Ratio↗
PubMed1991

[Apolipoproteins AI, B100, CII, CIII and E in human cerebrospinal fluid].

Apolipoproteins AI, B100, CII, CIII and E contents were measured by radial immunodiffusion assay kits developed by our Research Unit. The apolipoprotein in 10 human cerebrospinal fluid samples was determined. Results show that there are no apo B100, CII or trace apo CIII in human cerebrospinal fluid, the levels of apo AI in cerebrospinal fluid are 1.00 +/- 0.54 mg/dl with a range of 0.35-2.00 mg/dl and the apo E content is 0.69 +/- 0.16 mg/dl with a range of 0.51-0.99 mg/dl. The results are discussed.

Apolipoprotein A-I↗
PubMed1991

[Effect of human fibroblast derived inhibitor on tumor and transformed cell proliferation and differentiation].

The effect of HFDI (human fibroblast derived inhibitor) on cell growth inhibition and differentiation of J6-1 (leukemia cell line). Namalva (Burkitt's lymphoma cell line), and fresh leukemic bone marrow cell cultures was studied. HFDI was found to inhibit tumor and leukemic cell growth as assayed by 3H-TdR incorporation, cell degeneration and necrosis, and to induce differentiation. We demonstrated that the characteristics of PHA-inhibited lymphocyte transformation were, in fact, identical to those of HFDI-inhibited degeneration and necrosis and HFDI-induced differentiation. It seems that necrosis was related to target cell sensitivity to HFDI.

Bone Marrow↗
PubMed1990

Liquid chromatography/mass spectrometry of plasma glucose and secreted glucuronate for metabolic studies in humans.

Negative ion thermospray liquid chromatographic/mass spectrometric methods have been developed for the determination of isotopic enrichments for glucose and acetaminophen-uridine diphosphate glucuronate from (1-2H1)glucose, (1-2H1)galactose and (2-13C)acetate in humans. The error of estimate ranged from below 1% to 5%. The advantages of the method include fast analysis (up to 35 per hour), eased sample preparation, good precision, sensitivity comparable with gas chromatography/mass spectrometry and better than with isotope ratio mass spectrometry.

Blood Glucose↗
PubMed1990

Effect of septal kindling on glutamate binding and calcium/calmodulin-dependent phosphorylation in a postsynaptic density fraction isolated from rat cerebral cortex.

Postsynaptic density (PSD) fractions were isolated from the cerebral cortices of control and kindled rats and assayed for glutamate and gamma-aminobutyric acid-binding capacities and for the Ca2+/calmodulin-dependent protein kinase. Glutamate binding was found to be increased by approximately 50% in the PSDs isolated from kindled rats as compared to controls; this increase was almost completely from an increase in Bmax; Kd decreased only slightly. Studies with inhibitors indicate that the receptors involved were of the N-methyl-D-aspartate and quisqualate types. PSDs isolated from control and kindled rats did not differ in gamma-aminobutyric acid or flunitrazepam binding. The in vitro autophosphorylation of the Ca2+/calmodulin-dependent protein kinase was depressed by 45-76% in PSDs isolated from kindled rats as compared to controls, with little change in amount of the kinase. Therefore, we infer that (i) the kindled state is associated with an increase in glutamate activation of postsynaptic sites, allowing Ca2+ to enter dendritic spines, (ii) a change has occurred in activity of the protein kinase, which is the major cerebral cortex PSD protein, and (iii) perhaps major alterations in the PSD are a concomitant to the long-lasting nature of the kindled state.

Animals↗
PubMed1990

[Comparative ability of three aniline derivatives to alter biotransformation enzymes in rat liver].

The effect of 4,4'-methylene bis (2-chloroaniline) (MOCA), 4,4'-methylene dianiline (MDA) and 4,4'-sulfonyl dianiline (Dapsone) on xenobiotic biotransformation in vivo was studied in male rat liver. Treatment with MOCA and MDA caused a dose dependent increase in ethoxyresorufin O-deethylase activity and concomitant decrease in aldrin epoxidase activity. Treatment with MOCA and MDA also resulted in increases in ethoxycoumarin O-deethylation and epoxide hydrolation while only MOCA induced cytosolic glutathione S-transferase activity. Treatment with Dapsone resulted in no changes in xenobiotic biotransformation except for the induction of aniline hydroxylation. The results are consistent with the contention that there is a relationship between carcinogenic chemicals and particular alteration in the activities of biotransformation enzymes.

Aniline Compounds↗
PubMed1990

[Role of "qi" in reaching affected area using acupuncture in "promoting blood circulation to remove blood stasis"].

In the present work, an experimental observation was carried out with the determination method of bioelectric impedance in 78 cases of chronic hepatitis, 58 cases of chronic obstructive pulmonary disease, 45 of obstructive thromboendarteritis and 65 of coronary heart disease, who were diagnosed definitely and possessed the symptoms and signs of blood stasis and produced change in hemodynamics. Yanglingquan (G. B. 34) and Zhangmen (Liv.13) acupoints on right side were needled and four indices of liver blood circulation were determined for chronic hepatitis. Bilateral Kongzui (Lu.6) acupoints were needled and seven indices of lung blood circulation were detected for chronic obstructive pulmonary disease. Acupoint along the pathway of channel were needled and six indices of blood circulation in the extremities were examined for obstructive thromboendarteritis. Bilateral Neiguan (P.6) acupoints were needled and four indices of the cardiovascular function were detected for coronary heart disease. Results showed 95 cases of the above four diseases having Qi reaching to affected area. 84 cases had propagated sensation along the channel. 67 cases had local sensation. Experimental results indicated that Qi reaching affected area produced obvious effects of dredging the meridian passage, and promoting blood circulation to remove blood stasis.

Acupuncture Therapy↗
PubMed1989

The Na+/Pi-cotransporter of OK cells: reaction and tentative identification with N-acetylimidazole.

Using an established renal epithelial cell line (OK cells) the effect of the amino-acid side-chain modifying reagent N-acetylimidazole (NAI) upon the sodium-dependent transport of phosphate (Pi) was investigated. After an incubation with 10 mM NAI for 20 min, cellular Na+/Pi uptake was inhibited by 70%. The presence of 5 mM Pi protected this transport function from being affected by NAI by 80 to 100%. Since the presence of sulfate was unable to protect the Na+/Pi transport inactivation by NAI and since the presence of Pi did not affect NAI inhibition of other transport systems, it is suggested that NAI interacts with the Pi transporter directly. The protective effect of Pi was used as a criterion to identify Pi-protectable [3H]NAI labelling of OK cell plasma membrane proteins. Pi protection was observed in four molecular mass regions: 31, 53, 104 and 176 kDa. Since the incorporation of [3H]NAI into these proteins was also affected by parathyroid hormone at 10(-10) M, it is concluded that the identified proteins represent possible candidates for the renal Na+/Pi cotransporter.

Animals↗
PubMed1989

Genotoxicity and effects on rat liver drug-metabolizing enzymes by possible substitutes for 4,4'-methylene bis(2-chloroaniline).

The mutagenic properties of Ethacure 300, Cyanacure and Polacure 740M, all possible substitutes for the industrial carcinogen, 4,4'-methylene bis(2-chloroaniline), have been determined in Salmonella typhimurium strains TA100, TA98, TA1535 and TA1537. These data have been compared with the effects of these chemicals on ethoxyresorufin O-deethylase (EROD) activity and aldrin epoxidase (AE) activity in rat liver. Ethacure 300 was clearly positive in both TA100 and TA98 bacterial strains, while Cyanacure was positive only in TA100. Polacure 740M was negative in all strains. Ethacure 300 caused a 28-fold induction of EROD while Cyanacure caused a doubling. Polacure 740M was without effect. Neither Ethacure 300 nor Cyanacure affected AE, while Polacure 740M caused an increase at only the lower dose tested. Thus there was excellent correlation between mutagenicity and EROD induction. A similar correlation was noted for six other structurally related compounds giving support to the contention that the ability of a chemical to induce EROD bears some relationship to its carcinogenic potential.

4-Aminobenzoic Acid↗
PubMed1989

Enhanced production of interleukin 1-beta by mononuclear cells isolated from mucosa with active ulcerative colitis of Crohn's disease.

IL1-beta production by mononuclear cells isolated from normal and active inflammatory bowel disease mucosa was studied. Significantly more IL1-beta was produced spontaneously by mononuclear cells from the inflamed mucosa compared with those from normal colonic mucosa (median 190 pg/ml (range 45-700) v 20 pg/ml (0-165)). Stimulation with lipopolysaccharide enhanced IL1-beta production by mononuclear cells from active inflammatory bowel disease mucosa but not those from normal mucosa. Depleting the mononuclear cells of macrophages, by panning with monoclonal antibody 3C10, reduced the amount of IL1-beta produced. Enhanced IL1-beta production from the inflamed mucosa may play an important role in the mediation of many inflammatory responses. The enhanced production appears to be the result of a recruited population of cells.

Cells, Cultured↗
PubMed1989

Interleukin-1-induced anorexia in the rat. Influence of prostaglandins.

The anorexia associated with acute and chronic inflammatory or infectious conditions is poorly understood. Our objectives were to explore the anorexigenic effects of interleukin-1 (IL-1) in the rat. Recombinant human (rh) IL-1 beta, murine (rm) IL-1 alpha and to a lesser extent rhIL-1 alpha significantly reduced food intake at greater than or equal to 4.0 micrograms/kg i.p. but not at lower doses, in young (200-250 g) meal-fed rats on chow diets. The anorexic effect appears to be mediated by prostaglandins since pretreatment with ibuprofen completely blocked it, and a fish oil based diet abolished it, in comparison to corn oil or chow diets. Fish oil feeding also decreased basal and IL-1 stimulated prostaglandin E2 production by tissues in vitro (liver, brain, peritoneal macrophages) and in the whole body. Constant intravenous infusions of lower doses of IL-1 also diminished food intake, though intravenous boluses did not (reflecting rapid renal clearance). Chronic daily administration of IL-1 caused persistent inhibition of food intake for 7-17 d in chow and corn oil fed rats, but had no effect in fish oil fed rats. There was an attenuation of the effect (tachyphylaxis) after 7 d in corn oil and chow fed rats, but slowed weight gain and lower final weights were observed after 17-32 d of daily IL-1. Old (18-20 mo Fisher 344) rats showed less sensitivity to IL-1 induced anorexia. In conclusion, IL-1 is anorexigenic in the rat, but this is influenced by the structural form of IL-1, the route and chronicity of administration, the source of dietary fat, and the age of the animal. The ability of prior fat intake to influence the anorexic response to IL-1 represents a novel nutrient-nutrient interaction with potential therapeutic implications.

Animals↗
PubMed1989

Effects of mutagenic and non-mutagenic aniline derivatives on rat liver drug-metabolizing enzymes.

1. The effect of 4,4'-methylene bis(2-chloroaniline) (MOCA), 4,4'-methylene dianiline (MDA) and 4,4'-sulphonyldianiline (Dapsone) in vivo on xenobiotic biotransformation in male rat liver was studied. 2. Treatment with MOCA or MDA but not Dapsone caused a dose-dependent increase in ethoxyresorufin O-deethylase activity and a concomitant decrease in aldrin epoxidase activity in male rats. 3. Treatment with MOCA or MDA resulted in dose-dependent increases in ethoxycoumarin O-deethylation and epoxide hydrolation, while only MOCA induced cytosolic glutathione S-transferase activity. 4. Treatment with Dapsone resulted in no changes in xenobiotic biotransformation except for the induction of aniline hydroxylation. 5. The results are consistent with the contention that there is a relationship between carcinogenic chemicals and particular alterations in the activities of biotransformation enzymes.

7-Alkoxycoumarin O-Dealkylase↗