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K Wu

Publications and source records attributed to K Wu.

At least 253 records · Page 14Linked to original sources
PubMed1992

Light and electron microscopic localization of alpha subunits of GTP-binding proteins, G(o) and Gi, in the cerebral cortex and hippocampus of rat brain.

Antibodies that recognize alpha subunits of G(o), Gi2 and Gi3 were used to evaluate their association with synaptic junctions. G(o), but not Gi, was concentrated within perikaryal and dendritic cytoplasm of a small population of bipolar neurons. All three G-proteins were associated with the intracellular surface of dendritic, axonal and astrocytic plasma membranes and postsynaptic densities (PSDs). However, association with PSDs was more prevalent for the two Gi's than for G(o) while the association with terminals forming putatively excitatory synapses was more prevalent for G(o) and Gi3 than for Gi2. Thus, neuromodulators may modulate the release of excitatory transmitters via activation of presynaptic Gi3 and G(o) and also regulate the opening of Ca2+ and/or K+ channels via activation of Gi's and G(o) at PSDs.

Animals↗
PubMed1992

Occurrence of the alpha subunits of G proteins in cerebral cortex synaptic membrane and postsynaptic density fractions: modulation of ADP-ribosylation by Ca2+/calmodulin.

We have examined the isolated postsynaptic density (PSD) fraction for the presence of a G protein. First, we found specific binding of guanosine 5'-[gamma-[35S]thio]triphosphate to the PSD. Second, pertussis toxin-activated ADP-ribosylation of the isolated PSD fraction resulted in the appearance of a G protein with an apparent molecular mass of 41 kDa, and two G proteins with apparent molecular masses of 41 kDa and 39 kDa in synaptic membrane (SM) fraction and total homogenate (H). The amount of the 41-kDa G protein per unit protein was in the order of SM greater than H greater than PSD. Anti-G(i0 antibodies recognized the 41-kDa G protein in both PSD and SM, whereas anti-G(o) antibodies reacted with the 39-kDa G protein in the SM. The absence of G(o) protein in the PSD suggested that there was no contamination with SM. Moreover, unlabeled PSD incubated with an extract of SM that contained the labeled G proteins resulted in no label in the subsequently reisolated PSD, suggesting that the G protein found in the PSD was not due to adsorption of the G protein onto the PSD during its isolation from the SM. PSD pretreated with EGTA gave an 11-fold increase in the ADP-ribosylation reaction of the G(i) protein; similar effects on the G(i) and G(o) proteins of SM were obtained. Restoration of Ca2+/calmodulin to the PSD, but not of either Ca2+ or calmodulin alone, removed the effect of EGTA, indicating a strong complex formation between G(i) and Ca2+/calmodulin that decreased the ADP-ribosylation reaction. Preincubation with the Ca(2+)-channel blocker nifedipine decreased the ADP-ribosylation reaction in the PSD. We conclude that G(i) is present in the PSD, that it may interact with calmodulin and that it is involved in the regulation of voltage-dependent Ca2+ channel. We present a theory of the involvement of the G protein and calmodulin in postsynaptic neurophysiological events.

Adenosine Diphosphate Ribose↗
PubMed1992

Molecular cloning of the transmembrane component of the 13762 mammary adenocarcinoma sialomucin complex. A new member of the epidermal growth factor superfamily.

Ascites sublines of the 13762 rat mammary adenocarcinoma have a cell surface sialomucin complex composed of the sialomucin ascites sialoglycoprotein-1 (ASGP-1) and the membrane-associated glycoprotein ASGP-2. The sialomucin complex is synthesized as a high M(r) precursor, pre-sialomucin complex (pSMC-1). To characterize the structure of the membrane-associated component of this complex, a lambda gt11 cDNA expression library was constructed using mRNA from 13762 rat mammary adenocarcinoma cells and screened with polyclonal antibody against ASGP-2. The strongest antibody-binding clone, designated lambda ASGP2.9-1, had a 1.3-kilobase (kb) insert, and hybridized to a 9-kb transcript in 13762 cell mRNA. The large size of this transcript was expected, since the estimated molecular mass of pSMC-1 is greater than 250 kDa. To obtain the full sequence of ASGP-2, a longer cDNA (5.4 kb), designated pASGP1/2.1, was subsequently cloned by screening a plasmid library with an oligonucleotide complementary to the 5' end of the phage insert. The amino acid sequence derived from nucleotide sequence of pASGP1/2.1 showed a 12-amino acid identity with amino acid sequence obtained from the NH2 terminus of ASGP-2, indicating the entire ASGP-2 coding region was included in the cDNA. Furthermore, an 18-amino acid identity with the NH2 terminus of a 6-kDa CNBr fragment of ASGP-2 was also observed in the cDNA sequence. The polypeptide contains several distinct domains, including a hydrophobic transmembrane domain, a short (20 residue) COOH-terminal cytoplasmic tail, and a large extracellular domain with 24 potential N-glycosylation sites. These properties correspond to features of ASGP-2 and pSMC-1 predicted by previous biochemical studies. Most interestingly, the extracellular domain contains two cysteine-rich sequences, each of which has a segment with strong similarities to proteins with epidermal growth factor activity. Since our recent studies show that ASGP-2 can modulate epidermal growth factor receptor phosphorylation activity, these results provide structural evidence to support the role of the heterodimeric sialomucin complex as a bifunctional modulator of cellular interactions and cell proliferation.

Adenocarcinoma↗
PubMed1992

Effects of recombinant monokines on hepatic pyruvate dehydrogenase, pyruvate dehydrogenase kinase, lipogenesis de novo and plasma triacylglycerols. Abolition by prior fasting.

1. The effects of recombinant human tumour necrosis factor alpha (TNF) and murine interleukin-1 alpha (IL-1) on the activation state of the hepatic pyruvate dehydrogenase complex (PDHa), the activity of mitochondrial PDH kinase, hepatic lipogenesis de novo and plasma triacylglycerol (TG) concentrations were studied. 2. Monokine effects depended upon prior nutritional state. In rats fasted for 20 h or 45 h before monokine administration and refeeding (orally or with intravenous glucose), PDHa, TG and hepatic lipogenesis were not increased. In rats fed ad libitum, treatment with TNF plus IL-1 increased the contribution of hepatic lipogenesis to circulating TG to 550% of control values (P = 0.03) and plasma TG concentrations to 159% (P = 0.02), whereas PDHa increased slightly to 120% (P = 0.02) and liver glycogen content fell to 45.8% (P = 0.05) of control values. 3. Intrinsic hepatic PDH kinase activity was not changed by monokine treatment in rats fed ad libitum. 4. The increased lipogenesis de novo showed no correlation (r2 = 0.05, not significant) with hepatic PDHa in individual animals fed ad libitum. 5. In conclusion, these results suggest that monokines increase pyruvate flux through hepatic PDH in vivo in rats fed ad libitum primarily by mechanisms other than covalent modification of PDH. Prior nutritional status exerts a permissive effect for monokine stimulation of PDHa and lipogenesis, consistent with a substrate-mediated action, but the mechanism of this permissive effect remains uncertain.

Animals↗
PubMed1992

On the identity of the major postsynaptic density protein.

Increasing evidence suggests that the postsynaptic density (PSD) plays a critical role in synaptic communication and plasticity. The major PSD protein (mPSDp), a calcium/calmodulin-dependent protein kinase, appears to be central to PSD function. The mPSDp has long been considered identical to the alpha subunit of the soluble calmodulin kinase II (alpha-CKII). However, mPSDp and alpha-CKII do differ in solubility and antigenicity, raising the possibility that the two proteins are distinct. To further define the relationship between the two proteins, we purified the mPSDp to homogeneity from adult rat cerebral cortex and compared the proteins. In contrast to alpha-CKII, the purified mPSDp was insoluble in high concentrations of salt, various detergents, chelators of divalent cations, and the strong denaturant guanidine hydrochloride. The pI value of the mPSDp was 6.2, whereas that of alpha-CKII was 6.7-7.2. The purified mPSDp bound calmodulin in the presence of Ca2+ and was autophosphorylated in a Ca2+/calmodulin-dependent manner. Polyclonal antiserum raised against mPSDp (anti-mPSDp) recognized purified mPSDp or mPSDp in synaptic membrane, indicating immunologic specificity among the synaptic proteins. Anti-mPSDp did not recognize alpha-CKII, whereas anti-alpha-CKII antibodies reacted only weakly with mPSDp, suggesting that the proteins are distinct but structurally similar. Moreover, sequence analysis of protease V8-digested polypeptides revealed that there was at least an 8-amino acid sequence, MLKVPNIS, that is not present in alpha-CKII. Finally, HPLC analysis of V8-digested fragments of mPSDp and alpha-CKII in parallel revealed dissimilar peptide patterns. Thus our observations suggest that mPSDp and alpha-CKII are similar but not identical. The unique physicochemical and structural properties of the mPSDp may provide insights into molecular mechanisms mediating synaptic plasticity.

Amino Acid Sequence↗
PubMed1992

Mechanisms of fructose-induced hypertriglyceridaemia in the rat. Activation of hepatic pyruvate dehydrogenase through inhibition of pyruvate dehydrogenase kinase.

1. The effects of purified diets containing 70% glucose or 70% fructose on the activation state of hepatic pyruvate dehydrogenase (PDHa), activity of mitochondrial PDH kinase, plasma triacylglycerols (TG) and hepatic lipogenesis de novo in rats were measured. 2. Plasma TG were significantly increased in the fructose-fed compared with the glucose-fed group (125 +/- 45 mg/dl versus 57 +/- 19 mg/dl; P less than 0.002) after 3-5 weeks on the diet despite less daily food intake. 3. Hepatic PDHa in fructose-fed rats was 144% of the value in glucose-fed rats (15.4 +/- 1.2% versus 10.7 +/- 0.5%; P less than 0.002), whereas cardiac muscle PDHa was not different (45.5 +/- 6.6% versus 41.0 +/- 7.8%). 4. Intrinsic hepatic PDH kinase activity was decreased to 34% of glucose-fed values by fructose feeding (-k = 3.56 +/- 0.39 versus 10.41 +/- 1.85 min-1; P less than 0.005). 5. The fractional contribution to very-low-density-lipoprotein palmitate from hepatic lipogenesis de novo, measured by a stable-isotope mass-spectrometric method, was 10.49 +/- 2.42% (n = 8) in fructose-fed rats versus 5.55 +/- 1.38% (n = 9) in glucose-fed rats (P less than 0.05), and 2.66 +/- 2.39% (n = 3) in chow-fed rats (P less than 0.05 versus fructose-fed group). The absolute contribution to circulating TG from lipogenesis de novo was also significantly higher in the fructose-fed than in the glucose-fed group (14.9 +/- 5.1 mg/dl versus 2.9 +/- 0.6 mg/dl; P less than 0.05) 6. Portal insulin concentrations were significantly higher in the fructose-fed rats (206 +/- 49 mu-units/ml versus 81 +/- 15 mu-units/ml; P less than 0.05). 7. In conclusion, dietary fructose appears to have a specific activating effect on hepatic PDH, mediated at least in part by inhibition of PDH kinase. These results are consistent with increased flux through hepatic PDH and synthesis of new fat, not just increased re-esterification of non-esterified fatty acids.

Animals↗
PubMed1992

Short-term intraindividual variability in hemostasis factors. The ARIC Study. Atherosclerosis Risk in Communities Intraindividual Variability Study.

Recent epidemiologic studies found that there is a strong association of hemostatic factors with ischemic heart disease. The Atherosclerosis Risk in Communities (ARIC) Intraindividual Variability (IIV) Study was conducted to estimate the various components of variation in hemostasis factors measured in the ARIC Study and to estimate the measures of repeatability of these factors. A total of 39 subjects (16 men, 23 women) were studied. Each had blood collected three times, with a 1- to 2-week interval between each visit. The contributions of between-person variability, within-person (biologic) variability, and processing and assay variability were estimated. Then the reliability coefficient R was estimated as the proportion of total variance accounted for by between-person variance. The reliability coefficient can be interpreted as the correlation between measures made at repeat visits. Among the various analytes, the reliability coefficients were quite high for activated partial thromboplastin time and plasma factor VIII (R = 0.92, 0.86, respectively). Low repeatability was obtained for antithrombin III activity and protein C (R = 0.42, 0.56, respectively). The lack of repeatability for these variables derives mostly from the processing (field center and laboratory) variation. Other analytes--fibrinogen, plasma factor VII, and von Willebrand factor--were intermediate in repeatability. In comparing the analyte-specific high-level to low-level groups, no substantial difference of within-person plus method coefficient of variation between the two groups was found for any analyte except for factor VIII, whereas the corresponding variance components for most analytes were higher for the higher analyte level. Reliability coefficients from this ARIC IIV study are generally higher than those found in other studies, and this is related to the relative variations in populations studied and to the time between measurements.

Antithrombin III↗
PubMed1992

Nuclear localization signals, but not putative leucine zipper motifs, are essential for nuclear transport of hepatitis delta antigen.

Hepatitis delta antigen (HDAg) is the only known protein of hepatitis delta virus and was previously shown to localize in the nucleoplasm of infected liver cells. In this study, nuclear localization signals of HDAg were defined by expressing various domains of the antigen in both hepatic and nonhepatic cells as beta-galactosidase fusion proteins. A cytochemical staining assay demonstrated that a domain from amino acid residues 35 to 88 of HDAg was able to facilitate transport to the nucleus of the originally cytoplasm-localized protein beta-galactosidase. Two nuclear localization signals, NLS1 and NLS2, which are similar to those of simian virus 40 T antigen and polyomavirus T antigen, respectively, were identified. Either NLS1 or NLS2 alone was sufficient for the nuclear transport of HDAg. However, a fusion protein (N65Z) containing beta-galactosidase and the N-terminal 65 amino acids of HDAg, containing NLS1, was localized exclusively in the cytoplasm and perinuclear region. A possible hydrophobic subdomain between amino acid residues 50 and 65 may block the function of NLS1. Nevertheless, N65Z could enter the nuclei of transfected cells when it was coexpressed with full-length HDAg. Entry into the nucleus may be mediated by the coiled-coil structure rather than the putative leucine zipper motif located between amino acid residues 35 and 65. The existence of two independent nuclear localization signals may ensure the proper functioning of HDAg in the multiplication of delta virus in the nucleus. In addition, two putative casein kinase II sites (SRSE-5 and SREE-126) that may be important in controlling the rate of nuclear transport were found in HDAg.

Amino Acid Sequence↗
PubMed1992

[Effects of zinc deficiency and vitamin D deficiency on bone calcification and development of rats].

Zinc deficiency rat model was made by feeding zinc deficiency diet. The level of bone calcium of the zinc deficiency rats was significantly lower than that of the control rats. Their bone cortex was thinner and bone density decreased. The counts of their cartilage cells and hypertrophic cells of epiphyseal plate were less frequent, and the diameter of their hypertrophic cells was smaller than that of the controls. It suggested that zinc deficiency caused defective bone calcification which was similar to that in vitamin D deficiency. Zinc deficiency seemed to hinder the linear growth of long bone and might be the cause of dwarf.

Animals↗
PubMed1992

[Studies on human gamma-crystallins. I. Quantitative changes with age and cataract formation].

Water soluble protein from human lenses at various ages are separated by Sephadex gel chromatography. The results show that there are major three gamma-crystallins (gamma 1, gamma 2, gamma 3) in human lenses. In normal lenses the total contents of the three gamma-crystallins in water soluble fraction of lens proteins keep stable as age increases. However, among the three gamma-crystallins, gamma 1 increase and gamma 2 decrease markedly, and very little change with gamma 3 are found as age increases. In nuclear cataractous lenses, all three gamma-crystallins decrease as compared with the normal lenses of the same age. The variety of synthesis of each gamma-crystallin is mainly responsible for the changes of their quantities. It is postulated that the total low molecular weight crystallin contents in water soluble proteins of the human lens is related to the transparency of lens. The structural characterization of gamma 3-crystallin is responsible for the relative constant as age increases.

Adolescent↗
PubMed1992

Potassium iodide and acrylamide fluorescence quenching studies on gamma-crystallins of human lenses in development and aging.

gamma 1-, gamma 2- and gamma 3-crystallin (corresponding to gamma s-, gamma C- and gamma D-crystallin respectively) of human fetal, 2 year and 20+ year old lenses are separated by Sephadex gel chromatography. Iodide and acrylamide are used to quench the tryptophane fluorescence of sub-gamma-crystalline fractions and Ksv and fa values are calculated. The results show that iodide has no clear quenching effects on all gamma-crystallins, the quenching effects of acrylamide on the tryptophan fluorescences of gamma 1-, gamma 2- and gamma 3-crystallin from lenses of the same age have little differences, and the quenching rates of tryptophane fluorescences of gamma 1-crystallin decreases and that of gamma 2- and gamma 3-crystallin increase with age. These results indicate that all tryptophan residues in these three gamma-crystallins lie in hydrophobic environments of molecule and their exposure degree is in the order gamma 2 < gamma 3 < gamma 1. The tryptophan exposure of gamma 2 and gamma 3 increase (gamma 2 > gamma 3) and of gamma 1 decrease with age. The age related change of gamma 1 may be related to the changing of structure or containing of new constituents. Although the tryptophan exposure in gamma 3 is higher than that in gamma 2, the change of structure with age in gamma 2 is more prominent than in gamma 3.

Acrylamides↗
PubMed1992

Comparative studies of the carbohydrate of human gamma-crystallins from fetal and adult lenses with agglutinins.

Using gel chromatography of Sephadex G-75 superfine connected with Sephadex G-50 fine column, three human gamma-crystallins (gamma 1, gamma 2, gamma 3) could be obtained. Seven agglutinins (LCA, SBA, DBA, PNA, BSL, RCA and UEA) were used to detect the sugar of sub-gamma-crystallins, which had been transferred to nitrocellulose membrane and finally stained with ABC reagents and the substrate of HPR. These results suggested that gamma 2- and gamma 3-crystallins contain sugar, but gamma 1-crystallin has no sugar. There is a decrease of carbohydrate of gamma 2 and gamma 3 as the development of the lens (esp. the former). It was postulated that in gamma-crystallins (gamma 2, gamma 3) the sugar components helped stabilizing the molecular structure (folding polypeptide, prevent protein breaking). The sugar components decreased with age by the post-translation modification.

Adult↗
PubMed1992

Quantitative risk assessment for lung cancer from exposure to metal ore dust.

To quantitatively assess risk for lung cancer of metal miners, a historical cohort study was conducted. The cohort consisted of 1113 miners who were employed to underground work for at least 12 months between January 1, 1960 and December 12, 1974. According to the records of dust concentration, a cumulative dust dose of each miner in the cohort was estimated. There were 162 deaths in total and 45 deaths from lung cancer with a SMR of 2184. The SMR for lung cancer increased from 1019 for those with cumulative dust dose of less than 500 mg-year to 2469 for those with the dose of greater than 4500 mg-year. Furthermore, the risk in the highest category of combined cumulative dust dose and cigarette smoking was 46-fold greater than the lowest category of dust dose and smoking. This study showed that there was an exposure-response relationship between metal ore dust and lung cancer, and an interaction of lung cancer between smoking and metal ore dust exposure.

Adult↗
PubMed1991

Sampling the lipogenic hepatic acetyl-CoA pool in vivo in the rat. Comparison of xenobiotic probe to values predicted from isotopomeric distribution in circulating lipids and measurement of lipogenesis and acetyl-CoA dilution.

The acetylation of xenobiotics may provide a means for sampling hepatic cytosolic acetyl-CoA in vivo for isotopic studies of lipogenesis. Here, we test the accuracy of acetylated-sulfamethoxazole (SMX) in representing the true precursor pool for hepatic lipogenesis by comparison to a mathematical technique for estimating acetyl-CoA enrichment using the mass isotopomer distribution in circulating lipids. We then go on to measure hepatic fatty acid synthesis in intact rats using stable and radioisotopes. Specific activities and enrichments of SMX-acetate (the latter determined by high performance liquid chromatography-mass spectrometry) were monitored during fasting and refeeding. The dilution rate of hepatic acetyl-CoA relative to infused 13C- or 14C-acetates was 0.158-0.200 mmol/kg body weight/min during fasting, and did not increase significantly in rats refed with intravenous glucose at 25-30 mg/kg/min or refed ad libitum with chow, suggesting little additional input of acetate units. Plasma beta-hydroxybutyrate specific activity was much lower than SMX-acetate. The isotopomeric frequency distributions in circulating very low density lipoprotein (VLDL)-palmitate and VLDL-stearate were used to estimate the enrichment of the true precursor, hepatic acetyl-CoA, from a model based on the binomial distribution. The calculated acetyl-CoA values (7.28 +/- 0.49 molar percent excess (n = 16] based on isotopomeric frequencies were very close to measured SMX-acetate enrichments (7.44 +/- 0.41 molar percent excess (n = 21] and values within individual animals (n = 14) correlated very well (r2 = 0.90, p less than 0.0001). The contribution of VLDL-fatty acid by the de novo lipogenic pathway was similar using the stable isotope approach or radioisotopes (only 1-2% in fasted or intravenous glucose refed rats, 5% in chow refed). Combining fractional de novo lipogenesis values with absolute de novo lipogenesis rates allows estimation of total VLDL-triglyceride synthesis. In conclusion, the xenobiotic acetylation technique provides continuous access to the lipogenic hepatic acetyl-CoA pool in vivo and permits measurement of fatty acid synthesis. Isotopomer ratios in secreted lipids provide another method for estimating true precursor acetyl-CoA enrichments.

Acetates↗
PubMed1991

Three MDBP sites in the immediate-early enhancer-promoter region of human cytomegalovirus.

MDBP, a mammalian sequence-specific DNA-binding protein, was found to recognize two sites in the major immediate-early (IE) enhancer of human cytomegalovirus. The recognition sequence for MDBP at each of these sites was localized to 14 bp by studying the effects of limited G methylation, depurination, depyrimidination, or deoxyribose modification on the ability of these sites to bind to MDBP. In addition to the two high-affinity MDBP sites in the enhancer, one low-affinity MDBP site was detected 5 bp after the transcription initiating residue of this IE transcription unit. The possible biological significance of the two enhancer MDBP sites and the downstream MDBP site is discussed.

Base Sequence↗
PubMed1991

Beneficial effects of N-acetylcysteine and cysteine in stunned myocardium in perfused rat heart.

1. The objective of this study was to evaluate the effects of three sulphydryl (SH) compounds, N-acetylcysteine (NAC), cysteine (Cys) and cystamine, on functional recovery and ventricular arrhythmias (VF) in stunned myocardium in the isolated perfused heart of the rat. 2. Hearts (n = 7-8 per group) were perfused by the Langendorff procedure for 20 min to stabilize and then assigned to one of five groups: saline, sham, NAC, Cys and cystamine. After the stabilizing period, the drugs (at 3.6 microM min-1) or their vehicle (saline) were infused into coronary vessels throughout the experimental period. Ten min after administration of drugs, the left anterior descending coronary artery (LAD) was ligatured for 20 min and then untied to reperfuse for 30 min. In the sham group, a ligature was placed around the LAD but not tied. 3. NAC and Cys had a significant effect in attenuating myocardial stunning: the percentage recovery of rate-pressure product measured 30 min after reperfusion as an index of heart function, was improved with the NAC (98.3 +/- 4.5) and Cys groups (104.0 +/- 6.5) compared with the saline (only 73.6 +/- 3.8, P < 0.01) group. Cystamine did not show these beneficial effects. This may be due to the difference in chemical structure between NAC, Cys and cystamine since the latter does not have a free SH group with a disulphide bond formed. This phenomenon suggests that a free SH group is essential for the protective effects of compounds like NAC and Cys in myocardial injury. 4. NAC and Cys prevented the fall in coronary flow during the LAD occlusion and enhanced coronary flow during reperfusion but cystamine did not have such a beneficial effect. 5. The incidence of VF in the saline, cystamine, Cys and NAC groups was 6/8 (75.0%), 4/7 (57.1%), 3/8 (37.5%) and 2/7 (28.6%), respectively, and no significant differences (P > 0.05) were noted between the saline- and drug-treated groups. 6. An in vitro study with electron spin resonance indicated that Cys effectively scavenged the hydroxyl radical (-OH) generated by Fenton's reaction but did not scavenge superoxide generated in an irradiated riboflavin system. NAC and cystamine showed a scavenging effect on -OH to a certain extent but this effect did not reach statistical significance (P > 0.05 vs saline). 7. Our results demonstrate that NAC and Cys treatment before ischaemia and reperfusion can reduce myocardial stunning. This beneficial effect may be mainly due to their ability to preserve and enhance coronary flow during coronary occlusion and reperfusion and in part due to scavenging -OH and/or replenishing intracellular glutathione. The results also indicate that the condition of coronary perfusion can produce a great impact on postischaemic ventricular performance.

Acetylcysteine↗
PubMed1991

Gender recognition from speech. Part I: Coarse analysis.

The purpose of this research was to investigate the potential effectiveness of digital speech processing and pattern recognition techniques for the automatic recognition of gender from speech segments. In this paper "coarse" acoustic coefficients (autocorrelation, linear prediction, cepstrum, and reflection) were used to form test and reference templates for vowels, voiced fricatives, and unvoiced fricatives. The effects of different distance measures, filter orders, recognition schemes, and vowels and fricatives were comparatively assessed to determine their effectiveness for the task of gender recognition from speech segments. The results showed that most of the acoustic parameters worked well for gender recognition. A within-gender and within-subject averaging technique was important for generating appropriate test and reference templates. The Euclidean distance measure appeared to be the most robust as well as the simplest of the distance measures. The results from this study implied that the gender information is time invariant, phoneme independent, and speaker independent for a given gender. One recognition scheme achieved 100% correct speaker gender classification for a database of 52 talkers (27 male and 25 female). In part II of this paper [D.G. Childers and K. Wu, J. Acoust. Soc. Am. 90, 1841-1856 (1991); hereafter referred to as paper II] the detailed features of ten vowels that appeared responsible for distinguishing a speaker's gender were examined statistically. Included in paper II is a replication of part of the classical study of Peterson and Barney [J. Acoust. Soc. Am. 24, 175-184 (1952)] of vowel characteristics.

Adult↗