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Biomedical subjects

K Worowski

Publications and source records attributed to K Worowski.

At least 37 records · Page 2Linked to original sources
PubMed1995

The effect of activated alveolar macrophages on experimental lung emphysema development. I. Protease and antiprotease activities in the culture medium of alveolar macrophages.

Aim of the present study was to evaluate cathepsin D, base protease, antiplasmin, antitrypsin and antichymotrypsin activities and protein content in the 24h culture medium of the alveolar macrophages (AM) deriving from the rats treated BCG-vaccine and from rats with papain-induced emphysema. In the culture medium of cells isolated from the rats which were given BCG or papain and BCG+papain we observed an increase of base protease activity and a decrease of cathepsin D activity comparing with control group. Increased antitrypsin activity in BCG and BCG+papain-treated rats and decreased antitrypsin activity in papain-treated rats were observed. There were not significant differences in antiplasmin and antichymotrypsin activities between examined groups. The obtained results indicate that activated pulmonary macrophages are one of the sources of the protease-antiprotease intraalveolar imbalance. However, increased production of proteolytic enzymes may not be the only factor responsible for the progression of lung emphysema in BCG-treated rats.

Animals↗
PubMed1994

Synthesis and activity of dipeptides containing epsilon-aminocaproic acid.

Five new dipeptides containing epsilon-aminocaproic acid were synthesized. All dipeptides markedly inhibit fibrinolytic activity of plasmin but only in low concentration (0.0002M). In higher concentration fibrinolytic activity was observed. Activity of dipeptides containing epsilon-aminocaproic acid, which antifibrinolytic activity is known, was tested on plasmin, thrombin, urokinase and euglobulin fraction using synthetic substrates.

Amides↗
PubMed1993

Complexoproductive and antiheparin properties of low density lipoproteins (LDL). III. Interaction of low density lipoproteins (LDL) with synthetic polyanionic compounds.

Low density lipoprotein (LDL) form insoluble complex bonds with polymetacrylic and polyvinylsulphonic acids in the presence of calcium chloride. Quantity of arising complexes depends on the concentrations of LDL, acids and calcium chloride and on pH. These complexes dissociate solutions of sodium chloride, urea and guanidine hydrochloride.

Calcium Chloride↗
PubMed1993

Complexoproductive and antiheparin properties of low density lipoproteins (LDL). V. Optimum conditions of the coagulation method to determine antiheparin activity in the blood plasma.

It was found that storing the plasma in the temperature 0 degree C, 4 degrees C and 20 degrees C and especially in 37 degrees C increases pH and decreases antiheparin activity estimated by the heparin-thrombin test. Measurements of the heparin-thrombin time should be carried out directly after obtaining the plasma or after reducing the plasma to pH 7.40.

Adult↗
PubMed1993

Complexoproductive and antiheparin properties of low density lipoproteins (LDL). VI. Antiheparin activity in blood plasma of different species of vertebrates.

Antiheparin activity of plasma of different species of vertebrates depends to a large extent on contents of low density lipoproteins (LDL). High antiheparin activity of the blood plasma of chicken and human corresponds to high contents of LDL and low antiheparin activity of the blood plasma of horse, cow, sheep, dog and pig corresponds to decreased contents of these proteins. Differences in the contents of fibrinogen, acid alfa1-glycoproteins, globulins, alkaline proteins and antithrombin III activity have smaller influence on antiheparin activity in the blood plasma of the examined animals.

Animals↗
PubMed1993

[Proteolytic and antiproteolytic activity of cholesteatoma and granulomatosis tissue of the middle ear].

Cholesteatoma and granulomatosis tissue show proteolytic activity measured in pH 7.6. Cholesteatoma activity was found to be higher than this of granulomatosis tissue. Cholesteatoma does not appear to poses antiplasmin activity. Antiplasmin granulomatosis tissue activity is slight. Both cholesteatoma and granulomatosis tissue show antitrypsin activity. Antitrypsin cholesteatoma activity is lower than that of granulomatosis tissue.

Cholesteatoma↗
PubMed1992

[Effect of different vascular prosthesis materials on blood platelets, blood clotting and fibrinolysis].

Studies were performed on Dacron vascular prostheses (USA), Gore-Tex (Germany), polyester prostheses (Czechoslovakia), Lawsan (Russia), as well as on DALLON-standard and DALLON-double velour prostheses (Poland), non-impregnated or impregnated with collagen, with heparin and albumin, with antithrombotic and antibacterial preparation, and with antibacterial preparation. In vitro, all the materials of vascular prostheses cause an adhesion of platelets in different degree, increase the availability of platelet factor 3, release platelet factor 4, reduce the blood clot retraction, shorten clotting time of the whole blood as well as recalcination time of intact plasma and increase the prothrombin consumption. None of the materials induced platelet aggregation nor influenced the activity of fibrinolytic system. Prostheses impregnated with collagen or with heparin and albumin were the most thrombogenic. Those impregnated with antibacterial preparation or with antibacterial and antithrombotic preparations, and Gore-Tex prostheses showed the lowest thrombogenicity. Thrombogenicity of vascular prostheses not only depends on chemical structure of the material, but on the method of fibre manufacturing by individual producers as well.

Animals↗
PubMed1992

[Changes in the activity of the clotting system and fibrinolysis components in the layers of double velour DALLON grafts at different periods after implantation into the aorta].

During one year experiment, the thromboplastic, antiheparin, plasminogen activator, antithrombin and antiplasmin activities were evaluated in the homogenates of the intimal, medial and adventitial layers of polyester double velour DALLON prostheses implanted into the dog abdominal aorta. It was found, that 7 days after implantation the thromboplastic, antiheparin and plasminogen activator activities in DALLON graft neointima were high, whereas those of the antiplasmin and antithrombin were low. These changes facilitate the sealing of the prosthesis pores and at the same time prevents thrombosis. Four months after implanting, the activity of hemostatically active components in the various graft layers became similar to the activity of aorta components. Rapid decrease of the thrombogenic potential and increase of the fibrinolytic activity in layers of double velour DALLON grafts facilitate the maintenance of graft patency.

Animals↗
PubMed1992

[Comparison of cathepsin D activity of DALLON-standard and DALLON-double velour grafts implanted into the abdominal aorta].

Cathepsin D activity was compared during the healing period of polyester DALLON-standard and DALLON-double velour prostheses implanted into the abdominal aorta of dogs. During first months following the implantation cathepsin D activity is considerably higher in all the layers of both types of prostheses than in the aorta. The normalization of this enzyme activity occurs significantly faster during the healing of DALLON-double velour prostheses than in that of DALLON-standard. This fact shows greater progress in the protein metabolism normalization during the process of healing and tissue reconstruction in DALLON-double velour prostheses.

Animals↗
PubMed1992

Complexoproductive and antiheparin properties of low density lipoproteins (LDL). I. Interaction of isolated low density lipoproteins with heparin of different molecular weight.

Low density lipoproteins/LDL/ form unsoluble complexes with heparin of different molecular weight in the presence of CaCl2. Quantity of the complexes depends on molecular weight of heparin, concentration of reagents and pH of the reacting medium. The biggest quantity of complexes result from LDL-heparin of the molecular weight 16000, the quantity is smaller with heparin of the molecular weight 5100 and the quantity is smallest with heparin of the molecular weight 3700. Components of LDL--heparin complexes are bound by means of ion and hydrogen bonds.

Calcium Chloride↗
PubMed1992

Complexoproductive and antiheparin properties of low density lipoproteins (LDL). II. Interaction of serum low density lipoproteins (LDL) with heparin of different molecular weight.

Quantity of unsoluble LDL complexes with heparin of different molecular weight in hypo-, normo-, and hyperlipemic blood serum in the presence of CaCl2 was estimated. At the same weight concentration the biggest quantity of LDL complexes are formed with heparin with heparin of the molecular weight 16000, the quantity is smaller with heparin of the molecular weight 5100, and it is the smallest with heparin of molecular weight 3700.

Calcium Chloride↗
PubMed1992

[Proteolytic enzymes of the digestive tract in ethanol poisoning].

The present paper reviews the literature on changes of proteolytic enzymes activity, disorders of protein digestion and absorption of protein degradation products from digestive tract in ethanol intoxication. Magnitude of the change depends on concentration, dose and time of ethanol consumption. Acute ethanol intoxication causes increase in gastric and pancreatic proteolytic enzymes secretion and reduces amino acids and peptides absorption. Chronic ethanol consumption results in reduced synthesis and secretion of gastric and pancreatic proteinases.

Alcohol Dehydrogenase↗
PubMed1992

[Metabolism of liver proteins in ethanol poisoning].

The present paper reviews the literature on influence of ethanol and acetaldehyde on synthesis, export and degradation of liver proteins. Direction and intensification changes caused by ethanol and acetaldehyde depend on concentration, time of activity and the way of administration of these compounds, and the way of feeding.

Acetaldehyde↗
PubMed1991

Cathepsin D activity of an aortic polyester double velour DALLON graft.

Cathepsin D activity, protein and acid-soluble tyrosine contents in a polyester double velour DALLON graft were evaluated in an experiment lasting one year, performed in 36 male mongrel dogs. The cathepsin D activity in the intimal, medial and adventitial layers of the graft on the 7th day of the experiment was much higher than in a normal artery and vein. In the intimal and medial layers of the graft, this activity increased in the 1st and 4th months of the experiment. In all the layers, cathepsin D activity fell to the level of the activity in normal vessels after one year of the experiment. The protein and acid-soluble tyrosine contents in all the graft layers were highest on the 7th day after which they decreased gradually.

Animals↗
PubMed1990

Peptide inhibitors of plasmin.

Seven peptides of the general formula A-Phe-Lys-X or A-Phe-NH-(CH2)5-NH2 where A = Glp or Ala, X = -OH, -NHC7H15 or -OC7H15 were obtained. The peptide H-Ala-Phe-Lys-NHC7H15 markedly inhibits the fibrinolytic activity of plasmin. The antifibrinolytic activity of the peptide Glp-Phe-Lys-OC7H15 is similar to EACA. The both peptides only slightly activate the caseinolytic activity of plasmin.

Caseins↗