Search PubMed⌕ Search

Biomedical subjects

K Wonigeit

Publications and source records attributed to K Wonigeit.

At least 199 records · Page 11Linked to original sources
PubMed1984

Phenotype and histological distribution of interstitial dendritic cells in the rat pancreas, liver, heart, and kidney.

The phenotype and the histological distribution of interstitial dendritic cells was investigated in the rat pancreas, liver, heart, and kidney by immunoperoxidase techniques. Monoclonal antibodies, directed against lymphocyte differentiation antigens (W3/25, W3/13, and Ox8) or against Ia antigens, revealed distinct phenotypes of these cells in all organs investigated--namely W3/25+Ia- dendritic cells, W3/25+Ia+ cells, and a small W3/25-Ia+ population. In the kidney cortex a W3/25+W3/13+ population was additionally shown. Interestingly, the phenotypically distinct cell populations also differed in their topographical distribution: W3/25+Ia+ cells were evenly scattered in the interstitium of the endocrine and exocrine pancreas, heart, and kidney. In contrast, W3/25+Ia- cells showed an additional predilection for connective tissue septa in the exocrine pancreas and for the kidney medulla. In the liver, phagocytic Kupffer cells were W3/25+Ia-, whereas the W3/25+Ia+ nonphagocytic dendritic cell population resided periportally and around central veins. These results show a marked heterogeneity of interstitial dendritic cells in the rat, which is discussed in terms of different cell lines or different activation or maturation stages of one single cell type.

Animals↗
PubMed1984

Membrane alterations in magnesium-deficiency-induced malignant T cell lymphoma.

Malignant T cell lymphoma cells induced in the thymus by chronic Mg deficiency showed a less ruffled surface in the scanning electron microscope, particularly after intraperitoneal transplantation. Their content of phosphatidylserine and phosphatidylinositol was increased. The content of phosphatidylcholine, cholesterol and the cholesterol/phospholipid ratio, and thus membrane microviscosity, were decreased. The activity of phospholipid methyltransferase I and II was reduced. In parallel to these alterations, 45Ca2+ influx and efflux as well as 45Ca2+ content in mitochondria, microsomes and cytosol of lymphoma cells were enhanced.

Animals↗
PubMed1984

Dosage of Cyclosporin A in children with renal transplants.

Results of Cyclosporin A (CyA) treatment following kidney transplantation in 16 children are reported. CyA was used in combination with low-dose prednisolone. The dosage of CyA was related to body surface area, starting with 500 mg/m2 daily and was reduced weekly by 50 mg/m2 until the maintenance dose of 300 mg/m2 was reached at the end of the fifth week. The dosage was controlled and adjusted by monitoring the CyA blood concentrations. In comparison with adults, children required higher CyA doses related to body weight to maintain the desired trough blood level range (200-750 ng/ml). In 16 children treated with CyA the graft function rate at three months was 100% and at six months 90%, because one patient died of septicemia. 10 patients experienced 20 reversible rejection episodes. Infectious complications and side-effects were similar to those observed in adults. Almost half of the patients exhibited transient nephrotoxicity which reversed after dose reduction. It is concluded that CyA treatment in a body surface area related dosage and in combination with blood level monitoring offers a successful way for kidney transplantation in childhood.

Adolescent↗
PubMed1984

Diagnostic concepts in clinical organ transplantation: histocompatibility testing and T cell monitoring.

Immunological rejection is still the most important problem in clinical organ transplantation requiring the lifelong immuno-suppressive treatment of the allograft recipient. The clinical success rate however can be improved by a variety of diagnostic procedures: The reduction of the histocompatibility barrier between donor and recipient by HLA-typing and organ exchange in order to obtain the best possible match; posttransplant monitoring of donor specific immune responsiveness towards donor antigens; monitoring of T-cell subsets by monoclonal antibodies and cytofluometry. T-cell subset monitoring is a rapidly developing new technique with great potential. With the presently available first generation reagents already a detailed analysis of the major T-cell population in circulation is possible, which can markedly facilitate the diagnosis of rejection and viral infection. New monoclonal antibodies, defining certain activation stages of T-cells or permitting a more refined differentiation of functionally distinct subsets will lead to increased relevance of this diagnostic approach. A particularly interesting aspect of monoclonal antibodies against T-cell differentiation antigens is their potential therapeutic use as immunosuppressive drugs with a highly selective action on defined T-cell subsets.

Antibodies, Monoclonal↗
PubMed1983

The subcapsular hydrostatic pressure of allogeneic kidney grafts: a new clinical method for postoperative transplant monitoring.

A method for the measurement of the subcapsular hydrostatic pressure in human kidney grafts is described. This method permits follow-up studies over periods up to 4 weeks. First results indicate that the method is a useful tool to monitor the condition of renal allografts in the early postoperative period. It allows the early recognition of acute rejection episodes and predicts their course and reversibility. The subcapsular hydrostatic pressure is a particularly valuable parameter in the differentiation of acute rejection and acute tubular necrosis.

Acute Kidney Injury↗
PubMed1979

[Evaluation of the effectiveness of ALG in clinical kidney transplantation in alternating studies].

The efficacy of ALG (horse serum against cultured human lymphocytes) in addition to a standard azathioprine and steroid regimen has been investigated in an alternating series of 70 renal transplantations in adults. Patient survival was almost identical in both groups. Transplant survival after 2 years was 64% in the ALG group as opposed to 48% in the group without ALG. In agreement with current literature, immunosuppression with ALG in high dosage seems to be justified.

Adult↗
PubMed1978

Atraumatic method of renal blood flow estimation by 133Xenoninhalation and its application to transplanted kidneys.

Non-invasive measurement of organ blood flow can be achieved by tracing the elimination patterns of 133Xe administered by inhalation. An adaption of the method for the estimation of renal blood flow was developed. The 133Xe is accumulated in the tissue by re-breathing for 1 min and the time course of its washout from the kidney is followed for 14 min thereafter. Normal values were determined in ten dogs and forty-five healthy human volunteers. In man they were similar to results obtained with 131I-hippuran clearance. When flow rates were between 250 and 600 ml/100 g/min the correlation coefficient was 0.84. Only in cases with high cortex flow rates (greater than 600 ml/100 g/min) did the inhalation method give values higher than those determined by the 131I-hippuran clearance. In dogs the results closely correlated with results obtained by direct intra arterial xenon injection (r = 0.96). The value of the inhalation method in routine examination of patients with kidney transplants and its suitability for the early detection of ongoing rejection is demonstrated.

Animals↗