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Biomedical subjects

K Urban

Publications and source records attributed to K Urban.

At least 55 records · Page 3Linked to original sources
PubMed1994

Fixation-dependent vimentin immunoreactivity of mono- and polyclonal antibodies in brain tissue of cattle, rabbits, rats and mice.

The immunohistochemical staining of vimentin in paraffin-embedded sections from adult cattle, rabbit, rat and mouse brain fixed in different fixatives (formaldehyde, methacarn, ethanol) was examined using two monoclonal antibodies and a polyclonal antiserum. In non-trypsinized formaldehyde-fixed tissue sections both monoclonal antibodies and the polyclonal antibodies failed to stain vimentin. Following trypsinization of formaldehyde-fixed sections of the four species the meninges, endothelial cells of blood vessels, ependymal cells and the stroma of the choroid plexus were labelled by the monoclonal and polyclonal antibodies used. Astrocytes and Bergmann glial fibers in pretrypsinized formaldehyde-fixed sections from cattle, rabbit and rat brain, however, showed only weak staining. Fixation of cattle and rat brain in methacarn markedly improved the vimentin immunoreactivity of astrocytes and Bergmann glial fibers. The best fixative for the preservation of immunoreactive determinants of vimentin in astrocytes and Bergmann glial fibers in cattle, rabbit and rat brain was ethanol. In brain tissue from mice both monoclonal antibodies labelled only mesoderm-derived tissue components, but did not recognize vimentin in astrocytes and Bergmann glial fibers. Pre-heating formaldehyde-fixed sections from cattle, rabbit and rat brain in a microwave oven prior to the immunohistochemical reaction resulted in an enormous enhancement of vimentin staining of mesoderm-derived tissues, of astrocytes and bergmann cell fibers.

Acetates↗
PubMed1993

[Initial clinical experience with BAS O, a bioactive glass-ceramic material].

The authors describe a group of patients where the surgical operation involved among others filling of bone defects with glass-ceramic material--dense (as granules) or porous (as a block). Glass-ceramics BAS O were developed in the laboratories of LASAK Co. in Prague. Two defects were of traumatic origin and osteosynthesis was part of three operations. The remaining defects were juvenile bone cysts, fibrous dysplasia and benign bone tumours. The follow-up period after operation varied in the first 11 patients between 6 months and 2 years. In patients of the mentioned group no problems of healing of the surgical wound were recorded nor allergic and side-reactions. The incorporation of glass-ceramic material was followed up by X-ray after three-months intervals. In no instance lighter areas were found on the X-ray pictures suggesting a fibrous outer layer. On the contrary, the trabeculae reached gradually its close vicinity. Based on experience from experimental work and investigation of X-ray signs of healing, the patients were allowed to burden the operated extremity after three months. The basic laboratory examinations made in these patients were within the normal range. In particular calcium throughout the investigation period in the normal range, the phosphorus levels varied near the upper borderline, alkaline phosphatase levels were in many young patients elevated and acid phosphatases varied. In eight patients during the postoperative period eosinophilia was revealed in the haemogram.

Adolescent↗
PubMed1990

[Ataralgesia and local anesthesia in translumbar aorto-arteriography].

The use of ionic contrast media for translumbar aortoarteriography frequently results in unwanted movements of lower extremities caused by transient convulsive muscular pains. The use of suitable pretreatment and examination procedure may help to avoid general anesthesia and the ensuing risk. The communication presents the procedure and drugs which enable making aortoarteriography without general anesthesia faster and without muscular pains in the extremities.

Analgesia↗
PubMed1989

[Myoblastic myoma of the tendon of long flexor of the thumb].

The case history of a female patient with flexion contracture of the thumb, having been caused by a tumour originated in the tendon of musculus flexor pollicis longus and histologically classified as a myoblastic myoma is presented. After a complete resection of the tumour and substitution of the originated defect of the tendon the function of the right hand became possible to a full extent and no signs of a relapse may be seen four years after the operation.

Adult↗
PubMed1989

[Possibilities of diagnosis and treatment of jumper's knee].

A total of 24 sportsmen having painful sensations upon apex patellae as well as in the course of ligamentum proprium patellae had been submitted to both examination and treatment. Initially, the conservative management of the disease had only partial benefit. More pronounced conditions characterized histologically by microruptures, fiber degeneration up to necrosis showed no response to this treatment. In such cases, the excision of affected ligamental portion with subsequent fascial strand implantation was indicated. Another compound of surgical approach was a deperiostage of patellar apex. From 12 cases managed by surgery, the excellent results were achieved in 9, good in 2 and bad in 1 patient, respectively.

Cumulative Trauma Disorders↗
PubMed1989

[Personal experience with the diagnosis and treatment of aneurysmal bone cysts].

Nine cases of aneurysmal bone cysts were diagnosed and treated at the Department of Orthopedics in Hradec Králové in the period of 1975 to 1986. Diagnosis was based on clinical examination, radiography and histological picture. Therapy included in two thirds of cases excochleation with the following stabilization of the involved bone, resections were carried out in remaining patients. One relapse occurred after excochleation with the character of a giant-cell tumour which evidenced a close relation to this disease.

Adolescent↗
PubMed1989

[Surface bioactive ceramics (preliminary report)].

The Research and Development Institute of Industrial Glass in the town of Teplice (North Bohemia) has developed bioceramics (BAS-O) with presupposed surface activity manifested during its implantation in the macroorganism. The test was carried out by means of Macor inert ceramics produced by Corning Co. In total there were performed 8 implantations of surface active bioceramics and 7 implantations of inert ceramics in the tibiae and femurs of four dogs. After two months the experimental animals were sacrificed and the implants were examined. All eight prisms of BAS-O surface active ceramics were firmly connected with the bone and could be removed from bones only by force. On the contrary, the control inert ceramics could be removed from the bone by a pincette. The contact X-ray pictures showed an interface of surrounding tissue around inert ceramics and contrary to this, in case of surface bioceramics the bone trabeculea penetrated into the implants. The surrounding fibrous tissue around inert ceramics was proved in all cases by microscopic examination. In case of surface active bioceramics the fibrous intermedial layer was not proved even by electron microscopy.

Animals↗
PubMed1989

[Partial rupture of the Achilles tendon in athletes].

The author discusses a group of ten sportsmen who suffered from achillodynia in the most distant part of the Achilles tendon, where the immediate cause was partial rupture. In seven instances the rupture was at the site of contact of the tendon with the prominent posterior part of the calcaneus. In the remaining three patients it was above the calcaneus. Two of these ruptures may have developed in conjunction with the application of a hydrocortisone preparation in the neighbourhood of the tendon. Treatment involved ablation of the prominent calcaneus, excision of the retrocalcanear bursa and suture of the tendon, depending on the extent of the defect. When the lesion was beyond contact with the calcaneus, the tendon was treated in the same way. The follow-up period of the patients is 7 months to 4 years. Nine sportsmen resumed their contest activities.

Achilles Tendon↗
PubMed1989

[The resection site in the treatment of giant cell bone tumors].

The authors discuss surgical procedures (resections and amputations) in a group of 35 patients with giant-cell bone tumours who were treated at the Orthopaedic Clinic in Hradec Králové. The diagnosis was established in cooperation by the orthopaedist, pathologist and roentgenologist. Resections were made in 24 patients, i.e. in 13 patients as primary operations, in 11 patients as secondary operations. The group comprised three tumours classified as malignant. After primary resection a relapse developed once, after secondary resection there were two relapses. Twenty-one patients, i.e. 87.5%, had no relapse. On account of two extensive relapses in soft tissues the affected extremity had to be amputated. One amputation was necessary on account of chronic osteomyelitis of the graft. The follow up period of the mentioned patients is between 3 and 26 years. The described investigation tested the possibility to extend indications of resection of giant-cell bone tumours also to conditions when the tumour perforates the affected skeleton and spreads to soft tissues in a favourable direction, i.e. muscles, without affecting important nerves and blood vessels.

Adolescent↗