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Biomedical subjects

K Uno

Publications and source records attributed to K Uno.

At least 217 records · Page 12Linked to original sources
PubMed1985

Ontogeny of 'macrophage' function. V. Differential effect of prostaglandin E2 on the activation of newborn and adult mouse macrophages.

Our previous papers indicate that peritoneal exudate macrophages (PM) of newborn mice are strongly suppressive for the tumor cell growth in comparison with those of adult mice. Neither newborn PM nor adult PM are cytolytic, but they both can be activated to be cytolytic by being cultured overnight either with a high concentration of bacterial lipopolysaccharide (LPS) or with a low concentration of LPS plus lymphokine. This paper shows that the activation of adult PM was inhibited by prostaglandin E2 (PGE2) added simultaneously with LPS, though that of newborn PM was not affected by PGE2. Adult PM, however, acquired the resistance to PGE2 by the preculture with LPS and/or lymphokine for 4 hr so as to be activated by LPS in the presence of PGE2. These results indicate that newborn PM are more activated than adult PM to a state which adult PM attain after moderate activation with LPS or lymphokine.

Age Factors↗
PubMed1985

Treatment of juvenile laryngeal papilloma with a combination of laser surgery and interferon.

The therapeutic results obtained from the treatment of juvenile laryngeal papillomas with combined laser surgery and alpha-type IFN treatment are reported. Three cases of papilloma occurring under four years of age were found to be of the fulminant type with a strong tendency for recurrence. The results were complete remission of papilloma in two cases and a residual trace in one case. Thus, at present, control of the disease has been successful. A combination of several modes of treatment may be necessary for a more lasting therapeutic effect. Currently, no single method has been used to treat the disease. Laser surgery and IFN are both powerful therapies for this tenacious disease.

Child, Preschool↗
PubMed1985

Direct and indirect effects of interferon on in vivo murine tumor cell growth.

We cloned two sublines (S1 and R1) of murine Meth A fibrosarcoma cells with respect to their sensitivity to a murine alpha/beta-interferon (IFN) preparation. The growth of S1 cells was suppressed and that of R1 cells was hardly affected by IFN in vitro. This was also the case with cells enclosed in cell-impermeable diffusion chambers in peritoneal cavities. Nevertheless, IFN suppressed the growth of not only S1 cells but also R1 cells in mice inoculated i.p. with these cells, and the survival rates of both S1 cell recipients and R1 cell recipients were markedly improved. S1 cells were observed microscopically to be injured by the direct effect of IFN in vitro and in vivo, but R1 cells in in vitro culture with IFN and those surviving in vivo in the presence of IFN appeared to proliferate well. In the peritoneal cavity of R1 recipients treated daily with IFN, the recruitment of macrophages was enhanced in comparison with untreated R1 recipients. Adherent peritoneal exudate cells obtained from IFN-treated, R1-bearing mice were highly suppressive for the in vitro growth of not only R1 cells but also allogeneic and human cells. The role of macrophages in the indirect effect of IFN on tumor cell growth is discussed.

Animals↗
PubMed1984

Improvement of chemical analysis of antibiotics. V. A simple method for the analysis of tetracyclines using reversed-phase high-performance liquid chromatography.

An isocratic high-performance liquid chromatographic method for the determination of tetracyclines is described using a mobile phase containing oxalic acid and C8- and C18-modified silica gel columns. For good separations of tetracyclines, oxalic acid concentrations of above 0.01 and 0.2 M respectively for parent tetracyclines (group I) and impurities in tetracycline (group II) are required. The optimum pH of the aqueous oxalic acids solution in the mobile phase is 2.0. The combinations of the C8-modified silica gel column with methanol-acetonitrile-0.01 M aqueous oxalic acid solution pH 2.0 (1:1.5:5) and the C18-modified silica gel column with methanol-acetonitrile-0.2 M aqueous oxalic acid solution pH 2.0(1:1:3.5) gave satisfactory results for groups I and II, respectively.

Acetonitriles↗
PubMed1984

Ontogeny of 'macrophage' function. IV. Newborn mouse macrophages strongly suppress tumour cell growth and readily acquire cytolytic activity in comparison with adult macrophages.

Peritoneal macrophages (PM) were prepared as adherent peritoneal exudate cells from newborn and adult mice injected i.p. with thioglycollate medium 4 days previously. In comparison with adult PM, newborn PM exerted a high suppressive effect on the in vitro growth of syngeneic and allogeneic tumour cells, though they did not manifest cytolytic activity. The high suppressive activity of newborn PM was maintained until about 2 weeks of age, and then declined rapidly until about 3 weeks of age toward the level of adult PM. The treatment with a high dose of LPS enhanced the suppressive effect of both newborn and adult PM, while a low dose of LPS was effective only for newborn PM. Necessary minimum dose of LPS to make PM significantly cytolytic was lower for newborn PM than for adult PM. Addition of a low concentration of LK to the culture for activating adult PM with LPS resulted in the augmentation of cytolytic activity and the reduction of necessary minimum dose of LPS. Activation of newborn PM by LPS was not affected by the addition of such a low concentration of LK. On the other hand, newborn PM were rapidly activated by LPS as compared with adult PM. LK accelerated the activation of adult PM by LPS. The activity of newborn PM to bind to tumour cells was higher than that of adult PM. These results seem to indicate that newborn PM are activated to some extent inherently or by some intrinsic agents.

Aging↗