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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,153 records · Page 64Linked to original sources
PubMed1987

Distribution of motoneurons involved in the prey-catching behavior in the Japanese toad, Bufo japonicus.

Coordinated activities of several muscles in the head region underlie the prey-catching behavior of anuran amphibians. As a step in elucidating the neural mechanisms generating these activity patterns in the Japanese toad, we labelled the motoneurons innervating 8 behaviorally relevant muscles using intramuscular (i.m.) injection technique of horseradish peroxidase (HRP), and examined their localization within the motor nuclei whose boundaries were determined by HRP application to the nerve trunk. All the motoneurons innervating the two jaw closer muscles (m. masseter major, m. temporalis) and m. submentalis were localized within the rostral subdivision of the trigeminal motor nucleus. The motoneurons innervating the only mouth opener muscle (m. depressor mandibulae) were scattered throughout the facial motor nucleus. The motoneurons innervating tongue (m. hypoglossus, m. genioglossus) and hyoid muscles (m. sternohyoideus, m. geniohyoideus) appeared within the hypoglossal nucleus with distribution patterns characteristic of the target muscles. Thus, we have revealed the neuroanatomical organization of the motoneurons relevant to the prey-catching behavior.

Animals↗
PubMed1987

Neurotensin-like immunoreactivity (NTLI) concentration in the cerebrospinal fluid of children and its alteration in a febrile aseptic meningitis.

Neurotensin-like immunoreactivity (NTLI) concentrations in the cerebrospinal fluid (CSF) of normal children and patients with febrile aseptic meningitis, aged 7 months to 15 years, were studied. The NTLI concentrations in CSF of 27 children with normal CSF findings were 160.1 +/- 54.6 pg/ml (mean +/- S.D.). The NTLI concentration in CSF of 26 patients in an acute phase of aseptic meningitis was 110.6 +/- 51.1 pg/ml which was significantly (P less than 0.01) lower than the controls. These patients had a mean temperature of 101.4 +/- 1.5 degrees F which remained elevated for an average of 3.5 days. The NTLI concentrations in CSF of 23 patients in a recovery phase (after blood and CSF findings became normal with no fever) were 166.5 +/- 57.8 pg/ml, which did not differ significantly from the normal. There were no statistical correlations between the NTLI concentration in CSF and the protein concentration or total cell count in CSF. These results suggest that NTLI concentration changes during a febrile aseptic meningitis and that it may be associated with thermoregulation.

Adolescent↗
PubMed1987

Development of host-dependent high-grade tumor-specific immunity through a novel mechanism triggered by the Lyt-2+ tumor-specific T cell clone (K7L) that induces temporal growth of L1210 leukemia-K7L-variant.

When a murine leukemia L1210-specific Lyt-2+ T cell clone, K7L, was injected i.p. into CD2F1 mice together with L1210, the normal growth of L1210 in the peritoneal cavity of the mice at the early stage (days 0 to 5) was strongly inhibited, but L1210 grew progressively at the middle-stage (days 5 to 10), and then was rejected at the late stage (days 10 to 20). The mice thus survived for long times (more than 60 days), whereas the normal control injected with L1210 alone died within 14 days. The L1210 that grew at the middle stage in mice initially inoculated with L1210 together with K7L was a K7L-insensitive (K7L-) variant. All of eight tumor clones established from L1210-K7L- by limiting dilution was insensitive to the antitumor activity of K7L, and this property of tumor clones was stable after repeated in vitro passage. The initial depression of the L1210 growth by K7L followed by growth and rejection of the variant L1210-K7L- by the host T cell activity was then found to prepare a strong, long-lasting (more than 3 mo) immunity to protect mice against the high-dose (10(7) cells per mouse) challenge of original L1210. Corresponding to this result, definite tumor (L1210)-specific cytotoxic T lymphocyte (CTL) activity against both variant and original L1210 targets was developed by antigen (L1210) restimulation in the culture of spleen cells from these mice, but was not increased to a detectable level before L1210-K7L- variant started to grow. It was suggested that the 1210-K7L- variant and the original L1210 should have the common tumor-specific antigen that was independent of the K7L-reactive antigen, and that original L1210, whose growth was retarded by K7L, primed the host with the common antigen to be enormously boosted by the subsequently growing L1210-K7L- variant.

Animals↗
PubMed1987

The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation.

Multidrug resistance in human KB carcinoma cells selected for resistance to colchicine, vinblastine, or adriamycin results from overexpression, and frequently amplification, of a specific gene (mdr1). Overlapping cDNA clones representing a complete 4.7-kilobase mdr1 transcript have been obtained from multidrug-resistant KB cells. Primer extension and S1 nuclease protection experiments show that two transcripts initiate 136 and 140 bases upstream from the first ATG codon in all human multidrug-resistant cell lines. The mdr1 gene is expressed in human normal kidney cells and HepG2 liver cells as a poly(A)+ RNA which starts from the same sites. Less prominent transcripts were found to initiate 155-180 bases upstream from the first ATG codon in vinblastine- or adriamycin-selected cell lines and 480-630 bases upstream in colchicine-selected cell lines. Southern hybridization analyses with different portions of a full-length cDNA indicate that the human mdr1 gene encompasses at least 70 kilobases of DNA amplified in all highly multidrug-resistant cell lines.

Base Sequence↗
PubMed1987

An improved method for correlative light and electron microscopic examination of cobaltic-lysine-labelled neurons.

We describe an improved method for correlative light and electron microscopic examination of synaptic organization of neurons extracellularly labelled by the axonal transport of cobaltic-lysine (Co-lys). After filling the neurons with Co-lys and precipitating the CoS, the brain is fixed in a double-aldehyde fixative, and thick slices are cut using a Microslicer. The slices are intensified using a physical developer, postfixed in OsO4 and embedded in resin. By cutting alternating semithin and ultrathin sections, it is possible to specify sites and types of synaptic contacts on labelled neuronal profiles.

Animals↗
PubMed1987

Morphology and distribution of the motor neurons of the accessory nerve (nXI) in the Japanese toad: a cobaltic lysine study.

Motoneurons supplying the accessory nerve (nXI) of the Japanese toad were retrogradely labelled by applying the cobaltic lysine to the cut end of the nerve. They had morphological characteristics and a distribution pattern similar to those of the rostral spinal motoneurons rather than the branchial motoneurons. We propose that the anuran nXI is equivalent to the so-called spinal portion of the nXI of other vertebrates, and both should be regarded as part of the rostral spinal nerves rather than the nerve accessory to the vagus nerve.

Accessory Nerve↗
PubMed1987

Morphology and distribution of the preganglionic parasympathetic neurons of the facial, glossopharyngeal and vagus nerves in the Japanese toad: a cobaltic lysine study.

We labelled the preganglionic parasympathetic neurons of the facial, glossopharyngeal and vagus nerves of the Japanese toad by applying the cobaltic lysine to the cut end of the respective nerve, and examined their morphology and distribution. These neurons form an almost continuous cell column consisting of small neurons with less elaborate dendrites, and occupy a more dorsal position than the motoneurons of the corresponding nerves. The results suggest the presence of the amphibian homologues of the salivatory nucleus and the dorsal motor nucleus of the vagus.

Animals↗
PubMed1987

Hemagglutination inhibition antibodies in congenital rubella syndrome. A 17-year follow-up in the Ryukyu Islands.

We studied long-term rubella hemagglutination inhibition (HI) antibody titers in 381 children born between 1965 and 1966 with congenital rubella syndrome and in 344 of their mothers, who had had postnatally acquired rubella infection. Serum specimens were obtained 12 times during a 17-year period. The initial geometric mean titer of the rubella HI antibody was higher in the children than in their mothers, but the titer declined more rapidly in the children. After 17 years it was 2(5.4) in the children and 2(6.9) in the mothers. In 13 (3.4%) of the children, the rubella HI antibody titer reverted to negativity after three to 17 years. None of the mothers showed a reversion.

Adolescent↗
PubMed1987

Human T-cell lymphotropic virus type I infection in neonates.

One hundred twenty-eight serum samples, collected from infants transfused at the neonatal care unit of Kyushu University Hospital, Fukuoka, Japan, were tested for the presence of antibody to human T-cell lymphotropic virus type I (HTLV-I) by the enzyme-linked immunosorbent assay and the indirect immunofluorescence test. Three of 128 were positive for anti-HTLV-I antibody. The three seropositive patients had received 62, 82, and 1160 mL of transfused blood. These patients did not receive any transfusion thereafter. In addition, no anti-HTLV-I antibodies were detected in the sera of their mothers and siblings. These data suggested that HTLV-I infection is one of the transfusion-associated complications in neonates.

Antibodies, Viral↗
PubMed1987

Malignant histiocytosis in infants: surface marker analysis of malignant cells in two cases.

Two infants with malignant histiocytosis, as diagnosed by morphological and cytochemical examinations of malignant cells infiltrating pleural effusion, skin, and bone marrow, are described. Surface marker analysis of the malignant cells with monoclonal antibodies showed these cells to express markers of T-lymphocytes and HLA-DR antigen, but not those of B-lymphocytes, granulocytes, or monocytes. These immunological findings demonstrated that the neoplastic cells in malignant histiocytosis of infancy may be of T-cell origin, rather than histiocytic or monocytic origin.

Antibodies, Monoclonal↗
PubMed1987

Sperm chromosome analysis as a new system to test human X- and Y-sperm separation.

The correlation between the percentage of F-body-positive spermatozoa (F-sperm) and that of Y-chromosome-bearing spermatozoa (Y-sperm) determined by human sperm chromosome analysis was examined after spermatozoa were separated by an albumin column method [Ericsson et al, Nature 246:421-424:1973]. The percentages of F-sperm were 43.1 +/- 4.8 (36.1-48.8) and 45.8 +/- 8.3 (35.3-66.5) before and after separation, respectively. A slight but significant (p less than 0.05) increase in F-sperm after the separation was observed in two semen donors. A total of 570 sperm karyotypes was analyzed and the percentage of Y-sperm ranged from 36.0 to 59.1 with a mean of 47.3. In one semen donor, the percentage of Y-sperm (45.6) was significantly (p less than 0.05) higher than that of F-sperm (35.3) after separation. In the remaining nine semen donors, the results obtained by chromosome analysis corresponded well with those obtained by F-body scoring. The validity of the albumin column method in isolating a Y-sperm-rich sperm population is discussed.

Animals↗
PubMed1987

Facial palsy in Kawasaki disease. Report of two cases and a review.

A case of facial palsy was reported initially in 1974 by Murayama as one of the neurological manifestations in Kawasaki disease. Thereafter, an additional nine case have been documented in Japan. This facial palsy, in the revised "Diagnostic Guideline of Kawasaki Disease" released in 1984, has been added recently as one of the neurological signs and symptoms of Kawasaki disease. This is a report on two cases of Kawasaki disease showing facial palsy with indurative oedema during their clinical course, and also a clinical review of the ten previously reported cases of facial palsy complicating Kawasaki disease.

Aspirin↗