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Biomedical subjects

K Tsuji

Publications and source records attributed to K Tsuji.

At least 757 records · Page 42Linked to original sources
PubMed1981

Acute irreversible rejection after withdrawal of azathioprine in renal allograft recipients.

Azathioprine was withdrawn from five recipients after renal transplantation because of hepatic damage. In three out of five cases, clinical courses were uneventful for more than 1 year after withdrawal (Group A). In the other two cases, rejection episodes occurred 3 and 5 months after withdrawal (Group B). Differences in histocompatibility, the interval between the operation and withdrawal, and the maintenance dosis of prednisolone were not practically striking in either group, but rejection episodes occurred early in group B. We evaluated the results and considered the mechanisms of tolerance after withdrawal of azathioprine because of hepatic damage in renal allograft recipients.

Adult↗
PubMed1981

Role of macrophages in T lymphocyte response to Candida allergen in man with special reference to HLA-D and DR.

Activation of naturally sensitized human T lymphocytes to Candida allergen was studied using three HLA-D and DR heterozygote Japanese cells (Dw1.DR1/DYT.DR4, Dw12.DR2/DYT.DR4, DYT.DR4/DEn.DR blank) and four HLA-D and DR homozygote cells (Dw1.DR1, Dw12.DR2, DYT.DR4, DEn.DR blank). In vitro activation of T lymphocytes to Candida allergen was found to require the presence of autologous or allogeneic compatible HLA-Dw1.DR1 and Dw12.DR2 macrophages.

Antigens, Fungal↗
PubMed1981

[Tricuspid valve motion and tricuspid valve ring size in normals and patients with atrial septal defect (author's transl)].

To investigate the normal configuration of the tricuspid valve and the normal size of the tricuspid valve ring, 17 normals, 20 patients with atrial septal defect, 4 patients with tricuspid valve prolapse, and 41 patients with various diseased conditions were studied by cross-sectional echocardiography. The tricuspid valve ring diameter in atrial septal defect was judged to be increased in comparison with that of normals. All the patients with tricuspid valve prolapse exhibited an excessive systolic ballooning of the leaflets toward the right atrium. However, a systolic ballooning of the leaflets alone was frequently observed in normals and many other conditions. Therefore, we conclude that cross-sectional echocardiography has some clinical limitations in the differential diagnosis between tricuspid valve prolapse and normals.

Adult↗
PubMed1981

Increased risk of malignant transformation of hydatidiform moles in older gravidas: a cytogenetic study.

Cytogenetic studies were performed in 35 cases of complete hydatidiform mole, and the results were correlated with maternal age and other clinical parameters. The rate of occurrence of aneuploid cells in the 18- to 39-year age group was 19% of the total cells; the rate in the 43- to 52-year age group was 25%. The rate of aneuploid cells between 2n and 3n was 20% in the latter group but only 7% in the former group. Aneuploid cells were not found in normal villi but were found to predominate in invasive mole and choriocarcinoma. These results suggest that hydatidiform mole may have a greater potential for malignant transformation in patients 40 years and older than in younger patients.

Adult↗
PubMed1981

[The ultrastructure of a gangliocytoma (author's transl)].

The tumor tissue removed surgically from the left parietal subcortical area of a 16-year-old boy was studied by light and electron microscopy. Our results were identical to cases reported previously. By light microscopy, the neoplasm was composed mostly of mature and immature ganglion cells and glial cells. Ganglion cells with large nuclei and prominent nucleoli had characteristic Nissl substance in various amounts and they were encompassed in a network of fibrous connective tissue. Glial cells were mainly oligodendrocytic. Astrocytes were small in number and did not show neoplastic growth. By electron microscopy, mature ganglion cells contained dense core vesicles in their cytoplasm and processes. The size of the vesicles ranged from 100 to 200 nm. Furthermore ganglion cells were surrounded by their own processes and also processes of glial cells. Ganglion cells were rarely found in synaptic contact with adjacent processes. Instead, oligodendrocytes were sometimes situated adjacent to these ganglion cells. These findings suggest that the tumor under study may be of hamartomatous origin. Aside from mature ganglion cells and glial cells, immature cells were present. From viewing the architecture of these immature cells, we concluded that they were also ganglion cells.

Adolescent↗
PubMed1980

Gm allotypes in myasthenia gravis.

Gm typing and acetylcholine receptor antibody assay were performed on serum samples from 74 patients with myasthenia gravis (31 male, 43 female) and from 236 unrelated normal blood-donors. The haplotype Gm1,2,21 was significantly more common in patients with myasthenia gravis (relative risk = 3.24), especially those with thymoma (relative risk = 6.99). The frequency of haplotype Gm1,2,21 was further increased in patients with severe generalised myasthenia gravis (relative risk = 10.52). The frequency of Gm1,2,21 was also increased in patients with high acetylcholine receptor antibody titres (greater than 5 pmol/ml). The results indicate the presence of a pathogenic gene close to the IgG heavy-chain gene complex in the 6th chromosome in the patients with myasthenia gravis, especially those with thymoma.

Adolescent↗
PubMed1980

Formation of mutagens by sorbic acid-nitrite reaction: effects of reaction conditions on biological activities.

Conditions of the reaction between sorbic acid and sodium nitrite generating mutagenic principles were examined. In the rec-assay and the Ames reversion assay, the maximal mutagenic activity was obtained in a pH range of 3.5-4.2. Mutagenic and growth-inhibitor activities of five C-nitro and C-nitroso compounds were studied. The product Y, 2-methyl-1,4-dinitropyrrole, was the strongest mutagen among them.

Drug Interactions↗
PubMed1980

HLA-linked genetic control in natural rubella infection.

HLA antigen frequencies were investigated in 115 patients with congenital rubella syndrome and 93 of their mothers in the Ryukyu Islands, Japan. A high frequency of HLA-B15 and a low frequency of HLA-Bw22 were found in the mothers. HLA-B15 was associated with high antibody responsiveness to rubella vaccine in our previous study, whereas HLA-Bw22 was one of the HLA antigens which showed low antibody responsiveness. There was no association between HLA antigens and viral replication in rubella vaccine recipients in the present study. These results indicate that and HLA-linked genetic background in the mothers is related to susceptibility to congenital rubella syndrome, and that the mechanism of the relation may be mediated via immune response genes.

Adolescent↗
PubMed1980

Limulus amoebocyte lysate assay for detection and quantitation of endotoxin in a small-volume parenteral product.

A Limulus amoebocyte lysate gel-clotting method for the determination of endotoxin in a small-volume parenteral product has been described. Sample dilution with 0.1 M potassium phosphate monobasic buffer (pH 8.0) effectively eliminated assay interference, whereas dilution with water did not. The threshold pyrogenic dose for Escherichia coli EC-2 and O127:B8 endotoxins was determined to be 1.0 ng of endotoxin per kg of body weight. Not more than 1.0 ng of endotoxin (the threshold pyrogenic dose) per the highest recommended human dose or the USP pyrogen test dose per kg of body weight, whichever dose is more stringent, is a logical limit for the quantity of bacterial endotoxin in small-volume parenteral products. Excellent correlation was attained when this criterion was used to compare the Limulus amoebocyte lysate assay with the USP pyrogen test.

Animals↗
PubMed1980

Quantitative analysis of cholesterol in foods by gas-liquid chromatography.

In order to develop a simple and exact analytical method for cholesterol determination in foods by gas-liquid chromatography, several experiments were carried out in collaboration with several universities and institutes. For the extraction of lipids from foods, it was decided that chloroform-methanol (2:1) extraction was the most suitable procedure. Since pretreatment procedures using methods such as thin-layer chromatography and florisil column chromatography to purify the unsaponifiable matters reduced the recovery of cholesterol, and good results were obtained without applying the pretreatment, these procedures were concluded to be unnecessary. Gas chromatograms obtained with free sterol showed results similar to those of the trimethylsilyl ether derivative and acetate. 5-alpha-Cholestane is used as a good internal standard.

Chloroform↗