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Biomedical subjects

K Tateishi

Publications and source records attributed to K Tateishi.

At least 127 records · Page 7Linked to original sources
PubMed1984

Distribution, origin, and fine structures of cholecystokinin-8-like immunoreactive terminals in the nucleus ventromedialis hypothalami of the rat.

The distribution, origin, and fine structures of cholecystokinin-8-like immunoreactive ( CCKI ) fibers in the nucleus ventromedialis hypothalami (vm) of the rat were examined using immunohistochemistry. CCKI fibers were moderately concentrated in the ventrolateral part of the vm, decreasing in number dorsomedially. However, no CCKI cells were seen in the vm even in colchicine-treated rats. In addition, the destruction of the lateral part of the nucleus parabrachialis dorsalis (pbd) where numerous CCKI cells were seen resulted in a marked decrease in CCKI fibers in the vm on the operated side. Electron microscopic observations revealed that CCKI terminals make synaptic contact with soma, spine, and the proximal segment of the dendrite. The neurons making synaptic contact with the CCKI fibers had moderately electron-lucent cytoplasm. These findings suggest that the CCKI fibers in the vm originate from CCKI cells in the lateral part of the pbd and directly influence the vm neurons.

Animals↗
PubMed1983

Ontogeny of cholecystokinin-8-containing neuron system of the rat: an immunohistochemical analysis. I. Forebrain and upper brainstem.

Ontogeny of the cholecystokinin-8 (CCK) neuron system in the forebrain and upper brainstem of the rat was investigated by means of indirect immunofluorescence. CCK cells and fibers first appeared in the developing ventral tegmental area and in the primordium of the medial forebrain bundle, respectively, at gestational day 15 (12-14-mm embryos). From that time, CCK cells appeared in various areas of the forebrain and upper brainstem until birth and reached the maximum content at postnatal day 10. After postnatal day 10, although CCK cells tended to decrease slightly in number, colchicine treatment in the adult rats brought out numerous CCK cells in the same areas. In contrast, although CCK fibers developed only slightly during the fetal period, marked development was seen after birth, particularly between postnatal days 5 and 10. After that time, as the rats grew, CCK fibers continued to increase in number and formed a meshwork of varying density in various areas of the forebrain and upper brainstem.

Animals↗
PubMed1983

Ontogeny of cholecystokinin-8 containing neuron system of the rat: an immunohistochemical analysis--II. Lower brain stem.

The ontogeny of the cholecystokinin-8 neuron system in the lower brain stem of the rat was investigated by means of indirect immunofluorescence. Cholecystokinin-8-like immunoreactive structures first appeared in the primordium of the ventral tegmental area, ventral tegmental nucleus of Gudden and the area lateral to the superior central nucleus of the fetus on gestational day 17. Although cholecystokinin-8-like immunoreactive structures appeared gradually in various lower brain stem areas from this day, and developed markedly from postnatal day 6, thereafter, the ontogeny of cells and fibers was different. In general, the fibers increased in number as the rats grew and reached the maximum in adulthood, whereas the cells reached a maximum number between postnatal days 6-12, after which they decreased in number. Colchicine treatment, however, demonstrated the presence of cholecystokinin-8-like immunoreactive cells in various areas of the lower brain stem. These findings suggest that the cholecystokinin-containing neuron system is active in the adult and that cholecystokinin-containing cell somata cannot store a sufficient amount of the peptide for visualization in the absence of colchicine. Thus, it might be concluded that cholecystokinin may play an important role in the brain function of the adult as a neurotransmitter or neuromodulator, though its exact function is now open to discussion.

Animals↗
PubMed1983

The distribution of cholecystokinin octapeptide-like structures in the lower brain stem of the rat: an immunohistochemical analysis.

The distribution of immunoreactive cholecystokininoctapeptide (CCK-8)-like structures in the lower brain stem of the rat was investigated using indirect immunofluorescence. In addition to the well known immunoreactive CCK-8-like containing cell groups such as those in the ventral tegmental area, substantia grisea centralis of the mesencephalon, and n. linealis rostralis, the present study demonstrated a much wider distribution of immunoreactive CCK-8-like cells in the lower brain stem, i.e. those in the inferior colliculus, n. parabrachialis colliculi posterioris, lateral lemniscus, lateral parabrachial area, n. centralis superior, nucleus of group O, pontine substantia grisea centralis, n. tractus solitarii, area postrema, n. tractus spinalis nervi trigemini and reticular formation just dorsal to the inferior olivary complex. We also demonstrated an extensive network of immunoreactive CCK-8-like fibers in various areas of the lower brain stem, including the auditory system, visual system, viscerosensory area, parabrachial nucleus, dorsal and ventral tegmental nuclei, and interpeduncular nucleus. The possible importance of CCK is briefly discussed.

Animals↗
PubMed1983

Plasma cholecystokinin responses after ingestion of liquid meal and intraduodenal infusion of fat, amino acids, or hydrochloric acid in man: analysis with region specific radioimmunoassay.

A highly sensitive and precise radioimmunoassay system for plasma cholecystokinin (CCK) was developed with the anti-CCK-8 specific antiserum which raised against N-terminal amino acids residue of sulfated CCK-8 and reacted with CCK-8, CCK-33, and CCK-39 but not with gastrin and its related peptides. Mean concentration of the fasting plasma CCK determined with this method using CCK-8 as standard was 12.9 +/- 5.9 pg/ml in normal subjects (n = 26), and in patients with hepatic cirrhosis it was significantly higher (36.7 +/- 16.9 pg/ml, n = 9, p less than 0.01) than in normal subjects. In six young healthy volunteers, intraduodenal infusion of fat caused a significant increase (p less than 0.05) of plasma CCK from a basal level of 8.0 pg/ml to a peak of 43.0 +/- 12.0 pg/ml at 20 min after starting of infusion. In the same subjects, a significant increase of plasma CCK was also observed by amino acids infusion, but no elevation of plasma CCK level was found during intraduodenal acidification.

Adult↗
PubMed1982

Production of rabbit antibody specific for amino-terminal residues of cholecystokinin octapeptide (CCK-8) by selective suppression of cross-reactive antibody response.

Antibody specific for the amino-terminal region of cholecystokinin octapeptide (CCK-8) was generated in a highly reproducible way in New Zealand white rabbits by a novel immunization procedure which involves immunization with CCK-8 peptide conjugate coupled with keyhole limpet hemocyanin (KLH) and inhibiting cross-reacting antibody formation by treatment of the animals with a potent tolerogenic conjugate of beta-alanyl-tetragastrin and a copolymer of D-glutamic acid and D-lysine (D-GL). The antisera thus produced specifically react with an amino-terminal region of CCK-8 but not with the non-sulfate form of CCK-8, nor with the carboxy-terminal region which shares a cross-reactive determinant among gastrin and cholecystokinin-related peptides (caerulein, CCK-4, CCK-8, CCK-33 and CCK-39). The antisera produced by this method allowed us to measure specifically CCK in extracts from tissue such as duodenum containing gastrin and CCK at comparable levels.

Amino Acids↗
PubMed1982

Proliferative pattern of seminal vesicle cells and the production of testosterone and 5 alpha-androgens from birth to adulthood in mice.

Proliferation of seminal vesicle cells and the concentration of testicular and serum androgens (testosterone and 5 alpha-androgens) from birth to adulthood were investigated in mice. The weight of the seminal vesicles increased significantly from day 0 to day 10 after birth (approximately 0.1-1 mg), remained nearly constant in the next 10 days, and increased again (1-50 mg) thereafter. As an index of cell proliferation, two distinct peaks of 5-[125I]iodo-2'-deoxyuridine ([125I]IdUrd) uptake by the whole seminal vesicles were found at days 8 and 30. Types of proliferating cells examined by autoradiography after injection of [3H]thymidine were partially testis-dependent fibromuscular and epithelial cells in the first peak and largely testis-dependent epithelial cells in the second peak. The [125I]IdUrd uptake values found around day 20 and after day 40 were similar to those in neonatally castrated mice. Concentrations of testicular and serum androgens were relatively high on days 8, 30, 35, 40, and 60 (0.2-0.7 ng/mg tissue and 1.3-2.2 ng/ml, respectively) but were very low on day 18 (0.02 ng/mg tissue and 0.4 ng/ml). These findings lead to the hypothesis that androgens secreted from neonatal and prepubertal mouse testes play a major role in the proliferation of seminal vesicle cells and that the quiescent interval of androgen secretion and action occurs around the 20th day after birth in mice.

Aging↗
PubMed1981

A novel immunization procedure for production of anti-cholecystokinin-specific antiserum of low cross-reactivity.

An anti-cholecystokinin octapeptide (CCK-8) antibody without cross-reactivity with pentagastrin, whose amino acid sequence is identical with the carboxy-terminal portion of CCK-8, was produced by immunization with CCK-8-keyhole limpet hemocyanin and injection of a conjugate of pentagastrin and a copolymer of D-glutamic acid and D-lysine to inhibit production of antibody cross-reacting with pentagastrin.

Animals↗
PubMed1980

A novel immunization procedure involving pretreatment with cross-reacting steroid conjugated to a copolymer of D-glutamic acid and D-lysine for production of low cross-reactive antisera.

Anti-testosterone and anti-5 alpha-dihydrotestosterone antisera have been produced by pretreatment of mice with a cross-reacting steroid coupled to a copolymer of D-glutamic acid and D-lysine to inhibit production of cross-reacting antibodies before immunization with the specific steroid conjugated to a protein. 15 beta-Carboxyethylmercapto derivatives were used as haptens. The cross-reactivities of the anti-testosterone and anti-5 alpha-dihydrotestosterone antisera with 5 alpha-dihydrotestosterone and with testosterone were 0.49 and 13.5%, respectively.

Animals↗
PubMed1980

Noninvasive transcutaneous oxygen pressure diagnosis of reversed ductal shunts in cyanotic heart disease.

Double-site transcutaneous oxygen pressure (tcPO2) monitoring was carried out in 24 infants with cyanotic heart disease and in 12 acyanotic "control" infants. The tcPO2 values were recorded continuously as ambient forced inspiratory oxygen (FIO2) was varied from 0.21 to almost 1.0. The recordings obtained in the cyanotic infants could be grouped in one of four patterns, each of which correlated closely with the altered hemodynamic characteristics of heart disease. These data confirm the value of this noninvasive technique for hemodynamic and anatomic diagnosis of cyanotic heart disease, especially in cases with arotic arch anomalies with reversed ductal shunts.

Heart Defects, Congenital↗