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Biomedical subjects

K Tashiro

Publications and source records attributed to K Tashiro.

At least 541 records · Page 30Linked to original sources
PubMed1985

[Two cases of giant fusiform aneurysm presenting ischemic attacks].

It is said that major clinical sign of giant fusiform aneurysm is so-called mass sign compressing surrounding tissues and ischemic attack caused by the aneurysm is very rare. The authors experienced such uncommon two patients with partially thrombosed fusiform aneurysm who developed ischemic attack. Case 1: A 68-year-old man was admitted to our hospital in semicomatous state with ocular bobbing and decorticate posture of sudden onset. Enhanced CT scans taken at admission showed a presence of homogenously enhanced mass continuing to bilateral posterior cerebral arteries, which compressed the pons from right ventro-lateral side (Fig. 1 a,b). Plain CT scan taken 10 days after admission demonstrated extensive low density area in the pons (Fig. 1c). Basilar artery and P1 segment of the right posterior cerebral artery are ectatic, tortuous and elongated (Fig. 2a). The ectatic basilar artery was displaced upward and posteriorly (Fig. 2b). He developed locked-in syndrome and died of pneumonia six months after admission. At autopsy, large aneurysm measuring 3.5 X 2.0 X 1.9 cm was verified in the basilar artery (Fig. 3a). Cross section of the basilar artery aneurysm showed that laminated thrombosis nearly occupied the lumen with partially unobstructed portion (Fig. 3b). Also, horizontal section of the pons demonstrated a presence of old infarction located in the perforating arteries territory (Fig. 3c). Case 2: A 42-year-old man was initially hospitalized in 1978 because of multiple intracranial meningiomas and subtotal removal of meningiomas located in the tuberculum sellae and the foramen magnum was performed.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1985

Timing of the initiation of rRNA gene expression and nucleolar formation in cleavage embryos arrested by cytochalasin B and podophyllotoxin and in cytoplasm-extracted embryos of Xenopus laevis.

The timing mechanism of rRNA gene expression was studied in Xenopus laevis embryos whose cleavage was arrested by treatment with cytochalasin B or podophyllotoxin, and in fertilized eggs from which about 40% of the cytoplasm was extracted by micromanipulation. rRNA synthesis was activated in the cleavage-arrested embryos and also in cytoplasm-extracted embryos at the time when the control embryos commenced synthesizing rRNA at the late blastula stage. Formation of the nucleolar structure occurred at the normal time in the nuclei of cleavage embryos arrested by podophyllotoxin. Therefore, neither twelve continuous cell divisions nor the nuclear division which normally precedes the MBT (mid-blastula transition) was the factor determining the timing of rDNA expression. Moreover, lowering of the cytoplasmic:nuclear ratio to a certain threshold level was not a prerequisite for rDNA activation. Some regulatory mechanism dependent on the time elapsed from fertilization is likely to be the important factor permitting rDNA expression.

Animals↗
PubMed1984

The appearance of eosinophil-directed chemotactic inhibitory factor in the serum of complete Freund's adjuvant-treated guinea pigs.

When guinea pigs were treated by complete Freund's adjuvant (CFA), eosinophil-directed chemotactic inhibitory factor (ECIF) appeared in the serum of the treated animals. The inhibition of the response of eosinophils by ECIF was selective for the T cell-derived chemotactic factor, which has been isolated from DNP-ascaris extract-induced skin lesions (termed delayed ECF-a) but not for another ECF (termed delayed ECF-b), which was also isolated from the same skin lesions. ECIF was detected in the serum after a single CFA injection, and no significant increase of ECIF activity was observed in the serum of animals injected with CFA twice. The ECIF activity in the serum was associated with fractions of MW 70,000 and 12,500, and failed to bind to Con A-Sepharose. Furthermore, ECIF was absorbed by eosinophils but not by macrophages suggesting that eosinophils have receptor sites for ECIF. On the other hand, chemotactic inhibitor against delayed ECF-b was not detected in the serum. It was thus suggested that inhibition of eosinophil reaction by CFA treatment is related to ECIF, inhibition being selective for the eosinophil response to delayed ECF-a.

Adjuvants, Immunologic↗
PubMed1984

Development of a highly sensitive enzyme-immunoassay for serum carbonic anhydrase-III.

A highly sensitive sandwich enzyme-immunoassay (EIA) for human muscle carbonic anhydrase isozyme III (CA-III) has been developed using microplate as a solid-phase and peroxidase as a labelled enzyme. The assay can detect levels as low as 2 ng/ml when 20 microliter of sample sera were used. Sera from patients with various neurological diseases were studied using this method, and elevated serum CA-III levels were found in patients with Duchenne muscular dystrophy, limb-girdle dystrophy, fascioscapulohumeral dystrophy, polymyositis and amyotrophic lateral sclerosis. The values correlated well with the results of radioimmunoassay (RIA), with a correlation coefficient of 0.92 (P less than 0.001). We feel EIA is preferable to RIA for its simple methodology.

Carbonic Anhydrases↗
PubMed1984

[Case of acoustic neurinoma with intratumoral hemorrhage extending to cerebellar hemisphere].

A case of acoustic neurinoma which developed intratumoral hemorrhage extending to the right cerebellar hemisphere, is reported. A 54-year-old man was diagnosed as cerebello-pontine angle tumor, possibly acoustic neurinoma, from typical clinical course, neurological signs and findings of skull X-P and CT scan at the first visit to our out-patient clinic on July 28, 1981. On August 6, 1981, he was transferred to our in-patient ward in emergency because of sudden onset of headache and impairment of consciousness. At emergency admission, he was found to be comatous and CT scan revealed round-shaped high density in the right cerebellar hemisphere, continuing from the right cerebello-pontine angle tumor with enlarged lateral ventricles, which indicated occurrence of intratumoral hemorrhage. At emergency operation, hematoma in the right cerebellar hemisphere was evacuated and the tumor in the right cerebello-pontine angle was partially resected. Microscopically, the tumor was composed of compact, finely fibrillated cells of Antoni A tissues, and loose patterns of Antoni B tissue, being diagnosed as schwannoma, and also abnormal vascular proliferations were identified just adjacent to foci of hemorrhage noted within tumor tissues. The pattern of abnormal vascular proliferations within this tumor were almost identical with telangiectasia. The authors concluded that intratumoral hemorrhage in this case occurred most likely due to disruption of histologically verified abnormal vascular proliferations.

Cerebellopontine Angle↗
PubMed1984

The regulation of tissue eosinophil chemotactic factor and inhibitor in allergic skin lesions of Freund's complete adjuvant-treated guinea-pigs.

The regulation of tissue eosinophilia induced by dinitrophenyl-ascaris extract (DNP-As) was investigated in guinea-pigs. Biphasic tissue eosinophilia peaking at 6 and 24 hr was observed in the skin lesions in Bordetella pertussis vaccine (Bp)-treated animals. In contrast, only the early phase of tissue eosinophilia was observed in Freund's complete adjuvant (FCA)-treated animals. Although less eosinophil chemotactic activity was detected in 24-hr-old skin extract of FCA-treated animals (FCA-extract), evident activity was recovered in the concanavalin A eluate (Con A-eluate) when FCA-extract was fractionated by Con A Sepharose. The chemotactic factor in Con A-eluate of FCA-extract was confirmed to be the T cell-derived eosinophil chemotactic factor, termed Delayed ECF-a, which has been isolated from allergic skin lesions by immunoadsorption. Another factor from the same skin lesions, Delayed ECF-b (which is a serum-derived one), was not detected in the FCA-extract. When eosinophils were mixed or pretreated with Con A-effluent of FCA-extract, the treated cells failed to be attracted by Delayed ECF-a, while the response to Delayed ECF-b was not affected, indicating that the inhibition was selective for Delayed ECF-a but not for Delayed ECF-b, and the eosinophil chemotactic inhibitory factor (ECIF) acts on eosinophils directly. Major ECIF activity was associated with a mol. wt. of 70,000 and minor with 12,500. Furthermore, the activity was absorbed by eosinophils but not by macrophages suggesting that eosinophils have receptor sites for ECIF. It was thus suggested that the appearance of ECIF, which is selective for the response of eosinophils to Delayed ECF-a, and decreased Delayed ECF-b production resulted in the inhibition of delayed tissue eosinophilia in FCA-treated guinea-pigs.

Animals↗
PubMed1984

Isolation of an eosinophil chemotactic lymphokine as a natural mediator for eosinophil chemotaxis from concanavalin A-induced skin reaction sites in guinea-pigs.

An intradermal injection with 20 micrograms concanavalin A (Con A) induce a marked tissue eosinophilia peaking at 24 h after the injection in guinea-pigs. Two different eosinophil chemotactic factors (ECFs) were isolated from the Con A-induced skin reaction sites. A non-dialysable ECF with mol. wt of about 70,000 closely resembled delayed ECF-a, which had been isolated from active cutaneous anaphylactic skin lesions and confirmed to be a product of T lymphocytes by antigenic stimulation, by virtue of the antigenicity, the chromatographic profiles on Sephadex G-100 and on DEAE-Sephadex, affinity to Con A-Sepharose, and other physicochemical properties. The activity of the factor paralleled the intensity of tissue eosinophilia, suggesting that the factor may function for the tissue eosinophilia as a natural mediator. Although another ECF with a low mol. wt (dialysable) was isolated from the same skin lesions, the dialysable factor may not contribute to the tissue eosinophilia because the activity of the factor paralleled the intensity of tissue basophilia but not that of eosinophilia.

Animals↗
PubMed1984

[Clinical effect of UFT on urogenital tumors].

Clinical study of UFT which was a mixture of FT and uracil, was conducted on 16 patients with urogenital malignancies. Seven patients had renal cell carcinoma, 5 patients had bladder cancer and 4 patients had prostatic cancer. UFT was continuously administrated at doses of 300 mg or 600 mg per day. One of the patients with renal cell carcinoma and 1 of the patients with bladder cancer showed a complete response, and 1 patient with each cancer showed a partial response, but none of the 4 patients with prostatic cancer responded. In total, complete or partial responses were obtained in 4 of the 16 patients, given an effective rate of 25.0%. Concerning side effects, 3 of the 16 patients complained of anorexia, nausea and vomiting, and stomatitis, but no hepatic or renal disorders, or marrow depression was observed.

Adenocarcinoma↗
PubMed1984

Difference in requirement of macrophage products for concanavalin A-induced production of eosinophil chemotactic factor and macrophage chemotactic factor from guinea pig lymphocytes in vitro.

Differences between the conditions for an eosinophil chemotactic factor (ECF) and macrophage chemotactic factor (MCF) production by lymphoid cells of mesenteric lymph nodes and spleen were studied in guinea pigs. If lymphoid cells were washed less than 4 hr after concanavalin A (Con A) stimulation and were cultured for an additional 24 hr, they failed to produce ECF, whereas Con A stimulation for 1 hr before washing was sufficient to stimulate them to produce MCF. Subsequently, it was shown that heat-labile soluble factors (termed ECF-PF) with potentiating activity for ECF production are produced from macrophages by 5 micrograms/ml Con A activation. When ECF-PF were added to the cell culture with 5 micrograms/ml Con A, the lymphoid cells could produce ECF even when they were washed 2 hr after Con A stimulation and were cultured for an additional 24 hr, suggesting that ECF-PF plays a critical role in the early stage of ECF production. The lymphoid cells were also able to produce ECF even when they were cultured with ECF-PF and a suboptimal dose of Con A (1 microgram/ml) for ECF production. Protein synthesis seemed to be essential for ECF-PF production. The ECF-PF activity was associated with two separated molecular fractions with m.w. of about 50,000 to 70,000 and of 10,000 to 20,000. It is thus suggested that ECF is produced from T cells by Con A stimulation under conditions which differ, at least, from those for MCF in the requirement of ECF-PF.

Animals↗
PubMed1984

[A case of allergic granulomatous prostatitis].

This is a case report of allergic granulomatous prostatitis and its systemic dissemination. A 45-year-old male visited our hospital with initial symptoms of dysuria, pollakiuria and fever on February 19, 1980. Thereafter, a painless abscess in his left cheek and hard swelling of his right parotid gland appeared. He was hospitalized on March 11 because of hearing difficulties and congestion in the conjunctiva. Physical examination revealed no abnormalities in the chest or abdomen, but a proctological examination showed stone-like hardness of the prostate gland which was the size of a hen's egg. Laboratory findings indicated peripheral eosinophilia and immunoglobulinemia. X-rays showed multiple coin lesions in the chest and multiple cystic changes in the spleen. Biopsies of the cheek lesion and prostate showed eosinophilic granulation accompanied by fibrinoid necrosis and vasculitis. When steroid administration was started, his symptoms and signs showed dramatic but temporary improvement. The allergic lesions then gradually progressed and resisted the steroid therapy. He died on March 13, 1982. In 1972, Towfighi et al. presented 31 cases of nonspecific granulomatous prostatitis with a comprehensive review of the literature. They stated that eosinophilic granulomatous prostatitis with both fibrinoid necrosis and vasculitis caused systemic disease with a poor prognosis. Since the pathological findings in our case showed eosinophilic granulation with fibrinoid necrosis and vasculitis, it was a very rare case of the systemic type of eosinophilic granulomatous prostatitis experienced by Towfighi et al.

Dexamethasone↗
PubMed1984

[The diagnosis and pathogenetic study of syringomyelia].

Thirteen cases of syringomyelia which was confirmed by metrizamide CT myelography (MCTM) were reported. Nine cases were associated with Chiari malformation, and four were idiopathic type. Delayed CTM which were performed in several hours after the metrizamide myelography disclosed not only the existence of the syrinx but its shape and location. From the investigation of their clinical findings together with the appearance of their syrinx on CT scan, the following results were obtained. The syrinx was not always situated centrally within the cord, but had laterality at some spinal level in nine out of thirteen cases. The main laterality of the syrinx seemed to be located in the posterior horn. In the cases which had unilateral dissociated sensory disturbance, the laterality of sensory disturbance corresponded with the side of syrinx. These results suggested the possibility that the intrathecal injected contrast materials entered into the syrinx via the dorsolateral sulcus of the cord rather than via IV ventricle in some cases.

Adolescent↗