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Biomedical subjects

K Taniguchi

Publications and source records attributed to K Taniguchi.

At least 883 records · Page 49Linked to original sources
PubMed1983

Analysis of the mechanism of allograft rejection and cell-mediated immunity. II. Divergent effect of CY pretreatment on the generation of cytotoxic activity in the draining lymph nodes and spleen.

CY pretreatment augmented the generation of cytotoxicity in the draining lymph node cells in mice after subcutaneous immunization with allogeneic spleen cells, or in the non-adherent peritoneal exudate cells after intraperitoneal immunization with these cells. Marginal cytotoxicity in the spleen cells after this immunization was suppressed by CY pretreatment. Similar divergent effect of CY pretreatment on the generation of cytotoxic activity in the draining lymph nodes and the spleen was also observed after immunization with allogeneic tumour cells which can induce high degrees of cytotoxicity without CY pretreatment. These results indicate that cytotoxic T lymphocyte (CTL) generation at the site of direct graft rejection appears to have a nature different from that related to CTL generation in the spleen. A discussion is made as to the role of CTL in the peripheral site and the spleen in cases of in vivo allograft rejection.

Animals↗
PubMed1982

Conformational change accompanying transition of ADP-sensitive phosphoenzyme to potassium-sensitive phosphoenzyme of (Na+,K+)-ATPase modified with N-[p-(2-benzimidazolyl)phenyl]maleimide.

The addition of Mg2+ or ATP to (Na+,K+)-ATPase (EC 3.6.1.3) of pig kidney modified with a sulfhydryl fluorescent reagent N-[p-(2-benzimidazolyl)phenyl]maleimide simply reduced fluorescence in the presence of Na+; however, the addition of both ligands to the enzyme induced a reversible dynamic change. The direction of the change was dependent on the concentration of Na+ present. These dynamic changes in fluorescence intensity both in the presence of low and high concentrations of Na+ can be repeated by the re-addition of ATP but not by ADP. Addition of ouabain under the former condition stabilized the fluorescence at the highest level, but the addition of ouabain under the latter condition increased the fluorescence from the lowest to the highest level. The phosphoenzyme formed under the former condition was sensitive to K+ and insensitive to ADP while the phosphoenzyme formed under the latter condition was sensitive to ADP and insensitive to K+. The data indicate that the positive and negative fluorescence changes were induced by the formation of K+-sensitive phosphoenzyme and ADP-sensitive phosphoenzyme, respectively. N-Ethylmaleimide treatment partially inhibited the positive change without affecting the negative change. These data also indicate that the transition of ADP-sensitive phosphoenzyme to K+-sensitive phosphoenzyme accompanied the largest fluorescence intensity change which was examined during the hydrolysis of ATP. The data obtained from the tryptophan fluorescence of both the native and the modified enzyme suggest that the micro-environments of the tryptophan and the sulfhydryl residues are similar in the state of K+-sensitive phosphoenzyme but different in the state of ADP-sensitive phosphoenzyme.

Adenosine Diphosphate↗
PubMed1982

Acute febrile mucocutaneous lymph node syndrome (Kawasaki disease) in an adult.

The patient is a 31-year-old man with a febrile condition accompanied by mucocutaneous changes and swelling of cervical lymph nodes. Clinical features and laboratory data meet the criteria for acute febrile mucocutaneous lymph node syndrome (MCLS). In addition, coronary artery aneurysms and stenotic lesions were observed in the coronary arteriograms. Previously reported cases of MCLS in adults have been extraordinarily rare.

Acute Disease↗
PubMed1982

Mutagenicity of 2,4-dinitrotoluene and its metabolites in Salmonella typhimurium.

2,4-Dinitrotoluene (2,4-DNT) and its metabolites (2A4NT, 4A2NT, 2,4-DNB, 2A4NB, 4A2NB, 2,4-DAT, 2N4AAT, 2A4AAT, 2A4AABA and 2,4-DNBA), and 2,4-dinitrobenzaldehyde (2,4-DNA1), putative metabolite of 2,4-DNT, were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100. 2,4-DAT, 2N4AAT, 2A4AAT, 2A4AABA and 2,4-DNBA were inactive for strains TA98 and TA100. 2,4-DNT itself was only a weak mutagen. Two aminonitrotoluenes (2A4NT and 4A2NT), two aminonitrobenzyl alcohols (2A4NB and 4A2NB) and 2,4-dinitrobenzyl alcohol (2,4-DNB) were increasingly mutagenic in that order, in both strains, however, they were only weak or weak mutagens at mM concentrations. In contrast with these compounds, 2,4-DNA1 was mutagenic even at microM concentrations in both strains. These results suggest that a high mutagenicity of 2,4-DNA1 may be correlated to the carcinogenicity of 2,4-DNT.

Animals↗
PubMed1982

Electrophoretic analysis of RNA segments of human rotaviruses cultivated in cell culture.

Human rotaviruses (HRVs) derived from stools were cultivated to high titres in an established cell line (MA-104) using the rotary culture system. Analysis of the 11 double-stranded RNA segments of the culture-adapted HRVs was carried out by polyacrylamide gel electrophoresis. Tissue culture-adapted HRVs, uncloned or cloned, had the same RNA gel patterns as those of the original HRVs from the individual stool specimens. The migrations of the RNA segments from the culture-adapted HRVs were markedly different from that of the RNA segments from calf rotavirus (Lincoln strain). Considerable heterogeneity in electrophoretic migration of the RNA was found among eight strains of the HRVs grown in cell culture. These results confirmed that viruses isolated from stool specimens were indeed of human origin and were not the result of laboratory contamination with tissue culture-adapted calf rotavirus or other viruses.

Adolescent↗
PubMed1982

Suppressive effect by cooperation of splenic and peritoneal adherent cells on generation of cytotoxic T lymphocytes.

When spleen cells primed in vivo against allogeneic lymphoid cells were used as responder cells in secondary mixed lymphocyte cultures, a high degree of cytotoxicity was generated even in the absence of splenic adherent cells. However, removal of adherent cells from such primed responder spleen cells reduced the cytotoxicity to some extent. On the other hand, when these responder cells were transferred into the peritoneal cavity of irradiated syngeneic mice together with antigenic cells, unseparated responder cells generated a lower degree of cytotoxicity than did adherent cell-depleted responder cells. In an in vitro system, peritoneal adherent cells also suppressed the generation of cytotoxic T lymphocytes by unseparated responders; however they augmented the cytotoxic T lymphocyte generation by adherent cell-depleted responders. These adherent cell populations with augmenting activity became inhibitory when they coexisted. The mechanism of this inhibitory action remains unclear.

Animals↗
PubMed1982

Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains.

Human rotaviruses were isolated directly from stool specimens of gastroenteritis patients in MA-104 cells in the presence of trypsin. For the plaque assay of the isolated strains, the optimal composition of overlay medium was determined. The antigenicity of the isolated strains was investigated by a plaque neutralization method, using antisera prepared against six strains having different electropherotypes of viral RNA, and three different neutralization serotypes were demonstrated.

Antigens, Viral↗