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Biomedical subjects

K Tani

Publications and source records attributed to K Tani.

At least 343 records · Page 19Linked to original sources
PubMed1985

Germinability of coat-lacking spores of Bacillus megaterium.

Upon treatment with acid, the germinability of both intact and coat-lacking spores of Bacillus megaterium ATCC 19213 exhibited similar features. Namely, when the spores previously germinated by alanine in the presence of phosphate buffer were converted to H-spores by treatment with nitric acid, germination proceeded at a very low speed in a same germination medium. When H-spores converted to Ca-spores by treatment with calcium acetate and subsequently germinated, germination proceeded at a speed higher than that of native spores and occurred even in the absence of buffer. These results suggest that the site of exchangeable cations concerned with germinability must not exist in the coat.

Alanine↗
PubMed1985

Molecular cloning and structure of an autosomal processed gene for human phosphoglycerate kinase.

The human genome contains several DNA sequences homologous to cDNA for X-linked phosphoglycerate kinase (PGK). In the course of screening a genomic library containing a mutant gene for X-linked PGK (PGK-Matsue), we obtained a recombinant clone that cross-hybridizes to PGK cDNA. Restriction analysis suggests that the clone overlaps with a clone previously found to originate from chromosome 6, and which has been suggested to code for testis-specific PGK [Szabo et al., Proc. Natl. Acad. Sci. USA 81 (1984) 3167-3169]. The DNA fragment we have cloned contains 1252 bp that are homologous to the coding part of human PGK cDNA, and has an initiation codon, an in-phase termination codon, direct flanking repeats, and no introns. The maximum homology between the DNA and PGK cDNA is 85% at the coding nucleotide level, and it is 86.2% at the translated protein level; homology is also found in their 3'-flanking sequences and adenine-rich tails. These results suggest that the cloned DNA is most likely a processed pseudogene for PGK located on chromosome 6.

Base Sequence↗
PubMed1985

Pathologic features of Behçet's syndrome: a review of Japanese autopsy registry data.

The data recorded from 170 autopsies of patients with Behçet's syndrome in Japan during the period from 1961 to 1976 were analyzed. The patients had been in the second to the eighth decade of life, and the ratio of males to females was 5 to 2. A wide spectrum of pathologic findings was observed, with involvement of neurologic, ophthalmic, cardiovascular, pulmonary, gastrointestinal, visceral, genitourinary, and mucocutaneous systems. Some of the common acknowledged clinical features of the syndrome, such as oral ulcers, synovitis, retinal and cutaneous vasculitis, and venous occlusions, were underreported. This apparent discrepancy in an autopsy series may be due to the effects of treatment or the healing process, as well as to the possible incompleteness of the postmortem examination. The accessibility of recorded data in a national autopsy registry offers a unique opportunity for review of the pathologic features of Behçet's syndrome.

Adult↗
PubMed1985

Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2.

Partial cDNA clones encoding human cytosolic aldehyde dehydrogenase (ALDH1) and mitochondrial aldehyde dehydrogenase (ALDH2) were isolated from a human liver cDNA library constructed in phage lambda gt11. The expression library was screened by using rabbit antibodies against ALDH1 and ALDH2. Positive clones thus obtained were subsequently screened with mixed synthetic oligonucleotides compatible with peptide sequences of ALDH1 and ALDH2. One of the positive clones for ALDH1 contained an insertion of 1.6 kilobase pairs (kbp). The insert encoded 340 amino acid residues and had a 3' noncoding region of 538 bp and a poly(A) segment. The amino acid sequence deduced from the cDNA sequence coincided with the reported amino acid sequence of human ALDH1 [Hempel, J., von Bahr-Lindström, H. & Jörnvall, H. (1984) Eur. J. Biochem. 141, 21-35], except that valine at position 161 in the previous amino acid sequence study was found to be isoleucine in the deduced sequence. Since the amino acid sequence of ALDH2 was unknown, 33 tryptic peptides of human ALDH2 were isolated and sequenced. Based on the amino acid sequence data thus obtained, a mixed oligonucleotide probe was prepared. Two positive clones, lambda ALDH2-21 and lambda ALDH2-36, contained the same insert of 1.2 kbp. Another clone, lambda ALDH2-22, contained an insert of 1.3 kbp. These two inserts contained an overlap region of 0.9 kbp. The combined cDNA contained a sequence that encodes 399 amino acid residues, a chain-termination codon, a 3' untranslated region of 403 bp, and a poly(A) segment. The deduced amino acid sequence was compatible with the amino acid sequences of the tryptic peptides. The degree of homology between human ALDH1 and ALDH2 is 66% for the coding regions of their cDNAs and 69% at the protein level. No significant homology was found in their 3' untranslated regions.

Aldehyde Dehydrogenase↗
PubMed1985

Normal mRNA content in a phosphoglycerate kinase variant with severe enzyme deficiency.

A phosphoglycerate kinase variant, PGK-Matsue, was associated with a severe enzyme deficiency, congenital nonspherocytic hemolytic anemia, and mental disorders. The variant enzyme exhibited a slower cathodal electrophoretic mobility and lower affinity toward the substrates. The enzyme activity in the variant's red cells, muscles, and fibroblasts was about 5% of that of normal cells. The content of mRNA in the variant fibroblasts was compared to that of normal cells by the semiquantitative dot hybridization method, and, more accurately, by the liquid hybridization method, using a human PGK cDNA as a probe. It was found that the mRNA level in the variant fibroblasts was comparable to that of normal fibroblasts. The results strongly suggest that the major cause of enzyme deficiency in PGK-Matsue is a seven- to 10-fold increase in the mutant enzyme degradation.

Cell Line↗
PubMed1985

Pathologically defined neuro-, vasculo-, entero-Behçet's disease.

Behçet's disease is characterized clinically by the presence of a diagnostic triad of stomatitis, genital ulceration and uveitis. Multiple other organ systems may be involved. Prognosis is related to the presence of central nervous system, vascular or intestinal involvement. This report describes the pathology of neuro-, vasculo- and entero-Behçet's disease in a single patient.

Adult↗
PubMed1984

Blunt injury of duodenum with avulsion of papilla of Vater--report of a case.

In a 52-year-old man, there was a complete separation of the duodenum with avulsion of the papilla of Vater from the head of the pancreas due to blunt abdominal trauma. He was successfully treated by an anastomosis of the ampulla to the jejunum of a Roux-en Y limb, after removal of the entire duodenum and partial gastrectomy, followed by gastrojejunostomy of the Billroth II-type, instead of pancreaticoduodenectomy. The patient is well at 15 months after this surgery.

Ampulla of Vater↗
PubMed1984

Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase.

We have determined the sequence of an 812-bp BamHI-EcoRI restriction fragment containing the 5' region of the human gene for PGK (3-phosphoglycerate kinase or ATP:3-phospho-D-glycerate 1-phosphotransferase; EC 2.7.2.3). The fragment contains 450 bp 5' to three start points for transcription (located by primer extension and S1 nuclease mapping), a leader sequence 85-94 bp long, the first exon of gene (65 bp), and part of the first intron. The promoter region is extremely G + C-rich, lacks a TATA box, and has an 8-bp direct repeat. A comparison of the promoter region for PGK with other promoters on the X-chromosome reveals homology with the promoter for HPRT, but not with the operator for factor IX.

Base Sequence↗