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Biomedical subjects

K Tamai

Publications and source records attributed to K Tamai.

At least 217 records · Page 12Linked to original sources
PubMed1988

Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody.

Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr values of brain and adrenal-medulla enzyme were both estimated to be approx. 100,000 by gel-permeation chromatography. SDS/polyacrylamide-gel electrophoresis revealed a single band with an apparent Mr of 50,000. Western immunoblot analysis showed that the antibody recognized each enzyme. With regard to substrate specificity, pH-dependence and effect of pyridoxal 5'-phosphate as a cofactor, both enzymes were similar.

Adrenal Medulla↗
PubMed1988

Three-dimensional geometric analysis of the metacarpophalangeal joint.

No prosthesis yet designed for replacement of the metacarpophalangeal (MCP) joint incorporates all considerations of anatomy and geometry of the natural joint to restore the normal kinematics. Loss of stability, recurrent deformity, and early loosening are persistent problems. In this study, a detailed three-dimensional geometric analysis of the MCP joint was performed. The sagittal contours of both metacarpal heads and base of the proximal phalanx under loaded and unloaded conditions were analyzed. The geometric parameters of the MCP joint articulating surfaces were analyzed, including the center and radius of curvature and the location and size of the contact area. The pressures on articulating surfaces during various hand functions were also established. These findings provided important information for better understanding the kinematics of finger joint articulation, as well as the cause of joint degeneration.

Adult↗
PubMed1988

The effects of vasoactive agents on the proliferation of vascular smooth muscle cells grown in vitro.

The present study was designed to investigate the effect of vasoactive agents on cellular proliferation in serially passed cultured vascular smooth muscle cells (VSMC). A substantial reduction in the number of vascular smooth muscle cells was observed with the addition of nifedipine, nicorandil, bunazocine and labetalol compared with that in a control sample. Furthermore, noradrenaline significantly increased the number of vascular smooth muscle cells. In contrast, neither propranolol nor captopril had any effect on number of vascular smooth muscle cells. The cell size, measured as water volume of vascular smooth muscle cells based on the equilibrium distribution of 3-O-(14C-methyl)-D-glucose, did not differ between treatments with the above-mentioned agents. It is suggested that in addition to a known calcium-mediated mechanism, an alpha-receptor-mediated property could be involved in the proliferation of vascular smooth muscle cells and that clinical use of a calcium antagonist or an alpha-blocker might be useful to prevent the hyperproliferation of vascular smooth muscle cells commonly seen in the vascular walls of patients with hypertension.

Amiloride↗
PubMed1988

New enzymatic determination of sialic acid in serum.

This enzymatic method for determination of sialic acid involves use of neuraminidase (EC 3.2.1.18), N-acetylneuraminate lyase (EC 4.1.3.3), acylglucosamine 2-epimerase (EC 5.1.3.8), N-acetylhexosamine oxidase (from Pseudomonas sp.), and peroxidase (EC 1.11.1.7). Because the method does not require pyruvic acid in the assay medium, interference by pyruvic acid in serum can be avoided. This simple, accurate assay is little affected by other substances in serum.

Carbohydrate Dehydrogenases↗
PubMed1987

Isolation of Mycoplasma pneumoniae from pleural fluid and/or cerebrospinal fluid: report of four cases.

Mycoplasma pneumoniae was isolated from pleural exudates of 2 children with pleuropneumonia. The organism was also isolated from cerebrospinal fluid of a child with meningeal manifestations and from the cerebrospinal fluid as well as pleural exudate of another child who died 21 days after onset of disease. More extensive attempts to cultivate M. pneumoniae from nonpulmonary sites are recommended.

Child↗