Search PubMed⌕ Search

Biomedical subjects

K Takase

Publications and source records attributed to K Takase.

At least 235 records · Page 13Linked to original sources
PubMed1988

Antagonistic effects of alpha-adrenoceptor blocking agents on reticuloruminal hypomotility induced by xylazine in cattle.

The intravenous injection of a standard dose (0.05 mg/kg) of xylazine inhibited reticuloruminal motility in cattle. Pretreatment with adrenoceptor antagonists showing alpha 2-blocking activity, tolazoline (0.5 mg/kg) and yohimbine (0.2 mg/kg), antagonized the xylazine-induced reticuloruminal amotility. Tolazoline was more effective than yohimbine, since an antagonistic effect was not seen at 0.5 mg/kg yohimbine, and yohimbine at 0.2 mg/kg was less effective than tolazoline at 0.5 mg/kg. An adrenoceptor antagonist showing alpha 1-blocking activity, prazosin, did not prevent the inhibition of reticuloruminal motility by xylazine. The xylazine-induced reticuloruminal amotility was also not prevented by either a dopamine receptor antagonist, domperidone, or an opiate receptor antagonist, naloxone. These results suggest that xylazine inhibits bovine reticuloruminal motility through its activation of alpha 2-adrenoceptors, and show that tolazoline can be used as a specific antagonist of xylazine in studies of the alpha-adrenergic influence on reticuloruminal motility in cattle.

Adrenergic alpha-Antagonists↗
PubMed1988

A novel leukemia cell line, MR-87, with positive Philadelphia chromosome and negative breakpoint cluster region rearrangement coexpressing myeloid and early B-cell markers.

We developed a Philadelphia chromosome (Ph) positive cell line, designated MR-87, from a 4-year-old boy with Ph+-acute leukemia. MR-87 cells grew in single cell suspensions with a doubling time of 120 to 144 hours. Both MR-87 and original leukemia cells were positive for myeloperoxidase (MPO) and myeloid antigen CD13. These cells exhibited the early B-cell phenotype, ie, terminal deoxynucleotidyl transferase+, Ia+, CD19+, and CD10+. Rearrangement of the immunoglobulin heavy chain was confirmed in both. Approximately 80% of MR-87 cells coexpressed CD13 and lymphoid antigens CD10 or CD19, as confirmed by a two-color analysis. Simultaneous expression of MPO and CD19 on a single MR-87 cell was demonstrated at ultrastructural level. Thus, MR-87 is a Ph+ leukemia cell line exhibiting a hybrid phenotype. The breakpoint cluster region (bcr) was not rearranged in the MR-87 cells and subsequent analysis using antisera revealed that these cells expressed a novel protein, P190c-abl, which was immunoprecipitated with anti-abl and anti-phosphotyrosine antibodies. The MR-87 line will be most useful for investigating the biology and pathogenesis of Ph+ bcr- acute leukemia.

Antigens, Differentiation, B-Lymphocyte↗
PubMed1988

[Report of a case of intraosseous meningioma].

Cases of intraosseous meningioma appear to be very rare. In the present paper, we report such a case and discuss its etiological histogenesis on the basis of a review of 26 cases previously reported. A 71-year-old female was admitted to our department because of a painless mass in the right parietal region. Neurological findings were normal. Plain skull radiograph showed a 6 X 5-cm osteolytic lesion in the right fronto-parietal bone. CT scan demonstrated this lesion as a mass showing homogeneous enhancement with contrast medium. A right common carotid angiogram showed an avascular area in the parietal region and no tumor stain. The tumor, which was partly attached to the underlying dura, was totally removed surgically, and the postoperative course was uneventful. Histological examination of the specimen revealed fibroblastic meningioma, which was limited only to the outer membrane of the dura. The inner membrane was intact and free of tumor invasion. The histogenesis of intraosseous meningioma is still controversial but the following theories seem to be widely accepted: (1) The tumor arises from a part of the dura which has become trapped in a suture during embryological development. (2) The tumor arises from a part of the dura which has become trapped in a suture due to pressure on the head during delivery. (3) The tumor arises from a part of the dura which has become trapped in the bone due to trauma. (4) The dura which has become trapped in a suture during embryological development may undergo neoplastic change following traumatic stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1987

Cytoplasmic CD3 antigen and T cell receptor gene rearrangement in surface CD3 negative T cell malignancy.

In the present study, it was our intention to further the characterization of the neoplastic cells at the early stage of the T lineage, which were defined as those which bore pan-T marker(s) (CD2, CD5 and CD7) but not CD3 antigen on the surface. We studied six such cases of leukemia and two such cases of lymphoma for their phenotypes including cytoplasmic CD3 detected with flow cytometry and for the rearrangement of T cell receptor and immunoglobulin genes. The cytoplasmic expression of CD3 antigen in adult thymic cells was also studied. CD7 was expressed in seven cases, the exception being one presumably of B lineage, and rearrangements of T cell receptor gene were detected in five cases. Four cases out of these five genotypic T neoplasms had surface phenotypes compatible with the stage of thymic cells and, interestingly, they all displayed CD3 in the cytoplasm. With regard to normal cells, cytoplasmic CD3 was shown to be present only in a small population of surface CD3 negative thymic cells. These malignant cells, therefore, may have originated from such cells. The exact origins of the two cases bearing pan-T marker(s) with no rearrangement of the T cell receptor gene has not yet been determined.

Adolescent↗
PubMed1986

Antagonism of the emetic action of xylazine by alpha-adrenoceptor blocking agents.

The intramuscular injection of xylazine (2 mg/kg) evoked vomiting in 81% of the dogs studied. Adrenoceptor antagonists showing alpha 2-blocking activity, yohimbine, tolazoline and phentolamine, antagonized the xylazine-induced vomiting in a dose-dependent manner. Of these antagonists, yohimbine was the most effective, since the maximal antagonistic effect was seen at 0.5 mg/kg yohimbine, a dose at which the other drugs had less or no effect. The adrenoceptor antagonists showing alpha 1-blocking activity, prazosin and phenoxybenzamine, at the doses studied did not prevent the emesis induced by xylazine. A beta-adrenoceptor antagonists, propranolol, was ineffective in reducing xylazine-induced vomiting. The dopamine receptor antagonists, metoclopramide and domperidone, did not prevent xylazine-induced vomiting nor did yohimbine antagonize apomorphine-induced vomiting. The xylazine-induced vomiting was not prevented by atropine, naloxone or hexamethonium. These results indicate that the xylazine-induced vomiting is mediated by alpha 2-adrenoceptors and does not appear to involve beta-adrenoceptors, cholinoceptors, dopamine or opiate receptors in the emetic pathway.

Adrenergic alpha-Antagonists↗