Search PubMed⌕ Search

Biomedical subjects

K Takano

Publications and source records attributed to K Takano.

At least 271 records · Page 15Linked to original sources
PubMed1993

Characterization of insulin-like growth factor II (IGF-II) and IGF binding proteins in patients with non-islet-cell tumor hypoglycemia.

Insulin-like growth factor II (IGF-II) in serum and tumor extracts from five patients with non-islet-cell tumor hypoglycemia (NICTH) has been characterized. These tumors contained large quantities of IGF-II (2.4-14.2 micrograms/g tissues). The serum IGF-II levels in four of five patients were a little high and the serum IGF-I levels in five patients were low. The serum IGF-II/IGF-I ratios in these patients ranged from 24.1 to 64.2, and the values were significantly greater than those in normal subjects (1.7-7.1). When the sera were gel-filtered on a Sephacryl S-200 column under neutral conditions, the proportion of the free form of IGF-II was not increased. However, in four of five patients, an abnormal IGF-II-IGF binding protein complex was found. When serum IGF binding proteins (IGFBPs) were analyzed by Western ligand blotting, serum IGFBP-2 increased in these patients. When the tumor extracts and sera were gel-filtered on a Biogel P-60 column under acidic conditions, the majority of IGF-II in these sera was a big form of IGF-II. As compared to authentic IGF-II, insulin receptor reactivities and IGF-II receptor reactivities of tumor extracted IGF-II increased in two of three patients. These data indicate that in patients with NICTH, heterogenous IGF-II is produced in respect of size and bioactivities, and that the characteristics of IGF binding protein are altered. Thus, to find IGF-II producing tumors among extrapancreatic tumors associated with hypoglycemia, the quality of IGF-II as well as the quantity should be studied.

Aged↗
PubMed1993

Responses of insulin-like growth factor binding protein-1 (IGFBP-1) and the IGFBP-3 complex to administration of insulin-like growth factor-I.

The importance of insulin-like growth factor binding proteins (IGFBPs) in modulating the bioactivity of administered IGFs is poorly understood. This study examines responses of IGFBP-1 and the IGFBP-3 complex to recombinant human IGF-I. Eight fasted subjects received a single dose of 0.1-0.125 mg/kg IGF-I sc. This caused a 10-fold rise in IGFBP-1 over 6 h, falling rapidly after food intake. Peak (6-h) IGFBP-1 values were highly correlated with peak post-prandial (8-h) glucose values (r = 0.941). IGFBP-3 showed little response, decreasing slightly over the 48-h period following IGF-I. Adaptive changes in IGFBPs were studied in fed adults injected daily for 7 days with IGF-I, 0.1 mg/kg sc. Following the first injection, IGFBP-1 had a markedly blunted response compared to that in fasted subjects. However, after the seventh IGF-I injection, a 3.5-fold greater IGFBP-1 response to the same IGF-I dose was seen. Concomitantly with the increased IGFBP-1 responsiveness, mean immunoreactive IGFBP-3 and acid-labile subunit levels decreased significantly (p < 0.005), whereas IGFBP-2 detected by immunoblotting increased. Thus IGF-I administration causes changes in IGFBPs which may be important in regulating IGF-I bioavailability.

Adult↗
PubMed1993

Hereditary hydronephrosis in C57L/MsNrs mice.

Inbred C57L/MsNrs mice (66 males and 72 females) maintained at the National Institute of Health, Japan, were examined grossly for hydronephrosis. Unlike the hydronephrotic strains of mice reported so far, C57L mice showed no sex-related difference in the incidence (62% in males and 75% in females; P > 0.05) and severity of hydronephrosis. The right kidney was more severely affected than the left kidney in both sexes. Age appeared to have no influence on the incidence.

Animals↗
PubMed1993

[Experiment on validity of skin resistance level as an index of arousal level].

It is important in establishing and improving work management and work planning to estimate the arousal level of workers during operation and monitoring tasks. This paper proposes a technique based on experimental results to estimate the arousal level during job execution. In this experiment, both a monotonous low workload task which degrades the arousal level and a high workload task which has an awakening action were alternately applied to five subjects for three hours. Skin resistance level (SRL) as an index of arousal level of the autonous nervous system was measured with subjective drowsiness and task performance, that is, signal omission probability for low workload task and both reaction time and error probability for high workload task, as indexes of arousal level of the central nervous system. The following results were obtained. (1) The results suggested that SRL as an index of arousal level of the autonous nervous system is significantly related and correlated with subjective drowsiness and signal omission probability as indexes of arousal level of the central nervous system. (2) The average reaction time and the error probability tended to increase by the application of high workload when the arousal level was degraded. The influence of degraded arousal continued for a short time and showed an unfavorable effect on task execution. The degree of this influence, however, differed between subjects. (3) SRL can be used as an index of arousal level of the autonous nervous system because the measurement is comparatively easy and is little influenced by artifacts due to body movements. (4) Using SRL at initial high workload period as the standard value, it is possible to quantify the SRL value at arbitrary time by estimating how many times the standard value goes into the SRL value. Based on this quantified value as an index of arousal level, comparison with task performance can be made. The proposed index of arousal level of the autonous nervous system showed a significant correlation with indexes of the central nervous system. This technique can be applied to various kinds of estimation on human characteristics accompanied with changes in arousal level.

Adult↗
PubMed1993

[A study of clinical pictures and monoamine metabolism of Gilles de la Tourette syndrome].

Gilles de la Tourette Syndrome (Tourette syndrome) is a movement disorder characterised by both motor and vocal (phonic) tics, accompanied by coprolalia and frequently echolalia. Since Seignot discovered that administration of haloperidol (HPL) was effective in treating Tourette syndrome, the etiology of this syndrome has been hypothesized as abnormal metabolism in neurotransmitters. We studied the clinical pictures on nine typical cases of Tourette syndrome and measured the HVA, MHPG, and 5-HIAA in their cerebrospinal fluid (CSF). Diagnosis of Tourette syndrome were made of the nine cases (all males) by DSM-III-R. All cases had symptoms of either coprolalia or echolalia. Four cases had no history of treatment with neuroleptics (untreated group) and five cases were under treatment with HPL (treated group). CSF was obtained from each subject in the morning (between 9:00-9:30 a.m.), at rest with nothing by mouth since last night and it was rapidly frozen and stored at -80 degrees C. HVA, the metabolite of dopamine, MHPG, the metabolite of norepinephrine, and 5-HIAA, the metabolite of serotonin, were analyzed by HPLC. The same analyses were made in five males who were healthy volunteer controls (control group). Comparisons were made of the HVA, MHPG and 5-HIAA levels between the three groups (untreated, treated and control group). HVA was 63.5 +/- 8.4 ng/ml in the untreated group, which was significantly higher than the 35.6 +/- 8.6 ng/ml in the controls (p < 0.05). No significant difference was found in both MHPG and 5-HIAA between the untreated and control group. No significant differences were found in HVA or MHPG between the treated group and the controls. The treated group had significantly lower 5-HIAA levels than the controls (p < 0.05). A significantly lower levels of CSF HVA were found in the treated group than in the untreated group (p < 0.05), but there were no significant differences in MHPG or 5-HIAA in the treated and untreated group. Assuming that the untreated group was most indicative of Tourette syndrome during the study, higher levels of HVA may indicate dopaminergic hyperfunction.

Adolescent↗
PubMed1993

[Recent progress in diagnosis and treatment of patients with GH deficiency and GH overproduction].

The diagnostic criteria of GH deficiency and acromegaly have been revised in 1990 by the Ministry of Health and Welfare in Japan. According to this criteria, not only the classical type of severe GH deficiency but also partial GH deficiency can be diagnosed and treated. With development of technology, anatomical abnormalities could be seen by MRI and genetic abnormality analysed. For the treatment of patients with GH deficiency, the pen-system has becomes available, which makes the daily injection of GH painless and easy. For the treatment of GH producing pituitary tumor (acromegaly), a long acting analog of somatostatin is now available to reduce the size of the tumor.

Acromegaly↗
PubMed1993

Clinical staging and gadolinium-DTPA enhanced images of the wrist in rheumatoid arthritis.

The usefulness of gadolinium (Gd)-DTPA enhanced magnetic resonance imaging (MRI) of the wrist joint for predicting articular destruction was investigated in 49 patients with rheumatoid arthritis (RA). On T1 weighted images with Gd-DTPA enhancement, the synovium showed a localized or diffuse pattern of enhancement. Many patients classed as stage II or higher by plain radiography had diffuse enhancement on MRI, while all except one stage I patient had relatively confined localized enhancement. Our study showed that MRI of the carpus could detect soft tissue changes in patients with RA earlier than anything revealed by radiography, and suggested that contrast MRI may be useful for predicting articular destruction.

Arthritis, Rheumatoid↗
PubMed1993

[Study of synovial lesions by MRI using gadolinium-DTPA (Gd-DTPA) in patient with early phase of rheumatoid arthritis].

To evaluate the usefulness of magnetic resonance imaging (MRI) enhanced with gadolinium-DTPA (Gd-DTPA) for the detection of the inflamed synovium and for the evaluation of the response to therapy in rheumatoid arthritis, we studied 49 patients with rheumatoid arthritis (RA) according to the 1987 revised criteria of American Rheumatism Association (ARA), 6 patients of systemic lupus erythematosus (SLE) complicated by arthritis, 3 patients of osteoarthritis (OA), 2 patients of Sjögren syndrome, 2 patients of progressive systemic sclerosis and 10 healthy volunteers as an age matched control. The 49 patients with RA were divided into three groups: (1) early phase of RA, (2) non progressing RA and (3) slowly progressing RA, and the stage classification of plain X-ray film and enhancement pattern of MR images were classified into three groups. Synovial enhancement showed a linear, band-like or diffuse pattern. Almost all cases in early phase of RA group and non progressing RA group showed a linear pattern, a band-like pattern or even no enhancement, while slowly progressing group of stage II or higher showed the diffuse pattern of enhancement in all except 2 cases. Moreover, the linear pattern, the band-like pattern or even no contrast enhancement were seen in all except 1 stage I patient, whereas 26 out of 29 patients with stage II or higher change showed diffuse contrast enhancement. Furthermore, a comparison of MR images before and after administration of DMARDs in 10 patients showed that the improvement of clinical symptoms correlated fairly well with reduction of contrast enhancement.(ABSTRACT TRUNCATED AT 250 WORDS)

Arthritis, Rheumatoid↗
PubMed1992

[A case report of serous cystadenoma located in pancreatic tail].

Cystadenomas of the pancreas are relatively rare compared to pseudocysts. There are two types of cystadenoma of the pancreas; mucinous and serous. Serous cystadenomas of the pancreas are very rare. We experienced a serous cystadenoma located in pancreatic tail. The patient was male, forty-one years old, and was free from any subjective symptom. The pancreatic cyst was found by abdominal ultrasonography and was difficult to differentiate serous or mucinous by computed tomography or celiac angiography preoperatively. We performed distal pancreatectomy and the cyst was revealed to be serous cystadenoma pathologically.

Adult↗
PubMed1992

Effects of activin A and somatostatin on intact FSH secretion and intracellular Ca2+ concentration in human FSH-secreting pituitary adenoma cells.

Activin A stimulated synthesis and secretion of intact FSH in dispersed human FSH-secreting adenoma cells. Significant stimulation was observed after 24 hr. Activin A caused an increase in Ca2+ concentration ([Ca2+]i). This response occurred soon after the activin A action. These effects were blocked in Ca(2+)-deficient medium and by nitrendipine (5 microM). Somatostatin inhibited the activin A-induced intact FSH secretion and the [Ca2+]i response. These findings indicated that Ca2+ influx through voltage-gated Ca2+ channel was involved in the activin A induced synthesis and secretion of intact FSH.

Activins↗
PubMed1992

The majority of the marker chromosomes in Japanese patients with stigmata of Turner syndrome are derived from Y chromosomes.

DNA analyses of 41 individuals with stigmata of Turner syndrome and a 45,X/46,X+mar or 46,X+mar karyotype were carried out. Southern-blot analysis employing 17 Y-specific probes were used to determine whether the marker chromosome was Y-chromosomal in origin. Of the 41 DNA samples from these patients, 23 contained detectable Y-chromosomal DNA. Points of chromosome breakage were distributed over the entire length of the Y long arm. Three individuals, who carry different portions of the Y chromosome, had developed gonadoblastoma. GBY (the gonadoblastoma locus on the Y chromosome) is mapped proximal to DYS132, midway between the 13 Yq loci that we have studied. We also used a polymerase chain reaction technique that could detect 7 loci over the length of the Y chromosome. This technique may be useful for the rapid assessment of marker chromosomes, especially for evaluating the risk of gonadoblastoma.

Blotting, Southern↗
PubMed1992

Growth hormone reverses impaired wound healing in protein-malnourished rats treated with corticosteroids.

Corticosteroid (CS) administration amplifies the inhibitory effect of protein malnutrition (PM) on wound healing. Abdominal surgery in protein malnourished patients receiving corticosteroids (eg, advanced malignancy, transplant recipients) may be complicated by wound dehiscence or anastomotic breakdown. Although preoperative parenteral nutrition can reduce the incidence of these complications, this is not possible in patients requiring urgent surgical intervention. In a previous report we demonstrated that postoperative growth hormone (GH) administration could restore normal wound healing in PM rats. This study evaluates the effect of GH on wound healing in PM rats treated with CS. Forty-eight female Sprague-Dawley rats weighing 180 to 210 g were divided into four groups: (1) normally nourished; (2) PM only; (3) PM + CS; and (4) PM + CS + GH. PM rats (groups 2 to 4) received 5.5% protein chow every other day for 8 weeks. Control rats (group 1) received 23.4% protein chow for the same duration. Group 3 and 4 rats received prednisolone (2 mg/kg, intramuscularly) during the last 3 weeks of PM. All animals underwent precise 5-cm midline celiotomies. Postoperatively, rats in all groups were given 23.4% protein chow. Group 3 and 4 rats continued to receive CS postoperatively. Group 4 rats were given GH (0.5 mg/d, intraperitoneally) postoperatively for 5 days. Wound testing was performed on the 6th postoperative day after removal of the sutures. A balloon inserted into the peritoneal cavity through the vagina was gradually inflated. The pressure at which the wound disrupted was recorded as the wound bursting strength.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Cortex Hormones↗