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Biomedical subjects

K Sugano

Publications and source records attributed to K Sugano.

At least 217 records · Page 12Linked to original sources
PubMed1989

[Enhanced effect of hepatic arterial infused anticancer drugs on rabbit VX-2 carcinoma by means of combination with Lipiodol. Adriamycin versus Lipiodol adriamycin sandwich method and adriamycin suspended in a Lipiodol].

To increase drug delivery to a hepatic malignant tumor, various tissue contents and serum levels of adriamycin (ADR) were evaluated in comparison with different intraarterial injection methods of oily or aqueous solution. VX-2 tumor was implanted in the rabbit liver. ADR was infused through the hepatic artery by the following three methods: Group 1: ADR with physiological saline; Group 2: ADR administered between lipiodol injection; and Group 3: urographin-dissolved ADR suspended in lipiodol. ADR concentrations of the tumor, liver, heart, kidney or bone marrow were compared between these three groups one hour after ADR administration. Tumor uptake and ADR concentration ratio of tumor to liver were higher in Group 3 than in the other two groups. ADR concentration ratio of tumor to liver was higher in group 2 than in group 1. Cardiac and renal uptake in group 3 was less than in group 1 and 2, and so infusion of ADR suspended in a lipiodol may serve to reduce side effects of ADR, especially cardiotoxicity.

Animals↗
PubMed1989

[Clinical evaluation of serum level of NCC-ST-439 as a new tumor marker for colorectal diseases--fluctuation of serum NCC-ST-439 in patients with colorectal cancers before and after surgery].

Serum level of NCC-ST-439, a new tumor marker, was clinically evaluated in patients with benign and malignant colorectal diseases in comparison with serum CEA and CA 19-9. Fluctuation of serum levels of NCC-ST-439 in patients with colorectal cancers was especially studied. Serum level of NCC-ST-439 was determined by sandwich type NCC-ST-439 EIA kit, which was developed by Nippon Kayaku Co., Ltd., in 93 cases of primary colorectal cancers, 98 cases of recurrent colorectal cancers and 60 cases of benign colorectal diseases. Positive rate of serum NCC-ST-439 in patients with primary colorectal cancers was 8.6% in Dukes A cases, 14.3% in Dukes B cases, 37.5% in Dukes C cases and 78.9% in Dukes D cases. Positive rate in patients with recurrent colorectal cancers was 42.8%. On the other hand, false positive rate in patients with benign colorectal diseases was 5.0%, which was relatively low in comparison with serum CEA and CA 19-9. While serum NCC-ST-439 was well correlated with serum CEA and CA 19-9 in primary colorectal cancers, NCC-ST-439 value in benign colorectal diseases showed no correlation with serum CEA and CA 19-9. In patients with primary colorectal cancers, curative resection was applied for in 40 cases in which the value recovered to the normal range after resection. In contrast, all 8 cases, which received non-curative resection, showed the increase of serum NCC-ST-439 value. In cases of recurrent colorectal cancers, some cases, which responded against chemotherapy, obviously showed a decrease of NCC-ST-439 value. These facts indicated that serum level of NCC-ST-439 could monitor the clinical effects of surgical treatment, chemotherapy and radiation therapies and it could be used as a marker for colorectal cancers. In conclusion, determination of serum NCC-ST-439 value was clinically useful for the diagnosis and monitoring for patients with colorectal cancers as a new distinct tumor marker.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1988

Region-specific distribution of glycosphingolipids in the rabbit gastrointestinal tract: preferential enrichment of sulfoglycolipids in the mucosal regions exposed to acid.

Neutral and acidic glycosphingolipids from various regions of the rabbit gastrointestinal tract showed different but characteristic patterns. In particular, sulfatides were found to be present in high concentrations in the mucosal regions frequently exposed to gastric acid, such as the gastric mucosa and proximal intestinal mucosa (duodenum and jejunum). The majority of sulfatides in these tissues have been identified as GalCer-I3-sulfate, with alpha-hydroxy long-chain fatty acids, by chemical procedures including analysis by negative ion fast atom bombardment mass spectrometry. The concentrations of GalCer-I3-sulfate in the fundic mucosa, antral mucosa, duodenum, and jejunum were 394.0, 356.9, 829.3, and 378.1 nmol/g of dry weight, respectively. These concentrations exceed those in the kidney, which has been reported to contain the highest concentration of sulfatides, with the exception of the nervous system, also, especially in the fundic mucosa, gangliosides were drastically reduced, in contrast to sulfatides: the molar ratio of sulfatides to GM3, the major ganglioside, was about 11:1. In addition, in the acid hydrolysis experiment, using the same concentration of HCl as that present in the gastric juices, sulfatide proved to be much more resistant to acid than GM3. Therefore, the preferential distribution of sulfatides in the mucosal regions exposed to acid may be related to mucosal functions, such as acid secretion and protection from acidic environments.

Animals↗
PubMed1988

Sandwich radioimmunometric assay with murine monoclonal antibody, NCC-ST-439, for serological diagnosis of human cancers.

Sandwich radioimmunometric assay (RIA) for a new tumor-associated carbohydrate antigen defined by a monoclonal antibody (MoAb), NCC-ST-439, was developed and the antigen levels were determined in sera of normal donors, and patients with various malignant and non-malignant disorders. In normal donors, 97.0% (226/233) of sera were antigen-negative (less than 12 units/ml) except for 7 serum samples from young females. In patients with malignant disorders, 34.2% (82/240) were antigen-positive, in particular 64.0% (16/25) of patients with pancreatic carcinoma, 66.7% (16/24) of patients with recurrent colorectal carcinoma and 54.5% (6/11) of patients with recurrent breast carcinoma. In patients with non-malignant disorders, 6.0% (7/116) were antigen-positive. The positive rate in benign hepatobiliary disorders, including gallstones, hepatitis and liver cirrhosis, was especially low at 4.3% (1/23). We concluded that determination of serum NCC-ST-439 antigen would be useful in serodiagnosis of carcinoma patients.

Adult↗
PubMed1988

Development of gastrin synthesis and posttranslational processing mechanisms in rats.

To examine the ontogeny of gastrin synthesis and posttranslational processing mechanisms, we utilized region-specific antisera to determine the factors that regulate the contents of gastrin and its precursors in the stomachs of developing rats. Gastrin content increased gradually from birth to a peak at 5 wk of age, after which there was a slight decrease to levels observed in mature animals. Unlike gastrin, glycine-extended precursors of gastrin (G-Gly) increased abruptly in the first day of life but thereafter increased at a steady state. Dexamethasone treatment and early weaning (at 11 days of age) rapidly increased gastrin content, whereas G-Gly content was decreased. On weaning animals to adult rat chow abruptly at an age (18 days) when the corticosteroid surge associated with the weaning process had already occurred, evidence for increases in both gastrin and G-Gly content were observed 7 days later. In contrast, administration of dexamethasone to 18-day-old rats enhanced gastrin content to a greater extent than G-Gly. These results suggest that the natural surge in corticosteroids associated with weaning may enhance gastrin amidation activity, whereas the concomitant dietary changes may exert a stimulatory effect of gastrin synthesis.

Aging↗
PubMed1987

Progastrin and its glycine-extended posttranslational processing intermediates in human gastrointestinal tissues.

We examined the posttranslational modification of gastrin in human gastrointestinal and Zollinger-Ellison tumor tissues using antibodies specific for progastrin and its glycine-extended processing intermediates. Gel filtration of antral and duodenal extracts on Sephadex G-50 revealed multiple molecular forms of immunoreactive glycine-extended processing intermediates corresponding to known molecular forms of gastrin and cholecystokinin. Immunoaffinity chromatography studies of antral mucosal and gastrinoma extracts identified a molecular form of glycine-extended processing intermediates that was characterized by an amino terminus identical to that of gastrin heptadecapeptide. Large differences in the relative contents of precursors and products of gastrin synthesis were found in gastrinoma tissue as compared to gastrointestinal mucosal extracts. These studies suggest the potential importance of glycine-extended peptides as intermediates in the posttranslational processing of gastrin and cholecystokinin in humans and indicate that gastrin processing mechanisms may be altered in Zollinger-Ellison tumors.

Chromatography, Affinity↗