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Biomedical subjects

K Sugano

Publications and source records attributed to K Sugano.

At least 199 records · Page 11Linked to original sources
PubMed1990

Bronchogenic lung cyst with elevated serum type I chain related carbohydrate antigens, CA19-9, CA50 and FH-7.

A case of bronchogenic cyst of the right lung in a 30-year-old woman who had elevated serum type 1 chain related carbohydrate antigens defined by the three monoclonal antibodies, CA19-9, CA50 and FH-7, is reported. The mucinous fluid from the cystic lesion contained extraordinary high antigen levels, and immunohistochemistry revealed CA19-9 to be highly positive in the cystic wall and cyst fluid. Antigen levels returned to their normal ranges after a right lower lobectomy. This represents a rare case of bronchogenic cyst with abnormal increases in the respective carbohydrate antigens.

Adult↗
PubMed1990

Characterization of carcinoembryonic antigen-related antigens in normal adult feces.

About 50-70 mg in total of carcinoembryonic antigen (CEA) (or CEA-related antigens) was detected in normal adult feces evacuated during one day (200-250 g). Ten percent or less of the antigen was found to be in soluble form in fresh feces (naturally solubilized antigen), while 90% or more was still in membrane-bound form which was releasable with phosphatidylinositol-specific phospholipase C (PI-PLC-solubilized antigen). The naturally solubilized and PI-PLC-solubilized antigens are antigenically different from each other and similar to normal fecal antigen (NFA)-2 and CEA, respectively, suggesting that "CEA-distinctive" antigenicity detected so far in CEA from cancerous tissues is not due to the difference between antigens in normal and malignant tissues but is probably due to the presence of the glycosylinositolphosphate moiety at the carboxyl-terminus of the antigen molecule. Thus, "CEA-distinctive" antigenicity is by no means cancer-specific, but this antigenicity seems to be critical for the clinical significance of CEA as a tumor marker, because an assay system (Kit II) which is able to distinguish CEA from NFA-2 revealed much improved features in cancer diagnosis as reported recently.

Adult↗
PubMed1990

[Regulation of secretion and synthesis of gastrointestinal hormones: studies with cultured gut endocrine cells].

Small number of endocrine cells are diffusely distributed in the gut mucosa. Studies on their secretory mechanisms have been further complicated by numerous neural, paracrine, and endocrine factors affecting their response. Recent technical development for isolation and culture of gut endocrine cells has circumvented these problems and enabled to study their receptors, signal transduction mechanism, and biosynthesis of gut hormones. In this review, current progress made in the cellular physiology of gut endocrine cells is summarized.

Animals↗
PubMed1990

[Two cases of advanced gastric cancer with diffuse carcinomatosis of the bone marrow showing a widespread osteoplastic bone metastasis].

Reported are 2 cases of an advanced gastric cancer with a "diffuse carcinomatosis of the bone marrow" (DCMB) showing a widespread osteoplastic bone metastasis. In a DCBM, a widespread invasion of cancer cells to the bone marrow occurs, causing leukoerythroblastosis, anemia, and disseminated intravascular coagulation. The local reaction in the bone, namely being osteoplastic or osteolytic, has not been discussed in previous papers. Our cases were characterized by an extremely high serum alkaline phosphatase, a "superscan" view of the bone scintigram, and a diffuse osteoplastic bone metastasis with an osteoid increase. Thus we think there are DCBM subtypes with a diffuse osteoplastic bone metastasis.

Adenocarcinoma, Mucinous↗
PubMed1990

Glycosphingolipid composition of the gastric mucosa. A role of sulfatides in gastrointestinal mucosal defense?

The glycosphingolipid (GSL) compositions of the human, rabbit, and rat gastric mucosa were studied and compared. In neutral GSLs, although GalCer was the most abundant component in every tissue examined, species-specific differences were clearly observed in their composition. Acidic GSLs were composed of sulfatides and gangliosides. Every gastric mucosa contained sulfatides (GalCer-sulfate) in a high concentration. The concentrations of sulfatides per gram of dry weight were 416.0, 933.8, 394.0, 365.9, and 135.9 nmol for the human fundus, human antrum, rabbit fundus, rabbit antrum, and rat glandular stomach, respectively. On the other hand, gangliosides were less in these tissues. The molar ratios of sulfatides to GM3, a major ganglioside, were 4.3, 6.7, 11.1, 2.1, and 1.2 for each gastric mucosa described above, respectively. A high sulfatide concentration in animal gastric mucosa, with its structural similarity to sucralfate, implies that sulfatides may play an important role for gastrointestinal mucosal protection.

Animals↗
PubMed1989

Glycine-extended processing intermediates of gastrin and cholecystokinin in human plasma.

The biosynthesis of biologically active gastrin and cholecystokinin (CCK) requires the formation of carboxyl-terminally amidated peptides from glycine-extended precursors of gastrin and CCK. In previous studies we and others have identified and characterized glycine-extended forms of gastrin (Ggly) and CCK (CCK-gly) in the human gastrointestinal tract. To explore the potential biologic importance of these peptides in humans, we examined their release into the circulation. Ingestion of a standard meal induced a biphasic rise in plasma G/CCK-gly concentration, but only the initial increase correlated with gastrin release. Intraduodenal lipid infusion caused a selective rise in CCK-gly immunoreactivity with no increase in gastrin or G-gly. Gel filtration chromatography revealed that the predominant molecular form of G/CCK-gly in basal plasma coeluted with CCK8-gly, but in response to meal stimulation, increases in other molecular forms were noted. Measurement of glycine-extended intermediates of progastrin and procholecystokinin posttranslational processing in plasma may aid in determining their physiologic importance in health and disease.

Adult↗
PubMed1989

Detection of increased fecal carcinoembryonic antigen and its characterization as a membrane-bound form in colorectal carcinoma and other gastrointestinal disorders.

The levels and character of carcinoembryonic antigen (CEA) in feces were investigated by sandwich radioimmunometric assay using anti-CEA monoclonal antibodies NCC-CO-411 and NCC-CO-432. Mean CEA concentration was significantly higher (P less than 0.001) in the feces from patients with colorectal carcinoma and other gastrointestinal disorders as compared to normal adults. More than 90% of the fecal CEA was trapped by a 0.22 micron membrane filter and solubilized by treatment with 1% Triton X-100 or phosphatidyl-inositol specific phospholipase C. In hydrophobic chromatography, most of the fecal CEA was eluted at the lowest (NH4)2SO4 concentration while serum CEA appeared in the more hydrophilic fractions. These results suggest that the majority of CEA exists in feces as an amphiphilic molecule or a membrane-bound form. The increase of fecal CEA may reflect the destruction and abrasion of epithelial cells in various gastrointestinal disorders.

Antibodies, Monoclonal↗
PubMed1989

Intracellular topography of glycine-extended pro-gastrin-processing intermediates in human antral mucosa: an electron-microscopic immunocytochemical study.

To identify and characterize the subcellular topography of glycine-extended pro-gastrin-processing intermediates (G-Gly) in human antral mucosa, we performed an electron microscopic immunocytochemical study using region-specific antisera generated against the synthetic peptide, Tyr-Gly-Trp-Met-Asp-Phe-Gly (GL7), and C-terminal-specific anti-gastrin antisera. As has been previously reported, G-cells contained both electron-dense and electron-lucent granules, with a range of intermediate forms. Gastrin immunoreactivity was demonstrated in almost all granules of each type, whereas anti-GL7 antisera immunostained chiefly electron-dense granules. The relative ratio of GL7/gastrin granules varied among different cells but was approximately 1:10 on average. Other cytoplasmic organelles were devoid of specific labeling for GL7 or gastrin. As we have assumed that G-Gly serves as the immediate precursor for each molecular form of gastrin, electron-dense granules with high labeling for GL7 are regarded as the principal site for conversion of G-Gly to gastrin. This speculation supports many previous reports that electron-dense granules are immature and that the granules become less electron-dense with maturation.

Adult↗
PubMed1989

[Dental health measures to psychopathic patients. II. The improvement of toothbrushing habit and its related factors].

This study was designed for an oral prophylaxis program to remedy the oral condition of the mentally subnormal patient who required treatment at an open type mental hospital. The subjects consisted of 115 inpatients. The mean age was 45.5 years. The mean number of teeth functioning in mastication was 19.6. In order to improve their oral health they were given guidance and their oral hygiene condition was inspected every month. "STPI" was applied to investigate the toothbrush behavior in these patients. After six months, oral condition improved. Relationship between toothbrush behavior and mental illness was analyzed by principle component analysis and rotated by normal valimax method. Four factors were designed as follows. (1) Depressional state; (2) Personality disorders; (3) Hypochondric factor; (4) Manic state. Dental health of chronically mentally ill patients related to suppressive toothbrush behavior. The other hand the findings of this study showed that it was possible to improve their oral health by improving their living condition.

Adolescent↗