Search PubMed⌕ Search

Biomedical subjects

K Shiomi

Publications and source records attributed to K Shiomi.

At least 163 records · Page 9Linked to original sources
PubMed1985

Detection of tetrodotoxin-like compounds in two species of puffer fishes (Lagocephalus lunaris lunaris and Fugu niphobles).

The toxins in the muscle, skin and liver of the puffer fish Lagocephalus lunaris lunaris and those in the skin and liver of another puffer fish Fugu niphobles were purified by successive column chromatography on activated charcoal, Bio-Gel P-2 and Bio-Rex 70 and analyzed by electrophoresis and thin layer chromatography. Although, regardless of the tissues and species, tetrodotoxin was predominant and accounted for more than 90% of the total toxins, the following minor toxins were newly detected; toxin A and toxin B in L. lunaris lunaris and toxin C in F. niphobles. Since these minor toxins, like tetrodotoxin, were positive to the Weber reagent and 10% KOH, but negative to 1% H2O2, they were assumed to be tetrodotoxin-like compounds. Interestingly, the electrophoretic and thin layer chromatographic analyses showed that toxin A and toxin C are probably identical with AFT3 and AFT1, the minor toxins in the crab Atergatis floridus from Chiba, Japan, respectively.

Animals↗
PubMed1982

Volatile sulfur compounds responsible for an offensive odor of the flat-head, Calliurichthys doryssus.

1. The volatiles of a flat-head, Calliurichthys doryssus, which gives out a characteristic offensive odor in living time, were analyzed by gas chromatography. 2. A large quantity of volatile sulfur compounds was detected in the flat-head; of which methyl mercaptan and/or dimethyl disulfide were judged to be responsible for the offensive odor. 3. The contents of methyl mercaptan and dimethyl disulfide were much higher in skin than in muscle and viscera.

Animals↗
PubMed1979

Purification and characterization of an agglutinin in the red alga Agardhiella tenera.

The red alga, Agardhiella tenera was found to contain a glycoprotein which agglutinates mouse leukemia cells, L5178Y but not L1210. It also agglutinates guinea pig and rabbit erythrocytes, and has weak activity against human A, B and O, mouse, horse and sheep erythrocytes and hamster and mouse lymphocytes. The agglutination was not inhibited by simple sugars. The major active component was purified and determined to be a beta-structure protein containing 2.7% glucose as sugar moiety. The molecular weight was estimated to be 12,000 by gel filtration and 13,000 by polyacrylamide gel electrophoresis, in the presence of sodium dodecylsulfate. Its isoelectric point was 6.1, and it contained high amounts of glycine, serine and threonine, but no half cystine or histidine. It had no subunit structure, and the C- and N-terminal amino acids were threonine and arginine, respectively.

ABO Blood-Group System↗
PubMed1978

Relations of pain threshold and pain tolerance in cold water with scores on Maudsley Personality Inventory and Manifest Anxiety Scale.

Testing 56 Japanese undergraduates, the relationships between pain threshold and pain tolerance in cold water and personality factors were investigated. Significant negative correlations of moderate magnitude between the pain threshold and scores on Maudsley Neuroticism and the Manifest Anxiety Scale were found. On the contrary, significant positive, moderate correlations between pain tolerance and the Maudsley Extraversion were obtained.

Cold Temperature↗
PubMed1977

Electrical activity of superior colliculus associated with eye movements in alert cats.

Eye movement potentials (EMPs) were recorded from the superior colliculus (SC) of awake cats. When cats moved the eyes in front of a patterned or diffuse field, the EMP appeared as a sequence of the three components; EMP1, EMP2 and EMP3. The EMP1-EMP2-complex occurred while the eyes were moving and it had a wave form similar to that of the flash-evoked response. The EMP3 was seen 30-50 msec after the end of eye movement. In dark only the EMP3 was observed; its delay after the end of eye movement was as large as 70 msec. By placing electrodes at various depths of SC, the EMP was found to originate in the superficial layers. In immobilized, encéphale isolé cats, placed in dark or made blind by enucleation, the followings were observed; 1) Passive movements of the eye did not give rise to any significant electrical activity of SC. 2) Spontaneous mass activity of the oculomotor nucleus, that would result in eye movements if the cats were not paralysed, was followed by sharp wave activity of SC and this was the same after the visual cortex had been ablated bilaterally. From these findings it is concluded for the EMPs of awake cats that the EMP1-EMP2-complex is a response to retinal impulses caused by movements of images, whereas the EMP3 is due to an extraretinal factor such as the corollary mechanism. Besides the EMPs as described above, some activity was found to occur about 150 msec before the onset of eye movement. This EMP, called early EMP, seemed to appear when it was followed by eye movements with relatively high speeds.

Action Potentials↗
PubMed1977

Threshold and reaction time to noxious stimulation: their relations with scores on Manifest Anxiety Scale and Maudsley Personality Inventory.

With 20 male Japanese undergraduates relationships between electric shock threshold and reaction time to electric shock with 10 v and personality factors were investigated. Significnat product-moment correlations of --.79 and --.69 between response to noxious stimulation and Manifest Anxiety and Maudsley Neuroticism scores were found.

Differential Threshold↗