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Biomedical subjects

K Shimoda

Publications and source records attributed to K Shimoda.

At least 253 records · Page 14Linked to original sources
PubMed1981

Bacterial flora of ex-germfree mice after oral inoculation of feces from various species of conventional animals.

Emulsions of fresh feces from a conventional mouse, rabbit, dog or cynomolgus monkey were administered orally to germfree and conventional ICR mice. Various fecal floras bearing a striking resemblance in pattern to those in the inoculant became established in the inoculated ex-germfree mice. Reinoculation of these ex-germfree mice with fecal emulsions from a conventional mouse made 21 days after the original inoculation (conventionalization) led to alteration of their floras to the indigenous pattern of conventional mice. The administration of feces from the conventional animals of different species did not appreciably modify the fecal flora of conventional mice. The results indicate that intestinal flora of animals is determined in the first stage by that of the contaminants, but this would be altered later under the predominant influence of the various host factors.

Animals↗
PubMed1981

Enterobacter cloacae, Serratia marcescens and Yersinia enterocolitica in colonies of laboratory mice, rats and rabbits: an attempt of isolation.

An attempt to isolate Enterobacter cloacae, Serratia marcescens and Yersinia enterocolitica was made in a total of 931 laboratory animals (mice, rats and rabbits) from five representative commercial breeders in this country and from our laboratory colonies. E. cloacae organisms were isolated from feces and other specimens of 15 mice and 17 rats between November 1979 and July 1980. Neither S. marcescens nor Y. enterocolitica was detectable in any specimen of feces, fur and skin, nasal discharge, lungs, urinary bladder or urine from the animals examined.

Animals↗
PubMed1980

Enhanced production of virus-inhibiting factor (interferon) in human diploid cells by ultraviolet irradiation and temperature shift-down after stimulation with Newcastle disease virus.

The production of the virus-inhibiting factor or interferon (IF) was highest in cells incubated at 37 C after inoculation with Newcastle disease (ND) virus and decreased as the incubation temperature was lowered. Shift-down of incubation temperature to 32 C or 34 C after incubation at 37 C for 4--7 hr enhanced IF production in cell cultures stimulated with ND virus, as compared with cultures incubated continuously at 37 C. Shift-down to 32 C after incubation at 37 C for 6 hr was optimal for this enhancement of IF yield. Enhanced IF production was also observed in cell cultures irradiated by ultraviolet light 4--7 hr after stimulation with ND virus.

Cell Line↗
PubMed1980

Colonization and pathogenicity of Saccharomyces cerevisiae, MC16, in mice and cynomolgus monkeys after oral and intravenous administration.

Saccharomyces cerevisiae, an ascomycetous yeast and a candidate for a host-vector system in recombinant DNA experiments, was examined for its pathogenicity and colonization in mice and cynomolgus monkeys as the models of the biological containment level. Adult mice given perorally with 5.5 or 2.4X10(7) cells of MC16 strain S. cerevisiae excluded them rapidly and no colonization of the cells in the abdominal organs, lymph nodes or gastrointestinal wall was demonstrated. No change in the fecal flora was observed. Cynomolgus monkeys after peroral administration of 4.9X10(7) or 7.8X10(8) cells showed a similar tendency of rapid exclusion and the lack of colonization. Cortisone acetate treatment had no significant effect. Intravenous administration of 3.9X10(7) yeast cells had no pathogenic effect and no viable yeast cell was detected in the blood. The biological containment level of S. cerevisiae was suggested to be not lower than that of E. coli K12 B1 level, and the possibility of achieving the B2 level was also suggested.

Administration, Oral↗
PubMedDate not supplied

Administration of chlordiazepoxide affects [3H][3-MeHis2]thyrotropin-releasing hormone binding in rat brain.

The effects of acute and subchronic administration of chlordiazepoxide (CDZ) on [3H][3-methyl-histidyl2]thyrotropin-releasing hormone binding to thyrotropin-releasing hormone (TRH) receptors in membrane preparations from various regions of rat brain were examined. Acute administration of CDZ (50 mg/kg x 3 within 24 h) did not alter either the equilibrium dissociation constant (Kd) or the maximum number of binding sites (Bmax) in cerebellum (CB), olfactory bulbs (OB), frontal cortex (Cx), hypothalamus (HT) or corpus striatum (ST). However, the Kds of the pyriform cortex/amygdala (PC/A) (Kd = 3.6 +/- 0.1 nM compared to 1.9 +/- 0.1 nM in the control group; p less than 0.01) and the hippocampus (HP) (Kd = 7.8 +/- 0.7 nM compared to 2.1 +/- 0.1 nM in the control group; p less than 0.01) were increased. There were no changes in Bmax. Subchronic administration of CDZ (50 mg/kg/day for 7 days) increased the Kd of the PC/A complex (p less than 0.05), the OB (p less than 0.05) and the HP (p less than 0.01) without altering in Bmax. These results, showing regional differences in the response of TRH receptors to acute and subchronic CDZ administration, suggest that reduced affinity of TRH receptors in the PC/A complex, OB and HP may be related to some of the neurobiological actions of CDZ and/or its metabolites.

Animals↗
PubMedDate not supplied

Effects of transforming growth factors on dopaminergic neurons in culture.

It was recently reported that TGF-beta 1 either had no significant effect on or increased the survival of dopaminergic neurons in culture. TGF-beta 2 and TGF-beta 3 were reported to cause increased survival or to greatly inhibit survival. The transforming growth factors are a highly pleiotropic group of compounds, and the above results suggest that their actions may be critically dependent on the conditions of the assay. We have therefore tested these compounds under optimal conditions of culture, in a medium containing a low (2.5%) concentration of fetal bovine serum. TGF-beta 2 and 3 inhibited neuronal (MAP2-pos) survival only at the highest concentration (10 ng/ml) tested, while inhibition of survival of dopaminergic neurons was observed at 1.0 and 10 ng/ml. These results therefore suggest that the inhibitory action of TGF-beta 2 and 3 on the survival of dopaminergic neurons in culture, under the experimental conditions outlined, may be relatively specific.

Animals↗
PubMedDate not supplied

Sexual dysfunction following stroke.

This study was undertaken to examine the prevalence of sexual dysfunction among 100 patients (75 men and 25 women) following stroke and its relationship to neuropsychiatric impairments or stroke characteristics. Forty-four men (58.6%) and 11 women (44.0%) reported dissatisfaction with their sexual functioning after stroke, as compared with only 16 men (21.3%) and five women (20.0%) before stroke. Twenty men (26.6%) and six women (24.0%) reported diminished libido after stroke as compared with eight men (10.6%) and seven women (28.0%) before stroke. Patients with sexual dysfunction had significantly more depressive symptoms among both males (P =.007) and females (P =.0005) and more impaired activities of daily living (ADL) in males (P =.0009). Based on logistic regression, the independent predictors of poststroke sexual dysfunction were Hamilton Rating Scale for Depression (HAM-D) score (odds ratio [OR] 1.55; 95% confidence interval [CI], 1.20 to 2.01), left hemisphere lesion (OR, 16.51; 95% CI, 2.34 to 116.25), and poststroke depression (OR, 8.09; 95% CI, 1.28 to 51.38). These data suggest that treatment of depression may have a significant beneficial effect on patients with sexual dysfunction. Our findings also suggest that left hemisphere lesions, for unknown reasons, play an important role in poststroke sexual dysfunction.

Activities of Daily Living↗