[Morphological and immunohistochemical investigation of solitary lymphatic nodules in the normal human terminal ileum and duodenal bulb].
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Duodenum↗
Biomedical subjects
K Shimada
Publications and source records attributed to K Shimada.
[Clinical effect of UFT on prostatic cancer].
A phase II study of UFT, a mixture of futraful and uracil in a rate of 1:4, was performed for prostatic cancer in 5 cooperative institutions. UFT was orally administered at a daily dose of 600 mg (t.i.d.) for 4 weeks. Twenty-two patients treated with UFT were evaluated according to Koyama-Saito's criteria. Tumor staging determined by transrectal ultrasonography and other methods revealed stage B in 4 patients, stage C in 7, and stage D in 11. The overall response rate was 18.2%. Complete response was obtained in 1 patient with stage B disease, partial response in 3, minor response in 1, no change in 15, and progressive disease in 2. With respect to toxicity, anorexia was observed in 9 patients, nausea and vomiting in 8, stomatitis in 4, and diarrhea in 3. White blood cell count was less than 3,000 cells per mm3 in 1 patient, and in another case, hepatic function disorder was observed. From the results obtained, the use of UFT is TS considered desirable for prostatic cancer.
Adenocarcinoma↗
[Clinical statistics on outpatients, inpatients and operations in 1983].
Statistical studies on 1,990 outpatients, 574 inpatients and 606 operative procedures at our department in 1983 revealed the following. The most frequent diseases among the outpatients were urogenital infections followed by anomalies, tumors and stones. The major diseases among the inpatients were hypospadias, vesicoureteral reflux, congenital urethral stenosis, benign prostatic hypertrophy and bladder tumor. A total of 606 operations were performed on 559 patients, and the five major operations were hypospadias repair (80), TUR-P (54), optic internal urethrotomy (51), ureterocystoneostomy (40) and orchidopexy (34).
Adolescent↗
[A case of multiple extracranial meningiomas].
A case of multiple extracranial meningiomas, occurring in the orbita, both ethmoidal sinuses and the frontal sinus of a 60-year-old man, is reported. These meningiomas were histologically similar and diagnosed as transitional type benign meningioma. On whole body examinations including CT scans, we could not find any other primary foci. There fore, these meningiomas were thought to be a case of multiple extracranial meningiomas occurring at the same time. As for multiple extracranial meningiomas, this is the first case report in Japan. In general, extracranial meningiomas have been thought to be derived from ectopic arachnoid cell rests. Our case will be extremely important in the light of the histogenesis of extracranial meningiomas.
Ethmoid Sinus↗
[Study on the function of the sphincter of Oddi in patients with gallstone disease by dynamic hepatobiliary scintigraphy].
By using hepatobiliary scintigraphy, function of the sphincter of Oddi in 88 patients undergoing cholecystectomy with or without common bile duct exploration and T-tube drainage for gallstone disease was studied. These patients consisted of three groups; 57 patients with gallbladder stones (Group I), 25 with common duct stones migrated from the gallbladder (Group II) and 6 with primary common duct stones (Group III). Time-activity curves (T-A curve) obtained from the region of terminal end of the common bile duct were classified into 4 types which were different from each other. On the T-A curve, the rate of decrease (ROD, %) was defined as the difference between the count rate in the maximum and that at the 20 min after the maximum. The ROD in Group I was significantly greater than that in Group II or III (p less than 0.01). The ROD in Group II was also significantly greater than that in Group III (p less than 0.05). The increase of ROD in Group III after cholecystokinin-pancreozymin administration was less remarkable as compared with that in Group II. Thus, we conclude that the function of the sphincter of Oddi in these three groups may be different from each other.
Ampulla of Vater↗
Cloning and sequence analysis of cDNA for human prealbumin.
A cDNA coding for human prealbumin has been cloned from a cDNA library prepared from human liver. The DNA sequence codes for a polypeptide which consists of 147 amino acids including a whole human prealbumin sequence and a putative signal sequence. The elucidation of this prealbumin cDNA sequence is expected to facilitate a prenatal diagnosis of familial amyloidotic polyneuropathy.
Amino Acid Sequence↗
Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family.
We reported that several DNA sequences homologous to mitochondrial DNA (mtDNA) are present in the human nuclear genome (Tsuzuki et al. (1983) Gene 25, 223-229). Detailed Southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated DNA sequences of the KpnI 1.8 kb family. Nucleotide sequence analysis showed that the KpnI 1.8 kb DNA is flanked with imperfect 15-base pair (bp) direct repeats of mtDNA. This KpnI 1.8 kb DNA has an A-rich sequence at its 3'-end, and has a considerable homology with one of the published cDNA sequences homologous to one of the human KpnI families and also to one of the African green monkey KpnI families, KpnI-LS1. These structural features suggest that the KpnI 1.8 kb DNA is a movable element and is inserted within the mtDNA-like sequence by an RNA-mediated process.
Animals↗
Studies on steroids. CC. Determination of 17-ketosteroid sulphates in serum by high-performance liquid chromatography with electrochemical detection using pre-column derivatization.
A new sensitive method is described for the determination of 17-ketosteroid sulphates, particularly dehydroepiandrosterone sulphate, in human serum by high-performance liquid chromatography with electrochemical detection. The 17-ketosteroid sulphates in serum were extracted with acetonitrile and derivatized with p- nitrophenylhydrazine in trichloroacetic acid--benzene solution. The p- nitrophenylhydrazones were separated by high-performance liquid chromatography on a mu Bondapak C18 column using methanol--0.5% ammonium dihydrogen phosphate (8:3) as a mobile phase. The proposed method proved to be applicable to the quantitation of 17-ketosteroid sulphates with satisfactory sensitivity and reliability, providing a quantitation limit of 80 ng/ml and coefficient of variation of 4%. A good correlation was observed between the values obtained by the present method and radioimmunoassay for dehydroepiandrosterone sulphate in serum.
Adult↗
Development of signs and symptoms of congenital choledochal dilatation: its relation to pregnancy with special reference to experimental study in guinea pigs.
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Adult↗
Fluorometric high-performance liquid chromatography of 9-aminophenanthrene-derivatized free fatty acids.
The application of 9-aminophenanthrene (9-AP), a fluorescence-labeling reagent for free fatty acids (FFA), was examined. 9-AP dissolved in benzene was added to a benzene solution of FFA chlorides derived from FFA and oxalyl chloride. The mixture was allowed to react for 45 min at 70 degrees C. By the method, 9-AP-tagged FFA with a strong fluorescence was formed. The materials thus obtained have a lambda max at around 303 nm for excitation and 376 nm for emission. By using this derivatization method, recoveries were measured for seven kinds of FFA added to 0.5 ml of healthy human serum. Significant recoveries ranging from 96 to 107% (coefficient of variation 1.4-5.0%) were obtained for each FFA. The proposed method was clinically applied to the determination of FFA in 0.5 ml of healthy human serum, and almost satisfactory results were obtained. Detection limits of FFA by this derivatization method were 10 pmol for C14:0, C16:0, C16:1, C18:1 and C18:2, and 15 pmol for C18:0 and C20:4. As a quantitative measurement of FFA, gas chromatography and high-performance liquid chromatography with fluorescence detection, which have been routinely used, were chosen for comparison with the present method.
Chromatography, High Pressure Liquid↗
Determination of a new antihypertensive agent (2R,4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4- thiazolidinecarboxylic acid in blood by high-performance liquid chromatography with electrochemical detection.
A sensitive method has been developed for the determination of a new antihypertensive agent, (2R,4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4-thiazolidinecar boxylic acid (I), in human blood. Because the drug is unstable in biological fluids, it was immediately derivatized by treating the freshly drawn blood specimens with N-(4-anilinophenyl)maleimide. The adduct was separated and determined by high-performance liquid chromatography with electrochemical detection on a reverse-phase column. The assay method was satisfactory with respect to the sensitivity and precision, providing a quantitation limit of 2 ng/ml and coefficient of variation of 3%. Preliminary pharmacokinetic data were obtained by orally administering I to two patients with essential hypertension.
3-Mercaptopropionic Acid↗
Isolation and characterization of phage clones carrying the human argininosuccinate synthetase-like genes.
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Argininosuccinate Synthase↗
Low molecular weight-IgM antibody in syphilis detected by Treponema pallidum immune adherence (TPIA) test.
Sera obtained from patients with syphilis were subjected to gel filtration chromatography to estimate antibody activity in the fractions by means of Treponema palludum immune adherence (TPIA) test. Antibody activity was found in IgM and IgG fractions and in the intermediate fraction between them. The TPIA activity of the intermediate fraction was depleted by an immunosorbent coupled with anti-mu chain antibody but not by that coupled with anti-alpha chain antibody. After separation by gel filtration and affinity chromatography, the fraction with the TPIA activity showed a positive precipitation reaction against anti-mu chain antiserum but a negative reaction with anti-alpha chain or anti-gamma chain antiserum. The antibody activity in this fraction was thus confirmed to be mainly due to low molecular weight-IgM antibody. In untreated patients with primary and secondary syphilis, the TPIA activity was demonstrated with high frequency in the low molecular weight-IgM fraction and it decreased gradually after treatment, although lowered activity persisted over a long period of time. Late syphilis cases did not show any appreciable change in TPIA activity in this fraction even after treatment.
Antibodies, Bacterial↗
Masticatory muscle activities during rhythmic jaw movement associated with tooth contact in lightly anesthetized rats.
Rhythmic jaw movements were observed in lightly anesthetized rats when a bitable object was placed between the maxillary and mandibular incisors. To elicit the jaw movements, an initiating stimulus was required. A successful stimulus was to push on the mandible at the incisors in both opening and posterior directions. This may be a suitable movement for studying mastication because of the following properties: (i) The rhythmic jaw movements could not continue without tooth contact. (ii) The timing pattern of the electromyographic activity of the masseter and digastric muscles and the tooth contact was similar to that of human mastication. (iii) Frequency of the movements was independent of hardness of the object placed between the incisors, though oral stimulation easily stopped the movement as did a noxious stimulus applied around the oral cavity. We conclude that peripheral activation, which may be generated by tooth contact, is as essential to the rhythmic jaw movement as central activation.
Anesthesia, General↗
Preparation of specific antisera to estrone-3-glucuronide and estriol-3-glucuronide.
The preparation and antigenic properties of estrone-3-glucuronide- and estriol-3-glucuronide-bovine serum albumin conjugates in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed high specificity to estrone-3-glucuronide and estriol-3-glucuronide, respectively, exhibiting little cross-reactivities with other estrogen conjugates and no cross-reactions with related steroids except for free estrogens, their 3-methyl ethers and 3-sulfates. The cross-reactive antibodies were eliminated by partial immunoadsorption on affinity chromatographic media using the estrone-3-methyl ether 17-(O-carboxymethyl)oxime- and estriol-3-methyl ether 16 (or 17)-hemisuccinate-aminohexyl Sepharose conjugates, respectively. The purified antisera exhibited no cross-reactivities with free estrogens and ring A conjugates of estrone and estriol.
Animals↗
In vitro metabolic conjugation of catechol estrogens.
In vitro metabolic conjugation of the catechol estrogens, 2-hydroxyestrone and 4-hydroxyestrone, has been investigated by means of HPLC with electrochemical detection. Sulfation of 2-hydroxyestrone and 4-hydroxyestrone with the rat liver 105 000 g supernatant fortified with 3'-phosphoadenosine-5'-phosphosulfate provided the 2- and 4-monosulfates, respectively. Glucuronidation of the two catechols with the rat and human liver 1500 g supernatant in the presence of uridine-5'- phosphoglucuronic acid gave the 2- and 4-glucuronides, respectively. In contrast, incubation with the guinea pig liver 1500 g supernatant yielded both isomeric monoglucuronides . When 2'-hydroxyestrone was incubated with rat liver 1500 g supernatant and S-adenosyl-L-methionine, the 2- and 3-monomethyl ethers were formed in an equal amount, while 4-hydroxyestrone was transformed into the 4-methyl ether in 12 times greater yield than the 3-methyl ether. The participation of sulfation and glucuronidation in the formation of guaiacol estrogens is discussed.
Animals↗
Preparation of specific antiserum to estriol 3-sulfate 16-glucuronide.
The preparation and antigenic properties of estriol 3-sulfate 16-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Coupling of 6-oxoestriol 3-sulfate 16-glucuronide acetate-methyl ester 6-(O-carboxymethyl)oxime with BSA by the activated ester method followed by removal of the protecting groups with alkali provided the desired conjugate. The antisera raised against the conjugate in rabbits were highly specific to the double conjugate, estriol 3-sulfate 16-glucuronide, discriminating from ring A or D monoconjugated and unconjugated estrogens. The specificity of antisera elicited has been discussed on the basis of stereochemistry of the hapten-[C-6]-BSA conjugate.
Cross Reactions↗
Partial structure of panaxan A, a hypoglycaemic glycan of Panax ginseng roots.
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Chemical Phenomena↗