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Biomedical subjects

K Shimada

Publications and source records attributed to K Shimada.

At least 1,315 records · Page 73Linked to original sources
PubMed1986

[Percutaneous subacute toxicity study of 10% nitroglycerin (NT-1 ointment) in rabbits].

NT-1 ointment is a compound containing 10% nitroglycerin which is topically applied to the skin for angina pectoris. The subacute toxicity of this compound was examined by the continuous application for 5 weeks to the shaven skin of rabbit back at dose levels of 240, 120 and 60 mg/kg, and the recovery was examined 3 weeks after withdrawal of the drug. No special skin response to the NT-1 ointment was observed other than an erythema of the grading 2-3 Draize points, while the control ointment, which was the base ointment without nitroglycerin, showed a higher grade of erythema equivalent to 4 Draize point with crusta formation. No relationship between the dose level of NT-1 ointment and the skin response was observed, and skin response was thought to be caused mainly by the base ointment. The skin response gradually reduced even during the application period, and the skin response disappeared within a few days after withdrawal of the ointment. Histological examination of the treated skin at the respective ends of the application and recovery periods showed thickening of the corneum and epithelial layer, round cell infiltration and fibrosis in the corium, and development of hair folliculi in the subdermis. No systemic effect of the topically applied NT-1 ointment was observed in the majority of the animals, either in behavior, hematologic and electrocardiographic examination, food consumption, body weight change or urinalysis.

Administration, Cutaneous↗
PubMed1986

Gizzard motility in relation to oviposition in the chicken.

Changes in gizzard motility were studied with a particular reference to egg laying by a radiotelemetric measurement of electrical activity of the thick caudodorsal muscle of the gizzard in the chicken. There was a clear diurnal pattern in gizzard motility. Superimposed on this diurnal pattern, gizzard motility declined shortly before oviposition followed by an immediate increase after oviposition. Although fasting reduced mean frequencies of gizzard motility on an hourly or daily basis, a marked increase still occurred soon after oviposition. These results suggest that gizzard motility is not only influenced by food availability but also is affected by the hen's laying behavior.

Animals↗
PubMed1986

[Malignant paraganglioma of the peri-iliac artery].

A malignant nonfunctioning paraganglioma of the pelvic retroperitoneum (left external iliac artery) with metastases to the femoral lymph nodes was found in a 68-year-old woman at exploratory laparotomy. Light microscopy showed a typical alveolar pattern with fine vascular stroma. The tumor cells had finely granular eosinophilic cytoplasm and oval to round nuclei with pleomorphism and mitotic figures, Grimelius stain showed no argyrophilic granules, but S 100 protein was demonstrated in the cytoplasm by the PAP method of Sternberger. Formaling fixed tissues were examination by electron microscopy, and dense core granules consistent with neurosecretory granules were found in the cytoplasm of some tumor cells.

Aged↗
PubMed1986

[A case of fibrous hamartoma of infancy and a review of the Japanese literature].

A case of fibrous hamartoma of infancy in the left axilla of an 18-month-old boy, is reported with some discussion (citations of references). The tumor was situated in the subcutaneous fatty tissue, and its border was unclear. It was removed easily and found to measure 2.5 X 1.5 X 1.5 cm. Histologically, in addition to the three main components, i.e., trabeculae of fibrous tissue, immature mesenchymal cells with a mucoid matrix and interspersed mature fat, a capillary vasoformative proliferation, peripheral nerve fibers and lymph follicle-like structures mingling with and transforming into one another were noted throughout the tumor. On the basis of these findings, immature mesenchymal cells were considered as having very important roles with regard to the histogenesis of fibrous hamartoma of infancy.

Adipose Tissue↗
PubMed1986

[Experience in transurethral prostatic resection by the Mauermayer method].

One hundred patients with benign prostatic hypertrophy were treated by transurethral prostatic resection by the Mauermayer method. Average resection time was 67.5 minutes and average weight of resected tissue was 26.2 g. Incidence of intraoperative and postoperative complications were within reasonable range and no serious complication including death occurred. Preoperative and postoperative uroflowmetric examinations were carried out on 61 patients and a significant improvement of urinary flow was demonstrated. The Mauermayer method is a safe and effective method of prostatic resection.

Aged↗
PubMed1986

Structure and expression of the mutant prealbumin gene associated with familial amyloidotic polyneuropathy.

We have cloned a chromosomal DNA segment that covers the entire sequence for the mutant prealbumin gene associated with familial amyloidotic polyneuropathy, and determined the sequence of the gene including 581 base-pairs of the 5'-flanking region and 95 base-pairs of the 3'-flanking region, except for the internal portions of the second and third introns. This sequence was aligned with that of the previously determined normal prealbumin gene and both sequences matched perfectly except for a single-base substitution present in the second exon. This substitution is responsible for the transition from valine (GTG) to methionine (ATG) in the mutant prealbumin and creates NsiI and BalI restriction sites in the mutant prealbumin gene. We also analysed prealbumin mRNAs in the livers of a disease-free individual and one with familial amyloidotic polyneuropathy and confirmed that there is no difference in the levels and sizes of the prealbumin mRNAs between the two. As we have demonstrated already that individuals with familial amyloidotic polyneuropathy are heterozygous for the prealbumin gene, carrying one normal and one mutant gene, levels of the two different mRNAs within the liver of an individual with familial amyloidotic polyneuropathy were separately estimated and were demonstrated to be approximately equal.

Amyloidosis↗
PubMed1986

Messenger RNA coding for argininosuccinate synthetase in citrullinemia.

Messenger RNA coding for argininosuccinate synthetase (ASS), extracted from the livers of some patients with citrullinemia, was analyzed using a cell-free translation system and dot and Northern blot hybridization with cDNA probe for ASS. In patients with quantitative-type citrullinemia, called type II here, previous studies have demonstrated that the hepatic content of the enzyme was about 10% of the control value, whereas the translatable mRNA level for the enzyme was similar to that of control livers. Here, we confirmed that the type II liver contained an almost normal amount of mRNA coding for ASS, judged by the dot-blot hybridization technique with cDNA. Northern blot hybridization of RNA indicated that there was hybridizable mRNA of approximately normal size (about 1.7 kilobase [kb]) in each, suggesting that large structural gene deletions had not occurred. These results indicate that in type II citrullinemia, the decrease in the enzyme protein is due either to increased degradation of the enzyme or to decreased or inhibited translation in the liver. Another type of citrullinemia was found and classified as type III. It is characterized by no detectable enzyme activity for ASS or translation activity for ASS mRNA. However, a smaller amount of RNA molecule hybridized for ASS cDNA was detected.

Adult↗