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Biomedical subjects

K Seidel

Publications and source records attributed to K Seidel.

At least 73 records · Page 4Linked to original sources

[Use of UV rays for the disinfection of water. III. UV sensitivity of Legionella pneumophila of different ages in cold and warm drinking water].

In drinking water the sensitivity of L. pneumophila serogroup 1 type Philadelphia and L. pneumophila serogroup 5 type Dallas were studied with a flowthrough u.v. light treatment apparatus. By washing purified cells from broth cultures were used as inoculum directly ( = young cultures) or kept 2-3 weeks in drinking water in the dark ( = old cultures). A decrease of 99,9999% was found after u.v. treatment by 19 mWs/cm2 for young cultures and by 15 mWs/cm2 for old cultures. Reductions of 99,9999% were obtained by 16 mWs/cm2 in cold drinking water (13-16 degrees C) and by 13 mWs/cm2 in warm drinking water (45-47 degrees C). L. pneumophila serogroup 1 and L. pneumophila serogroup 5 show a very similar susceptibility to u.v.-irradiation. Reductions of 99,9999% were obtained by 14 mWs/cm2 and 15 mWs/cm2, respectively. Thus L. pneumophila-suspensions proved to be more sensitive to u.v.-irradiation than E. coli oder E. faecium in earlier experiments.

Age Factors↗

Nosocomial legionella pneumonia: demonstration of potable water as the source of infection.

From January 1983 until December 1985, 35 cases of sporadic nosocomial legionella pneumonia, all caused by Legionella pneumophila, were diagnosed in a university hospital. L. pneumophila serogroup (SG) 1 was cultured from 12 of the 35 cases and compared to corresponding L. pneumophila SG 1 isolates from water outlets in the patients' immediate environment by subtyping with monoclonal antibodies. The corresponding environmental isolates were identical to 9 out of 12 (75%) of those from the cases. However, even in the remaining three cases identical subtypes were found distributed throughout the hospital water supply. From the hospital water supply four different subtypes of L. pneumophila SG 1 were isolated, three of which were implicated in legionella pneumonia. Of 453 water samples taken during the study 298 (65.8%) were positive for legionellae. Species of Legionella other than L. pneumophila have not been isolated. This may explain the exclusiveness of L. pneumophila as the legionella pneumonia-causing agent. Our results suggest that the water supply system was the source of infection.

Antibodies, Monoclonal↗

Lack of an effect of dietary fructose on severity of zinc deficiency in rats.

Because feeding rats diets containing fructose as the carbohydrate source reduces copper and selenium status, we investigated whether the type of dietary carbohydrate also affected indices of zinc status. The experimental design was a 2 X 2 factorial study with the source of dietary carbohydrate (cornstarch or fructose) and the level of dietary zinc (0.7 or 31 micrograms Zn/g) as the variables. The experiment utilized 76 weanling male Sprague-Dawley rats randomly assigned to one of four dietary groups. Animals fed a zinc-deficient fructose diet were allowed to consume the diet ad libitum; all other groups were pair-fed to that group to ensure equivalent nutrient and energy intake. The results of the 29-d study showed that the most sensitive indices of zinc status measured, including growth, survival and the zinc concentrations of plasma, femur and testes, were not affected by the type of dietary carbohydrate. This lack of an effect of fructose on the zinc status of the experimental animals indicates that the ability of fructose to exacerbate copper and selenium deficiencies is specific, rather than representing a generalized effect of this simple sugar on the requirements and/or metabolism of all essential trace elements.

Animals↗

Effect of heparin and citrate on measured concentrations of various analytes in plasma.

Frequently it is assumed that concentrations of biochemical analytes are similar for serum and plasma, without regard to the anticoagulant. Recently it has been hypothesized that use of certain anticoagulants results in an osmotic redistribution of fluid between blood cells and plasma, causing some dilution of the plasma. We sought to determine the effect of two commonly used anticoagulants, heparin and citrate, on the measured concentrations of 13 clinical-chemical analytes, including selected trace elements, vitamins, lipids, and proteins. The data demonstrate that hyperosmolar citrate causes a significant dilution of all indices measured. In contrast, heparin had no osmotic effect; concentrations of most of the analytes measured in the heparinized plasma were statistically no different from those measured in the corresponding serum. Therefore, anticoagulants must be chosen carefully, especially if concentrations in plasma and serum are to be compared.

Blood Chemical Analysis↗

[Disinfection of sewage sludge by aerobic thermophilic treatment].

Thermal stabilization of sewage sludge, performed in a pilot plant under aerobic conditions, resulted in microbiological and virological decontamination. A minimum temperature of 50 degrees C was necessary to inactivate coliform bacteria, E. coli and Salmonella sp. The rate of inactivation of these bacteria and enteric viruses was dependent on the concentration of microbes in the raw sludge, the temperature and the detention time of the sludge. At a temperature of 56 +/- 1 degree C, which was achieved by autogeneous heating of the processed sludge, pathogens were usually inactivated after two hours, but the time required for inactivation was shorter when the temperature was higher.

Aerobiosis↗

Rheographic indications for reduced cerebral vasoconstriction after oral magnesium medication in tetanic patients, a double-blind, placebo-controlled trial.

Volume and tonus oscillations of cerebral blood vessels are measurable with rheoencephalography. A lowered amplitude under resting conditions and an increased reactivity after hyperventilation (with pronounced and prolonged vasoconstriction) was found in tetanic patients, 360 mg Mg/day (as magnesium citrate tablets) was administered in 17 tetanic patients (A) and a placebo in 18 patients (B) in a double-blind, placebo-controlled trial. After 4 weeks the hyperventilation-induced vasoconstriction was reduced significantly within group A compared to group B.

Adolescent↗

The hyperventilation syndrome: a neurosis or a manifestation of magnesium imbalance?

It has been proven with clinical, psychological and electromyographic tests that the hyperventilation (HV) syndrome cannot be separated from so-called genuine tetany. Tetanic patients with and without HV are characterized by a significant hypocalcemia; but a significant hypomagnesemia is exclusively found in tetanic patients with HV attacks. Possible pathogenic mechanisms are discussed which may explain the HV attacks as a consequence of primary magnesium deficiency.

Adolescent↗

[Unusual variations of the large arteries of the thigh].

Three rare variations of the large arteries of the thigh are known: Hypoplasia or aplasia of the superficial femoral artery in combination with persistent sciatic artery which normally is present during embryogenesis for a few days only. Typical finding is a large dorsal artery in the thigh accompanied with a hypoplastic superficial femoral artery. For the first time angiography of this vascular situation is shown in two projections. Complications of persistent sciatic artery include aneurysm (25%) and occlusion (embolic or thrombotic). Duplication of the superficial femoral artery. Absence or duplication of the deep femoral artery caused by separately originating branches. Knowledge of embryogenesis of the vessels helps to understand these artery variations.

Adult↗