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Biomedical subjects

K Satake

Publications and source records attributed to K Satake.

At least 163 records · Page 9Linked to original sources
PubMed1984

Experimental pancreatic carcinoma as a model of human pancreatic carcinoma.

Experimental pancreatic carcinoma induced by BHP in hamsters and by DMBA in rats are compared with human pancreatic carcinoma. Tubular adenocarcinoma and papillary adenocarcinoma in human pancreas and hamster pancreas appeared to be similar. However, poorly differentiated adenocarcinoma and acinar cell carcinoma were not observed in the experimental hamster model. Poorly differentiated adenocarcinoma and acinar cell carcinoma in human and rat pancreas were similar but experimentally induced papillary adenocarcinoma and cystoadenocarcinoma were not observed in the rat. Mucin producing cells were usually observed in human and experimental pancreatic carcinoma in the hamster but were rarely noted in the rat. These results indicate that experimental pancreatic carcinoma either in rats or hamsters is a good model for the understanding of both the histogenesis and the carcinogenesis of human pancreatic carcinoma.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1984

"Idiopathic" duodenal obstruction due to chronic pancreatitis.

Three cases of idiopathic duodenal obstruction due to chronic pancreatitis are described. Each received a different surgical treatment. One had a pancreatoduodenectomy because of the difficulty in distinguishing the benign mass from a malignant tumor, one had a cholecystectomy with conservative treatment and the third had a subtotal gastrectomy with a Billroth II anastomosis. All patients were free of symptoms from 1 to 4 years postoperatively. It is very important to establish the correct diagnosis before or during operation in the management of these patients.

Adult↗
PubMed1983

Transabdominal oesophageal transection for oesophageal varices: experience in 101 patients.

The results of transabdominal oesophageal transection in 101 patients with oesophageal varices are reported. The primary diagnosis was liver cirrhosis and 65 patients, idiopathic portal hypertension in 29 and extrahepatic portal obstruction in 7. Twelve patients died postoperatively. The mortality rate for emergency operations was higher than that for elective and prophylactic procedures. The majority of patients died from hepatic failure. Improvement, judged by radiographic and endoscopic examination, was 100 per cent and 96 per cent, respectively. Post-transection rebleeding was observed in 14 of 89 patients during the follow-up period of 1-13 years. There were 25 late deaths, caused mainly by hepatic failure. The 5-year survival rate, including postoperative deaths, was 72 per cent in idiopathic portal hypertension and 55 per cent in liver cirrhosis. We suggest that transabdominal oesophageal transection is an acceptable operative procedure for oesophageal varices.

Adolescent↗
PubMed1983

Long-term function of duct-ligated and free-duct whole pancreas transplants.

This report compares endocrine and exocrine responses of "free-duct" and "duct-ligated" whole pancreas isografts in Lewis diabetic rats during a 1-year period of observation. Fasting blood sugar, serum amylase, serum insulin, pancreozymin-secretin test, and L-arginine tolerance test assays were performed prior to and at periodic intervals after transplantation. Histological examinations of transplanted and native pancreas were performed monthly on animals in each group. Whole pancreas transplantations led to immediate and sustained normalization of FBS and insulin levels. In the "duct-ligated" group there was a gradual but insignificant decrease of serum insulin levels after 9 months. Depressed serum amylase levels in diabetic rats were reversed in both groups. Isolated islet iso- and allografts also reversed depressed amylase levels. The results of pancreozymin-secretin tests in islet isografted animals supported the postulated trophic effect of endocrine pancreas on exocrine pancreas. Although both whole-pancreas transplant groups showed a normal response to GTT for 12 months after transplantation, the "duct-ligated" group showed a significantly lower response to L-arginine tolerance test than the "free duct" model as early as 4 months after transplantation, suggesting a defect in B-cell function in long-term "duct-ligated" graft. Serial histological examinations of transplanted pancreases revealed more rapid atrophy and more intense fibrosis of acinar tissue in the "duct-ligated" group than in the "free duct" group. These results suggest that a "free duct" pancreatic isograft maintains a more stable endocrine function in a long-term study than does the "duct-ligated" pancreas, and that exocrine dysfunction in diabetic rats may be related to the endocrine disorder which can be reversed by both islet cell and whole-organ pancreatic transplantation.

Amylases↗
PubMed1983

Recirculation of indium-111-labeled lymphocytes in normal and allografted rats.

The kinetics of lymphocyte recirculation in normal and allografted rats with acute cardiac rejection was studied with indium-111 (In-111) labeled splenic lymphocytes in two groups of rats. Group 1 consisted of subgroups of normal Lewis rats infused with In-111 labeled unsensitized syngeneic cells (group 1a); ACI-sensitized syngeneic cells (group 1b); and ACI spleen cells (group 1c). Four rats from each subgroup were killed at 3, 6, 18, and 24 hr after cell infusion for blood, spleen, mesenteric lymph node (MLN), thymus, bone marrow (BM), liver, kidney, muscle, and heart scintillation counts. Group 2 consisted of Lewis recipients of ACI cardiac allografts infused with normal or with ACI-sensitized syngeneic splenic cells. Four rats from each subgroup were killed daily until rejection (day 7) for isotope counts of various organs. In ungrafted rats (group I), splenic accumulation of unsensitized syngeneic cells fell from 50% of the total injected dose/g tissue at 3 hr to 28% at 24 hr, whereas it rose from 12% at 3 hr to 39% at 24 hr in MLN. In contrast, the sensitized syngeneic and allogeneic cells homed preferentially to the spleen with insignificant accumulation in the MLN throughout the experiment. The BM and liver showed moderate accumulation while the thymus and nonlymphoid organs had low concentrations of labeled cells at all times. Splenic accumulation of unsensitized syngeneic cells in allografted rats (group II) showed a steep rise from day 1, reaching a peak at day 3, followed by a plateau--but sensitized cells demonstrated a peak on day 4 followed by a sharp decline until rejection. Accumulation of unsensitized cells in the MLN was significantly higher (P less than 0.001) than that of sensitized cells throughout the study. There was a significant fall (P less than 0.001) in radioactivity of BM, thymus, liver, and nonlymphoid organs from days 1-7, and the cardiac allograft demonstrated a reciprocal sharp rise in radioactivity. There was a significant early accumulation (P less than 0.001) of sensitized cells compared with unsensitized cells in the cardiac allograft on day 1. This study shows that In-111 labeled donor cells bearing surface antigen different from that of the recipient were sequestered from the circulating pool and immobilized in the spleen, but labeled donor cells with similar surface antigen to that of the recipient were recruited into the lymph node lymphocyte recirculating pool. It further demonstrates the difference in migration patterns of normal and sensitized syngeneic cells during acute allograft rejection.

Animals↗
PubMed1983

[The value of estimation of serum pancreatic elastase in experimental pancreatic disorders in rats].

The elastase levels in rat serum were measured in experimental pancreatic disorders by radioimmunoassay. After the induction of acute pancreatitis either by trypsin or normal saline, the serum elastase levels increased significantly. The serum elastase levels in trypsin induced pancreatitis were significantly higher than in normal saline induced pancreatitis. The levels in ascitic fluid were also higher in trypsin induced pancreatitis than in normal saline induced pancreatitis. Microscopically, haemorrhagic pancreatitis was found in trypsin induced pancreatitis and edemotous pancreatitis was found in normal saline induced pancreatitis. The serum elastase levels decreased significantly in rats who were treated by ethionine for long period. Microscopically, atrophic pancreas was found in those rats. These results suggest that the measurement of pancreatic elastase in either blood or ascitic fluid by radioimmunoassay is a useful diagnostic method for pancreatic disorders, especially in acute pancreatitis.

Acute Disease↗
PubMed1983

[Clinical studies of cefotiam in otorhinolaryngologic infections].

Cefotiam (CTM) was intravenously or intramuscularly given to 14 patients with infections in the field of otorhinolaryngology including 5 cases with chronic suppurative otitis media. Daily doses of CTM were 1 to 3 g and its administration period was 3 to 10 days. Clinical responses were excellent in 1 patient, good in 8, fair in 2 and poor in 3, the rate of satisfactory clinical responses (excellent and good) being 64.3% (9/14). Staphylococci were most frequently isolated from the clinical materials. As for side effects, elevation of GOT and GPT was observed in 1 patient.

Adult↗
PubMed1983

[Postprandial plasma secretin concentration and luminal pH in patients with gastrectomy].

The plasma secretin concentration in response to a meat meal and luminal pH were investigated in patients with gastrectomy: total gastrectomy with Roux-Y anastomosis or interposed jejunal anastomosis and partial gastrectomy with Billroth I or Billroth II anastomosis. The fasting plasma secretin concentration was significantly elevated in relation to the decreases of duodenal pH in postprandial state in normal subjects. No significant response of the plasma secretin and jejunal pH were observed in patients with total gastrectomy with Roux-Y anastomosis, however total gastrectomized patients with jejunal interposition showed the peak plasma secretin response in postprandial state. In patients with Billroth I the postprandial plasma secretin concentrations were well responded when the duodenal pH was below 4.5, however it was less than that of normal subjects. In patients with Billroth II the response of plasma secretin was much less than that of Billroth I, even though the decrease of jejunal pH was as comparable as in Billroth I. These studies suggest that a Billroth I operation after subtotal gastrectomy and interposed jejunal anastomosis after total gastrectomy are better procedures than the Billroth II or Roux-Y anastomoses to obtain endogenous secretin release.

Adult↗
PubMed1983

[Disseminated intravenous coagulation in acute necrotizing pancreatitis].

The coagulation changes observed in acute pancreatitis were studied clinically and those changes observed in acute experimental pancreatitis were compared with those after the intravenous infusion of pancreatic juice and ascitic fluid exudate obtained from bile-induced pancreatitis in dogs. The coagulation changes observed in six among 37 patients with acute pancreatitis and half of them died. Those changes observed clinically were either hypercoagulability or hypocoagulability. The coagulation changes after trypsin-induced acute experimental pancreatitis, elastase and autologous bile showed an indication of consumption coagulopathy. The effect upon blood coagulation after the intravenous injection of pancreatic juice included decreased platelet counts and plasma fibrinogen levels, prolonged partial thromboplastin and prothrombin time. The intravenous injection of pancreatic exudate produced greater changes than did those of an equal amounts of pancreatic juice. There was a shortening of E.L.T. and a marked increase in F.D.P. pancreatic exudate which accumulated during acute pancreatitis may contains a toxic substance or substances which contribute to the consumption of coagulation factors.

Acute Disease↗
PubMed1983

Postprandial plasma secretin response in patients following gastrectomy.

Endogenous secretin release and luminal pH changes after a test meal were investigated in five normal persons and in 22 patients after gastrectomy and various anatomic reconstructions, such as Billroth I and II anastomoses after subtotal gastrectomy and Roux-en-Y and interposed jejunal anastomosis after total gastrectomy. In patients in the Billroth I group, the response of postprandial secretin was good if the duodenal pH was below 4.5 but it was less than that of normal persons. After the Billroth II operation, even when the jejunal pH was below 4.0, the response was much less than that for the Billroth I group. After the complete abolishment of gastric acid secretion by total gastrectomy, no postprandial secretin response was noted in patients with the Roux-en-Y anastomosis. However, a response was seen in patients with interposed jejunal anastomosis, even though it was less than that for those in the Billroth I group and in normal persons. We conclude that the impairment of endogenous secretin release may be an important factor in pancreatic dysfunction after gastrectomy, and we suggest that a Billroth I operation after subtotal gastrectomy and interposed jejunal anastomosis after total gastrectomy are better procedures than the Billroth II or Roux-en-Y anastomoses to obtain endogenous secretin release.

Duodenum↗
PubMed1982

Radioimmunoreactive serum elastase levels and histologic changes during experimental pancreatitis in rats.

A radioimmunoassay system showed elastase levels of 128 to 232 ng/mL in normal rat serum. The average normal value was approximately 64-fold greater than the minimal detectable amount. No cross-immunoreactivity was found with human elastase 1, porcine elastase, and trypsin. The elastase and amylase levels in rat serum were measured during pancreatitis induced by trypsin (group 1) and normal saline solution (group 2). Within 15 minutes the serum elastase and amylase levels increased significantly and remained elevated. The serum elastase levels in group 1 were significantly higher than in group 2. Hemorrhagic pancreatitis was found in group 1 and edematous pancreatitis was found in group 2 five hours after induction of pancreatitis. Elastase levels in these tissues were significantly lower than those in normal tissue. The levels in ascitic fluid were higher than those found in serum.

Acute Disease↗
PubMed1982

Alpha-fetoprotein and carcinoembryonic antigen in pancreatobiliary disease with and without jaundice.

A comparative study of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) in serum was made by radioimmunoassay in 66 patients with pancreatobiliary disease with and without obstructive jaundice. biliary concentrations of these proteins were assayed in 12 patients with benign and 7 patients with malignant disease. There was no statistical difference in serum AFP between these two groups but there was a difference in serum CEA. Although CEA and AFP in bile in both groups were higher than in serum there was no statistical difference in serum CEA and AFP in patients with and without obstructive jaundice in either benign or malignant disease. In benign disease, patients with inflammation had higher serum CEA than normal. Although there was no statistical difference in serum CEA in patients with a without liver metastases, the serum CEA in liver metastases was significantly higher than in benign disease. We concluded that AFP is unlikely to be important in the assessment or management of patients with cancers other than those derived from the liver or yolk sac, and that the elevation of serum CEA in patients with malignant pancreatobiliary disease either with or without obstructive jaundice occurs mainly in the presence of widespread malignancy, especially in liver metastases.

Adult↗
PubMed1982

Serum elastase I levels in pancreatic disease.

The radioimmunoassay for human elastase I used in this study is accurate, sensitive, and specific, which we have confirmed. The assay can be done within 4 hours, which is important for clinical purposes. A total o 103 subjects were examined, and levels of 99 to 370 ng/dl (mean 200) in normal human sera were determined. The serum elastase levels in acute, acute relapsing, and chronic relapsing pancreatitis were significantly higher than normal. Although serial determinations returned to normal within 5 days after the onset of the attack, they decreased gradually and remained high on the 7th, 10th, and 11th days in patients who still had residual signs of pancreatitis. The values in patients with chronic pancreatitis and various other diseases were normal. The values in patients with acute pancreatitis were significantly higher than in those with hyperamylasemia of nonpancreatic origin. Twelve of 19 patients with pancreatic cancer had abnormal serum elastase levels; this was especially true in patients with cancer of the pancreatic head. We believe that the measurement of serum elastase levels by radioimmunoassay will become a useful diagnostic method for pancreatic disease in the future.

Amylases↗
PubMed1982

Cellular basis for accumulation of In-111-labeled leukocytes and platelets in rejecting cardiac allografts: concise communication.

Biodistribution and imaging studies in rats showed that In-111-labeled leukocytes and platelets accumulate progressively with time after transplantation in cardiac allografts undergoing rejection, but do not accumulate in normal syngeneic heart grafts. Maximum heart allograft-to-blood ratios of 9:1 were obtained, and allograft-to-native heart ratios of 17:1. Microscopic studies of the rejecting cardiac allografts showed that histologic findings paralleled the cellular changes predicted by the radionuclide studies. Intravenously administered Ga-67 citrate and Tc-99m sulfur colloid failed to show significant accumulation in rejecting grafts. The findings suggest that cellular rejection, rather than nonspecific inflammatory changes, is the primary basis for accumulation of In-111 leukocytes and platelets in rejecting cardiac allografts.

Animals↗