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Biomedical subjects

K Petzoldt

Publications and source records attributed to K Petzoldt.

At least 55 records · Page 3Linked to original sources
PubMed1979

[Development of an experimental animal model of aerogenic immunization with bacterial antigens (author's transl)].

Gnotobiotic piglets once aerogenically vaccinated with a avirulent strain, B10, of live Erysipelothrix rhusiopathiae (E.r.) showed no signs of illness when exposed to 100 LD50 of the highly virulent E.r.-strain FFA two weeks later. Mice vaccinated in the same manner were not fully protected. Various serological assays provided no indication for an actual protection mechanism against the challenge infection. On the other hand preliminary evaluation of the reactivity of lymphoid cells from various organs showed remarkable differences in the response to the vaccinating antigen and to various mitogens between vaccinated and non vaccinated piglets. Developmental phases of the model system and its advantages and disadvantages are presented.

Aerosols↗
PubMed1978

Passive allergisation of calves and lambs due to colostral antibodies.

Using an aqueous extract of liver fluke (Fasciola hepatica) as a naturally occurring antigen it was demonstrated that newborn calves were passively sensitized by oral intake of maternal anaphylactic antibodies. In 3 day-old, colostrum deprived calves and lambs the transfer of this anaphylactic activity was studied by Prausnitz-Küstner test and passive cutaneous anaphylaxis test. The technical conditions of the in vivo detection of anaphylactic antibodies are described. The relevance of passive anaphylaxis is discussed.

Animals↗
PubMed1976

Experiments with a model of aerosol immunization of mice and swine against Erysipelothrix insidiosa (E. i.).

Whole antigens as well as antigen fractions from E.i. were tested as aerosol vaccines. The aerosol produced by a jet nebulizer (Pari, Standard 088) with a capacity of 15 1 air per min and about 10g vaccine per hour was tested by size measuring of the aerosol particles and bacteriological evidence of E.i. in lung tissue. The main concern of the studies was to test the immunogenicity for laboratory animals which were exposed to the vaccines in a modified Henderson apparatus or in boxes of 30 1 respectively. Vaccination trials with conventional mice and piglets did not produce 100% protection against challenge infection by aerosol or subcutaneous route. Experiments with gnotobiotic piglets resulted in sufficient immunity against aerosol challenge with 100 LD50 when aerosols with living avirulent bacteria (strain B 10) were applied three times for one hour or once during three hours. A commercial vaccine from lyzed bacteria immunized only when aerosols were applied three times for three hours. For further immunological evaluation the sera of piglets were tested by means of an indirect fluorescent technique and by other serological methods.

Aerosols↗