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Biomedical subjects

K Ono

Publications and source records attributed to K Ono.

At least 1,009 records · Page 56Linked to original sources
PubMed1989

Long-term results of total hip arthroplasty for osteonecrosis of the femoral head. A comparison with osteoarthritis.

For reappraisal of total hip arthroplasty (THA) in the treatment of nontraumatic osteonecrosis (ON) of the femoral head, 29 hips in 23 patients with ON were surveyed in a comparison with 63 osteoarthritic (OA) hips treated in the same manner during the same period; the hips were selected according to the same criteria. The 29 ON hips were followed for an average of seven years. Comparing the ON and OA groups, the patient profiles were similar, except for weight and sex. For the ON group, hip scores according to the Merle D'Aubigne system (18 points for a normal hip) were improved from 9.8 preoperatively to 14.3 at the latest survey. However, 14 hips (48%) presented unsatisfactory clinical results, and roentgenologically all showed aseptic component loosening. Such a failure rate was higher than that for the OA group (33%); similarly, femoral component loosening occurred more frequently in the ON (28%) than in the OA group (5%). Based on the analysis of the patient profiles, Stage 3 ON at surgery, bilateral occurrence of the disease and bilateral THA could have caused such a failure in the ON group. In addition, most of the hips with component loosening showed histologic evidence of extensive bone necrosis within the resected femoral head. ON is a high-risk disease in terms of the long-term durability of THA, the failure of which seems to be mainly caused by extensive necrosis.

Adult↗
PubMed1989

[Role of bile glycoprotein in intrahepatic calculus formation].

To clarify the etiology of intrahepatic calculus formation, we examined the components of hepatic bile, particularly glycoprotein, in 20 controls and 10 patients with intrahepatic calculi. The results obtained were summarized as follows: 1) The yield of delipidized bile powder was approximately 2.5 times higher in intrahepatic calculosis than in control. Quantitative analysis revealed that protein and neutral sugar were significantly increased in intrahepatic calculosis as compared with those in control. In SDS-PAGE of the powder, both intrahepatic calculosis and control groups demonstrated almost similar patterns except for difference in stainability between groups. This indicates that glycoprotein, that had been already present in control bile, was markedly increased in hepatic bile in intrahepatic calculosis. SDS-PAGE also demonstrated a thick and deep-stained band near the starting point only in intrahepatic calculosis. 2) Chromatography obtained from gel filtration on Sepharose CL-6B revealed 2 large peaks, one in void volume (macromolecular) and the other in total volume (micromolecular) region. In chemical analysis, macromolecular fraction in intrahepatic calculosis was rich in galactosamine, fucose and sialic acid, and characterized by prominent serine and threonine, which indicated that it was composed mainly of mucin type sialoglycoprotein. The micromolecular fraction in both groups were rich in aspartic acid and mannose, which indicated that they were composed mainly of serum type glycoprotein.

Adult↗
PubMed1989

[A case of aortitis syndrome with anastomoses from left coronary artery to bronchial artery].

We reported a case of aortitis syndrome with about 10-year course from onset. The patient was a 44-year old female suffering from a cardiac failure. The angiogram revealed presence of aortic regurgitation, The angiogram revealed presence of aortic regurgitation, pulmonary vascular lesions, anastomosis from left coronary artery to bronchial artery and hypervascularity of bronchial artery. We considered the anastomosis from left coronary artery to bronchial artery as a collateral blood flow for the ischemic lesions of the lung. We abandoned aortic valve replacement because of the severe pulmonary vascular lesions and of the pulmonary dysfunction.

Adult↗
PubMed1989

Elevated activity of myeloid growth factor in bone marrow adjacent to joints affected by rheumatoid arthritis.

Myeloid growth activity of rheumatoid arthritis (RA) and normal serum was measured with a newly developed simple method. Abnormally high titers of this activity were found in the bone marrow blood serum adjacent to joints with RA where abnormal myelopoiesis had been found. Maximal activity for myeloid growth was found at a molecular weight of 70 kDa in gel filtration of serum, different from the size of any known factors involved with myelopoiesis.

Adult↗
PubMed1989

[Urinary cytodiagnostic abnormality in a patient with malignant lymphoma].

A 69-year-old man was admitted due to chronic subdural hematoma. He had lymphadenopathies in the neck. The diagnosis of non-Hodgkin lymphoma (diffuse, large, B cell type) was made by a cervical lymph node biopsy. The renal failure by obstruction of the urinary tract developed gradually, and the tumor cells were found by urinary cytodiagnosis. He was treated immediately with chemotherapy. After the treatment, the urine volume increased and the general conditions improved. It was indicated from this case that the urinary cytodiagnosis is useful for the detection of urinary tract infiltration by lymphoma cell. The urinary cytodiagnostic abnormalities in patients with non-Hodgkin lymphomas were discussed from the literature, including this case.

Aged↗
PubMed1989

[Repair of rabbit articular surfaces with allografts of chondrocytes embedded in collagen gels].

In an attempt to restore articular cartilage, allogeneic articular chondrocytes embedded in collagen gels were transplanted onto full-thickness defects in rabbit articular cartilage. Within 24 weeks after the transplantation, the defects were filled with hyaline cartilage, specifically synthesizing type II collagen. These chondrocytes were autoradiographically proven to be originated from the originally transplanted chondrocytes. As histologically assessed, success rate was about 80%, a marked improvement over the results (40% success rate) in previous studies reporting chondrocyte transplantation without collagen gels. On the other hand, the defects without chondrocyte transplantation healed with fibrocartilaginous tissue more than 24 weeks after treatment. Immunological enhancement induced by transplanted allogeneic chondrocytes or collagen was not significant for eight weeks after treatment, so far as shown by both direct and indirect blastformation reactions. Thus, allogeneic transplantation of isolated chondrocytes embedded in collagen gels appears to be one of the most promising methods for the restoration of articular cartilage.

Animals↗
PubMed1989

[Operative results in cancer of the pancreas, especially complicated with large vascular involvement].

Surgical treatment has been performed in 25 cases of cancer of the pancreas. Localized large vascular involvements of portal vein, celiac axis, common hepatic artery and/or superior mesenteric artery by the pancreatic cancer were observed in 11 cases, which necessitated total or subtotal pancreatectomy associated with segmental resection of the vessels with vascular reconstruction in 7 cases but in remaining 4 cases resectional procedure was not performed. Curative pancreaticoduodenectomy was carried out in 5 cases without large vascular involvement and non-curative pancreatectomy in one case. Exploratory laparotomy or only anastomosis for the bile duct or the intestine was done in 8 cases. The operative resectability rate was 52% and the curative one was 44%. The regional pancreatectomy seemed to contribute to improvement of resectability. However, one year survival rate was only 42% (2 year, 0%) in the curatively resected cases with large vascular involvement, while 5 year survival rate was 67% in those without vascular involvement. The quality of life after extended total pancreatectomy was distressful. On the other hand, two extended totally pancreatectomized cases with successful autotransplantation of distal pancreas are living at one year and 3 months after operation respectively, and enjoying the common social life at present.

Aged↗
PubMed1989

Combined effects of radiation and IUdR on experimental liver tumors and hepatocytes.

IUdR, a thymidine analogue, increased the radiosensitivity of SCC VII tumor cells. A decrease in Dq value of cell survival curve was more prominent compared with that in D0 value. However, in experimental liver tumors made by transplanting SCC VII tumors, the decrease in D0 value was apparent. Proportion of IUdR incorporated tumor cells in the liver increased with an increase in number of IUdR injections, and reached 52% after five administrations of the drug at an interval of twelve hours. However, few hepatocytes incorporated IUdR. The response of hepatocytes to radiation was evaluated by micronucleus appearance frequency. To accelerate the appearance of micronucleus in hepatocytes, partial hepatectomy was performed immediately after irradiations. No differences in micronucleus frequency were observed between the treated and untreated with IUdR.

Animals↗
PubMed1989

Periosteal bone formation elicited by partially purified bone morphogenetic protein.

A small amount of partially purified, water-soluble murine osteosarcoma-derived bone morphogenetic protein (BMP) was implanted into the dorsal muscles of mice in combination with calf skin gelatin or collagen as carriers. Changes in the ribs adjacent to the implants were then chronologically observed. On implantation of BMP with gelatin, the gelatin was rapidly absorbed and no ectopic bone formation was observed, but periosteal cellular proliferation with subsequent formation of periosteal cartilage and bone was seen in ribs adjacent to the implant. Implantation of the same amount of BMP fraction or gelatin alone as controls did not result in either any ectopic bone formation in situ or any periosteal bone formation in adjacent ribs. Implantation of BMP with collagen resulted consistently in both ectopic bone formation in situ and periosteal bone formation in adjacent ribs. These results suggest that BMP is diffusible in vivo and is capable of eliciting a response from periosteum to stimulate periosteal bone formation.

Animals↗
PubMed1989

The effect of vitamin C supplementation and withdrawal on the mortality and morbidity of regular hemodialysis patients.

The present study was undertaken to evaluate the effects of vitamin C supplementation (VC-S) on the morbidity and mortality of 61 clinically stable outpatients maintained on regular hemodialysis (HD). All patients were given vitamin C (500 mg daily) for 2 years and observed for a further 2 years on no treatment. VC-S significantly increased the plasma levels of ascorbic acid up to 7.8 mg/dl (mean 3.3 +/- 0.4 s.e.m.) which fell after withdrawal to the normal range (mean 1.2 +/- 0.2 mg/dl). Hyperoxalemia was aggravated by VC-S (mean 61.5 +/- 3.3 mumol/l, range 33.3 to 165.5) while plasma oxalate levels in the unsupplemented period decreased to 36.3 +/- 3.3 mumol/l (p less than 0.01). There were no differences in creatinine, hematocrit, blood transfusion requirement, morbidity (including hospitalization) or mortality between the two periods of time in the same patients. In conclusion, we could not find any beneficial effects on morbidity or mortality as a result of using VC-S in regular HD patients. However, secondary hyperoxalemia was aggravated. As a result of these observations it appears that VC-S is harmful and unnecessary in these patients provided they are on an adequate diet.

Ascorbic Acid↗
PubMed1988

Premotor cortical kindling interferes with subsequent hippocampal kindling.

Interaction between the neocortical motor system and the limbic system was investigated in terms of the transfer of kindling. Premotor cortical kindling induced limbic seizures accompanied by dissociated hippocampal afterdischarges (AD) which outlasted the premotor AD. Subsequent kindling of the hippocampus was markedly retarded despite prolonged hippocampal AD. These results suggest that premotor cortical kindling induces a neuronal mechanism which prevents limbic access to the motor system.

Animals↗
PubMed1988

Prognostic significance of mast cells in soft tissue sarcoma.

We counted the number of toluidine blue positive mast cells within and around the tumor in 44 patients with soft tissue sarcomas (STS). Irrespective of their histologic types, these cases were broadly divided into the following two groups: (1) low count (less than 20 mast cells/10 high-power fields [HPF]) and (2) high count (greater than or equal to 20 mast cells/10 HPF). The patients with a high mast cell count showed a significantly better 5-year survival rate than those with a low count (85.9% versus 30.5%; P less than 0.01). Five patients with distant metastases at first admission all belonged to the low count group. These results suggest that the number of mast cells within and around the tumor is a useful prognostic factor for STS.

Adolescent↗
PubMed1988

Differential inhibition of various deoxyribonucleic acid polymerases by Evans blue and aurintricarboxylic acid.

The inhibitory effects of two anionic compounds, Evans blue and aurintricarboxylic acid (ATA), on various kinds of polynucleotide-synthesizing enzymes were examined. Under the assay conditions, optimized for each enzyme species, both these compounds strongly inhibited the activities of the purified human DNA polymerases alpha, beta, gamma, and DNA primase as well as those of DNA polymerase I and RNA polymerase from Escherichia coli and Rauscher leukemia virus reverse transcriptase. ATA was particularly effective in inhibiting retroviral reverse transcriptase and cellular DNA polymerase alpha. Evans blue, which is a structural analogue of suramin, exerted its inhibitory action largely by competing with the template.primer for the same binding site of the enzyme. On the other hand, ATA inhibited most, if not all, of these enzyme activities noncompetitively with respect to either the template.primers or nucleoside 5'-triphosphate substrates. The inhibition constants for ATA were, in general, smaller than those for Evans blue.

Animals↗
PubMed1988

Isolation and characterization of sulfated glycoprotein from human pancreatic juice.

Sulfated glycoprotein was isolated by precipitation from dialyzed human pancreatic juice and purified by ion-exchange chromatography followed by repeated gel chromatography. The sulfated glycoprotein was obtained as a sulfated glycoprotein-lipid complex by Sepharose CL-2B chromatography. Lipids aggregating with the sulfated glycoprotein were glycolipids such as ceramide trihexoside, and simple lipids such as cholesterol and cholesterol ester. This glycoprotein was resistant to digestion with mucopolysaccharidases or alpha-amylase, and consisted of 60% (w/w) protein and 40% sugars. The polypeptide core was characterized by a high content of serine, threonine, aspartic acid and glycine, but lacked cysteine. Its sugar components were N-acetylglucosamine, N-acetylgalactosamine, galactose, fucose and sialic acid. Absorption at 1240 cm-1 and 820 cm-1 by infrared spectroscopy indicated the presence of a sulfate ester group. All the carbohydrate chains of this sulfated glycoprotein, which are polydisperse and heterogeneous, were O-glycosidically linked through N-acetylgalactosamine to a protein core.

Amino Acids↗
PubMed1988

Multivariate analysis for clinical prognostic factors in 163 patients with soft tissue sarcoma.

Clinical and histologic findings in 163 patients with localized soft tissue sarcoma (STS) in the extremities and trunk were reviewed. There were 91 male patients and 72 female patients ranging in age from 2 to 84 years (median, 46 years). The histologic status of the tumors was as follows: low grade, 29 cases; intermediate grade, 52 cases; and high grade, 82 cases. The primary tumors were treated by intralesional excision (two cases), marginal (88), wide local (52), or amputation (21). Subsequent adjuvant therapy was given to 61 patients; 17 had radiotherapy (RT), 27 had chemotherapy (CH), and 17 had combined RT and CH. The overall survival (P less than 0.1) and disease-free survival (P less than 0.001) were better in the group that received multimodal treatment (radical surgery and adjuvant chemotherapy with or without radiotherapy) than in the group treated only by surgery. This suggests the favorable role of adjuvant therapy. The univariate and the Cox multivariate analysis for prognosis revealed that sex, tumor-related symptoms, tumor size, tumor depth, and histologic grade were the significant factors. Among the treatment schemes, adjuvant chemotherapy was the only one that affected survival, especially for the intermediate-grade tumors. Initial surgical treatment (marginal versus wide local excision) significantly contributed to the local control of the primary tumors.

Actuarial Analysis↗
PubMed1988

Direct adrenergic inputs to sacral autonomic neurons: using a double-immunostaining method at the light and electron microscopic levels.

Relationships between adrenergic fibers and cholinergic neurons were examined in the rat sacral intermediolateral nucleus using a double-immunostaining method at light and electron microscopic levels. Adrenergic fibers were immunohistochemically stained brown by peroxidase reaction, and cholinergic neurons in the same sections were stained bluish green by beta-galactosidase reaction. In the light microscope, many cholinergic neurons were seen in the intermediolateral nucleus and some of them were surrounded by adrenergic fibers. In the electron microscope, adrenergic fibers made synapses with the cholinergic neurons in the intermediolateral nucleus. These results suggest that adrenergic fibers directly influence the activity of the sacral parasympathetic preganglionic neurons.

Animals↗
PubMed1988

Differential inhibition of various mammalian DNA polymerase activities by ammonium 21-tungsto-9-antimoniate (HPA23).

The 21-tungsto-9-antimoniate ammonium salt (HPA23), known as an antiviral agent, has been shown to be a potent inhibitor of both human and murine DNA polymerase alpha and murine DNA polymerase gamma. HPA23 inhibited the activity of DNA polymerase alpha in noncompetitive fashion with respect to either deoxynucleotide substrate or nucleic acid template.primer. The Ki of murine DNA polymerase alpha for HPA23 was determined to be 24 nM. The activity of mouse DNA polymerase gamma also was strongly inhibited by HPA23 (Ki, 20 nM), and the mode of inhibition was competitive with respect to the template.primer, (rA)n.(dT)12-18, and noncompetitive to substrate, dTTP. DNA polymerase beta and terminal deoxynucleotidyltransferase, however, were relatively resistant to inhibition by HPA23. The observed inhibitions by HPA23 seem to be closely related to the polyanionic property of this drug.

Animals↗