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Biomedical subjects

K Ohno

Publications and source records attributed to K Ohno.

At least 685 records · Page 38Linked to original sources
PubMed1985

[Distribution of cefotiam dihydrochloride to the bloodless lung].

The concentration of cefotiam (CTM) in the serum and the bloodless lung with time is discussed. Five patients who were undergone pneumonectomy or lobectomy, were given 1 g of CTM intravenously during the operation. Blood samples and lung samples were collected 15, 30, 60, 90, 120, and 300 minutes after the administration. The CTM levels in the blood and the lung samples were measured by the agar well bioassay. The CTM levels in the lung samples were modified as the CTM levels in the bloodless lung by calculating the contained blood volume, as lung samples were proven to have a mean value of 34.2% of blood in weight. The serum CTM level was 73.3 micrograms/ml at 15 minutes after injection and then decreased gradually. The CTM level in bloodless lung was elevated during the first 60 minutes, became equal to that of the blood during next 30 minutes and then exceeded the serum levels thereafter. These results indicate that the distribution of CTM to the bloodless lung was excellent and a satisfactory preventative effect against postsurgical infection could be expected.

Aged↗
PubMed1985

[Clinical studies on TMS-19-Q.O tablets, the preparation of a new macrolide antibiotic, in the field of oral surgery].

Multicenter clinical trial or TMS-19-Q.O tablet was performed to evaluate the usefulness in oral surgery. The results obtained were as follows: Clinical efficacy was assessed by clinical points. The patients entered into the trial were 77 cases with periodontitis, 23 with pericoronitis, 92 with osteitis of jaw and 18 with other infections, and the each effective rates were 80.5, 60.9, 83.7 and 72.2%, respectively. Overall effective rate was 79.0%. Isolation frequency of organisms were 33.8% in periodontitis, 34.8% in osteitis of jaw and 17.4% in pericoronitis. Bacteria isolated were aerobic organisms (63.1%) and anaerobic organisms (36.9%). TMS-19-Q.O tablet was well tolerated, and no adverse reaction was observed.

Adolescent↗
PubMed1985

[Topographic mapping of regional cerebral blood flow by xenon-enhanced CT--introducing a simplified method as a routine examination].

Measurement of regional cerebral blood flow (rCBF) has become a routine examinations in the field of clinical neurology and neurosurgery. Therefore, various methods for measuring rCBF have been developed. Among them, xenon-enhanced CT method has many advantages compared with others. An obtainable topographic flow map, high anatomical spatial resolution, and readily available instrument, namely CT scanner, are regarded as the advantages. On the one hand, anesthetic effects of xenon gas on the patients, taking a great expense for a single examination, and a significant dose of radiation delivered to the patients are thought to be the disadvantages. Due to the disadvantages, xenon-enhanced CT is regarded as an unpractical examination. In the paper, we introduce a method with a brief xenon inhalation, named a simplified method, with which the disadvantages can be avoided. Conventional xenon-enhanced CT requires 20 to 25 minute inhalation of xenon gas in order to achieve a saturation of xenon in the cerebral tissue for a calculation of the partition coefficients (L). In the simplified method, instead of using the calculated L, L was given as 1.0 in all the cerebral regions and xenon gas was terminated at 4 minute inhalation. Four minute inhalation of xenon gas did not make any significant anesthetic effects on the patients nor the changes of physiological parameters, such as, PaCO2 and blood pressure.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Volume Determination↗
PubMed1984

Regional cerebral blood flow and stroke index after left carotid artery ligation in the conscious gerbil.

We measured the regional cerebral blood flow in both hemispheres of Mongolian gerbils subjected to permanent left carotid artery ligation, using [3H]nicotine as the tracer. At 1, 3 and 6 h post-ligation, neurological signs were recorded and a stroke index score was tallied for each animal. In conscious control gerbils, mean cerebral blood flow on the left side was 1.10 +/- 0.08 (S.E.M.) ml X g-1 X min-1 at the cerebral cortex, 0.58 +/- 0.02 at the hippocampus and 0.69 +/- 0.04 at the diencephalon. Animals with a stroke index score exceeding 10 were considered symptomatic. We noted a close relationship between regional cerebral blood flow and the stroke index score. In symptomatic animals, regional cerebral blood flow in the ischemic hemisphere at 1, 3 and 6 h post-ligation was less than 0.21 ml X g-1 X min-1 at the cortex and diencephalon, and less than 0.09 ml X g-1 X min-1 at the hippocampus. We suggest that unilaterally ligated gerbils manifesting a stroke index score greater than 10 represent a good experimental model for the study of ischemia.

Animals↗
PubMed1984

Computed tomography in congenital ocular motor apraxia.

Unusual computed tomographic findings were observed in four patients with congenital ocular motor apraxia (COMA). These (CT) findings were characteristic in the posterior cranial fossa, with a dilated or deformed shape and size of the fourth ventricle, particularly its upper portion. One case revealed partial agenesis of the cerebellar vermis. It was suggested that the four patients subjected to CT showed abnormal topography of the cerebellar vermis or the brain stem. We postulate that these CT findings in COMA may have an important role in its pathogenesis.

Apraxias↗
PubMed1984

Genetic analysis of control of proliferation in fibroblastic cells in culture. I. Isolation and characterization of mutants temperature-sensitive for proliferation or survival of untransformed diploid rat cell line 3Y1.

Mutants temperature sensitive for proliferation or survival were isolated from an untransformed diploid clone of fibroblastic rat cells (3Y1), according to an isolation protocol that selected for mutants defective at 38.5 degrees C (selection temperature) in undergoing the transition from quiescent to proliferating state while maintaining viability at 38.5 degrees C. Of the 108 temperature-sensitive clones isolated, 32 were examined for survival in sparse cultures at 39.8 degrees C (nonpermissive temperature) and classified into four classes. Results of temperature shift-up experiments suggest that functions defective in 11 of the 32 mutants are necessary not only for changing from the quiescent to proliferating state but also for maintenance of the proliferating state. Of the 32 mutants, 17 were assigned to eight complementation groups. Results of the physiological characterization of the representative mutants of each of the eight complementation groups are presented.

Animals↗
PubMed1984

Genetic analysis of control of proliferation in fibroblastic cells in culture. II. Alteration in proliferative and survival phenotypes in a set of temperature-sensitive mutants of rat 3Y1 cells after infection or transformation with simian virus 40.

Mutants of rat 3Y1 fibroblasts, temperature sensitive for proliferation or survival and which represent each of eight complementation groups, were examined to determine whether cells made quiescent at confluence at 33.8 degrees C (permissive temperature) can be stimulated to enter S phase at 39.8 degrees C (nonpermissive temperature) by 20% serum or by infection with simian virus 40 (SV40). Three mutants with a short survival at 39.8 degrees C did not enter S phase at 39.8 degrees C under either condition. The remaining five entered S at 39.8 degrees C by infection with SV40. However, only one of these five entered S in response to high serum. After transformation with SV40, three mutants accumulating at 39.8 degrees C with a predominantly 2n (G1) DNA content did not proliferate, there was a rapid and extensive cell death, and the cells had a DNA content similar to that seen in randomly proliferating populations. The other two mutants, accumulating at 39.8 degrees C with a predominantly 2n or 2n X 4n DNA content, proliferated at this temperature after transformation with SV40. These results clearly indicate that SV40 interacts closely with cellular ts lesions related to control of proliferation and cell survival.

Animals↗