Biomedical subjects
Publications and source records attributed to K Miller.
The following case report describes the evaluation, assessment, and treatment of a facial infection due to Salmonella typhi, caused by bites from the family's pet dog. The causative agent and the mode of transmission are unique in that there are no reported cases of this nature in the literature at this time.
Explore the source record for details and available documents.
The localization of a platelet antigen or antigens kidneys from 106 patients with renal disease was evaluated with immunofluorescent microscopy by using a rabbit antibody to human platelets. The antiplatelet IgG fixed to the surface membrane of platelets did not react with erythrocytes, leukocytes, plasma, normal kidney, or a variety of normal tissue targets. Significant glomerular and vascular deposition of platelet antigen (or antigens) was observed along the endothelium or as vascular plugs in kidney tissue from patients with hemolytic uremic syndrome, membranoproliferative glomerulonephritis (types I and II), diabetic nephropathy, hypertensive renal disease, scleroderma, and other diseases. Dual-label immunofluorescent studies revealed that platelet antigen (or antigens) and fibrinogen/fibrin-related antigen (FRA) were usually, though not always, present in similar loci. Platelet antigens were not observed at sites of intense FRA deposition: in the mesangium in anaphylactoid purpura and in glomerular crescents in Goodpasture's syndrome. Platelet antigen was detected in the peritubular capillaries of most patients with diabetic nephropathy.
Younger (mean age = 20) and older (mean age = 64) adults were asked to recall the dates and make recency judgments for historical events that occurred in each of three time periods between 1862 and 1977. Overall there were no age differences in either the number of correct dates or the number of correct recency judgments. However, younger adults tended to perform better than older adults on events that occurred most recently, and performed significantly better on events that occurred during the respective times of their youth. In addition, somewhat different patterns of performance on the two dependent measures suggested that detailed information about events is lost with time, but that more general information may not decline with time.
Between October 1977 and 1978, 93 CutterCast casts were applied at the Veterans Administration Hospital in Atlanta, Georgia. The composition and application of this new casting system are described, and the performance of the 93 casts is evaluated in relation to ease of application, roentgenographic clarity, skin condition, patient comfort, and ease of removal. The advantages and disadvantages are discussed.
Human cord blood mononuclear cells (MNC) were stimulated with antibiotic killed Staphylococcus aureus Cowan I bacteria (S. aureus) or pokeweed mitogen (PWM) and cultured for 6 days. Immunoglobulin synthesis and secretion was measured with the indirect protein A plaque-forming cell (PFC) assay. All 14 cord blood specimens stimulated with S. aureus produced PFC comparable to that measured in adult peripheral blood cultures. PWM induced only minimal responses in cord MNC whereas good responses were demonstrated in adult cell cultures. The majority of the cord blood immunoglobulin synthesizing cells produced IgM, however, significant production of IgA was also seen. These observations were confirmed by detection of intracytoplasmic immunoglobulin in plasma cells with the use of fluorescent anti-immunoglobulin antibodies.
The effects of inhalation of fibre-glass fibres over a prolonged period on certain membrane-related characteristics of rat alveolar macrophages have been investigated. Macrophage populations varied in size from 10-40 micrometers and showed an enhanced ability to spread across a glass substrate. An increase in the percentage of cells forming EA rosettes as well as an increased avidity of Fc receptors when compared with control macrophages was observed. When macrophages from fibreglass-dusted rats were cultured in vitro with autochthonous lymphocytes, a prolonged physical interaction occurred. These observations closely parallel these made in earlier studies on animals exposed to crocidolite asbestos.
The effect of prolonged inhalation of crocidolite asbestos on the physical interactions between alveolar macrophages and the T-lymphocyte population was investigated. When macrophages from dusted rats (dusted macrophages) were cultured with lymphocytes, a prolonged physical interaction occurred followed by lymphocyte proliferation. It was demonstrated that dusted macrophages could bind lymphocytes nonspecifically due to the presence of aldehyde-like groups on the membrane. Dusted macrophages could also, as a consequence of antigen-like molecules appearing in the membrane, give rise to specifically sensitized lymphocytes.
An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
In conditions of declining water PO2, Xenopus obtains the majority of resting oxygen needs from lung breathing at 15 degrees and 25 degrees C. The critical oxygen tension was 120 +/- 9 mm Hg at 15 degrees C, and 90 +/- 10 mm Hg at 25 degrees C. During 30 min stimulation of activity to complete exhaustion at 15 degrees C, frogs exhibited an aerobic capacity of 1.7 microliter O2.g-1.h-1 and accumulated 2.22 mg lactate . g-1. Following activity these animals exhibited an oxygen debt of 49.2 microliter O2.g-1. At 25 degrees C, Xenopus had an aerobic capacity of 16.1 microliter O2.g-1 and accumulated 1.94 mg lactate . g-1. Following activity, 25 degrees C frogs exhibited an oxygen debt of 261.9 microliter O2.g-1. Thus at 15 degrees C, Xenopus acquires 85% of the ATP used during a bout of sustained exhaustive activity from anaerobic sources, and 98% at 25 degrees C. Recovery from exhaustive activity was complete after 5 h at 25 degrees C and 9 h at 15 degrees C. Comparison of these data with those of other amphibians stimulated to sustained activity suggests that aquatic amphibians can tolerate a larger lactate load than can terrestrial forms.
Explore the source record for details and available documents.
Previous studies have shown conflicting results with respect to the relationship between C6 deficiency and the HLA system. The present investigation of two kinships, in which C6 deficiency was associated with the expression of an amorphic (or silent) C6 allele, has provided further evidence for the genetic independence of a structural C6 locus and the HLA system.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.