Biomedical subjects

K Matsui

Publications and source records attributed to K Matsui.

905 records · Page 51Linked to original sources

PubMedDate not supplied

Cytokinetic and cytotoxic responses of HeLa cells to the combination of hyperthermia and radiation.

Colony formation and cell cycle changes of HeLa cells were studied after the combination of hyperthermia and 60Co irradiation. Cells were heated by immersion in a water bath. The cell cycle progression was monitored using flow cytometry. The dose survival curve of asynchronous growing cells, with immediate postirradiation thermal treatment at 43 degrees C for 60 min, showed significant enhancement of radiosensitivity. When the sequence of heat and irradiation was varied, the greatest decrease in cell survival was obtained when irradiation was given immediately before or after thermal treatment. Flow cytometry analysis showed that irradiation of exponentially growing cells with 5 Gy induced a G2 block, and that heat treatment immediately after 5 Gy exposure caused a progression delay of the cells from G2 + M to G1 phase. Furthermore, the cellular DNA histogram at 48 hours after 5 Gy exposure, immediately before heating, had more cells in G2 + M phase as compared with cells exposed to 5 Gy alone or thermal treatment at 5 hours before or after 5 Gy exposure. These results suggest that hyperthermia immediately after irradiation inhibits some of the cells accumulated in G2 + M phase from traversing into G1 phase. This inhibition may be related to the synergistic cell-killing by hyperthermia and radiation.

Cell Cycle↗

PubMedDate not supplied

Dynamic lymphoscintigraphy with Tc-99m human serum albumin.

Dynamic studies of the lymphatic systems of 26 cases, of which 4 were healthy volunteers, were performed using an intradermal injection of Tc-99m human serum albumin (Tc-99m HSA). With the assistance of a computer, sequential images and time-activity curves of lymphatic vessels and/or lymph nodes were obtained for a period of 30 min after the injection from the cases who took no physical exercise. The image of the axillary or inguinal lymph nodes was identified 2-4 min after injection in cases with a normal lymphatic flow. The delayed appearance of radionuclide, the collateral pathways and the obstruction of the lymphatic system were clearly observed in patients with a flow disorder. The clearance rate at the injection site of Tc-99m HSA was approximately twice as fast as that of Tc-99m rhenium sulfide colloid. The high pressure in the dense connective tissue caused by intradermal injection and the high specific radioactivity of Tc-99m HSA were thought to be important factors for obtaining excellent dynamic lymphoscintigraphy.

Adult↗

PubMedDate not supplied

Sex differences in clinicopathological features of peripheral T1 adenocarcinoma of the lung.

Clinicopathological features of surgically-treated peripheral T1 adenocarcinoma of the lung were compared between 30 females and 26 males, and the following sex differences were observed; 1) Females were younger than males, 2) There was a higher percentage of smokers among males, 3) The acinar histologic subtype was less frequently found in females, 4) Well differentiated tumors were more frequently found in females, 5) K-ras gene mutations were observed only in males, 6) Prognosis was slightly better in females. As to other factors such as N-factor, tumor ploidy or central fibrosis, there was no statistical difference between the two groups. Although we were not able to explain the causes for the above sex differences, it was speculated that smoking was mainly responsible for them.

Adenocarcinoma↗

PubMedDate not supplied

Asymptomatic hemobilia with idiopathic thrombocytopenic purpura.

A severe but asymptomatic anemia developed in a patient with idiopathic thrombocytopenic purpura. Urgent upper gastrointestinal endoscopy disclosed massive bleeding from the papilla of Vater and we inferred that the anemia was caused by hemobilia. The bleeding point could not be detected, but the bleeding was stopped with conservative therapy. Although several cases of hemobilia have been reported in patients with bleeding tendency, this is the first case to be reported in a patient with idiopathic thrombocytopenic purpura.

Adult↗

PubMedDate not supplied

MR imaging of perinatal brain damage: comparison of clinical outcome with initial and follow-up MR findings.

BACKGROUND AND PURPOSE: The purpose of our study was to determine whether MR studies in the neonatal period are predictive of the neuroradiologic sequelae and clinical outcome in premature and term infants with perinatal brain injury. METHODS: Thirty subjects (15 premature and 15 term infants) with abnormalities revealed by initial MR studies were reexamined approximately 1 year after birth with both MR imaging and a neurologic assessment. All initial MR studies were performed between 35 and 45 weeks corrected age in premature infants and within 28 days of life in term infants. The initial MR studies were evaluated for deep gray matter involvement, hemispheric parenchymal change, intracranial hemorrhage, and periventricular signal and/or morphologic changes. These MR findings were compared with the follow-up MR findings and with the neurologic outcome. RESULTS: The development of cerebral palsy in premature infants was related to the following initial MR findings: subependymal hemorrhage associated with parenchymal destruction, periventricular signal alteration with irregularity of the ventricular wall, and widespread cerebral infarction. These MR findings were predictive of the subtypes of cerebral palsy. In term asphyxiated infants, T2 signal alterations of the deep gray matter rather than T1 shortening and diffuse involvement of the hemispheres were predictive of an unfavorable outcome. Both in term and premature infants, focal hemispheric parenchymal lesions alone (including infarction and intracerebral, subdural, intraventricular, and subarachnoid hemorrhage) did not produce poor outcomes. CONCLUSION: MR studies performed at or near term in either premature or term infants with perinatal brain damage are effective in predicting both late neuroradiologic and clinical outcome.

Asphyxia Neonatorum↗