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Biomedical subjects

K Masuda

Publications and source records attributed to K Masuda.

At least 613 records · Page 34Linked to original sources
PubMed1987

[Clinical study on effects of cefmetazole on severe infections accompanied by hematologic diseases].

Severe infections accompanied by hematopathy under granulocytopenic conditions were treated with cefmetazole (CMZ). Subject diseases mainly consisted of acute leukemia, agranulocytosis and aplastic anemia; combined infections were septicemia, pneumonia, fever of an undetermined origin, etc. As for causative organisms found in cases that could be examined, Gram-negative bacilli such as Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa and Enterobacter cloacae were isolated, as was Staphylococcus aureus. In general, 4 g of CMZ divided into 2 administrations was given per day through intravenous injection or intravenous drip infusion. On the basis of the judgement criteria for effectiveness established by Takaku et al., the efficacy rate in this study was found to be 68%, including 2 cases that showed excellent responses to treatment of infections caused by S. aureus. Cases that showed pyretolysis within 4 days had over 1,000/microliter of neutrophils, while cases with less than 1,000/microliter showed no pyretolysis. No hepatorenal dysfunctions related to the treatment with CMZ were seen as side effects except increases of transaminase in 1 case. These results indicate that CMZ is a useful drug for the treatment of infections accompanied by hematopathy under granulocytopenic condition.

Adolescent↗
PubMed1987

In vivo effects of prostaglandins on human retinoblastoma cells in nude mice.

Prostaglandin D2 (PGD2) and 64E, a derivative of PGs, were tested for their capacity to suppress the growth of retinoblastoma in the nude mouse. Ten million cells of an established cell line of human retinoblastoma, Y-79 cells, were transferred subcutaneously into the nude mouse, and after the transferred cells became a tumor with a diameter larger than 7.5 mm, either PGD2 (1, 2, or 4 mg/kg/day) or 64E (4 mg/kg/day) dissolved in Hanks' solution was daily injected subcutaneously near the tumor for 14 days. The estimated tumor weight as defined by the formula of (length) X (width)2/2 was evaluated at various time intervals after the treatment. Although a low dose of PGD2, 1 mg/kg/day, had no effect, higher doses of PGD2 had a clear effect in suppressing the growth of retinoblastoma in the nude mouse. Tumors in 64E-treated animals were also markedly suppressed in their growth. Histological examination revealed that tumors treated with these drugs had a much larger area of necrosis with fewer tumor cells than the tumors in control animals.

Animals↗
PubMed1987

[The tolerance dose of the rectum].

The incidence of rectal bleeding (Grade II or III) after radiotherapy for carcinoma of uterine cervix were studied. All cases were treated by external irradiation (ERT) and intracavitary irradiation (ICR). The dosage of rectal bleeding at 5% level were as followed; 40 Gy by ERT and 27 Gy at point A by ICR in National Sapporo Hospital, 36-40 Gy by ERT and 24 Gy at point A by ICR in Aichi Cancer Center, 40 Gy by ERT and 27 Gy at point A by ICR in Kyushu Cancer Center.

Dose-Response Relationship, Radiation↗
PubMed1987

Effect of neonatal thymectomy on experimental autoimmune hepatitis in mice.

Using an autoimmune hepatitis model of A/J mice which was prepared with immunization by syngeneic crude liver proteins, various influences of neonatal thymectomy were studied by observations of histological liver changes, autoantibody to liver-specific membrane lipoprotein (LSP), delayed-type hypersensitivity (DTH) to LSP, and purified protein derivative (PPD), and suppressor activity to LSP. The liver changes in the thymectomized mice were more intense than those in the non-thymectomized controls. Production of the anti-LSP autoantibodies and positive DTH to syngeneic LSP could be recognized in both groups of the thymectomized mice and the non-thymectomized controls, but the levels of those were higher in the former. In the level of DTH to PPD the thymectomized mice were lower than the non-thymectomized controls. Adoptive transfer experiments showed that suppressor activity to LSP was reduced in the spleen cells of neonatally thymectomized mice. This experiment suggests that neonatal thymectomy is apt to abolish tolerance to LSP on account of depressed suppressor activity to autoantigen, and accordingly liver damage is increased.

Animals↗
PubMed1987

[Rib fracture after postoperative tangential irradiation in breast cancer].

Incidence of rib fracture was analyzed for 118 postoperated breast cancer patients who were tangentially irradiated at the Department of Radiology, Kyushu University, from 1961 to 1976 and followed more than 5 years. Rib fracture was observed in 5% and 50% of cases treated with total radiation doses of 32.5 Gy and 52.5 Gy respectively.

Actuarial Analysis↗
PubMed1987

[Skin damage after postoperative tangential irradiation in breast cancer].

Incidence of telangiectasia and ulcer among 118 postoperated breast cancer patients, who were tangentially irradiated at Department of Radiology, Kyushu University from 1961 to 1980, followed more than 5 years were evaluated. Telangiectasia occurred in 5% at 20-35 Gy, in 50% at 50-55 Gy. Ulcer occurred in 5% at 50-55 Gy, in 50% at 70-75 Gy. Skin tolerance dose in Rubin's Table (1972) is correct.

Actuarial Analysis↗
PubMed1987

[Pulmonary fibrosis after postoperative tangential irradiation in breast cancer].

Incidence rate of pulmonary fibrosis with elevation of diaphragma was calculated for 118 postoperated breast cancer patients who were tangentially irradiated at the Department of Radiology Kyushu University from 1961 to 1980 and followed more than 5 years. 5%, 50% occurred at 40-45 Gy, 60-65 Gy, Rubin's Table (1972) is possible correct.

Actuarial Analysis↗
PubMed1987

[Thyroid dysfunction following external irradiation of the neck].

Thyroid function after radiation therapy to the neck was studied in 120 patients, 110 of whom were suffered from malignant lymphomas. No patients had clinical symptoms from thyroid dysfunction, but 9 patients were given thyroid hormone after examination of thyroid function test. TSH elevation rate for 95 patients irradiated to the hole neck by dosage was 0%, 24%, 47% and 47%, for less than 30 Gy, 30-39 Gy, 40-49 Gy and 50 Gy or more, respectively. The effects of lymphangiography and systemic combination chemotherapy on the thyroid function were also discussed.

Adolescent↗
PubMed1987

[Osteonecrosis following radiotherapy in carcinoma of the uterus and pediatric tumors].

Osteonecrosis following radiation therapy in 611 Patients with uterine cancer and 41 patients with pediatric tumor were studied. The 5- and 10-year cumulative bone injury rate after radiation therapy in uterine cancer was 4% and 15.6%, respectedly. Patients who are older than 60 years of age showed high occurrence of radiation bone injury. Otherwise, severe aletrations in shape and complete arrest of growth in irradiated growing bone were observed of even with small doses of 1600 cGy in the lower age level (0-3 age of years) at the time of treatment.

Adolescent↗
PubMed1987

[Factors influencing the response of normal tissue to radiation].

The dose modifying effects of the age, anatomic location of the normal tissue, positioning of animal during irradiation, preliminary treatment, number of fractions and time interval between doses on response of the normal tissues to radiation were studied, using various kinds of response of mouse hind legs such as early skin reaction, epilation, skin contraction, ulcer formation, leg contraction and growth retardation of femur. The dose modifying factors varied with the kinds and the levels of injuries, meaning that it is very important to define what the critical normal tissue is and its end point first of all, when we discuss the dose modifying factors with respect to the therapeutic gain factors.

Alopecia↗
PubMed1986

Response of previously irradiated mouse skin to a second course of irradiation: early skin reaction and skin shrinkage.

The responses of previously irradiated hind legs of mice to second courses of gamma ray irradiation were studied using early skin reaction and skin shrinkage as end points. The hind legs of mice were treated with various doses in single and multifractionated irradiation at one-day intervals, 12 months after a variety of doses in various treatment schedules and in a single exposure, respectively. The re-irradiated skin was relatively radioresistant compared to the previously non-irradiated skin, depending on the skin site used and the end point used to evaluate the skin's radiation sensitivity. Early skin reactions that occurred after test doses administered to pre-irradiated skin developed sooner, and remained longer, than skin reactions that developed in the previously non-irradiated controls, regardless of treatment regimens. The degree of early skin reaction to the test dose was greater in the pre-irradiated skin, when the test dose was relatively small, and lower, when the test dose was relatively large, regardless of treatment regimens with one exception: the ventral aspect of the mouse's hind leg responded more to multifractionated test doses in the pre-irradiated skin. The degree of skin shrinkage, assessed 50-60 days after the test dose, was less in the pre-irradiated skin than in the previously non-treated controls, for each dose in each test treatment schedule. The amount of skin shrinkage, resulting from a given test dose (25 or 40 Gy), decreased with increasing dose during the first course of each treatment schedule. The degree of skin shrinkage caused by the first and second exposure, however, increased when the total dose of the first course and the test exposure was increased.

Animals↗
PubMed1986

Age dependency of response of the mouse skin to single and multifractionated gamma irradiation.

The age dependency of the response of the mouse skin to gamma-ray irradiation was studied using early skin reaction as an end point. The isoeffect doses for almost all the given levels of early skin reaction were, even though statistically not significant, approximately 10% larger on the average for 38- and 64-week-old mice than for 12-week-old mice. No significant difference between 12-week-old mice and 64-week-old mice was observed in the proportion of dose recovered during multifractionated exposures and in the slope of the fraction number and isoeffect dose relationships plotted on a log-log scale, as well as in the effects of anatomical location on the radiosensitivity. A preliminary shaving made the mouse skin relatively sensitive to radiation, and the dose-modifying factor was smaller for 64-week-old mice than for 12-week-old mice.

Aging↗
PubMed1986

Isolation, and structural and chemical characterization of outer sheath carrying a polygonal array from Treponema phagedenis biotype Reiter.

An outer sheath was isolated from Treponema phagedenis biotype Reiter by our previously developed method (Masuda, K., and Kawata, T. 1982. J. Bacteriol. 150: 1405-1413). The isolated outer sheath was observed as a triple-layered, closed vesicle carrying a polygonal array by electron microscopy. The outer sheath was mainly composed of protein (41.0%), phospholipid (38.7%), and carbohydrate (11.0%). Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the isolated outer sheath in the presence of 2-mercaptoethanol (EtSH) gave one major protein band with an apparent molecular weight of about 69,000 and several minor protein bands. On the other hand, in the absence of EtSH, the major protein band disappeared but two new protein bands at positions of molecular weights of about 65,000 and 72,000 appeared. The SDS-PAGE profiles of the minor protein bands did not change with or without EtSH. Sodium deoxycholate (DOC)-solubilized materials from the isolated outer sheath were reassembled into thin membranous sheets carrying a roughly polygonal array upon removal of DOC by dialysis against Tris-HCl buffer in the absence of Mg2+.

Bacterial Proteins↗