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Biomedical subjects

K Maeda

Publications and source records attributed to K Maeda.

At least 1,189 records · Page 66Linked to original sources
PubMed1988

Positive reactivity of dysplastic melanocytes with a monoclonal antibody against melanoma melanosomes, MoAb HMSA-2.

A mouse-mouse monoclonal antibody, MoAb HMSA-2, was raised against the melanosomal protein of human malignant melanoma. To characterize the nature of dysplastic melanocytic nevi (DMN), we examined the reactivity of DMN with MoAb HMSA-2 in comparison to that of superficial spreading melanoma (SSM) and common melanocytic nevi (CMN) including junctional melanocytic nevi (JMN) on routine paraffin sections. MoAb HMSA-2 showed several unique immunohistochemical findings: a) MoAb HMSA-2 reacted with melanocytes of DMN in both the epidermis and the dermis, including the pigment granules in the keratinocytes; b) the pigment granules in the keratinocytes of DMN were found to be immature melanosomes transferred from dysplastic melanocytes to keratinocytes; c) the reactivity of epidermal melanocytes in DMN and SSM was stronger than that of junctional component in CMN, though SSM revealed a much stronger reaction than DMN; and d) keratinocytes, especially in a "shoulder" lesion of DMN which was associated with dermal lymphocytic infiltrates, often showed a strong reactivity with MoAb HMSA-2. Thus our study suggested a unique immunohistochemical feature of DMN with deranged melanogenesis as indicated by the reactivity with MoAb HMSA-2.

Animals↗
PubMed1988

Role of hypovolemia in dialysis-induced hypotension.

Blood volume was measured continuously during hemodialysis using the authors' hematocrit continuous measurement system in an attempt to determine any relationship between dialysis-induced hypotension with acute onset and blood volume. Hypotension resulted approximately 3 h into dialysis in the present study. This point in time was that at which blood volume was more than 5% above the minimum level during the dialysis treatment, and in some patients it was more than 10% over the minimum level of blood volume. At the time dialysis-induced hypotension occurred or before, there was no sharp decrease in blood volume nor any change in the plasma refilling rate. This suggested that this hypotension is caused by a sudden breakdown of the blood pressure support mechanism compensating for decreased blood volume.

Blood Volume↗
PubMed1988

The ultrasonic Doppler fetal actocardiogram and its computer processing.

Gross fetal movement was detected using a lower frequency ultrasonic Doppler shift than that used in the study of fetal heart action. The movement signal was changed into deflection which was recorded on CTG chart simultaneously with the fetal heart rate (FHR), and was called the fetal actocardiogram. Visual analysis of the actocardiogram showed bursts of active fetal movements in the active fetal state which were concordant with acceleration of FHR. The analysis was useful in the study of fetal behavioural states. Imminent fetal distress produced a loss of FHR acceleration which was accompanied by a burst of fetal movement, i.e. a true non-reactive state of the FHR. The output of the actocardiograph was analysed using a PC98XA computer with a simple statistics of deflection amplitude and interval, displayed as 3-dimensional histograms of the number and frequency, and the formation of the envelope curve of the deflections. The study demonstrated that it is possible to recognize fetal behavioural states automatically. This includes active and resting fetal states as well as fetal breathing movements.

Fetal Heart↗
PubMed1988

Stimulatory effect of histamine on normal human melanocytes in vitro.

Normal human epidermal melanocytes became swollen and more dendritic and the immunoreactive tyrosinase increased markedly when they were cultured for 2 days with 5 microM of histamine. These results suggest that high dermal concentrations of histamine may be responsible for the induction of skin pigmentary changes associated with local proliferation of mast cells such as in urticaria pigmentosa and systemic mastocytosis.

Cells, Cultured↗
PubMed1988

Leukotrienes and thromboxane B2 stimulate normal human melanocytes in vitro: possible inducers of postinflammatory pigmentation.

Normal human epidermal melanocytes became swollen and more dendritic with an increase in the amount of immunoreactive tyrosinase when they were cultured for 2 days with each one of leukotriene (LT) C4, LTD4 and TXB2. Their effects were stronger than that of prostaglandin E2, about which we have previously reported. From these data we suggest that arachidonate-derived chemical mediators, especially LTC4, LTD4 and TXB2 may be responsible for the induction of post-inflammatory hyperpigmentation of the skin.

Cells, Cultured↗
PubMed1988

[Topical application of the controlled release strips containing ofloxacin (PT-01) in periodontal therapy].

The association of periodontopathic bacteria in the subgingival plaque with human periodontal disease has been well established. However, past attempts at reducing the level of pathogenic bacteria by using antibiotics as well as other antibacterial substance, so far, have not been fully successful. In this study, the effect of topical application of ofloxacin (OFLX), a synthetic antibiotic, was evaluated in relation to the clinical parameters. For this purpose, the new developed controlled release strips containing OFLX (PT-01), in which there were structurally immediate- and sustained-releasing portions, were used. 147 adult patients suffering from moderate to severe periodontitis were selected for this study. The patients had received no periodontal treatment previously and had taken no antibiotics within the preceding 6 months. Three different sites with a deep probing pocket depth (greater than or equal to 5 mm) were randomly selected in each patient, and were divided into three groups, i.e., PT-01 applied site (T), placebo-applied site (P) and control site (C). Periodontal treatments consisted of oral hygiene instruction and supragingival scaling on day 0 and 7, and subgingival scaling and root planing on day 14. PT-01 was applied in the periodontal pocket weekly on day 0 to 35, and clinical parameters on each site were recorded weekly. The results showed that, during first 14 days, significant reduction in the percentage of the sites which showed bleeding on probing, pus discharge or mobility of the tooth was observed in the PT-01 applied site. However in placebo and/or control group, no significant change in any parameters was observed in this period. While, after subgingival scaling and root planing, significant improvement was found at all sites in every clinical parameter. Especially, PT-01 applied sites showed significant improvement in the gingival index and bleeding on probing, compared to placebo-applied or control sites. These results suggest that weekly insertion of PT-01 in the periodontal pocket along with the subgingival scaling and root planing could have significant effect on the improvements in the gingival inflammation. Taken together, weekly application of PT-01 might have ameliorating effect as adjuncts of mechanical subgingival plaque control in the periodontal treatment.

Administration, Topical↗