A babesiasis card agglutination test.
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Biomedical subjects
Publications and source records attributed to K L Kuttler.
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Eight adult deer and 4 fawns, from wild populations, were splenectomized and observed for evidence of hemo-parasitic relapses. The animals were maintained indoors under tick-free conditions and in the absence of known arthropod vectors. Following splenectomy 2 adult deer and 2 fawns developed acute Eperythrozoon infections, 3 adult deer developed acute Theileria infections, 2 adult deer developed a demonstrable Anaplasma marginale parasitemia, and 1 adult deer exhibited a low level parasitemia of a plasmodial-like intraerythrocytic parasite. The remaining deer failed to develop a parasitemia following splenectomy. In the absence of a specific parasitemia no drop occurred in packed cell volume following splenectomy.
A complement-fixation (CF) antigen which has been prepared from Theileria infected erythrocytes is capable of reacting to specific serum antibodies of deer acutely infected with Theileria. No sera from 17 deer known to be free of Theileria infection reacted positively to the CF test. Of 35 tests on sera from 12 infected deer having a parasitemia of 2% or less and no accompanying anemia, only 10 (29%) were positive, 2 (6%) were suspicious, and 23 (66%) were negative. Of 65 tests on 8 acutely infected deer, 49 (75%) were positive, 4 (6%) were suspicious and 12 (18%) were negative. Of the 8 deer in which acute theileriasis occurred all reacted to Theileria antigen at one time or another.A significant correlation was found between CF titers and the degree of parasitemia in acute infections. Rabbits were hyperimmunized using erythrocytes from either normal or Theileria infected deer. Reciprocal absorption of the hyperimmune sera with Theileria and normal erythrocytic antigens demonstrated the presence of antibodies specific for Theileria.
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