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Biomedical subjects

K Konno

Publications and source records attributed to K Konno.

At least 469 records · Page 26Linked to original sources
PubMed1981

Cytoplasmic microtubules of rat ascites hepatoma cells.

Cytoplasmic microtubules of 4 strains of rat ascites hepatoma cells including YS, AH 66F, AH 130 and AH 100B were investigated electron microscopically. Microtubules were clearly demonstrated when the cells were fixed at 20 degrees C or 37 degrees C and stained by tannic acid. Morphology, localization and volume density (AH 130 greater than AH 66F greater than YS greater than AH 100B) of microtubules were examined comparatively in these 4 strains and correlation between microtubules and cell deformability was discussed.

Animals↗
PubMed1981

Prophylaxis of 3-methylcholanthrene-induced tumorigenesis in mice with the yeast polysaccharide preparation.

Prophylaxis of 3-methylcholanthrene-induced epithelial tumor formation in ddI mice with our Candida utilis glucomannan preparation (YPS) was confirmed further in the present system of fibrosarcoma production. A single subcutaneous dose of MCA (0.5 mg per mouse) was either preceded by or followed by 10 or 30 daily intraperitoneal (i.p.) injections of YPS at the dosage of 100 mg/kg per dose according to various time schedules. Protective effect of YPS was demonstrable by a significantly lengthened latent period before the tumor appearance and a slower rate of the subsequent tumor production until around day 100. All the schedules of pre- or post-treatment were found to be effective, although the one delayed until two weeks post MCA was so only marginally. It was also disclosed by prolonging the observation period that this protection was of a transient nature, 100 per cent tumor incidence being found by around 150 days post MCA in all the experimental groups. A longer latency due to the treatment with YPS was generally found in association with a faster growth of the tumor and a shorter life span of the host animal. Temporary protection of MCA-induced tumorigenesis in ddI mice was not reproducible in C3H/He mice, and was thus suggested the possible strain-dependent nature of YPS effect.

Adjuvants, Immunologic↗
PubMed1981

Effect of isoproterenol on regional pulmonary perfusion and its blockade by propranolol.

Effects of isoproterenol and propranolol on regional pulmonary perfusion were assessed in the dog. In each experiment the right upper lobe was isolated in vivo by a balloon catheter and artificially ventilated with nitrogen, air or 60% oxygen in nitrogen before and after administration of the medicaments listed above. The rest of the lungs kept breathing air spontaneously. Following administration of isoproterenol regional pulmonary perfusion increased in the hypoxic right upper lobe induced by artificial ventilation with nitrogen, but the increase was not observed when isoproterenol was administered following pretreatment with propranolol. Administration of propranolol alone did not induce any change in regional pulmonary perfusion distribution. When artificial ventilation was done in the right upper lobe either with air or 60% oxygen in nitrogen, thus inducing regional less hypoxia or hyperoxia, no change in regional perfusion was observed with any of the medication. Thus isoproterenol reversed regional hypoxic pulmonary vasoconstriction, but its action was blocked by pretreatment with propranolol. Propranolol per se, however, showed no effect on pulmonary vascular responses to the different alveolar oxygen tensions.

Animals↗
PubMed1981

Effect of dopamine on regional pulmonary perfusion.

Dopamine effect on regional pulmonary perfusion in the right upper lobe was studied in 6 dogs in which the lobe was isolated by a balloon catheter and artificially ventilated with nitrogen, air or 60% oxygen in nitrogen, while the rest of the lungs was allowed spontaneous air respiration. Dopamine was administered at the rate of 20 micrograms/kg/min. Each dog served as its own control. There was no change in the regional vascular responses to the different alveolar oxygen tensions in the right upper lobe before and after dopamine administration.

Animals↗
PubMed1981

Effect of DL-alpha-tocopherol (vitamin E) on the differentiation of mouse myeloid leukemia cell.

The effect of DL-alpha-tocopherol on the differentiation of the mouse myeloid leukemia cell line (M1) was investigated using fluorometry. The reliability of the fluorometric determination was verified by the mutual overlapping of the curves of disappearance of the radioactivity recovered as alpha-tocopherol and the loss of its fluorescence. alpha-Tocopherol solubilized into the culture medium was relatively stable (t1/2 = 147-168 hr). In corporation of alpha-tocopherol into M1 cells was time- and dose-dependent. Proliferation of M1 cells was never inhibited, but was slightly enhanced by treatment with alpha-tocopherol. alpha-Tocopherol caused a twofold increase in acid-phosphatase activity and slightly inhibited both the spontaneous- and dexamethasone-induced differentiation with respect to adhesion on glass and cell surface-rosette formation. alpha-Tocopherol also induced morphological change, making the shape of M1 cells rounder.

Animals↗
PubMed1981

Hypotensive and cardiovascular action of budralazine in unanesthetized and unrestrained dogs.

In renal hypertensive dogs, 1-[2-(1,3-dimethyl-2-butenylidene)hydrazino]phthalazine (budralazine) at doses of 1 and 2.5 mg/kg p.o. produced a gradual, progressive and significant fall in blood pressure which reached a maximum 3--4 h after dosing and persisted for 6--10 h. The hypotensive response to 1 mg/kg p.o. of budralazine was almost comparable to that to 2.5 mg/kg p.o. of reference drug hydralazine, but the duration of action of the former was much longer than that of the latter. At equihypotensive doses, budralazine was found to be approximately 3 times less potent than hydralazine in producing tachycardia. In normotensive dogs, budralazine at a dose of 1 mg/kg p.o. produced a significant increase in renal blood flow and a tendency for the increase in coronary blood flow associated with a significant and non-significant decrease in the peripheral vascular resistance. At this dose level, blood pressure remained almost unchanged.

Animals↗
PubMed1980

ATP-induced tension development in glycerinated fibers of scallop adductor striated muscle. Role of regulatory light chain of myosin in calcium regulation of muscle contraction.

Removal of the regulatory light chain subunit (EDTA light chain) of myosin from glycerinated fibers of scallop adductor striated muscle resulted in an immediate loss of calcium sensitivity of tension development and in a subsequent decrease in tension developed in the presence of calcium. It is suggested that removal of EDTA light chain results in a change in the myosin heavy-chain conformation which is probably responsible for the decrease in tension development.

Adenosine Triphosphate↗
PubMed1980

Intraventricular hemorrhage in childhood moyamoya disease.

A 7-year-old female with computed tomographic evidence of intraventricular hemorrhage was subsequently proven by cerebral angiography to have moyamoya disease. The rarity of this manifestation of moyamoya disease and its cause are discussed. In addition to cerebral atrophy and old focal infarctions, the demonstration of tortuous, irregular vessels in the basal ganglia and poor visualization of the proximal portions of the anterior and middle cerebral arteries should suggest moyamoya disease before cerebral angiography is performed.

Arterial Occlusive Diseases↗
PubMed1980

Effect of non-filtered and filtered cigarette smoke on mucociliary clearance mechanism.

Acute effect of a short-term exposure to cigarette smoke on mucociliary clearance mechanism was studied in 69 anesthetized dogs. A tracer material, 99mTc-MAA (macroaggregated albumin), was placed in the right main bronchus near the carina 2 min after completion of exposure and its migration distance on the tracheal mucosa with time was assessed by a scintillation camera, and the mean migrating velocity (MMV) was calculated. The average MMV values were 12.0 +/- 1.0 (mean +/- S.E.) mm/min in the control dogs, while the values were 11.6 +/- 1.5, 8.3 +/- 2.2, 3.3 +/- 0.8, and 4.2 +/- 2.3 mm/min in the dogs which smoked 1, 3, 5 and 8 cigarettes, indicating that there was an inverse correlationship between the number of cigarettes smoked and the MMV values. When cigarette smoke was filtered through a glass fiber filter, the average MMV values were 7.0 +/- 0.5 and 1.3 +/- 0.9 mm/min in the dogs which smoked 5 and 8 cigarettes respectively, suggesting that once the number of cigarettes amounted to eight, even cigarette smoke filtered through a glass fiber filter was as detrimental to the mucociliary clearance mechanism as the non-filtered cigarette smoke.

Animals↗
PubMed1980

Effect of alveolar hypercapnia on regional pulmonary perfusion.

The effect of alveolar hypercapnia under a wide range of alveolar oxygen tensions was assessed in the right upper lobe of the normal dogs and the dogs with the reimplanted right lung. To induce alveolar hypercapnia by artificial ventilation of the right upper lobe, gas mixtures of differing oxygen fractions containing 10% or 20% CO2 were used as exchange gases for the right upper lobe. The concurrent alveolar hypercapnia had little superimposed effect on the regional vascular responses to a wide range of alveolar oxygen tensions in the normal and the reimplanted denervated lung lobe. Under an extreme alveolar hyperoxia, however, the concurrent alveolar hypercapnia somewhat suppressed regional hyperoxic vasodilation and/or recruitment of the pulmonary vascular beds, whereas in the reimplanted lung lobe it potentiated the hyperoxic pulmonary vascular response. The alveolar oxygen tension seems to be the main factor in the regulation of regional perfusion in the lungs.

Animals↗