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Biomedical subjects

K Kin

Publications and source records attributed to K Kin.

At least 19 recordsLinked to original sources
PubMed1992

[Ocular penetration of UF-021, a new prostaglandin related compound, in the rabbit eye].

The ocular penetration and pigment affinity of topically applied UF-021, a newly developed prostaglandin-related compound, were investigated in rabbit eyes using 3H-labeled UF-021. Ten minutes to 24 hours after instillation of 0.12% of 3H-UF-021 solution into New Zealand white or pigmented rabbit eyes, the rabbits were sacrificed and the eyes were immediately enucleated. The extraocular muscles, conjunctiva, aqueous humor, cornea, iris, anterior and posterior sclera, ciliary body, lens, vitreous, retino-choroid complex, optic nerve were separated and blood samples were taken. The concentration of 3H-UF-021 in each sample was determined with a liquid scintillation counter. The peak concentration of UF-021 was obtained at 40 minutes and 1 hour after instillation in the iris-ciliary body and aqueous humor of albino rabbit. According to the pharmacokinetic analysis of the data, UF-021 showed good ocular penetration into the rabbit eye, and the permeability of the epithelial barrier for UF-021 was calculated to be 2.9 x 10(-3) cm/hr. The apparent elimination rate constant and the apparent absorption rate constant were also calculated to be 0.21 hr-1 and 1.28hr-1, respectively. The time course for 3H-UF-021 concentration in the eye of pigmented rabbit was almost the same as that in the eye of non-pigmented rabbit, indicating that UF-021 did not bind to pigmented tissues.

Animals↗
PubMed1992

[A mechanism for reducing intraocular pressure in normal volunteers using UF-021, a prostaglandin-related compound].

The mechanism of reduction of intraocular pressure (IOP) and other ocular effects were studies after topical application of prostaglandin (PG) F2 alpha and UF-021, a new PG related compound, in eight normal volunteers. IOP, aqueous humor flow rate and outflow rate were evaluated during a period of four hours after the application. Both PGF2 alpha and UF-021 caused significant and similar IOP reduction for four hours. Neither compound produced any significant change in the aqueous humor flow rate or outflow rate, suggesting the increase of unconventional outflow rate as being the possible mechanism of IOP reduction in normal human eyes.

Adult↗
PubMed1991

Bone mineral density of the spine in normal Japanese subjects using dual-energy X-ray absorptiometry: effect of obesity and menopausal status.

Bone mineral density (BMD) of the lumbar spine was measured to determine normal Japanese values and to examine the effect of obesity and menopausal status on BMD. Normal Japanese subjects (N = 1,296, 1,048 women and 248 men) were examined using dual-energy X-ray absorptiometry. BMD for men peaked between age 20 and 29. For women, there was abrupt bone loss after age 50. Obese women within the same age bracket had a higher BMD than thin women after age 40-49. We determined that BMD began to decline during the irregular menstruation period before the onset of menopause. We conclude that there is a positive correlation between obesity and BMD, particularly in postmenopausal women. In addition, we found that bone loss related to menopause begins during the irregular menstruation period before menopause.

Absorptiometry, Photon↗
PubMed1991

[An adult case of the left coronary artery anomalously originating from the pulmonary artery (BWG syndrome), demonstrating remarkable improvement in postoperative thallium-201 myocardial imaging].

Stress thallium-201 myocardial scintigraphy were performed in a 44-year-old female with BWG syndrome before and after aorto-coronary bypass surgery. Preoperative stress thallium-201 studies demonstrated anterior reversible perfusion defect and posterior persistent defect. But postoperative studies demonstrated no defects in both areas. These data suggest that preoperative anterior defect was due to transient myocardial ischemia, but the finding of posterior persistent defect wasn't due to infarction or scarring. Thus, stress thallium-201 myocardial imaging provided useful informations to clarify the myocardial perfusion patterns pre- and postoperatively.

Adult↗
PubMed1984

Release of slow reacting substance from rabbit ocular tissues.

Investigations were carried out to determine whether any slow reacting substance (SRS) is released from the ocular tissues. The SRS activity was bioassayed using the guinea pig ileum in the presence of mepyramine and atropine. An SRS-specific antagonist, FPL55712, was also used to confirm SRS-induced ileum contraction. Incubation of the ocular tissue fragments of the rabbit with a Ca-ionophore A23187 resulted in a marked release of the SRS in the potency order of the choroid, ciliary body, conjunctiva and iris. The rabbit was sensitized with bovine serum albumin and complete Freund's adjuvant. Experimental uveitis was induced by injection of antigen into the vitreous body the sensitized rabbits. The SRS could not be detected in the aqueous humor 10-24 hours after the antigen injection but it was released markedly into the vitreous. The SRS from the vitreous humor of the rabbits with experimental uveitis was purified by high performance liquid chromatography, and leukotriene C4 and leukotriene D4 were identified. It was suggested that release of the SRS from the ocular tissues plays a role in allergic and nonallergic uveitis.

Animals↗
PubMed1980

Human alpha 1-microglobulin levels in various body fluids.

alpha 1-Microglobulin levels in serum and urine were estimated by using single radial immunodiffusion, resulting in the following mean values: umbilical cord blood serum, 40.6 mg/l; normal adult serum, 44.2 mg/l; and normal urine, 5.7 mg/24 h urine volume. Slightly higher levels of serum alpha 1-microglobulin were found in infants and the aged. Serum and urine alpha 1-microglobulin levels in patients with renal failure, however, were greatly increased, mean levels being 231.5 mg/l and 100.7 mg/24 h urine volume, respectively. Serum alpha 1-microglobulin levels in these patients correlated well with both serum creatinine and beta 2-microglobulin levels. Serum alpha 1-microglobulin level did not correlate positively with serum levels of other plasma proteins, such as alpha 1-antitrypsin, haptoglobin, complement, etc. Ouchterlony immunodiffusion also revealed the presence of alpha 1-microglobulin in synovial fluid, ascites, pleural effusion, amniotic fluid, cyst fluid, and cerebrospinal fluid. The levels of alpha 1-microglobulin in these fluids were measured by single radial immunodiffusion, except that its level in cerebrospinal fluid was measured by radioimmunoassay. Mean alpha 1-microglobulin concentration was 20.8 mg/l in synovial fluid, 28.7 mg/l in ascites, 21.5 mg/l in pleural effusion, 2.7 mg/l in amniotic fluid, 8.2 mg/l in cyst fluid, and 42.3 ng/ml in cerebrospinal fluid.

Alpha-Globulins↗
PubMed1979

Tissue distribution of human alpha1-microglobulin.

Human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. The carbohydrate content was 21.7%. Anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. Sera from the domestic chicken, mouse, rat, rabbit, dog, calf, cow, goat, sheep, and horse, however, did not react to anti-alpha(1)-microglobulin serum in immunodiffusion. The lymphocyte culture supernate was found to contain alpha(1)-microglobulin. Both thymus-derived(T)- and bone marrow-derived(B)-lymphocyte culture media clearly displayed a specific precipitin line against anti-alpha(1)-microglobulin serum when tested with the Ouchterlony immunodiffusion method. The tissue distribution of alpha(1)-microglobulin was studied under immunofluorescence, and a positive staining was recognized on the lymphocyte surface. Identical staining patterns were noted on both T and B lymphocytes, though B lymphocytes took a more intense stain. It would thus seem quite possible that lymphocytes are the primary source of alpha(1)-microglobulin and that this is filtered through the glomerular basement membrane and partly reabsorbed by the renal tubules. This, then, would suggest the possibility that alpha(1)-microglobulin shares some immunological role in vivo with lymphocytes and(or) is one of the membrane proteins of lymphocytes.

Alpha-Globulins↗