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Biomedical subjects

K Kashima

Publications and source records attributed to K Kashima.

At least 307 records · Page 17Linked to original sources
PubMed1988

Morphometric study of the rat exocrine pancreas after diversion of bile and pancreatic juice from the intestine.

The effects of 6 h of diversion of bile and pancreatic juice from the intestine on the ultrastructure of rat pancreatic acinar cells were studied by morphometric procedures. After diversion the volume of zymogen granules decreased markedly, representing a reduction of 77%. The volume of autophagic vacuoles increased twofold after diversion. The volume of rough endoplasmic reticulum and Golgi apparatus was not different from that of control. It has been proved by electron microscopic morphometry that the degree of reduction of zymogen granules was similar to that after stimulation by exogenous cholecystokinin or introduction of trypsin inhibitor to the duodenum. This suggests that hypersecretion caused by diversion is due to failure of a negative feedback regulation normally induced by proteases found in pancreatic juice. An increased number of autophagic vacuoles suggests an increase of organelle turnover due to heightened secretory activity of acinar cells.

Animals↗
PubMed1985

Effect of intraluminal bile on the feedback regulatory mechanism of pancreatic enzyme secretion in conscious rats.

The effect of intraluminal bile on the well-known feedback regulatory mechanism of exocrine pancreatic secretion exerted by intraluminal trypsin was investigated in conscious rats with pancreatic, biliary and duodenal fistulae. The stimulated pancreatic enzyme secretion caused by diversion of bile-pancreatic juice from the intestine was apparently suppressed by intraduodenal reintroduction of pancreatic juice or bile-pancreatic juice, while it was slightly suppressed by intraduodenal reintroduction of bile. Although additional reintroduction of bile did not alter the already suppressed pancreatic enzyme secretion by the presence of pancreatic juice in the intestine, diversion of bile stimulated the suppressed pancreatic enzyme secretion by intraluminal bile-pancreatic juice. Infusion of sodium taurocholate into the duodenum with diversion of bile-pancreatic juice effectively inhibited pancreatic enzyme secretion. The inhibitory effect seemed to be dependent on the concentration of taurocholate infused into the duodenum. The results suggest that bile and bile acid have an important role in the feedback regulatory mechanism of pancreatic enzyme secretion, at least partly directly inhibiting the secretion.

Amylases↗
PubMed1984

Radioimmunoassay of guanabenz, an antihypertensive agent.

Antibodies with a high specificity and sensitivity for [(2,6-dichlorobenzylidene)amino]guanidine acetate (guanabenz, GB), a potent antihypertensive agent, were produced in rabbits immunized with GB conjugated to bovine serum albumin. GB-specific antibodies were detected 6-8 days after the first booster injection and the titer steadily increased over the initial 17-25 weeks of immunization. A high degree of specificity was demonstrated. No metabolite of GB detected in humans or several animal species cross-reacted, even when present in over 100-fold excess. A rapid and convenient radioimmunoassay procedure for GB was designed using these antibodies. Competition between tritiated GB tracer and unlabeled GB for specific antibody binding sites made feasible measurement of as little as 30 pg GB. GB was absorbed rapidly from the gastrointestinal tract and reached a maximum level in the plasma (2.50 +/- 0.45 ng/ml) at 2 h after oral administration (1 mg/kg) to dogs.

Animals↗
PubMed1979

Mass cells in ocular tissues of normal rats and rats infected with Nippostrongylus brasiliensis.

In orbital exenteration specimens from 14 rats, 93% of the mast cells were found in the lids, the limbus, and the conjunctiva, 5% in the orbital tissues, and less than 1% in the globe. The density of mast cells was highest in lid (2843/mm3), limbus (2822/mm3), and orbit (2184/mm3) and lowest in bulbar conjunctiva (794/mm3), ciliary body (512/mm3), and sclera (176/mm3). There was no significant difference in the distribution or density of mast cells in orbital exenteration specimens from normal rats compared with rats infected with the worm Nippostrongylus brasiliensis. We concluded that certain ocular structures are rich in mast cells, which suggests that these structures might be susceptible to injury mediated by mast cell products.

Animals↗
PubMed1979

IgG in human cornea stained by various techniques.

Four eyes were divided into portions which were prepared by various techniques for immunofluorescence microscopy. One portion was frozen and processed by cryostat cutting, a second was fixed in alcohol-acetic acid and embedded in paraffin, and a third was soaked in saline for 2 days to elute IgG and was then processed by the alcohol-acetic acid technique. All tissues received stain for IgG by either the direct (one-layer) or indirect (two-layer) technique. In all instances IgG was demonstrated in the same areas of the cornea. We conclude that the presence of IgG in human cornea is not dependent on the method used for fixation or processing of the tissue.

Aged↗