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Biomedical subjects

K Iinuma

Publications and source records attributed to K Iinuma.

At least 217 records · Page 12Linked to original sources
PubMed1977

Pitfalls in the use of chromosome variants for paternity dispute cases.

A huge 'satellite' (22s+) was observed in a mentally retarded and malformed girl. It consisted of constitutive heterochromatin based on the C-band technique (CBG). In neither of the parents was the variant observed. Genetic marker studies revealed that the father was, indeed, the father of the proposita (probability, 99.8%). It is emphasized that caution must be taken in the use of chromosome variants for paternity dispute cases.

Abnormalities, Multiple↗
PubMed1977

[A simple method measuring plasma aldosterone by radioimmunoassay without extraction (author's transl)].

A radioimmunoassay method was developed to measure plasma aldosterone levels. Antibody was produced in rabbits by injecting aldosterone oxime coupled with bovine gamma-globulin once a month. Plasma aldosterone was measured simultaneously by two methods: the direct method without extraction and a method using paper chromatography. 125I-aldosterone was used in the first method and 3H-aldosterone in the second. The antibody had a high specificity adequate to show zero water blank in the first method. Adequate precision, accuracy and sensitivity were obtained in a direct method using 125I-aldosterone. Plasma aldosterone levels were 7.1+/-3.0ng/100ml (Mean +/-SD) in normal subjects and slightly higher after injecting ACTH-Z. The correlative coefficient between the first and the second method was significantly high (r=0.970, P less than 0.001, n=37). Plasma aldosterone was high (34.3+/-14.1ng/100ml, n=7) in primary aldosteronism, slightly high (14.2+/-2.6ng/100ml) in secondary aldosteronism, normal in Cushing's syndrome (10ng/100ml) and low in Addison's disease (1ng/100ml), hypopituitarism (1ng/100ml) and pseudoaldosteronism (2ng/100ml). From these results, it is concluded that the direct method without extraction was a very useful and reliable method for measuring plasma aldosterone. It was superior in simplicity and there is no need to use a liquid scintillation counter.

Addison Disease↗
PubMed1977

A case report of a presumptive +i(18p) associated with serum IgA deficiency.

The case of a 4-month-old male infant with retarded psychomotor development and multiple anomalies is presented. Cytogenetic studies on peripheral blood and skin cultures revealed a normal male complement with a supernumerary small metacentric chromosome. According to its size and its banding patterns, the metacentric chromosome was postulated to be an isochromosome for the short arm of number 18. A deficiency of serum IgA was observed in this patient.

Abnormalities, Multiple↗
PubMed1977

A non-chromatographic non-extraction radioimmunoassay for serum aldosterone.

A direct radioimmunoassay for serum aldosterone was developed using a highly specific sntibody and 8-anilino-1-naphthalene sulfonic acid as a blocking agent to inhibit the binding of aldosterone to serum proteins. 125I-labeled aldosterone was used as the labeled antigen and polyethylene glycol was used to separate antibody-bound and free aldosterone. The minimum detectable concentration was 1.5 pg/tube. There were excellent correlations between the present method and other methods, i.e., 1) a method using tritiated aldosterone, 2) a method using dichloromethane extraction before assay, and 3) a commercial kit method. The intra-assay coefficient of variation was 6.9%, and the inter-assay coefficient of variation was 10.7%. The values found in normal human serum were comparable with those reported using other methods. The present radioimmunoassay eliminates both extraction of aldosterone from serum and chromatographic separation and requires only 0.1 ml of serum for assay.

Aldosterone↗
PubMed1975

Y-chromatin positive cells in the smear preparations of the gonad from a XX male.

An ambisexual male infant showed a normal female karyotype by the leukocyte culture technique in two different occasions. Cultured fibroblasts from the right gonad had also a 46, XX complement. They were Y-chromatin negative. To our surprise, Y-chromatin was observed in 96% of the nuclei in smears from the uncultured gonad. It is emphasized that the examination of the uncultured gonad by the quinacrine technique is mandatory in the study of "XX males".

Fibroblasts↗
PubMed1975

Electroencephalographic findings in a case of globoid cell leukodystrophy.

An increased slow wave pattern of the EEG basic waves without epileptogenic discharges was observed in an early stage of a case of Krabbe's disease. In the later stage of the illness, spikes and sharp waves were mixed with. The peculiar runs of fast activity which were described by Kliemann et al. (1969) were not observed during the course of our patient.

Electroencephalography↗