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Biomedical subjects

K Iinuma

Publications and source records attributed to K Iinuma.

At least 199 records · Page 11Linked to original sources
PubMed1982

[Gastrin radioimmunoassay with polyethylene glycol method].

125I-labelled gastrin with high immunological activity and high specific radioactivity of 22.2 approximately 25.9 GBq (600 approximately mCi)/mg (0.5 iodine atom/1 gastrin molecule) was prepared by chloramine-T method using the amount of 100 molar ratio of chloramine-T to gastrin. As results of gastrin radioimmunoassay with polyethylene glycol (PEG) method, intra- and interassay coefficient of variation (C.V.) were 3.6 approximately 7.6% and 5.7 approximately 10.4%, respectively. The average recoveries of 2 levels of gastrin added to serum samples were 104 +/- 11.9% and 105 +/- 9.7%. The correlation coefficient of gastrin values obtained with dextrancoated charcoal (DCC) method and present method was r = 0.973. Antibody used in this study have a characteristic to strongly recognize the tyrosin residue at 12 position and the methionyl residue at 15 position on gastrin (1-17).

Antibody Specificity↗
PubMed1981

[A simple solid phase radioimmunoassay for plasma renin activity (author's transl)].

A solid phase radioimmunoassay (RIA) in which plasma renin activity (PRA) can be measured in a single test tube throughout enzymatic reaction and determination of angiotensin-I (A-I) generated was developed. Two hundred micro 1 of plasma samples was incubated with phenylmethylsulfonyl fluoride (PMSF) and ethylenediamine tetraaceticacid (EDTA) at pH 6.0 or 7.4 at 37 degrees C for 1 hr, then the generated A-I was determined by solid phase RIA under 3 hr of incubation time at room temperature. An antibody absorbed onto polystyrene beads was used for B/F separation of RIA. The combination of PMSF and EDTA gave significant inhibition of angiotensinase and converting enzyme. Pepstatin A, proteolytic enzyme inhibitor, was found to be effective to block the renin activity during the incubation for RIA at room temperature. Recoveries of A-I added at two levels of 1.0 4.0 ng/ml to plasma sample incubated at 37 degrees C for 1 hr were 102 +/- 28.6% and 105 +/- 15.2%, respectively. Intra-assay precision (CV) ranged from 2.5 to 10.3% and inter-assay from 7.0 to 13.7% with four pooled sera. PRA obtained under enzymatic reaction of pH 6.0 showed approximately twice as much as that of pH 7.4. The assay correlated closely with the other established solid phase radioimmunoassay kit (r = 0.996). Normal values of PRA were 1.03 +/0 0.78 ng/mg/hr (n = 66) at supine position and 1.33 +/- 1.15 ng/ml/hr at upright position. The method for the measurement of PRA, described in this paper, can be clinically used as one of convenient methods froms its simplicity and reproducibility.

Female↗
PubMed1981

Mortality and survival for Down syndrome in Japan.

Mortality and survival data for 1,052 Japanese patients with Down syndrome who were born between 1966 and 1975 were analyzed. The survival rate at age 10 was estimated to be about 86%. Mortality in each age group for Down syndrome was elevated over that of the general population. In the survival rate at age 10, there was no significant difference between males and females, but the difference between cases with and without congenital heart disease was highly significant. Using data from this study-for mortality up to age 10-and from the study of institutionalized cases for mortality over age 10, a hypothetical life table was constructed; it shows that the life expectancy at birth for cases with Down syndrome is nearly 50 years.

Adolescent↗
PubMed1980

Dihydropteridine reductase deficiency: diagnosis by leukocyte enzyme assay.

An assay procedure for dihydropteridine reductase in peripheral leukocytes is described. The assay utilizes the tetrahydropterin-dependent reduction of ferri-cytochrome C in the presence of NADH and requires a smaller number of cells than assays described for cultured skin fibroblasts. Dihydropteridine reductase activity was not detectable in the peripheral leukocytes nor in the cultured skin fibroblasts from two adolescent patients with malignant hyperphenylalaninemia. The parents of the patients showed approximately 50% of normal dihydropteridine reductase activity in their peripheral leukocytes. Immunochemical experiments using antibodies against bovine liver dihydropteridine reductase suggest that normal leukocytes and skin fibroblasts contain dihydropteridine reductase which is immunologically similar to that of human liver. The present studies indicate that the determination of dihydropteridine reductase activity in peripheral leukocytes can be used to diagnose hyperphenylalaninemia due to a defect in dihydropteridine reductase.

Amino Acid Metabolism, Inborn Errors↗
PubMed1980

The Coffin-Siris syndrome: a case report.

A 5-month-old female with the Coffin-Siris syndrome is described. Characteristic features included prenatal and postnatal growth retardation, slight mental retardation, feeding and respiratory problems, microcephaly, hypotonia, full lips, congenital heart disease, hypoplasia of the distal phalanges of the fifth fingers and toes. She underwent successful primary closure of a ventricular septal defect at 8.5 months of age, weighing 5.0 kg. After the operation, the feeding and respiratory problems disappeared, and growth and development were well maintained.

Abnormalities, Multiple↗
PubMed1979

[A simplified direct radioimmunoassay for urinary aldosterone (author's transl)].

A simplified direct radioimmunoassay for urinary acid labile aldosterone was developed. One ml of urine was hydrolysed with 2 ml of 0.2N HCL at 30 degrees C for 16hrs. One tenth ml of hydrolysed urine diluted 10 times with charcoal treated aldosterone-free calf serum was used for the radioimmunoassay. The radioimmunoasssay was done with a specific antibody, 125I-aldosterone, as the labeled antigen and polyethylene glycol for bound-free separation. There were excellent correlations between the present methods and other methods, i.e., i) a method using dichloromethane extraction before the assay as well as pre-extraction before hydrolysis and ii) a commercial kit using 3H-aldosterone. The intra-assay coefficient of variation was 5.8%, and the inter-assay coefficient of variation was 9.5%. The normal value of urinary aldosterone was excretion was 3.7 plus or minus 2.5 micrograms/day by the present method, and values of patients with primary aldosteronism were between 24 to 43 micrograms/day.

Aldosterone↗
PubMed1979

Hajdu-Cheney syndrome.

Hadjdu-Cheney syndrome is a rare type of syndrome characterized by acro-osteolysis, dolichocephaly with multiple Wormian bones, absence of frontal sinuses and joint laxity. A case of this syndrome is presented. A histological study of the osteolytic lesion revealed destruction characterized by microfractures with a poor reparative process. It is postulated that an abnormality of osteoblast or osteoid function is the pathogenesis of this syndrome.

Abnormalities, Multiple↗
PubMed1979

Childhood epilepsy uncontrolled by phenytoin: clinical and electroencephalographic study.

Nine cases of childhood epilepsy manifesting motor convulsions uncontrolled despite high levels of phenytoin (PHT) were studied clinically and electroencephalographically. These cases consisted of five cases of partial seizures without impairment of consciousness, two cases of partial seizures (occasionally generalized seizures beginning locally), one case of predominantly unilateral seizures, and one case of generalized tonic-clonic seizures. the onset of seizures was at a rather early age, between 3 months and 9 years, and under 3 years of age in eight cases. All cases had single or multiple, cortical epileptic foci in EEG. The projection of spikes was localized to a rather limited area. Seizures of these patients were frequent. All cases, except one, did not respond to other medication. Convulsive seizures with cortical focal spike foci in EEG uncontrolled despite high levels of PHT were thought to have poor responsiveness to not only PHT itself, but also to other anticonvulsants.

Adolescent↗
PubMed1978

Immediate changes of the electroencephalograms after intravenous injection of clonazepam and their relation to its effect on clinical fits in children with minor seizures.

Immediate EEG changes after intravenous administration of clonazepam and a correlation between the EEG changes and the effect of oral administration of the drug were studied in 21 children with minor seizures whose interictal EEG showed a paroxysmal abnormality. In 13 cases of infantile spasms whose EEG showed hypasrhythmia, paroxysmal discharges were completely or remarkably suppressed in 4 cases, partially suppressed in 3 cases, but not improved in 6 cases. Suppression bursts pattern was less improved. In 5 cases of Lennox syndrome, paroxysmal discharges were markedly improved in 3 cases. In a case of petit mal absence, parxoxysmal discharges were completely suppressed. In all 5 cases whose EEGs were completely improved, paroxysmal discharges reappeared 7 to 30 min after the intravenous injection. In 2 out of the 5 cases, paroxysmal discharges became severer at reappearance than before the injection. Among 12 cases whose EEG showed an improvement after the intravenous injection, their clinical seizures were improved in 9 cases, but the clinical effect was mostly transient. In the majority of the cases whose EEGs were not improved, no clinical effect was observed. There was a highly significant correlation between immediate EEG changes and clinical effect of clonazepam (p less than 0.02 by the chi-square test).

Administration, Oral↗