Search PubMed⌕ Search

Biomedical subjects

K Ida

Publications and source records attributed to K Ida.

At least 145 records · Page 8Linked to original sources
PubMed1979

[An experimental study on the pathogenesis of osteoarthritis--histological and biochemical changes of proteoglycan in the osteoarthritic cartilage of rabbit in the early stage-- (author's transl)].

Osteoarthritis is generally a slowly progressive destructive disease of joints. As the disease is asymptomatic in its initial phases, it was detectable only at the later stage when radiological changes and clinical symptom had developed. The biochemical and cellular changes that had been recorded were those of the later clinically recognizable stage of the disease. Because the time of onset in not known, the initial events which might be the most important to investigate the pathogenesis of osteoarthritis, are virtually impossible to investigate except in experimentally induced models of osteoarthritis. For the purpose of investigating the pathogenesis of degenerative osteoarthritis, experimental osteoarthritic model was prepared. Section of the medial collateral and both cruciate ligaments combined with resection of the medial meniscus in adult rabbit knees showed progressive histological changes similar to those in human osteoarthritis. Therefore, qualitative and progressive alteration of the cartilage matrix proteoglycan in experimental osteoarthritis of the early stages were investigated with time. Biochemical analysis of the cartilage from experimental osteoarthritic model demonstrated a decrease in the content of glycosaminoglycan and increase in the rate of the synthesis of glycosaminoglycan as compared to the normals. The analysis of disaccharide chain revealed a decrease in the concentration of kelatan sulfate and chondroitin-6 sulfate, along with an increase in the concentration of chondroitin-4 sulfate. These findings, which were quite consistent with those in human osteoarthritis and were contrary to those in aging, indicate the attempts of cells to compensate for the proteoglycan deficiency in osteoarthritic cartilage by synthesizing large quantities of glycosaminoglycans of immature pattern. On the other hand, synthesized aggregated proteoglycans in arthritic cartilage had shown a polydispersive pattern in CsCl density gradient compared to that of normal one, which had shown a single curved pattern. Further, newly synthesized proteoglycan in osteoarthritic cartilage seemed to be polydispersive compared with control proteoglycan in molecular sizes on Sepharose 2B gel chromatography under non-aggregated dissociative condition. Large molecular size proteoglycan composed of small molecular size glycosaminoglycan on its polysaccharide chain and small molecular size proteoglycan composed of large molecular size glycosaminoglycan. This might represent the expression of repair mechanism in the damaged cartilage, or phenotypic transformation of extracellular macromolecule in osteoarthritis.

Animals↗
PubMed1976

[Endoscopic electromyographic study on upper gastrointestinal tract (author's transl)].

Recently we made the electromyographic study on human gastrointestinal tract using the endoscopic method is useful for the clinical studies of the patho-physiological aspects on human gastrointestinal tract. The silver bipolar needle electrode which was 0.2 mm in diameter, 10 mm in length and 1.0 mm in interpolar distance and the electroencephalography recorder provided with pen-writer were used for this research. The endoscopy was done to the volunteer anesthetized his pharynx with xylocaine spray. 20 cases of gastro-electromyograms, 35 cases of duodeno-electromyograms and 5 cases of the electromyograms of duodenal papilla were recorded. On these endoscopic electromygorams, the typical rhythms and patterns of electrical activities were disturbed by various kinds of factors; that is, on the endoscopic manipulation, the sticking of needle electrode into the shallow layer of gastrointestinal wall and automatical pouring of water into canal through the endoscope. On volunteers' condition, his deep breathing, electrocardiogram and vasotonic condition were important factors. Typical duodeno-electromyograms showed the grouped spikes synchronizing with the duodenal peristalsis observed endoscopically. The electromyograms of the duodenal papilla showed the similar patterns and rhythms to those of duodeno-electromyograms on active phase. In the clinical study on the correlation and propagation between gastric and duodenal motility, the rhythms of electrical activities on duodeno-electromyograms were not similar to the rhythms of gastric peristalsis at the prepyloric region. The propagation of gastric movement to duodenal bulb were not able to be clarified. We assumed that the gastric and duodenal motility occurred each other on the different rhythms.

Adult↗