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Biomedical subjects

K Harada

Publications and source records attributed to K Harada.

At least 1,225 records · Page 68Linked to original sources
PubMed1980

Clinical evaluation of inside echo patterns in gray scale prostatic echography.

Improved gray scale echograms in transrectal ultrasonography clearly can visualize the anatomical structure of and pathological changes in the prostatic gland. This study includes the classification of ultrasonic findings from the prostatic gland into acoustical pattern groups--solid, cystic and mixed--as well as into pathological-anatomical pattern groups--internal gland, external gland, nodule, stone and miscellaneous. The diagnostic accuracy of prostatic diseases using pattern analysis of prostatic inside echoes was 89 per cent, a 9 per cent improvement over that provided by conventional display.

Calculi↗
PubMed1980

Problems and reconstruction of "dish face" deformity due to cleft lip and palate.

This paper deals with the unique aspects and surgical indication of "dish face" deformity, including "flat face" deformity, due to cleft lip and palate. The importance of deformities of the nose and lip caused by cleft lip and palate and the role of their reconstruction in the treatment of "dish face" deformity are described in detail. For the protruding chin and pseudoprognathism, vertical osteotomy of the ramus of the mandible is recommended over advancement osteotomy of the hypoplastic maxilla.

Cleft Lip↗
PubMed1980

Lymphangiographic demonstration of thoracic duct cyst.

We describe the first reported case of a cyst of the thoracic duct which was diagnosed prior to surgery by bipedal lymphangiographic studies. The differential diagnosis of posterior mediastinal masses should include this pathologic entity.

Adult↗
PubMed1980

Myocardial infarction and left coronary ostial stenosis in infancy simulating anomalous origin of the left coronary artery. A case report.

A case of a 3-month-old male infant with myocardial infarction due to left coronary ostial stenosis is presented. Clinically it was quite similar to anomalous origin of the left coronary artery. Postmortem examination revealed quite narrow left coronary ostium and myocardial infarction with aneurysm of the left ventricular apex.

Coronary Vessel Anomalies↗
PubMed1980

Lipoma of the heart in a child. Clinical, echocardiographic, angiographic, and pathological features.

A 7-year-old boy presented with intermittent systolic murmur and syncope. Chest roentgenogram revealed mild cardiomegaly. Electrocardiogram showed right bundle branch block and abnormal T-waves. Right ventricular angiography demonstrated a filling defect at the anterior portion of the right ventricular cavity and histological examination of biopsy specimen revealed lipoma. At operation, the tumor could not be totally resected. Histological examination of surgical specimen showed marked proliferation of mature fat cells between cardiac muscle fibers. Postoperatively he has been remained asymptomatic. Postoperative standard M-mode and two-dimensional echocardiogram demonstrated a mass from right ventricular wall, abnormal motion of the interventricular septum and small left atrial cavity. The patient has been followed by serial echocardiography.

Angiography↗
PubMed1980

[Immunofluorescent analysis of intracellular localization of estrogen and progesterone in normal human endometrial cells (author's transl)].

Intracellular localization and the heat-dependent redistribution of estrogen and progesterone in human endometrial cells have been investigated by a fluorescent steroid-antibody technique. The dispersed endometrial cells were incubated with 5 X 10(-8) M estradiol-17 beta and progesterone in TC medium 199 containing 10% calf serum for 1--3 hr at 4 degrees C or 37 degrees C. An indirect immunofluorescent technique using FITC-labeled anti-rabbit IgG and steroid antibodies raised from rabbits immunizing with estradiol-6-oxime-BSA and progesterone-3-oxime-BSA was applied to the smear specimens. In normal endometrial cells in both proliferative and secretory stages, specific fluorescences to estradiol and progesterone were generally observed in the cytoplasm after incubation with the steroids at 4 degrees C for 1 hr. When these cells were incubated with the steroids at 37 degrees C for 1 hr, cytoplasmic and predominant nuclear fluorescences were detected, whereas the 3 hr-incubation at 37 degrees C resulted in disappearance of cytoplasmic fluorescence, remaining nuclear fluorescence alone. These fluorescences were remarkedly eliminated when endometrial cells were incubated with diethylstilbestrol and R-5020 prior to the incubation with estradiol and progesterone, respectively. These results indicate that the fluorescent steroid-antibody technique used in this study enables us to visualize subcellular localization of estradiol and progesterone possibly bound to receptors in each endometrial cell.

Endometrium↗
PubMed1980

[Cellular heterogeneity of steroid reactivity in human endometrial carcinoma: an approach by an immunofluorescent steroid-antibody technique (author's transl)].

The dispersed cancer cells obtained from biopsy or surgical specimens of 12 patients with endometrial carcinoma were incubated with 5 x 10(-8) M estradiol-17 beta or progesterone in TC medium 199 containing 10% calf serum at 37 degrees C for 1 hr. The immunofluorescent steroid-antibody technique using FITC-labeled anti-rabbit IgG and steroid antibodies raised from rabbits immunized with estradiol-6-oxime-BSA and progesterone-3-oxime-BSA was applied to the smear specimens and freeze-dry sections that had been incubated with these steroids. In 6 cases, there was a mixed cell-population with or without nuclear fluorescence to estradiol and progesterone. The cellular heterogeneity with respect to nuclear fluorescence was confirmed by the immunofluorescent technique applied to the freeze-dry sections. Two cases showed weak cytoplasmic fluorescence to estradiol and progesterone, but nuclear fluorescence was negative. In 3 cases, there was no immunoreactivity to these steroids. One case exhibited the similar distributional patterns of cytoplasmic and nuclear fluorescence to those of normal endometrial cells. These results indicated that in endometrial cancer, there were some impairments of subcellular steroid dynamics in comparison with those of normal cells and that cellular heterogeneity of the steroid reactivity might exist in some cases.

Adult↗
PubMed1979

Gray scale transrectal ultransonography of the prostate.

Gray scale transrectal ultrasongraphy provides refined visualization of the texture of the prostate and other intrapelvic organs. Diagnostic accuracy of the transrectal scan has been successfully registered at 91% in benign prostatic hyperplasia, 85% in cancer, 89% in prostatitis, and 88% in total prostatic scans. Furthermore, prostatic calculi were visible in the tomograms of both normal and abnormal prostates at a higher frequency than generally considered. Other applications of ultrasonic control, such as during transurethral resection or cryosurgery of the prostate and staging of bladder tumors, are profitable using the gray scale technique.

Calculi↗
PubMed1979

Combined protein and DNA measurements by the ninhydrin-Schiff and Feulgen techniques.

Feulgen nuclear staining with pararosanilin-SO2 was combined with the ninhydrin-Schiff technique. The aldehyde groups converted from primary amino groups are stained with an acriflavine-Schiff reaction. This results in a red nuclear fluorescence and a bright yellow cytoplasmic and nuclear fluorescence. The combined fluorescence staining facilitates cytofluorometric determination of total protein and DNA in the same cell. The ninhydrin-Schiff reaction is affected by the fixation procedure and the duration of the ninhydrin reaction. Investigations with a model system showed that proportionality between the fluorescence intensity of acriflavine and the amount of protein stained by the procedure was obtained after fixation with a fixation mixture suggested by Böhm et al. (1968) and a reaction with ninhydrin at 37 degrees C for 10 h. The ninhydrin-Schiff reaction has no effect on the fluorescence intensity of cells previously treated with pararosanilin-Feulgen staining and it is not affected itself by this previous procedure. Testing this double fluorescence staining on cytology specimens taken from patients with gastric carcinoma and uterine cervial carcinoma, cancer cells were shown to have markedly increased protein and DNA contents compared with those of normal cells.

Adult↗