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Biomedical subjects

K Hara

Publications and source records attributed to K Hara.

At least 1,081 records · Page 60Linked to original sources
PubMed1983

[Fetal motor activity and acceleration in the cardiotocogram].

The results of the concomitant monitoring of the fetal heart rate and the total fetal movement activity by two ultrasound monitors are reported. In 111 patients from 26 weeks gestation to term the cardiotocogram and the fetal movement activity were at the same time monitored with two ultrasound monitors. In total 453 fetal heart rate accelerations of more than 15 beats per minute and more than 15 seconds duration were found in all cardiotocograms. The most frequent finding with an acceleration of the fetal heart rate were fetal body movements with or without movements of the extremities in 71% of the fetal heart rate accelerations. In 18% of the fetal heart rate accelerations movements of the fetal extremities were found by ultrasonography. In 7% of the fetal heart rate accelerations, fetal respiratory movements or contractions were found without fetal body movements. 4% of the fetal heart rate accelerations occurred without sonographically or topographically recognizable cause. In all ultrasonographically recognized fetal movements of the body and/or the extremities the fetal heart rate accelerations occurred in 28% of the cases. The most frequent findings were movements of the fetal body with or without movements of the fetal extremities in 48% of the fetal heart rate accelerations. In isolated movements of the fetal extremeties the fetal heart rate was only accelerated in 11% of the cases.

Female↗
PubMed1983

[Detection of fetal movements in the tocogram].

The total fetal movement activity was determined in 111 pregnant patients from 24 weeks to 42 weeks by simultaneous real-time ultrasonography and tocodynamometry. A comparison of the methods of investigation showed that tocodynamometry only detects 37% of all fetal movements with or without movements of the extremities. Movements of the extremities were only recorded in 14% of the cases. A total of only 25% of all fetal movements showed "spikes" in the tocogram. Of all the recorded spikes only 56% corresponded to a fetal movement, whereas 44% of the spikes in the tocogram were false-positive. These data demonstrate that routine clinical cardiotocography has a limited value only in the monitoring of fetal movement activity.

Female↗
PubMed1983

Congenital hypomyelination neuropathy in a newborn.

A case of infantile neuropathy with signs of respiratory distress and impaired abdominal movements from the neonatal period was reported. Muscle biopsy revealed type II fiber predominance and atrophy, and the sural nerve biopsy demonstrated the absence of myelin in almost all of the axons. Onion bulb formation or myelin debris was not observed. The features of myelin deficit without active myelin breakdown and onion bulb formation, and the finding of muscle pathology, indicating the absence of spinal muscular atrophy, suggested that the case under study was identical to that of hypomyelination neuropathy.

Axons↗
PubMed1983

Purification and characterization of a calcium-activated neutral protease from monkey cardiac muscle.

A calcium-activated neutral protease (CANP) was purified from monkey cardiac muscle by a method involving column chromatography on DEAE-cellulose, Sepharose CL-6B, DEAE-Sephacel, organomercurial-Sepharose 4B, and Sephadex G-150 in succession. This protease required both millimolar concentration of Ca2+ and the SH-group for activation, and it was maximally active around pH 8.0. It was strongly inhibited by thiol protease inhibitors such as iodoacetic acid, antipain, leupeptin, and epoxysuccinic acid derivatives. The molecular weight of this protease was estimated to be 110,000 by gel filtration. Upon nondenaturing electrophoresis the purified protease gave two bands, both of which were active at millimolar concentration of Ca2+, indicating the existence of two forms of the protease. The less acidic band (form I CANP) contained two components with molecular weights of 74,000 and 28,000 and the more acidic one (form II CANP) contained components with molecular weights of 74,000 and 26,000. The protease was synergistically activated by Mn2+ and Ca2+ at a concentration where Mn2+ or Ca2+ alone was not effective. In the presence of millimolar level of Ca2+, limited autolysis reduced the Ca2+-requirement of this protease. The proteolysis of myofibrils by this protease resulted in the production of a component with a molecular weight of 30,000 as well as various other higher and lower molecular weight peptide fragments.

Animals↗
PubMed1983

Degradation of neuropeptides by calcium-activated neutral protease.

The substrate specificity of calcium-activated neutral protease (CANP) from monkey cardiac muscle was examined with various neuropeptides as substrates. The enzyme required mM order calcium ions for activation and had an enkephalinase activity, hydrolyzing Leu-enkephalin at the 1Tyr-2Gly and 3Gly-4Phe bonds. Furthermore, it showed the tendency to cleave especially the bonds around the paired basic amino acid residues in alpha- and beta-neoendorphins and dynorphin(1-13), while it could not hydrolyze substance P.

Amino Acid Sequence↗
PubMed1983

High density bile in the gallbladder observed by computed tomography.

In eight of 620 computed tomographic examinations of the gallbladder the density of bile was higher than that of the liver. In three the bile density increased and in one it decreased on serial studies. Four of these eight patients had hepatocellular carcinoma.

Absorptiometry, Photon↗
PubMed1983

New beta-lactamase-resistant cephem treatment of guinea pigs infected with Legionella pneumophila.

The in vivo antimicrobial effect of seven new beta-lactamase-resistant cephems (cefotaxime, latamoxef, ceftazidime, ceftriaxone, cefotiam, cefbuperazone, and MT-141) on Legionella pneumophila (strain 81-066, serogroup IV) in guinea pigs was compared with that of erythromycin. As the minimal LD100 within one week was about 4.0 X 10(9) CFU/ml by intraperitoneal injection of the strain, the animals were inoculated with 2.0 ml of twofold dilutions of a suspension of this bacterium. The animals developed purulent peritonitis and systemic involvement demonstrated by the development of periangitis, pneumonia and pleuritis in the lungs. Three different doses of antibiotics were administered intraperitoneally immediately after the rectal temperature reached more than 40 C. Erythromycin had a significant therapeutic effect but none of the new cephems tested death of the infected guinea pigs.

Animals↗
PubMed1983

Transformation of human embryonic kidney cells by a viable deletion mutant of BK virus.

Infection by a viable deletion mutant of BK virus (pm-522) of human embryonic kidney (HEK) cultures originating from different fetuses generated cell lines, designated pmHEK, resistant to superinfection by the virus. However, infection of HEK cultures by a cloned wild-type BK virus (wt-501) did not generate a cell line. In medium with 10% calf serum all pmHEK cells reached saturation densities significantly higher than those of HEK cells and could grow in medium containing 1% serum. They did not form colonies in soft-agar medium, and had limited life-spans greatly extended beyond that of HEK cells. These results suggest that pmHEK cells are partially transformed. T antigen was uniformly expressed by all pmHEK cells, while V antigen was present in only a small minority of the cells. Thirty to 5,000 copies of the viral DNA per cell were detected, primarily in a nonintegrated form, in all pmHEK cell lines and the clones isolated from one of them. Since at least in two pmHEK lines, the actual quantities of free viral DNA per cell were significantly greater than those estimated on the basis of the assumption that free viral DNA was produced only by the V-antigen-positive cells, it can be concluded that free viral DNA is also present in the V-antigen-negative cells predominant in the cell populations. Although the existence of a few copies of integrated viral sequences in pmHEK cells was not ruled out, the large amount of free viral DNA present in each cell probably plays a prominent role in the production of T antigen required for maintenance of transformation.

Animals↗
PubMed1983

The prognostic value of EEG in neonatal meningitis.

EEG recordings were made polygraphically in 29 newborn infants with meningitis. Visual and auditory evoked potentials, and CT scans were also obtained in some of them. The neonatal findings of these examinations were well correlated with the clinical outcome. Background EEG activity was a good prognostic tool but could not indicate the type of complications, although persistent abnormality correlated with severe brain damage or development of complications. The polygraphically recorded EEG was of great value to diagnose the apneic seizures often encountered in neonatal meningitis.

Alpha Rhythm↗