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Biomedical subjects

K Goh

Publications and source records attributed to K Goh.

At least 73 records · Page 4Linked to original sources
PubMed1983

Erythroleukemia manifesting delta beta-thalassemia.

A 27 year old male with aplastic anemia developed a high fetal hemoglobin, a low hemoglobin A2, a decreased beta/alpha synthetic ratio, and an increased G gamma/A gamma synthetic ratio. This acquired hemoglobinopathy resembling delta beta-thalassemia was recognized at the onset of acute erythroleukemia. Certain features of this abnormal globin synthetic pattern resemble those of the normal fetus and thus appear to provide another example of gene expression by malignant cells resembling that of an earlier stage of the organism's development.

Adult↗
PubMed1981

Chromosomal aberrations in leukemic cats.

The bone marrows of two female acute lymphoblastic leukemic cats were studied cytogenetically with the conventional and one also with the G-banding techniques. Both cats had marked aneuploidies. Cat leukemia is caused by virus, FeLV. Perhaps, this virus is capable of affecting cat chromosomes and as a result of this process converts the normal cells into leukemic cells.

Animals↗
PubMed1980

Chromosomal abnormalities. Findings in a patient with lymphoma and rheumatoid arthritis treated with intra-articular gold Au 198.

Cytogenetic studies from the peripheral blood of a patient with malignant lymphoma and rheumatoid arthritis who was treated with intra-articular gold Au 198 revealed mosaicism with a normal female metaphase and a 43-chromosome metaphase. The abnormal cell line showed six missing normal chromosomes and three morphologically abnormal chromosomes. The trypsin-digested G-banding metaphases showed that the marker chromosomes were an isochromosome of the long arm of chromosome 17, a translocated chromosome that involved the long arm of chromosome 4 and a chromosome 16, and a translocated chromosome that involved the long arm of chromosome 4 and a chromosome 5. It is tempting to conclude that these abnormalities were due to the gold Au 198 treatment, but we cannot exclude other possibilities.

Aged↗
PubMed1979

Chloramphenicol and chromosomal morphology.

Eight-hundred mcg of chloramphenicol (CM) was added to 10 ml of normal phytohemagglutinin-stimulated leukocyte cultures at various stages of cell cycle. The diluent used to dissolve CM was added to the control cultures. The cultures were terminated for chromsomal analyses. More abnormalities were seen in the CM added cultures. The highest incidence was seen when CM was added at G0 and the lowest, at G2. These parallel well with the acidic residual chromosomal protein fraction in HeLa S-3 cells. It was concluded that CM is capable of inhibiting protein synthesis resulting in chromosomal protein deficiency. This deficiency produces "weakness" in the chromosomal backbone.

Cells, Cultured↗
PubMed1979

Chromosomal abnormalities in maintenance hemodialysis patients.

Forty bone marrow aspirates from maintenance hemodialysis patients were prepared for chromosomal analyses. Among the 1,187 metaphases studied, only 58% were diploidies. Other abnormalities included: pseudodiploidies (5.8%), acentric fragments (3.6%), translocated chromosomes (1.9%) and one dicentric chromosome. The abnormalities found may be due to abnormal DNA synthesis in chronic renal failure or uremia, or infusion of formaldehyde which can denature DNA. This chemical was used to sterilize dialysis equipment. Incomplete rinsing can introduce this substance into the circulation.

Adolescent↗
PubMed1978

Down's syndrome and leukemia: mechanism of additional chromosomal abnormalities.

There is an increased incidence of acute leukemia in patients with Down's syndrome patients have a trisomy-21 chromosomal pattern, and chromosomal abnormalities can be seen in acute leukemia. It is possible that the increased incidence of acute leukemia in Down's syndrome persons may be due in part to their chromosomal abnormalities. Such abnormalities, some appearing in a stepwise clonal evolution, were found in five Down's syndrome patients, four with acute leukemia and one with abnormal regulation of leukopoiesis. Morphological abnormal chromosomes were also found in three patients. These chromosomal abnormalities are similar to those seen in non-Down's syndrome leukemic patients. There is suggestive evidence for clonal evolution hypothesis of luekemogenesis in non-Down's syndrome patients. The abnormal chromosomal pattern reported in our Down's syndrome patients could be the result of nondisjunction in mitosis, and leukemia may be the phenotypical expression of this nondisjunction.

Acute Disease↗
PubMed1977

In vivo leukemic transformation: cytogenetic evidence of in vivo leukemic transformation of engrafted marrow cells.

A 4-year-old girl with acute myeloblastic leukemia was treated with 50 mg/kg cyclophosphamide daily for 4 days before being given 1.7 X 10(8) bone marrow cells/kg from her HL-A identical, MLC nonreactive, cytogenetically normal brother. The patient died 92 weeks after the marrow graft. Postmortem examination showed increased myeloblasts and promyelocytes. Cytogenetic studies before transplantation showed that all sex determination metaphases had an XX pattern, and 41% of the hyperdiploid metaphases had an additional 19-20(F) chromosome. At autopsy all hyperdiploid metaphases with XY pattern and 43% of the hyperdiploid metaphases with an XX pattern had an additional F chromosome. Occasional metaphases with 47, XX, + F or 47, XY, + F were seen during the follow-up studies. These findings indicated that an acute leukemia had developed in the XY cell line of this artifically induced sex chimeric child. This suggests that a leukemic stimulus other than that proposed to be induced by total-body irradiation existed in this patient and transformed the engrafted cells.

Bone Marrow↗
PubMed1976

B and T lymphocytes in man. II. Circulating B and T lymphocytes in cancer patients.

B, T and "Null" lymphocytes from 87 coded peripheral blood samples from 33 normals and 54 nonoperable or progressive cancer patients were determined. The controls have higher mean numbers of circulating lymphocytes than the cancer patients. Radiation-treated cancer patients have lower values than non-irradiated patients. This phenomenon is also seen in the relative and absolute T, but not the B lymphocytes. There was no difference in the B lymphocytes among the different groups of patients. However, the controls have the highest and the radiation-treated cancer patients have the lowest absolute numbers of B lymphocytes. Although cancer patients have higher relative numbers of "Null" cells than the controls, only the difference between the controls and the radiation-treated cancer patients was statistically significant.

Adult↗
PubMed1975

Double aneuploidy. Turner-Down syndrome.

Double aneuploidy involving Down and Turner syndromes is a rare occurrence. Of the six patients reported to have combined Down and Turner syndromes, four fundamentally different forms of chromosome mosaicism have been noted and all have been mosaic with respect to monosomy X. Reported here is the first example of a Turner-Down patient in whom there is no X mosaicism. The different forms of the double aneuploidy cannot be explained by any single combination of nondisjunctional errors. The clinical findings in these patients and the several mechanisms of nondisjunctional error that may account for the observed forms of aneuploidy are reviewed and discussed.

Aneuploidy↗
PubMed1975

Phytohemagglutinin committed lymphocytes: the mitotic activity of the phytohemagglutinin stimulated lymphocytes.

The purpose of these experiments was to find out why there was a decrease in the mitotic activity in the prolonged PHA stimulated lymphocyte cultures. The present observations suggested that the decrease in the mitotic rate in the prolonged PHA-stimulated lymphocytes was not due to a deficiency in the essential material in the culture medium to sustain a maximum mitotic response, nor was it due to an accumulation of the metabolites that might inhibit the lymphocytes to undergo further division. However, the results obtained suggest that there may be a limitation in the number of generation cycles in which the T lymphocytes can be stimulated with the PHA. They also suggest that a second dose of PHA may stimulate some of the original noncommitted lymphocytes to undergo mitosis thus attempting to maintain the in vitro lymphocyte population.

Female↗